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Citations for Agilent :
7451 - 7500 of 8085 citations for Rat Nuclear Factor Of Activated T Cells 5 NFAT5 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... The exome region of the whole genome libraries was enriched with the Agilent SureSelectXT Human All Exon Kit V6 (Agilent; Santa Clara, CA) and sequenced on the NovaSeq 6000 Sequencing System (Illumina ...
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bioRxiv - Genomics 2022Quote: ... we assessed the quality of the RNA by quantifying the RNA integrity number (RIN) for each sample using the Agilent RNA 6000 Pico kit (Agilent, Santa Clara, CA) on the Agilent 2100 Bioanalyzer (Agilent ...
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bioRxiv - Cell Biology 2023Quote: ... The human GABAA receptor α1 subunit missense mutations (S76R, R214C, D219N, G251D, P260L, M263T, T289P, and A322D) were constructed using QuikChange II site-directed mutagenesis Kit (Agilent Genomics, catalog #: 200523). Enhanced cyan fluorescent protein (CFP ...
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bioRxiv - Molecular Biology 2023Quote: ... The purified DNA library was analyzed using an Agilent 2100 bioanalyzer with an Agilent DNA 1000 Kit (Agilent Technologies, Santa Clara, CA, USA) and a Qubit 2.0 with Qubit 1X dsDNA HS Assay Kits (Thermo Fisher Scientific ...
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Epigenetic deregulation of IFN and WNT pathways in AT2 cells impairs alveolar regeneration (in COPD)bioRxiv - Cell Biology 2023Quote: ... RNA concentration and integrity were measured using the RNA Pico 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA), and only samples with RIN > 8 were included in RNA-seq ...
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bioRxiv - Physiology 2023Quote: ... and the quality (e.g. fragment length) was assessed for a subset using a Bioanalyzer High Sensitivity DNA chip kit (Agilent Technologies, Santa Clara, CA). Single-end sequencing with 100-bp read length was conducted on a NovaSeq platform (Illumina ...
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bioRxiv - Molecular Biology 2023Quote: ... Quality of the RNA samples (RIN score >7) was verified on the Agilent 2100 Bioanalyzer using the RNA 6000 kit (Agilent, cat# 5067-1513). 1 µg of each RNA sample was then used for poly(A ...
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bioRxiv - Neuroscience 2023Quote: ... Beads-based double size selection was performed to ensure the fragment size of each sample was between 300-600 bp as measured by an Agilent DNA High Sensitivity NGS Fragment Analysis Kit (Agilent, DNF-474-0500). The concentration of ligated fragments in each library was quantified with the KAPA Library Quantification Kits for Illumina platforms (Roche/KAPA Biosystems ...
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bioRxiv - Molecular Biology 2023Quote: ... quantitation and sample purity were assessed using the RNA Nano 6000 Assay Kit of the Agilent Bioanalyzer 2100 system (Agilent Technologies, CA, USA) and agarose gel electrophoresis ...
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bioRxiv - Cancer Biology 2023Quote: ... The oxygen consumption rate (OCR) assay was performed using the XF long-chain fatty acid oxidation (LCFA) stress test kit (Agilent, cat# 103672-100) and Seahorse XFe24 Analyzer was used to investigate the long-chain fatty acid OCR according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2023Quote: ... and leucine (Leu41) within the conserved destruction box were mutagenized to alanine (RxxL-> AxxA) using the Quickchange mutagenesis kit (Stratagene, La Jolla, CA). Worms were transformed with the pJK3 plasmid carrying gfp::ify-1dm using microparticle bombardment as previously described (Praitis et al. ...
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bioRxiv - Cell Biology 2023Quote: ... Total amounts and integrity of RNA were assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). Total RNA was used as input material for RNA sample preparations ...
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bioRxiv - Developmental Biology 2023Quote: ... We performed exome sequencing in the child with diabetes and his consanguineous parents and sibling using the Agilent SureSelect Human All Exon Kit (Agilent SureSelect v4, 50Mb) and next-generation sequencing (Hiseq ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA concentration and quality was measured using the RNA 6000 Pico kit on the Bioanalyzer 2100 capillary electrophoresis system (Agilent, Santa Clara, CA) and stored at −80°C ...
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bioRxiv - Genetics 2024Quote: ... poly(A)+ RNA was separated and purified from total RNA using an oligo(dT) cellulose column Poly(A) Quick mRNA Isolation Kit (Stratagene, La Jolla, CA). Single-stranded cDNAs were synthesized by reverse transcription reaction using 0.25 U AMV reverse transcriptase (Life Sciences Advanced Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... while the concentration and integrity of RNA samples were detected by using Agilent 2100 RNA nano 6000 assay kit (Agilent Technologies, CA, USA). Subsequently ...
