Labshake search
Citations for Agilent :
7201 - 7250 of 9267 citations for Human Fragile X Mental Retardation 1 Neighbor Protein FMR1NB ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... This DNA was then subject to an initial round of PCR amplification with primers oTB535 and oTB536 with the Easy A cloning kit (Agilent), digested with NotI (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... Transfer of the Gateway cassette from these two entry vectors to the pBIFCt-2in1-NN destination vector 57 was performed through LR clonase II kit (Agilent).
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bioRxiv - Biochemistry 2022Quote: ... The quality of total RNA was examined using a fragment analyzer system with an Agilent HS RNA kit (Agilent Technologies). Libraries were prepared using an MGIEasy RNA Directional Library Prep Set (MGI Tech Co. ...
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bioRxiv - Microbiology 2023Quote: ... and pKTP109 (catR, Para::ftsI) by using the QuickChange site-directed mutagenesis kit according to the manufacturer’s instruction (Agilent Technologies). The list of primers used in this study was previously published.
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bioRxiv - Developmental Biology 2023Quote: ... RNA concentration and quality of the RNAseq libraries were assessed using a 2100 Bioanalyzer with the High Sensitivity DNA Kit (Agilent), and libraries were sequenced as single-end 50 bp reads on an Illumina HiSeq 2500.
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bioRxiv - Cell Biology 2023Quote: ... The cells were transfected with either EGFP-AKT2 or EGFP-AKT2+ HA-SIRT5 constructs or left untransfected and after 48 hours were subjected to the Mitostress assay kit (Cat# 103015-100) from Agilent, USA28.
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bioRxiv - Neuroscience 2023Quote: ... RNA quality of the tissue sections (RIN > 7) was confirmed using the Agilent RNA 6000 Nano Kit on the Bioanalyzer 2100 system (Agilent). Visium spatial gene expression slides were processed according to manufacturer instructions (10X Genomics ...
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bioRxiv - Cell Biology 2023Quote: ... vector containing the SARS-CoV-2 HA-ORF3a gene was used in site-directed mutagenesis using a QuikChange II site-directed mutagenesis kit (Agilent) according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... RNA integrity was validated on a 2100 Bioanalyser with the Agilent RNA 6000 Nano kit (Agilent Technologies, Santa Clara, USA). Sequencing libraries were generated with the Illumina TruSeq RNA library Prep kit v2 (StarSEQ ...
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bioRxiv - Cell Biology 2023Quote: ... RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). cDNA was synthesized using SMARTer Ultra Low RNA Kit for Illumina Sequencing (634936 ...
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bioRxiv - Cell Biology 2023Quote: ... Site-directed mutagenesis for Drp1 R456E and Y493A was completed to introduce each mutation into this construct individually using the QuikChange Lightning kit (Agilent) with primers from Integrated DNA Technologies (Coralville ...
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bioRxiv - Biophysics 2023Quote: ... derivative encoding two cysteine residues at positions 366 and 511 [constructed from plasmid pGEMD (-Cys)46 by use of site-directed mutagenesis (QuikChange Site-Directed Mutagenesis Kit; Agilent) to replace codons 366 and 511 by a codon encoding cysteine residue] ...
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bioRxiv - Cell Biology 2023Quote: ... the pK2:Nβ-GFP construct was used as template for site-directed mutagenesis by PCR using the QuickChange Lightning Site-Directed Mutagenesis Kit (Agilent) following the manufacturer’s instructions with the concomitant mutagenic primers for each target base substitution (S2 Table) ...
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bioRxiv - Cell Biology 2023Quote: ... The total RNA quality and quantity were analysis of Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number >7.0 ...
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bioRxiv - Microbiology 2023Quote: ... The mpox sA28 and sH2 expression plasmids were generated from the above vaccinia sA28 and sH2 constructs by site-directed mutagenesis using the QuickChange Lightning Site-Directed Mutagenesis Kit (Agilent). All the vaccinia A28 mutant constructs ...
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bioRxiv - Molecular Biology 2023Quote: ... The small amplicon manufacturer’s instructions were followed for the library kit and completed libraries were quantified and verified on the Tapestation 4200 instrument (Agilent). The completed libraries were diluted to 4 nM ...
