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Citations for Agilent :
651 - 700 of 773 citations for SARS Coronavirus Nucleoprotein N Term E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... paraffin-embedded tissue sections were dewaxed and stained according to the H/E standard protocol using a CoverStainer (Dako, Agilent).
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bioRxiv - Developmental Biology 2021Quote: ... and the sections were incubated for 1 h at room temperature with biotinylated IgG anti-mouse secondary antibody which is produced in rabbit (E 0354 -Dako Cyt ...
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bioRxiv - Immunology 2022Quote: ... 50 µL of Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 2% FBS was added to each well of a 96-well E-plate (Agilent) to establish the background reading ...
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bioRxiv - Immunology 2022Quote: ... 50ul of RPMI-1640 was first added to each well of the 96-well E-plates (Agilent, Santa Clara, CA) and was measured as background impedance ...
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bioRxiv - Immunology 2023Quote: ... sorted neutrophils were added to CMT-93 (mouse colonic epithelial cells; ATCC) monolayer on 96-well E-plate (Agilent # 300601010), and changes in TEER were measured every 15 minutes for up to 72hrs as described previously (71 ...
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bioRxiv - Genomics 2023Quote: ... the slide was baked at 60 °C for 60 minutes and then processed through the Dako Progressive H&E protocol (Agilent) and using Dako Harris Hematoxylin ...
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bioRxiv - Immunology 2021Quote: Synthesised antigen constructs were transformed into competent XL-1 Blue Escherichia coli cells (Agilent Technologies, Santa Clara, CA, USA) by electroporation ...
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bioRxiv - Microbiology 2021Quote: ... The myr(–)MA and myr(–)MA99 proteins were overexpressed in Escherichia coli BL21 Codon Plus-RIL cells (Agilent Technologies). Cells were grown in LB broth supplemented with 100 mg/L Ampicillin at 37 °C ...
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bioRxiv - Genomics 2021Quote: ... coli were generated by introducing the corresponding mutations in plasmid pDL893 using the QuikChange site-directed mutagenesis kit (Stratagene).
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bioRxiv - Biophysics 2023Quote: Human wild-type α-synuclein and A90C cysteine variants were purified from Escherichia coli BL21 (DE3)-gold (Agilent Technologies) expressing plasmid pT7-7 encoding for α-synuclein as previously described35,42,43 ...
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bioRxiv - Microbiology 2019Quote: ... Hybridized arrays were scanned at 5 μm resolution on a Microarray Scanner (Agilent p/n G2565BA). Data extraction from images was done by using Agilent Feature Extraction (FE ...
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bioRxiv - Immunology 2020Quote: ... and isotype control: Glycans were prepared using the GlykoPrep® Rapid N-Glycan Preparation kit (PROzyme) and separated by Hydrophilic-Interaction Liquid Chromatography (HILIC ...
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bioRxiv - Cancer Biology 2023Quote: ... N-Universal Negative Control Anti-Rabbit or Anti-Mouse (IS600 and IS750, respectively, Dako, Glostrup, Denmark) was used ...
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bioRxiv - Microbiology 2019Quote: ... The RNase E recognition site mutant fusions cfa’-’lacZ-LongG1 and G2 were made by mutating pCB1 using QuikChange mutagenesis (Agilent Technologies) and oligos that restore the G101C mutation to the WT and add the additional desired mutations ...
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bioRxiv - Cancer Biology 2020Quote: ... gels were fixed in formalin prior to paraffin-embedding and staining with haematoxylin and eosin (H+E) and immunohistochemistry (IHC) for p53 (Dako IS616). Stained sections were imaged with a Pannoramic 250 scanner (3DHISTECH Ltd ...
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bioRxiv - Molecular Biology 2019Quote: ... We generated E-box point mutations (CANNTG ➔ CANNTA) with the QuikChange II site-directed mutagenesis kit (Agilent, 200523, Santa Clara, CA) per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... All the mutants used in these cellular assays were generated by using QuikChange II-E Site-Directed Mutagenesis Kit (#200555, Agilent Technologies).
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bioRxiv - Cell Biology 2021Quote: ... CENP-A/H4 was expressed from a pST39 vector in BL21 (DE3) Codon Plus RIPL Escherichia coli (Agilent Technologies, 230280), grown in 2xYT medium (20 g/liter tryptone ...
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bioRxiv - Plant Biology 2022Quote: His6-MBP-PFA-DSP protein fusions or free His8-MBP were expressed in Escherichia coli BL21 CodonPlus (DE3)-RIL cells (Stratagene). Overnight bacterial cultures were inoculated 1:1000 into fresh 2YT medium (1.6 % tryptone ...
