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Citations for Agilent :
551 - 600 of 762 citations for Poloxamer 188 solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Frozen sections and paraffin sections were treated with or without heat-based antigen retrieval in Target Retrieval solution (Dako) at 105 °C for 15 min ...
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bioRxiv - Genomics 2022Quote: ... Sample concentration and profiles were determined using Qubit dsDNA HS 1X solution (Thermo Fischer scientific) and D5000 Tapestation (Agilent), respectively.
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bioRxiv - Neuroscience 2022Quote: ... The slides were washed with 0.1 M Sorensen’s phosphate buffer and the staining was developed with a 1:50 solution of DAB chromogen (K3468, Dako) and 0.005% hydrogen peroxide in Sorensen’s phosphate buffer for 20 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... Selected de-waxed brain paraffin sections containing dorsal hippocampus were treated with DakoTarget retrieval solution (pH 9) (Dako, Denmark) at 95° in a Dako PT Link to retrieve protein antigenicity ...
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bioRxiv - Immunology 2024Quote: ... all sections were blocked in a ready-to-use serum free protein blocking solution (Dako/Agilent, Santa Clara, CA) for 10 minutes at room temperature ...
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bioRxiv - Immunology 2024Quote: ... all sections were blocked in a ready-to-use serum free protein blocking solution (Dako/Agilent, Santa Clara, CA) for 10 minutes at room temperature ...
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bioRxiv - Systems Biology 2023Quote: ... The sections were then immersed in epitope retrieval buffer (Target Retrieval Solution, pH 9, DAKO Agilent, Santa Clara, CA) and incubated at 97°C for 40 min and cooled down to 65°C using Lab vision PT module (Thermofisher Scientific ...
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bioRxiv - Systems Biology 2023Quote: ... The sections were then immersed in epitope retrieval buffer (Target Retrieval Solution, pH 9, DAKO Agilent, Santa Clara, CA) and incubated at 97°C for 40 min and cooled down to 65°C using Lab vision PT module (Thermofisher Scientific ...
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bioRxiv - Systems Biology 2023Quote: ... The sections were then immersed in epitope retrieval buffer (Target Retrieval Solution, pH 9, DAKO Agilent, Santa Clara, CA) and incubated at 97°C for 40 min and cooled down to 65°C using Lab vision PT module (Thermofisher Scientific ...
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bioRxiv - Systems Biology 2023Quote: ... The sections were then immersed in epitope retrieval buffer (Target Retrieval Solution, pH 9, DAKO Agilent, Santa Clara, CA) and incubated at 97°C for 40 min and cooled down to 65°C using Lab vision PT module (Thermofisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... free-floating brain slices were incubated in a solution of anti-Iba1 antibody (1:2000; Fuji film-Wako) or anti-GFAP antibody (1:10,000; Agilent-Dako) at 4°C overnight ...
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bioRxiv - Immunology 2022Quote: ... deparaffinised sections were heated in a microwave histoprocessor (HistosPro) with DAKO Target Retrieval solution (citrate buffer pH 6.0 (#S2367, DAKO) or pH 9.0 (#S2367 ...
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bioRxiv - Microbiology 2022Quote: ... Final amplicon solutions were quantified using Qubit BR Assay and sizes confirmed on the Bioanalyzer HS DNA chip (Agilent). Samples were sequenced on the PacBio Sequel II instrument using SMRT cell 8M (QB3 Genomics ...
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bioRxiv - Cancer Biology 2023Quote: ... and the immunohistochemical reaction was developed using 3,30-diaminobenzidine tetrahydrochloride (DAB) as a chromogen (Chromomap DAB, Ventana, Roche or DAB solution, Dako) and nuclei were counterstained with Carazzi’s hematoxylin ...
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bioRxiv - Developmental Biology 2023Quote: ... Antigen retrieval was performed in DAKO PT Link in a DAKO Target Retrieval (Low pH) Solution (DAKO, Cat# S1699) at 98°C for 30 min ...
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bioRxiv - Developmental Biology 2023Quote: ... Antigen retrieval was performed either in 1% citrate buffer or in DAKO antigen retrieval solution (S1699, DAKO Agilent, USA) at 97.5 °C.
