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Citations for Agilent :
651 - 700 of 2406 citations for 5 2 3 Difluorophenyl 5 oxovaleric acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... The samples were then transferred immediately into a 5 mm NMR tube (Wilmad) and placed into a 600 MHz magnet with a coldprobe (Agilent). The peptide backbone was assigned using a combination of BEST versions of 3D HNCA ...
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bioRxiv - Microbiology 2023Quote: ... The optical density was measured at 540 nm and subtracted from readings at 450 nm using the BioTek Cytation 5 Cell Imaging Multimode Reader (Agilent). Concentrations were calculated from standard curves run in parallel.
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bioRxiv - Systems Biology 2023Quote: ... the peptides were reconstituted in 10 mM TEAB and fractionated using a bRPLC column (Agilent 300 Extend-C18 column, 5 µm, 4.6 mm × 250 mm, Agilent Technologies) under an increasing gradient of the mobile phases consisting of 10 mM TEAB in water and 90% acetonitrile (ACN) ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were washed in PBS and incubated in DAPI (1:500) for 5 min before mounting with the immunofluorescence mounting media (DAKO).
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bioRxiv - Microbiology 2023Quote: ... Membrane fluidity was determined by using an excitation wavelength of 350 nm and measuring fluorescence at 460 nm and 500 nm emission wavelengths on a BioTek Cytation 5 Cell Imaging Multimode Reader (Agilent). The generalised polarisation (GP ...
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bioRxiv - Microbiology 2023Quote: ... 200 μl were aliquoted into black 96 well plates in triplicate and fluorescence was measured using a BioTek Cytation 5 Cell Imaging Multimode Reader (Agilent). The excitation wavelength used was 488 nm and emission wavelength used was 530 nm.
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bioRxiv - Bioengineering 2023Quote: ... slides were counterstained with DAPI (1µg/ml) in PBS for 5 min followed by water washes and cover slipping with fluorescent antifade mounting reagent (DAKO, Agilent).
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bioRxiv - Bioengineering 2023Quote: ... Peptides were loaded to a nanoscale HPLC column composed of 10 cm of Polaris C18 5 μm packing material (Agilent), followed by 4 cm of Partisphere 5 SCX (Whatman) ...
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bioRxiv - Cell Biology 2024Quote: ... Mixtures were incubated at RT for 60 min in the dark and absorbance at OD570 was measured on a microplate reader (Bio Tek Cytation 5, Agilent). For each LD sample ...
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bioRxiv - Immunology 2024Quote: ... Sections were blocked with 5% horse serum in 1% BSA solution and incubated with the anti-CD68 primary antibody (KP1, Dako) for 60 minutes at room temperature ...
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bioRxiv - Genomics 2024Quote: ... each library underwent a reconditioning PCR to reduce heteroduplexes: libraries were concentrated down to 5 µl and mixed with 10 µl of Herculase Buffer (Agilent), 5 µl of 2.5 nM dNTPs ...
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bioRxiv - Plant Biology 2024Quote: ... The plates were placed in either a BioTek Cytation 5 or Synergy HT plate reader (Agilent, Santa Clara, CA, USA) set at 22°C ...
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bioRxiv - Cell Biology 2024Quote: ... The HPLC conditions employed two normal-phase Zorbax Sil (5 μm, 4.6 × 150 mm) columns (Agilent, Santa Clara, CA, USA), connected in series within the Multicolumn Thermostat compartment ...
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LRBA balances antigen presentation and T-cell responses via autophagy by binding to PIK3R4 and FYCO1bioRxiv - Immunology 2024Quote: ... cell nuclei were stained with DAPI for 5 min prior to mounting the cover slip onto a glass slide using DAKO mounting medium (Agilent). Cells were visualized through 63x ...
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bioRxiv - Cancer Biology 2024Quote: ... 2,000 cells were seeded on a well of a 96-well plate and brightfield images were taken every two hours by using the BioTek BioSpa 8 Automated Incubator and Citation 5 reader (Agilent). BioTek Gen5 imaging software (Agilent ...
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bioRxiv - Immunology 2024Quote: ... We performed the HPLC with a normal phase Zobax Sil (5 μm, 4.6 × 150 mm) column (Agilent, Santa Clara, CA). Isocratic chromatographic separation was achieved with 10% ethyl acetate/hexane at a flow rate of 1.4 ml/min ...
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bioRxiv - Microbiology 2024Quote: The archived samples of DNA extracts were tested for endogenous KoRV-5’ using an AriaMx real-time PCR system (Agilent) with recKoRV_200 primers under the optimised conditions (see results) ...
