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Citations for Agilent :
551 - 600 of 2406 citations for 5 2 3 Difluorophenyl 5 oxovaleric acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... The purity of LCA probe was determined to be >95% by HPLC with UV detection at 220 nm (HD-C18 column, 5 μm, 4.6 × 250 mm, Agilent Technologies ...
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bioRxiv - Biochemistry 2023Quote: ... Gly241Glu and Asp235Ala:Gly241Glu were introduced into the previously published vectors (5) using the QuickChange II Site-directed mutagenesis kit (Agilent) using primers listed in Table S2.
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bioRxiv - Biochemistry 2023Quote: ... Colonies were stained with Wright-Giesma and plates were scanned using the Biotek Cytation 5 Imaging Multimodal Reader (Agilent). From scanned images ...
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bioRxiv - Synthetic Biology 2023Quote: ... fitted with a guard column (Zorbax Eclipse XDB-C18, 2.1 x 5 mm 1.8 μm, Agilent part # 821725-903) using a 1290 Infinity II UHPLC (G7120AR ...
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bioRxiv - Bioengineering 2024Quote: ... Primary Cells were stained with DAPI and then imaged with a high throughput imaging system (BioTek Cytation 5, Agilent).
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bioRxiv - Cancer Biology 2023Quote: ... used a two solvent gradient (Mobile Phase A: 10 mM Ammonium Acetate in 90/10 Water/Acetonitrile, pH 9.2, 5 μm Agilent InfinityLab Deactivator Additive ...
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bioRxiv - Plant Biology 2024Quote: ... The organic phase was dried using anhydrous MgSO4 and analysed on GC-2010 Pro gas chromatograph (Shimadzu, Japan) equipped with an HP-5MS 30 m × 0.25 mm (5%-Phenyl)-methylpolysiloxane column (19091S-133, Agilent) with nitrogen as the carrier gas with splitless injection mode ...
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bioRxiv - Immunology 2022Quote: ... The HPLC system consisted of a Thermo Scientific Ultimate 3000 equipped with a UV detector set at 274 nm and a reverse phase Zorbax 300SB-C18 column (4.6 × 250 mm i.d., pore size 5 μm Agilent, U.S.A.). The mobile phase consisted of a 50/50 acetonitrile/buffer (0,02 % ammonium acetate solution at pH 5 ...
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bioRxiv - Cell Biology 2022Quote: ... In brief, peptides were loaded on a Zorbax 300-Extend-C-18 (5 µm, 4.6 × 250 mm) column (Agilent) at a constant flow rate of 1 mL/min ...
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bioRxiv - Microbiology 2022Quote: ... Microarray images were collected by using sure scan microarray scanner system at 5 μm resolution (Agilent Technologies, Scanner 2600D). Fluorescent intensities were quantified using feature extraction software ...
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bioRxiv - Plant Biology 2022Quote: ... and 1 mL injections (passed through silica to filter out precipitate following derivatization) on a Zorbax RX-silica (250 x 4.6 mm, 5 µm; Agilent) column and a mobile phase consisting of solvent A (100% hexanes ...
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bioRxiv - Genomics 2022Quote: ... The GC was equipped with a 30 m DB-35MS + 5 m DuraGuard capillary column (0.25 mm inner diameter, 0.25 μm film thickness; Agilent Technologies). Helium was used as carrier gas at a flow rate of 1.0 mL min−1 and the GC-oven was run at following temperatures and times per sample ...
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bioRxiv - Microbiology 2023Quote: ... of the in vitro SAM cleavage reaction were separated by reverse-phase high performance liquid chromatography (HPLC) with Zorbax Eclipse Plus C18 5-μm (4.6 by 250 mm) column (Agilent) connected to the 1220 Infinity II LC system (Agilent) ...
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bioRxiv - Microbiology 2023Quote: ... Using a LC-10AT HPLC instrument (Shimadzu), the pooled sample was loaded onto a ZORBAX Eclipse XDB C18 column (5 μm, 4.6 × 150 mm) (Agilent) that had been equilibrated with 25 mM sodium acetate ...
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bioRxiv - Cancer Biology 2023Quote: ... for 5 minutes and incubated with the primary antibody of interest which was prepared in antibody diluent (S0809, Dako). Slides were put in a humidified chamber at 4C overnight ...
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bioRxiv - Plant Biology 2024Quote: ... using a HP-5MSI 5% phenyl methyl silox with 30 m × 250 µM × 0.25 µm film thickness (Agilent Technologies). Identification and quantification were conducted using AMDIS (Automated Mass spectral Deconvolution and Identification System ...
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bioRxiv - Physiology 2024Quote: ... and injected 100 µL of sample into an Agilent 1100 series HPLC fitted with a Zorbax RX-SIL column (4.6x25 mm, 5 µm, Agilent). