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Citations for Agilent :
701 - 750 of 762 citations for Poloxamer 188 solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... was performed on 4-μm thick formalin-fixed paraffin-embedded (FFPE) whole tissue serial sections following antigen retrieval (Target Retrieval Solution, pH 6.1; Dako, Carpinteria, CA). Immunostains performed included Connexin 26 (Cx26 ...
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bioRxiv - Biophysics 2023Quote: ... BSA and urease stock solutions concentration are measured after their preparation using a UV-vis spectrophotometer (Cary 60 Spectrophotometer, Agilent Technologies). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: ... Antigen retrieval was performed by heating slides in a pressure cooker for 45 minutes with Target Antigen Retrieval Solution (Dako, S1699). Sections were blocked with 10% goat serum for 30 minutes and primary antibodies were incubated at 4°C overnight ...
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bioRxiv - Bioengineering 2023Quote: ... using a 1.5% w/v alginate solution in deuterium oxide (64 scans; Agilent 400 MHz Premium COMPACT equipped with Agilent OneNMR Probe) and analyzed using MestrNova Software (14.6 ...
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bioRxiv - Biophysics 2023Quote: ... The monodisperse sample solutions of proteins were injected onto a size exclusion column (David and Perez 2009) (SEC-3, 150 Å; Agilent) using an Agilent HPLC system and eluted into the capillary cell at a flow rate of 0.3 ml min-1 ...
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bioRxiv - Cancer Biology 2023Quote: The 4-6um thick TMA sections were deparaffinized and antigens were unmasked using EnVision FLEX Target Retrival Solution Low pH (Dako Agilent) in PT link device (PT200 ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were fixed for 15 min in 4% paraformaldehyde and blocked for 60 min in a blocking solution (DAKO, Ontario, Canada). Sections were incubated overnight in a polyclonal rabbit anti-IBA-1 primary antibody (1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... The Seahorse XFp extracellular flux cartridge was hydrated with sterile water and incubated overnight at 30 °C and Seahorse XF calibrant solution (Agilent Technologies). After this ...
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bioRxiv - Bioengineering 2023Quote: ... The electrode temperature was controlled by submerging in 70 °C PBS solution with a P4010 thermal meter (Dostmann electronic, Germany) for temperature monitoring and a 34401A digit multimeter (Agilent, USA) for electrical resistance measurement ...
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bioRxiv - Bioengineering 2024Quote: ... The pellets from centrifugation were dried under vacuum and re-dissolved using 5% acetic acid aqueous solution for purification by HPLC (1260 Infinity, Agilent Technologies) equipped with a C8 column (Zorbax 300SB-C8 ...
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bioRxiv - Biochemistry 2024Quote: ... A reference mass correction solution was continually infused at a flow rate of 0.5 mL/min via an external isocratic pump (Agilent, Cheadle, UK) for constant mass correction (see preparation of reference mass correction solution) ...
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bioRxiv - Bioengineering 2024Quote: ... then gradually increase the pH by adding NaOH (aq.) to the peptide solution and monitor the turbidity changes by spectrophotometer at the wavelength of 600 nm (Agilent carry3500). When a turbidity appeared ...
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bioRxiv - Physiology 2024Quote: ... the cell pellet was resuspended in 500ul of warm MAS buffer solution [58] + 1nM Plasma Membrane Permeabilizer (Agilent Seahorse XF PMP) to permeabilize the cells ...
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bioRxiv - Bioengineering 2024Quote: ... using a 1.5 % w/v alginate solution in deuterium oxide (64 scans; Agilent 400 MHz Premium COMPACT equipped with Agilent OneNMR Probe) and analyzed using MestreNova Software (version 12.03).
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bioRxiv - Immunology 2024Quote: ... the formalin-fixed paraffin-embedded human PDAC tissue sections were deparaffinized by Xylene and gradient alcohol solutions and stained with hematoxylin (S3301, Dako, US) for 1 min ...
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bioRxiv - Developmental Biology 2021Quote: Immunofluorescence microscopy was performed on de-paraffinized tissue sections that received heat-induced antigen retrieval using Target Retrieval Solution (Agilent Dako, S169984-2). After washing and permeabilization in TBS-T universal buffer (0.2% Triton X-100 in tris-buffered saline) ...
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bioRxiv - Cancer Biology 2021Quote: ... 4 μm FFPE tissue sections were cut followed by an antigen retrieval by heat treatment with Target Retrieval Solution (S1699, Agilent Technologies, Germany). Slides were incubated with a monoclonal anti-γH2A.X (phosphor-S139 ...
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bioRxiv - Neuroscience 2021Quote: ... or dewaxed as part of the antigen retrieval process using the Low pH EnVision ™ FLEX Target Retrieval Solutions (K8005, Dako-Agilent) for 20 min at 97°C using a PT Link (Dako – Agilent) ...
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bioRxiv - Neuroscience 2021Quote: ... The slides were washed three times (3X) in TBST and incubated in blocking solution (Protein Block Serum-Free, Agilent DAKO, CA, USA) for 30 min at room temperature (RT) ...
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bioRxiv - Neuroscience 2021Quote: ... The slides were washed three times (3X) in TBST and incubated in blocking solution (Protein Block Serum-Free, Agilent DAKO, CA, USA) for 30 min at room temperature (RT) ...
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bioRxiv - Physiology 2021Quote: ... Primary antibodies are diluted in 1x blocking solution and incubated with antibodies against insulin (1:2000, guineapig, Dako, cat. no. A-0546), glucagon (1:500 ...
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bioRxiv - Cancer Biology 2021Quote: ... Tissues were treated with Protein Block Serum-Free solution (Protein Block Serum-Free Ready-to-Use, catalog no. X0909, Dako, Glostrup, Denmark) for 30 min at room temperature ...
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bioRxiv - Immunology 2020Quote: ... Sections were deparaffinized by pretreatment with 0.5% H2O2 in methanol and then subjected to antigen retrieval with target retrieval solution (Dako S1700, pH 6.1) followed by heating in an autoclave for 20 min at 121 °C ...
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bioRxiv - Neuroscience 2022Quote: ... The sections were rinsed in PBS (2 × 10 min) before antigen-retrieval by incubation in target retrieval solution (Dako S1700, Glostrup, Denmark) for 40 min at 85 °C ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... A 470 µL aliquot of pre-mixed solution without TQ was transferred to a 96-well plate (Agilent Technologies, Santa Clara, USA) three wells (n = 3) ...
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bioRxiv - Cancer Biology 2024Quote: ... antigen retrieval was performed using target retrieval solution pH 6.0 or pH 9.0 according to antibody protocol recommendations (Agilent, Santa Clara, CA, USA). The samples were scanned (panoramic slide digital scanner ...
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bioRxiv - Physiology 2023Quote: ... slides (4 μm thickness) were baked at 60° C for 45 min and treated with a low pH target retrieval solution (#DM829; Dako, Glostrup, Denmark) in a DAKO PT Link HIER machine (PTlink 200 ...
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bioRxiv - Bioengineering 2023Quote: ... The clarified aqueous solution was filtered using a 0.22 µm filter and run on HPLC-RID (Agilent 1290 Infinity II/Agilent 1100) for glucose measurements ...
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bioRxiv - Cancer Biology 2023Quote: ... treated with 3% H2O2 to block endogenous peroxidase then incubated in a steamer for 30 minutes in Target Retrieval Solution (Dako, #S1699, #S2367) for antigen retrieval ...
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bioRxiv - Cancer Biology 2023Quote: ... the cells were permeabilized with 0.1% Triton x-100 (Fluka, 93426) and blocked with the blocking solution containing 3% Goat serum (Dako, Cat. no. X0907), 0,1% sodium azide (the healthcare business of Merck KGaA ...
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bioRxiv - Biophysics 2024Quote: ... that is loaded onto a Waters HDX-Manager with a desalting flow of 0.23% aqueous formic acid solution (Solvent A) provided by an Agilent 1260 Infinity quaternary pump (Agilent Technologies, CA, USA) and a gradient flow comprised of Solvent A (0.23% aqueous FA solution ...
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bioRxiv - Cell Biology 2024Quote: ... The concentration of H2O2 stock solution was determined at 240 nm in an UV-Vis spectrophotometer (Cary 60, Agilent Technologies, CA, USA), considering a molar extinction coefficient of 43.6 M−1cm−1 ...
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bioRxiv - Neuroscience 2024Quote: ... Wildtype mouse brain paraffin sections were used for IHC analysis with a dilution of mouse serum at 1:200 with antibody diluent solution (DAKO, S080983-2). Mouse anti-NMDAR1 monoclonal antibody (BD ...
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bioRxiv - Immunology 2024Quote: ... The antigens on the slides were retrieved by submerging slides in 3-in-1 Target Retrieval Solution (pH 9, DAKO Agilent, Cat# S2375) and incubating at 97 °C for 40 min in a Lab Vision PT Module (Thermo Fisher Scientific) ...