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bioRxiv - Developmental Biology 2023Quote: ... Antigen retrieval was performed either in 1% citrate buffer or in DAKO antigen retrieval solution (S1699, DAKO Agilent, USA) at 97.5 °C.
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Dietary potassium and cold acclimation additively increase cold tolerance in Drosophila melanogasterbioRxiv - Physiology 2024Quote: ... and 2.5 mg/L; made by dilution of stock solutions [1000 μg/mL, #5190-8525 and #5190-8503, Agilent]) which allowed us to calculate sample concentrations ...
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bioRxiv - Pathology 2024Quote: ... and the stained cells were mounted on glass slides using Fluorescence-Mounting solution (Dako Omnis, Santa Clara, CA, USA).
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bioRxiv - Bioengineering 2024Quote: ... A 10% solution of mTG in PBS was prepared and filtered through a 0.45 μm syringe filter (Agilent Technologies). The final concentration of mTG was 10 U/g (enzyme/gelatin) ...
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bioRxiv - Immunology 2024Quote: ... the slides were washed in 1× PBS and then exposed to Dako Serum-free Protein Block solution (Agilent, USA) for 45 min at room temperature ...
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bioRxiv - Immunology 2024Quote: ... The sections were then immersed in epitope retrieval buffer (Target Retrieval Solution, pH 9, DAKO Agilent, Santa Clara, CA) and incubated at 97°C for 40 min and cooled down to 65°C using Lab vision PT module (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... The sections were then immersed in epitope retrieval buffer (Target Retrieval Solution, pH 9, DAKO Agilent, Santa Clara, CA) and incubated at 97°C for 40 min and cooled down to 65°C using Lab vision PT module (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... The sections were then immersed in epitope retrieval buffer (Target Retrieval Solution, pH 9, DAKO Agilent, Santa Clara, CA) and incubated at 97°C for 40 min and cooled down to 65°C using Lab vision PT module (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... and antigen retrieval performed at 96°C for one hour using pH9 Dako Target Retrieval Solution (S236784-2, Agilent). Sections were stained for CD31 and either collagen I ...
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bioRxiv - Immunology 2024Quote: ... The sections were then immersed in epitope retrieval buffer (Target Retrieval Solution, pH 9, DAKO Agilent, Santa Clara, CA) and incubated at 97°C for 40 min and cooled down to 65°C using Lab vision PT module (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2024Quote: ... in xylene and deposited with a 12 μm inter-fiber spacing on top of large diameter (2 μm) support fibers prepared from 5 wt% solutions of polystyrene (MW: 15,000,000 g/mol, Agilent Technologies) dissolved in xylene ...
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bioRxiv - Neuroscience 2024Quote: ... The primary antibody solution contains mouse anti-smooth muscle actin (SMA) antibody (1:67, Dako, M0851, Santa Clara, CA) for labeling arteries ...
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bioRxiv - Cancer Biology 2024Quote: Slides were deparaffinized and rehydrated as above and were subjected to HIER in Dako Antigen Retrieval Solution (Agilent S1699) in a pressure cooker (at 120°C ...
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Immune organization in sentinel lymph nodes of melanoma patients is prognostic of distant metastasesbioRxiv - Cancer Biology 2024Quote: ... The sections were then immersed in epitope retrieval buffer (Target Retrieval Solution, pH 9, DAKO Agilent, Santa Clara, CA) and incubated at 97°C for 40 min and cooled down to 65°C using Lab vision PT module (Thermo Fisher Scientific ...
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Immune organization in sentinel lymph nodes of melanoma patients is prognostic of distant metastasesbioRxiv - Cancer Biology 2024Quote: ... The sections were then immersed in epitope retrieval buffer (Target Retrieval Solution, pH 9, DAKO Agilent, Santa Clara, CA) and incubated at 97°C for 40 min and cooled down to 65°C using Lab vision PT module (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... We then performed heat-induced epitope retrieval in a rice steamer with Dako Target Retrieval Solution (DakoCytomation, Santa Clara, CA) at 98°C for 25 min ...
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bioRxiv - Genetics 2021Quote: ... Unstained sections underwent de-paraffinization with xylenes and an ethanol gradient prior to heated antigen retrieval in antigen retrieval solution (Dako). Immunofluorescent staining was then performed as follows.
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bioRxiv - Bioengineering 2020Quote: Absorption and fluorescence spectra of CBD-Twitch and ColBD-Twitch in solution were collected on 8453 diode array spectrophotometer (Agilent) and LS-50B (PerkinElmer ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were washed 3 times 10 min in Tris-Triton Solution and mounted on gelatin-coated slides using Fluorescence Mounting Medium (Dako). Z-stack images (4 optical sections ...
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bioRxiv - Cell Biology 2020Quote: The cells were fixed with 4% paraformaldehyde at 4°C for 5 min and permeabilized with 0.1% Triton X-100 at room temperature for 20 min in the presence of a protein-blocking solution consisting of PBS supplemented with 5% normal goat serum (Agilent Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... The membranes were then washed twice with TBS-T followed by incubation for 1 h at room temperature in immunoreaction enhancer solution 2 (Can Get Signal; TOYOBO) with peroxidase-conjugated antibody against rabbit IgG (Dako) as the secondary antibody ...
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bioRxiv - Cancer Biology 2022Quote: ... FFPE sections were loaded into an Agilent pre-treatment module to be dewaxed and undergo heat induced epitope retrieval (HIER) either using low or high pH target retrieval solution (TRS) (K8005, K8004, Agilent). Sections for αSMA staining underwent antigen retrieval using low pH TRS ...
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bioRxiv - Cell Biology 2022Quote: ... Non-specific antibody binding was blocked by incubation for 30 min with blocking solution containing 5% normal goat serum (NGS, Dako) at room temperature followed by overnight incubation at 4 °C with different primary antibodies listed in Table 1 ...
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bioRxiv - Bioengineering 2022Quote: ... of primary antibody with competitor solution was transferred to array hybridization cassettes (Fig. S5) using a Bravo automated liquid handling platform (Agilent) with a 96-channel head ...
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bioRxiv - Neuroscience 2022Quote: ... Human brain sections were rehydrated and antigen demasking was done using EnVisionTM FLEX Target Retrieval Solution (Agilent Technologies, pH 9.0) under heat ...
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bioRxiv - Biochemistry 2022Quote: ... The APPI-Q-TOF MS instrument was calibrated in the mass range of 50-3000 m/z using an internal calibration standard (low concentration tuning mix solution) supplied by Agilent Technologies ...
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bioRxiv - Bioengineering 2022Quote: Retinal cross-sections after deparaffinization were treated with target retrieval solution at 99 °C for 1 h (pH 6.1; Dako #S1699) and washed with 1x PBS ...
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bioRxiv - Cell Biology 2022Quote: ... Protein immunodetection was performed with primary abs diluted in blocking solution during overnight incubation at 4°C: rabbit polyclonal ab against alpha-1-antitrypsin (A0012, Dako, Agilent) at a 1:500 dilution ...
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bioRxiv - Neuroscience 2020Quote: ... DI rinse and wash buffer prior to a 3-minute incubation with Haematoxylin ready-to-use solution (K8018, Agilent DAKO) and a further DI rinse and wash step ...
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bioRxiv - Neuroscience 2020Quote: ... DI rinse and wash buffer prior to a 3-minute incubation with Haematoxylin ready-to-use solution (K8018, Agilent DAKO) and a further DI rinse and wash step ...
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bioRxiv - Neuroscience 2020Quote: ... prior to target retrieval at 97°C for 30 minutes with EnVision FLEX TRS Low pH target retrieval solution (citrate buffer, pH 6.1; K8005, Agilent DAKO), followed by a wash step with wash buffer (GC807 ...
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bioRxiv - Molecular Biology 2020Quote: ... Sensor plates were calibrated overnight in a CO2-free incubator at 37°C using 1 mL/well of XF calibrant solution (Agilent). After polarization ...
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bioRxiv - Cancer Biology 2021Quote: ... slides were de-waxed and antigen retrieval was performed in a pressure cooker at 125°C for 3 min employing an Antigen Retrieval solution (Dako, pH6 for EZH2 ...
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bioRxiv - Cancer Biology 2020Quote: ... diluted 1:100 or CD3 antibodies (Spring biosciences, Clone SP7, ref. M3070) diluted 1:200 in Antibody diluent solution (DakoCytomation). After several washes in PBS ...