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bioRxiv - Physiology 2024Quote: ... interfaced to a nanoscale reversed-phase high-pressure liquid chromatography (Tempo) with a 10 cm-180 micron ID glass capillary packed with 5-µm C-18 ZorbaxTM beads (Agilent) as described before 37.
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bioRxiv - Biochemistry 2024Quote: ... A Zorbax SB-C8 reversed-phase column (5 μm, 2.1 x 50 mm) was obtained from Agilent (Palo Alto, CA). The LC eluent was introduced into the ESI source of the mass spectrometer ...
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bioRxiv - Neuroscience 2024Quote: ... sound stimuli consisted in single-cycle sound pips (5-ms duration at 200 Hz) produced by a function generator (33210A Agilent Technologies Inc. ...
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Downregulation of Let-7 miRNA promotes Tc17 differentiation and emphysema via de-repression of RORγtbioRxiv - Immunology 2024Quote: ... This construct was also used to generate the let-7 ʻseed’ deletion mutant derivative using the QuikChange Multi Site Mutagenesis Kit (catalog 200514-5, Stratagene). 3T3 mouse embryonic fibroblasts (MEFs ...
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bioRxiv - Molecular Biology 2024Quote: ... equipped with a DB-5 column (inner diameter, 0.25 mm; length, 30 m; film thickness, 1 μm; #122-5033, Agilent Technologies). Helium was used as carrier gas ...
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bioRxiv - Molecular Biology 2024Quote: ... with the shRNA target site (5’-TGGAGAACTTGGAAATGGAA) in PCNA disrupted by site-directed mutagenesis using the Quick Change system (Stratagene). Stable cell lines expressing HA-PCNA-WT or HA-PCNA-K164R were generated by lentiviral infection ...
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bioRxiv - Plant Biology 2024Quote: ... and 1.5 μl of this dilution was added to 5 μl of qRT-PCR Brilliant III SYBR Master Mix (Agilent Technologies). Quantitative Real-time PCR (qRT-PCR ...
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bioRxiv - Biophysics 2024Quote: ... a ∼5 V cm-1 electric field was manually applied across the compartment using a DC power supply (E3620A, Agilent) to pull the chromosome into the bottom capillary ...
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bioRxiv - Biochemistry 2024Quote: Analytical SEC was conducted on a Nexera Bio HPLC (Shimadzu) using a Bio SEC-5 2000 Å pore size column (7.8 × 300 mm, Agilent) with a Bio SEC-5 2000 Å guard column (7.8 × 50 mm ...
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bioRxiv - Cell Biology 2024Quote: ... FITC-dextran levels were measured (ex: 485 nm/em: 528nm) based on a standard curve on an Biotek Cytation 5 (Agilent) microplate reader ...
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bioRxiv - Cancer Biology 2024Quote: ... and imaged with the 4x objective every 12 h for 10-14 days using a BioTek Cytation 5 Cell Imaging Multimode Reader (Agilent). Images were processed for data acquisition using BioTek GenV software (Agilent) ...
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bioRxiv - Cell Biology 2024Quote: ... Luciferin Detection reagent was added to all wells and luciferase readings were taken using a BioTek Cytation 5 (Agilent Technologies) plate reader.
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bioRxiv - Cancer Biology 2024Quote: ... with 0.1% v/v Ammonium Hydroxide (Alfa Aesor) in 90% Water (Honeywell)/10% Acetonitrile (BioSolv) containing 5 µM Infinitylabs Deactivator Solution (Agilent). Solvent B was 90% Acetonitrile / 10% Water containing 5 µM Infinitylabs Deactivator Solution ...
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Dual MAPK and HDAC inhibition rewires the apoptotic rheostat to trigger colorectal cancer cell deathbioRxiv - Cancer Biology 2021Quote: ... Chromagen was developed using the DAB (3, 3-diaminobenzidine) reagent (Dako). Sections were counter-stained using pre-filtered Mayer’s haematoxylin (Amber Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Antigen–antibody complexes were revealed with 3-3’-diaminobenzidine (K3468, Dako). Sections were counterstained with haematoxylin (Dako ...
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bioRxiv - Cell Biology 2020Quote: ... Antigen-antibody complexes were revealed with 3-3’-diaminobenzidine (K3468, Dako). Sections were counterstained with hematoxylin (Dako ...
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bioRxiv - Physiology 2022Quote: ... Antigen–antibody complexes were revealed with 3-3′- diaminobenzidine (K346811, Dako) or the DAB (Polymer ...
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bioRxiv - Cell Biology 2024Quote: ... at 1:500 and 3-3’-diamino-benzidine-tetrahydrochloride (Dako, K3468). Stained sections were imaged using an NanoZoomer 2.0HT (Hamamatsu).