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bioRxiv - Developmental Biology 2022Quote: ... The pETM11-Sumo3-Tn5E54K,L372P plasmid was obtained from EMBL-Heidelberg and transformed into Escherichia coli BL21 (DE3) Gold cells (Agilent). Bacterial cultures were grown at 30◦C to a density of OD600=0.6-0.8 and protein expression was induced with 0.5 mM isopropyl-β-D-thiogalactoside (IPTG) ...
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bioRxiv - Genomics 2021Quote: The different Sen1 HD versions were fused to a C-terminal His8-tag coupled to Vibrio cholerae MARTX toxin cysteine protease domain (61)and expressed from the T7 promoter (see plasmids in table S6) in Escherichia coli strain BL21 (DE3) CodonPlus (Stratagene). Overexpression was induced by growth in auto-inducing medium (62 ...
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bioRxiv - Cell Biology 2020Quote: ... including glutathione S-transferase (GST)-NEPH1 cytoplasmic domain (CD) and GST-NEPHRIN CD were expressed and purified from Escherichia coli BL21 cells (Stratagene). Purified phosphorylated GST-NEPH1 was expressed and purified from TKB1 cells (Stratagene) ...
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bioRxiv - Biophysics 2020Quote: His-tagged CrSAS-6[NL] spanning amino acids 1-503 of the protein (see Supplementary Fig. 1a)1 was expressed in the Escherichia coli strain BL21(DE3) (Stratagene). Bacteria were grown at 37 °C in lysogeny broth (LB ...
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bioRxiv - Biophysics 2020Quote: A Pol I expression vector was generated from the plasmid pXS67 (Yale Coli Genetic Stock Center, Strain CJ803) by site-directed mutagenesis using a QuikChange kit (Agilent) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... We produced the purified 16S V4 rDNA by amplification of the V4 region of Escherichia coli XL 10-gold (Agilent) using TaKaRa Taq R001 AM kit (Clonetech ...
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bioRxiv - Microbiology 2022Quote: ... The obtained amplicons were blunt-cloned into an EcoRV-digested pBluscript KS+ plasmid and transformed into the Sure Escherichia coli strain (Stratagene), cultured at 30 °C.
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bioRxiv - Plant Biology 2022Quote: Plasmids were transformed and expressed in the Escherichia coli strain BL-21 (DE3) RIL cells (Agilent, Santa Clara, CA, USA). For protein expressions ...
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bioRxiv - Biochemistry 2023Quote: The pET-30a-PHB1 vector encoding His-tagged mouse PHB1 was transformed into Escherichia coli strain BL21 Star (DE3; Stratagene). The resulting N-terminal His-tagged recombinant PHB1 was purified using Ni-Sepharose 6 Fast Flow (GE Healthcare) ...
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bioRxiv - Biophysics 2023Quote: Human MEK1 kinase domain (residues 1-393 with a TEV protease cleavable N-terminal 6x-His tag in pET-Duet) containing an inactivating K97R mutation was expressed in chemically competent BL21 (DE3) RIL Escherichia coli (Agilent) for 18 h at 18°C ...
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bioRxiv - Biophysics 2023Quote: Human BRAF kinase domain (residues 448-723 with a tobacco etch virus (TEV)-protease-cleavable N-terminal 6x-His tag in pProEX containing 16 mutations to improve solubility38 was expressed in chemically competent BL21 (DE3) RIL Escherichia coli (Agilent) for 18 h at 18°C ...
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bioRxiv - Cancer Biology 2020Quote: ... was fragmented to 200bp using a Covaris E Series and the resultant libraries were subjected to DNA Capture using SureSelect XT Mouse All Exon kit (Agilent, Stockport, Cheshire) following manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... TMEM24(ΔBS + 5S → E)-eGFP was generated using site-directed mutagenesis to remove a portion of the C-terminus of TMEM24(5S → E)-eGFP including the first 3 β-strands of the β-sheet band 4.1 interacting domain (Quik-Change II XL; Agilent Technologies).TMEM24(5S → E)-eGFP which has been previously described (Sun et al. ...
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bioRxiv - Bioengineering 2023Quote: ... bEnd.3 cells were seeded at a density of 6×103 cells/well in rat tail collagen-coated (150 µg/mL) 96-well plates (E-plate 96; Agilent, cat# 300600910) and confluent monolayers were treated as described above for human ECs ...