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bioRxiv - Genetics 2021Quote: ... and followed by endogenous HRP quenching before heat-induced epitope retrieval in citrate buffer pH 6 (Target Retrieval Solution, DAKO, cat. number S1700). After epitope retrieval ...
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bioRxiv - Developmental Biology 2020Quote: ... sections were permeabilized with 0.25% TritonX-100 in PBS and autoclaved (121°C, 10 min) with Target Retrieval Solution (Agilent Technologies, Santa Clara, California). Sections were incubated with Blocking One Histo (Nacalai Tesque Inc. ...
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bioRxiv - Developmental Biology 2021Quote: Immunofluorescence microscopy was performed on de-paraffinized tissue sections that received heat-induced antigen retrieval using Target Retrieval Solution (Agilent Dako, S169984-2). After washing and permeabilization in TBS-T universal buffer (0.2% Triton X-100 in tris-buffered saline) ...
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bioRxiv - Plant Biology 2021Quote: ... The absorbance spectra of (de)protonated species in solution were measured from 260 – 800 nm using a Cary 5000 UV-Vis.-NIR spectrophotometer (Agilent, Santa Clara USA) prior to any fluorescence analysis ...
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bioRxiv - Cancer Biology 2020Quote: ... The Illumina TruSeq mRNA libraries with an insert size of about 200 nt were clustered equimolar into a 10nM sequencing stock solution and amplified followed by QC check using 2100 Bioanalyzer (Agilent, Santa Clara, CA). A total of 12 libraries from persistently infected cells and control were generated and sequenced using HiSeq2000 Illumina platform following manufacturer’s instruction (Illumina Inc. ...
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bioRxiv - Developmental Biology 2020Quote: ... IHC followed a routine protocol using a PT link antigen retrieval module and high pH antigen retrieval solution (both Dako Pathology/Agilent Technologies, Stockport, UK). Primary and secondary antibodies were incubated for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Antigen □ retrieval was performed by incubating the tissue for 2 h at 70 °C in Target Retrieval Solution (Dako, Santa Clara, CA, USA) using a 1:10 dilution in demineralized water ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated overnight on a shaker at room temperature in fresh blocking solution with polyclonal rabbit anti-GFAP (1:1000, Dako, Santa Clara, CA). The following day tissue was washed in PBST and incubated for 2.5h at room temperature with Alexa Fluor 488 donkey anti-rabbit secondary (1:500 ...
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bioRxiv - Developmental Biology 2023Quote: ... Twenty embryos were exposed per 2 mL of dosing solution in 2 mL glass amber vials (Cat. No. 5182-0558, Agilent, Santa Clara, CA). Control and dosing solutions were prepared by adding solution constituents to amber vials already containing 20 embryos and gently rocking ...
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bioRxiv - Developmental Biology 2023Quote: ... Twenty embryos were exposed per 2 mL of dosing solution in 2 mL glass amber vials (Cat. No. 5182-0558, Agilent, Santa Clara, CA). Control and dosing solutions were made by adding solution constituents to amber vials already containing 20 embryos and gently rocking ...
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bioRxiv - Neuroscience 2023Quote: ... a solution with the secondary antibody and peroxidase (AdvanceTMHRP®, Dako, cat#K4068, Glostrup, Denmark; or EnvisionTM Flex+, Dako, cat#K8002, Glostrup, Denmark) was added for 30 min at 37°C ...
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bioRxiv - Pathology 2023Quote: ... tissue was subjected to antigen retrieval using a PT link module and high pH antigen retrieval solution (both Dako Pathology/Agilent Technologies, Stockport, UK). For dual immunohistochemistry ...
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bioRxiv - Cell Biology 2023Quote: IHC followed a standard protocol using a PT link antigen retrieval system with high pH antigen retrieval solution (both Dako Pathology/Agilent Technologies, Stockport, UK). Antibody for pSTAT3 (1:100 ...
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bioRxiv - Pathology 2024Quote: ... Antigen epitopes were retrieved with heat induction using EnVision FLEX Target Retrieval Solution (Citrate buffer pH 6.1, Agilent Technologies, Inc., Santa Clara, CA). Tissue autofluorescence was quenched with 0.1% Sudan Black ...
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bioRxiv - Immunology 2024Quote: ... The antigens on the slides were retrieved by submerging slides in 3-in-1 Target Retrieval Solution (pH 9, DAKO Agilent, Cat# S2375) and incubating at 97 °C for 40 min in a Lab Vision PT Module (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: ... The ESI method was then transferred to a MALDI source method and tuned using an Agilent tune mix solution (Agilent Technologies, Santa Clara, CA). For the MALDI MSI conditions ...