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bioRxiv - Biophysics 2024Quote: Introduction of the 118C mutation into the T4L gene (pET11a vector) was accomplished using the QuikChange site-directed mutagenesis kit (Agilent; Santa Clara, CA) and confirmed by DNA sequencing ...
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bioRxiv - Cell Biology 2024Quote: ... Mutations were constructed by site-directed mutagenesis according to the instructions accompanying the Quik Change ® Site-Directed Mutagenesis Kit (Agilent Technologies, Stratagene). Conventional PCRs reactions were performed with KAPA Taq DNA polymerase (Kapa Biosystems ...
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bioRxiv - Biochemistry 2024Quote: ... Average fragment lengths of 40-50 kbp were confirmed using a Fragment Analyzer (Advanced Analytical) with an HS Large Fragment 50 Kb analysis kit (Agilent, DNF-464-33). Samples were prepared for Oxford Nanopore Technologies (ONT ...
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bioRxiv - Microbiology 2024Quote: ... To determine the RNA integrity number the isolated RNA was run on an Agilent Bioanalyzer 2100 using an Agilent RNA 6000 Nano Kit as recommended by the manufacturer (Agilent Technologies, Waldbronn, Germany). Remaining genomic DNA was removed by digesting with TURBO DNase (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... Library qualities were assessed using the Agilent High Sensitive DNA Kit (#5067-4626) on an Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, USA). Qualified libraries underwent paired-end sequencing on an Illumina NovaSeq System (Illumina ...
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bioRxiv - Cell Biology 2024Quote: ... a DeNovix DS-11 FX spectrophotometer and an Agilent FEMTO Pulse System with a Genomic DNA 165 kb Kit (Agilent, FP-1002-0275), respectively ...
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bioRxiv - Cell Biology 2024Quote: ... and RNA integrity was assessed using the Agilent TapeStation 4200 and the Agilent RNA ScreenTape Assay Kit (Agilent; 5067-5576 and 5067-5577).
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bioRxiv - Plant Biology 2024Quote: ... The quality of the barcoded cDNA libraries was assessed with the High Sensitivity DNA Analysis Kit in the Agilent 2100 Bioanalyzer (Agilent Technologies, United States) and then sequenced (150 bp paired-end reads ...
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bioRxiv - Genetics 2024Quote: ... RNA concentration and quality was assessed using an Agilent 2100 Bioanalyzer with the RNA Nano 6000 kit (Agilent Technologies, Santa Clara, CA, USA), and only RNA samples with RIN >7 were processed for subsequent cDNA library construction and sequencing ...
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bioRxiv - Cancer Biology 2021Quote: 1×104 OE19 cells were reverse transfected with siRNAs and seeded into 96-well plates (Agilent, 102601-100). 24 hours post-transfection cells were treated with 500 nM lapatinib or vehicle control ...
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bioRxiv - Microbiology 2022Quote: ... Mid-log phase fungal cells grown in YNB medium +/- 80 μg/mL benomyl (Agilent catalog no. PST-1245) for 24 hours were used to set 1C and 2C gates ...
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bioRxiv - Genomics 2019Quote: ... Fragment size and integrity of amplified single-cell genomes were analyzed using Bioanalyzer High Sensitivity DNA Assay (Agilent) with Agilent Bioanalyzer 2100 (2100 Expert B.02.08.S648 SR3 ...
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bioRxiv - Biochemistry 2020Quote: Wild-type (WT) and F94S His6-CSAD were expressed in Escherichia coli BL21 CodonPlus (DE3)-RIPL cells (Stratagene) at +15 °C using 0.5 mM IPTG induction ...
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bioRxiv - Cell Biology 2021Quote: ... cells were resuspended in DMEM with 10% of FCS and transferred into xCELLigence microplates (E-plate 16, Agilent) at the density of 20 000 cells for real-time analysis of cell adhesion and growth ...
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bioRxiv - Cell Biology 2021Quote: ... The pST39-BLOC-1 plasmid encoding recombinant BLOC-1 was transformed into BL21gold(DE3)plysS cells (230134, Agilent). Several colonies from the plate were inoculated into a starter culture of 10 ml LB supplemented with 34 μg/ml chloramphenicol and 100 μg/ml ampicillin that was grown overnight at 37 °C with moderate shaking ...