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bioRxiv - Bioengineering 2023Quote: ... label-free measurement of barrier integrity was performed in 96-well plates with integrated gold electrodes (E-plate 96; Agilent, cat# 300600910) using an xCELLigence RTCA SP device (Agilent) ...
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bioRxiv - Bioengineering 2023Quote: ... rat ECs were seeded at a density of 6×103 cells/well in 96-well plates (E-plate 96; Agilent, cat# 300600910) coated with collagen IV (100 μg/mL ...
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bioRxiv - Biochemistry 2020Quote: Skd3 variants were expressed as an N-terminally MBP-tagged protein in BL21 (DE3) RIL cells (Agilent). Cells were lysed via sonication in 40mM HEPES-KOH pH=7.4 ...
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bioRxiv - Biochemistry 2022Quote: ... PARLSkd3 and variants were expressed with an N-terminal MBP-tag in BL21 (DE3) RIL cells (Agilent). Cells were lysed via sonication in lysis buffer (40 mM HEPES-KOH pH = 7.4 ...
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bioRxiv - Microbiology 2020Quote: ... and n-caproate concentrations from the pH experiment were analyzed with an Agilent 7890B Gas Chromatograph (Agilent Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... Japan) 41 with 2x hemagglutinin (HA) epitopes on the N-terminus into the pAAV-MCS vector (Agilent) and AAV9 was produced by Vigene or the Howard Hughes Medical Institute Viral Vector Core ...
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bioRxiv - Cell Biology 2020Quote: ... and Pnut with a C-terminal Strep tag II (WSHPQFEK, 1058 Da) were purified from Escherichia coli BL21(DE3) cells (Agilent Technologies) using a two-tag affinity purification scheme to aid the isolation of full-length complexes as explained in ref ...
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bioRxiv - Cell Biology 2020Quote: Zyxin and Pxl1 LCR proteins were expressed in Escherichia coli strain BL21-Codon Plus (DE3)-RP (Agilent Technologies, Santa Clara, CA) with 0.5 mM isopropyl β-D-1-thiogalactopyranoside for 16 h at 16 °C ...
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bioRxiv - Evolutionary Biology 2022Quote: A pET-24B plasmid containing Marseillevirus marseillevirus doublet histones (Hδ-Hγ with a C-terminal 6×His tag and Hβ-Hα) (Valencia-Sanchez et al., 2021) was transformed into Escherichia coli BL2-codon plus(DE3)-RIL competent cells (Agilent Technologies) and cultured at 37°C in 2xYT-Kanamycin medium ...
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bioRxiv - Microbiology 2022Quote: ... coli and used as a template for generation of E2 mutants by a QuikChange Lightning Site Directed Mutagenesis Kit (Agilent Technology) following the supplier’s procedure ...
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bioRxiv - Cell Biology 2023Quote: ... and GST-SDPN-1 SH3 (P479L) fusion proteins were expressed in the ArcticExpress strain of Escherichia coli (Stratagene, La Jolla, CA). Bacterial pellets were lysed in 10 ml B-PER Bacterial Protein Extraction Reagent (Pierce ...
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bioRxiv - Physiology 2023Quote: ... to alanine(s) (A) or glutamate(s) (E) by site-directed mutagenesis using the QuikChange II XL Site-Directed Mutagenesis kit (Agilent, Santa Clara, CA) of a pDUAL2-CCM(- ...
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bioRxiv - Molecular Biology 2021Quote: PFDN5 ORF (splice variant alpha) was cloned EcoRI/SalI into pCMV-Tag 2A (N- terminal Flag tag, Agilent) or XhoI/EcoRI into pEGFP-C1 (N-terminal GFP tag ...
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bioRxiv - Biochemistry 2020Quote: ... followed by horseradish peroxidase (HRP)-conjugated anti-mouse antibodies (Cat. N. P044701-2, Agilent Technologies LTD, Cheadle, UK) (2.4μg/mL ...
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bioRxiv - Microbiology 2019Quote: ... Amplification of the HMPV N gene was performed by quantitative PCR using SYBR Green qPCR Master Mix (Agilent) and a set of primers listed in Table 1 ...
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bioRxiv - Biochemistry 2021Quote: The second set of experiments was performed on Seahorse XF Cell Culture Microplates (Agilent, cat. n. 103022-100) with eight wells ...
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bioRxiv - Microbiology 2024Quote: ... CH4 samples were analyzed immediately by flame ionization detection gas chromatography (Agilent 6890 N with Porapak Q column). The wetlands that were sampled in both experiments have no known prior history of agricultural use but are affected by runoff from surrounding agricultural and urban areas ...