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bioRxiv - Microbiology 2023Quote: The substitution of amino acids via SDM was carried out using the QuikChange II Site-Directed Mutagenesis Kit (Agilent Technologies) per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The quality of the amplified and bead-cleaned-up libraries was determined using a 5300 fragment analyzer with the standard sensitivity NGS kit (Agilent). Libraries were then pooled ...
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bioRxiv - Molecular Biology 2023Quote: ... The quantification and quality check of libraries was done using the Bioanalyzer 2100 instrument and the D5000 kit (Agilent Technologies). Libraries were pooled and sequenced on a NovaSeq6000 ...
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bioRxiv - Neuroscience 2023Quote: ... RNA integrity was calculated based on the 28S to 18S rRNA ratio using the RNA 6000 Nano Kit and the Agilent 2100 Bioanalyzer (Agilent Technologies Inc. ...
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bioRxiv - Microbiology 2023Quote: ... The isolation of RNA fragments corresponding to ribosomal footprints of 15 – 45 nt size was verified using the RNA 6000 Nano Kit (Agilent) and the Agilent 2100 Bioanalyzer ...
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bioRxiv - Microbiology 2023Quote: ... A R43A PEXEL mutation in UIS21-70-mNG was generated in this plasmid using a QuikChange Lightning Multi Site Directed Mutagenesis kit (Agilent) and the primer P9 ...
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bioRxiv - Microbiology 2023Quote: ... The quality and size distribution of the libraries were assessed on a Fragment Analyzer using the HS NGS Fragment Kit (Agilent). The pooled samples were then sequenced on a NovaSeq6000 (Illumina ...
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bioRxiv - Microbiology 2023Quote: ... All the H2 mutant constructs were subsequently derived from the pEF6-H2R plasmid using a QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent), with mutation accuracy confirmed by sequencing (Genomics Inc. ...
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bioRxiv - Microbiology 2023Quote: ... All subsequent tH2 mutants expressed in bacteria were derived from pET28a-Smt3-tH2 using a QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... Quality of the final library preparation was analysed on the Bioanalyzer using the High Sensitivity DNA reagents kit and cassettes (5067-4626, Agilent). For all the steps involving purification of library intermediates ...
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bioRxiv - Developmental Biology 2023Quote: ... all libraries were ligated to NEBNext® Multiplex Oligos for Illumina® (Dual Index Primer Set I) and quality control was performed using a 2100 Bioanalyser High Sensitivity DNA Kit 5067-4626 (Agilent) and libraries quantified using a QubitTM Fluorometer following the manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: ... and oxygen consumption rates (OCR, XF Cell Mito Stress Test) were performed with a Seahorse XF24 Extracellular Flux Analyzer and test kits (Agilent Technologies ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Size distribution and degradation were assessed using the Fragment analyzer (AATI) and High Sensitivity Large Fragment 50 kb Analysis Kit (Agilent). Purification steps were performed using AMPure PB beads (PacBio) ...
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bioRxiv - Microbiology 2023Quote: ... Total RNA was extracted using the ZYMO RNA purification kit and RNA integrity was confirmed by the TapeStation system (Agilent). Total RNA was used to generate the sequencing libraries using the KAPA Stranded mRNA Seq kit for the Illumina sequencing platform according to the manufacturer’s protocol (KAPA biosystems) ...
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bioRxiv - Microbiology 2023Quote: ... Extracted DNA was assessed for quantity using the Qubit High Sensitivity dsDNA kit and quality using the Genomic DNA ScreenTape on a TapeStation 4200 (Agilent). Libraries were then prepared for Nanopore sequencing using the PCR-free Rapid DNA sequencing Kit (SQK-RAD004 ...
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bioRxiv - Immunology 2023Quote: ... The total RNA quality and quantity were analyzed by Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number >7.0 ...
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bioRxiv - Cell Biology 2023Quote: ... we created all other Gαi3 mutants featured in this work using a QuikChange® Site-Directed Mutagenesis Kit as per manufacturer’s protocol (Stratagene, USA). A variant that is insensitive to ADP-ribosylation by Pertussis Bordetella toxin (PTX ...