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bioRxiv - Cancer Biology 2020Quote: ... pre-coated XF96 plates at 105 cells/well in 175 μL of sodium bicarbonate-free XF Base (Agilent) media freshly supplemented with 10 mM glucose ...
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bioRxiv - Cell Biology 2021Quote: Respiration and acidification rates were measured in cells using a Seahorse XF24 analyzer (Seahorse Bioscience, North Billerica, MA). Briefly ...
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bioRxiv - Biochemistry 2022Quote: ... and extracellular acidification rate were determined following the manufacturer’s instructions for the Cell Mitochondrial Stress Test (Agilent Technologies) and normalized to total cell count.
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bioRxiv - Immunology 2022Quote: ... Cells were washed twice and incubated with LPS (100 ng/mL) in XF based medium (Agilent, 103334-100) supplemented with 4.5 g/L glucose ...
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bioRxiv - Molecular Biology 2022Quote: ... Validation of the single-cell library is conducted using the Agilent Tapestation 4200 ScreenTape assay (Agilent 5067-5576). Single-cell library quantification is completed using a Roche LightCycle96 using FastStart Essential Green Master (Roche 06402712001 ...
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bioRxiv - Molecular Biology 2022Quote: ... a T7 endonuclease assay was performed on NIH3T3 cells and the cleavage efficiency was analyzed using TapeStation (Agilent). Two gRNAs (1 and 4 ...
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bioRxiv - Cell Biology 2022Quote: ... MZ and MM iHeps were seeded onto 96 well Matrigel coated Seahorse XF Cell Culture Microplate (Agilent Technologies) at a density of 50,000 cells/well ...
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bioRxiv - Microbiology 2020Quote: ... coli Lgt fused to a C-terminal Flag-tag was transformed into Rosetta 2(DE3) Gold cells (Agilent). Starter cultures were grown in Terrific Broth (TB ...
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bioRxiv - Neuroscience 2020Quote: ... AAV9 viruses for the reactivation experiment were produced by CaPO4 co-transfection of AAV293 cells (Stratagene, La Jolla) with the pAAV-cFos::tTA or pAAV-TRE::mCherry (gifts from William Widsen ...
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bioRxiv - Microbiology 2022Quote: ... Plasmids were then transformed into XL-10 Gold chemically competent Escherichia coli cells (Agilent Technologies, Santa Carla, CA) as previously described [14] ...
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bioRxiv - Immunology 2022Quote: BAL fluid of two mice was pooled and 50.000 – 100.000 cells were plated in a 96 well Seahorse plate (Agilent) in Seahorse XF base medium (Agilent ...
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bioRxiv - Genetics 2022Quote: ... The cells were UV irradiated with 400mJ/cm2 at 254 nm using the UV Stratalinker crosslinker 1800 (STRATAGENE). The crosslinked cells were collected with a scraper and a pellet was obtained ...
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bioRxiv - Cancer Biology 2022Quote: ... Cartridge was placed onto microplate containing cells and inserted into a Seahorse XF-24 Flux Analyzer (Agilent Technologies). Three measurements of 2 min-mix ...
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bioRxiv - Neuroscience 2019Quote: ... Cell nuclei were stained with Hoechst (1:10000) and the coverslips were mounted with DAKO fluoromount medium (Agilent). The primary antibodies were used ...
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bioRxiv - Pathology 2020Quote: ... The self-complementary scAAV9-CB-SMN vector was produced by calcium phosphate transfection of HEK293-AAV cells (Agilent) with pAAV-CB-SMN[57] and pDF9 plasmids ...
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bioRxiv - Physiology 2019Quote: ... cells were subjected to a Seahorse XF Mito Stress Test using the manufacturer’s instructions (Agilent, Santa Clara, CA). Cells were seeded at 30,000 per well in 24 well ...
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bioRxiv - Physiology 2021Quote: ... seeded for culture (2500-7000 cells per well) in Seahorse XFp Miniplates (Agilent, Santa Clara, CA, see below) or 96-well culture plates and then differentiated into mature adipocytes according to minor modifications of our previously published protocols (Gehring et al. ...
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bioRxiv - Neuroscience 2021Quote: ... Hoechst 33342 was used to stain the nucleus before cells were mounted with fluorescent mounting medium (DAKO, #S3023). The following primary antibodies were used ...
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bioRxiv - Biochemistry 2021Quote: The second set of experiments was performed on Seahorse XF Cell Culture Microplates (Agilent, cat. n. 103022-100) with eight wells ...