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bioRxiv - Cancer Biology 2024Quote: ... Antigen–antibody complexes were reveled with 3-3′-diaminobenzidine (K346811, Agilent). Sections were counterstained with hematoxylin (CS700 ...
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bioRxiv - Physiology 2023Quote: ... Hepatic portal vein bile acids and short chain fatty acids were acquired using an Agilent 1290 series HPLC (Agilent,) with an Acquity C18 BEH (2.1mm x 50mm ...
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bioRxiv - Neuroscience 2023Quote: ... samples were centrifuged at 20.800 xg for 2 min and 100 µl sample was injected on a SEC-HPLC column (Bio SEC-3 300 Å, Agilent, USA) using an Agilent 1260 Infinity II system (Agilent ...
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bioRxiv - Bioengineering 2021Quote: ... 10-20 µl injection volume depending on protein concentration) were injected on a Poroshell 300SB-C8 column (5 µm, 300Å, 1×75mm IDxL; Agilent Technologies) at a flow rate of 100 µl/min using solvent A (0.1% formic acid and 0.05% trifluoroacetic acid in water ...
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bioRxiv - Molecular Biology 2021Quote: Cross-linked peptides were enriched with Fe(III)-NTA 5 µL in an automated fashion using the AssayMAP Bravo Platform (Agilent Technologies). Fe(III)-NTA cartridges were primed with 250 µL of 0.1% TFA in ACN and equilibrated with 250 µL of loading buffer (80% ACN/0.1% TFA) ...
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bioRxiv - Genomics 2020Quote: ... gDNA before SRE treatment was diluted 5-fold and the undiluted solution after SRE treatment was used according to the protocol using TapeStation 2200 (Agilent Technologie) and Genomic DNA ScreenTape (Agilent Technologie).
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bioRxiv - Plant Biology 2020Quote: ... All samples were diluted to 3.5 % HNO3 prior to multi-elemental analysis using Inductively-Coupled Plasma Optical Emission Spectrometry (ICP-OES) (5100 ICP-OES, Agilent Technologies). The ICP-OES was equipped with a SeaSpray nebuliser and a double pass scott type spray chamber ...
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bioRxiv - Systems Biology 2020Quote: ... 3 μl of each sample containing approximately 10 μg of total native peptides and 500 fmol of each stable isotope-labeled standard (SIS) peptide were loaded on an analytical column, Zorbax 300SB-C18 (5 μm, 150 × 0.3 mm) (Agilent Technologies, USA) and washed with 5% acetonitrile for 5 min at a flow rate of 20 μl/min ...
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bioRxiv - Cancer Biology 2021Quote: ... The DNA was denatured at 95°C for 5 min in a PCR machine (PTC-200, MJ Research; PCR strip tubes (Agilent 410022)) and then incubated with the biotin capture RNA at 65°C ...
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bioRxiv - Biochemistry 2020Quote: The peptide was purified by high-performance liquid chromatography (HPLC) on a C4 column (Phenomenex Jupiter C4, 5 μm, 300 Å, 250 × 10 mm, Agilent) using an acetonitrile/water gradient containing 0.1% TFA ...
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bioRxiv - Systems Biology 2021Quote: ... using an Aminex HPX-87H ion-exchange column operated at 60°C with 5 mM H2SO4 as the mobile phase with a flow rate of 0.6 mL min-1 (Agilent, Santa Clara). The OD660 was measured with a Jenway 7200 spectrophotometer (Jenway ...
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bioRxiv - Microbiology 2021Quote: ... Online mass calibration was performed using a second spray needle and a constant flow (5 ul/min) of reference solution containing purine and hexakis (1H, 1H, 3H -tetrafluoropropoxy)phosphazine (HP-0921, Agilent Technologies). Compounds were identified based on the retention time of chemical standards and their accurate mass (tolerance 20 ppm) ...
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bioRxiv - Microbiology 2021Quote: ... Online mass calibration was performed using a second spray needle and a constant flow (5 ul/min) of reference solution containing purine and hexakis (1H, 1H, 3H -tetrafluoropropoxy)phosphazine (HP-0921, Agilent Technologies). Compounds were identified based on the retention time of chemical standards and their accurate mass (tolerance 20 ppm) ...
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bioRxiv - Plant Biology 2021Quote: ... and 0.25 μm film of 95% dimethyl/5% diphenylpolysiloxane) with a precolumn (10 m J&W integrated with Agilent 122-5532G) was used for compound separation ...
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bioRxiv - Cell Biology 2021Quote: ... The reaction was quenched by addition of glycerol (5% final) and desalted using a Bond Elut SepPak C18 cartridge (Agilent, MA). Methanol was used to elute oxidized GM1 species from the column and was removed by Speed Vac concentration (Savant) ...