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bioRxiv - Genetics 2023Quote: ... after which DNA fragments in a range of 400-700 bp were excised and extracted using Gel Extraction Kit (A&A Biotechnology) The quality and quantity of the libraries were verified with TapeStation system (Agilent) and Qubit 2.0 fluorometer ...
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bioRxiv - Immunology 2023Quote: ... The variable heavy chain sequence of CH42 was subcloned into a plasmid coded with an IgG1 heavy chain backbone containing the N297A 29 mutation via QuikChange Site Directed Mutagenesis kit (Agilent). Antibodies were expressed through co-transfection of heavy and light chain plasmids in Expi293 HEK cells (Thermo Fisher ...
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bioRxiv - Immunology 2023Quote: ... The quality control of the final library products was performed using the Qubit Fluorometer and the Agilent 2100 bioanalyzer with a High Sensitivity Kit (Agilent). Library products were pooled to a final concentration of 4 nM ...
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bioRxiv - Microbiology 2023Quote: ... The quality and quantity of RNA were determined by capillary electrophoresis using an HS RNA kit and 5200 Fragment Analyzer (Agilent). For single-end RNA sequencing (RNA-seq) ...
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bioRxiv - Immunology 2023Quote: ... Agilent Seahorse XF Cell Mito Stress Test Kit was used to perform Mitostress test in the presence of 10mM glucose (Agilent) with addition of Oligomycin ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA integrity and quantity were assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies). A total amount of 1 μg RNA per sample was used as input material for the libraries preparations ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Sections were washed and incubated with a secondary antibody (Polymer-HRP Anti-rabbit and DAB following kit protocol (DC EnVision + System, HRP; DAKO). Slides were counterstained with hematoxylin ...
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bioRxiv - Neuroscience 2023Quote: ... Generation of GluK2 point mutants was performed by site-directed mutagenesis using the QuickChange Mutagenesis Kit (Stratagene, La Jolla, CA). Forward and reverse primer for creation of mutants were ...
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bioRxiv - Biochemistry 2022Quote: ... The N165A and N234A variants of HDM-SARS2-S-del21-D614G were generated by site-directed PCR mutagenesis using the QuickChange II kit (Agilent), and changes were confirmed by sequencing ...
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bioRxiv - Genetics 2023Quote: ... and the point mutations in the PWWP domain of DNMT3B (W263A, D266A, S270P, K276E and K294E) were introduced using the QuikChange II site-directed mutagenesis kit (Agilent). DNMT3BΔN starts from M200 and was generated by PCR ...
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bioRxiv - Genomics 2023Quote: ... R838W and F867L Ephb4 mutations were introduced by site-directed mutagenesis using a QuikChange II XL Site-Directed Mutagenesis Kit (Agilent) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... Final library concentration was assessed by Qubit dsDNA HS Assay Kit (Thermo-Fischer Scientific) and post library QC was performed using Tapestation High Sensitivity D1000 (Agilent) to ensure that fragment sizes were distributed as expected ...
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bioRxiv - Cancer Biology 2023Quote: Whole genome paired-end sequencing was performed using the Agilent SureSelect v3 library preparation kit (Agilent Technologies, Santa Clara, CA). Paired-end 150 base pair reads were generated using an Illumina HiSeq 2500 sequencing instrument ...
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bioRxiv - Cell Biology 2023Quote: ... and Pst I restriction sites of the multiple cloning site of pEGFP-Mieap were introduced using QuikChange Site-Directed Mutagenesis Kits (Agilent) with primers Mut-F1 ...
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bioRxiv - Cell Biology 2022Quote: Mitochondrial ATP-linked respiration and extra cellular acidification rate was measured by the Seahorse XFe96 Analyzer using the Mito Stress Test kit (Agilent). 4×104 cells were seeded and grown for 24 h n DMEM containing 10 % FBS ...
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bioRxiv - Cell Biology 2023Quote: All TTBK2 mutations were generated by PCR-based site-directed mutagenesis by using QuickChange Lighting Site-Directed-Mutagenesis kit (Agilent) using as a template pENTR/D-TOPO (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... the HA epitope sequence was inserted immediately 3’ to the signal peptide sequence by site directed mutagenesis using the QuickchangeXL site directed Mutagenesis kit (Agilent) to obtain the following intermediate vector ...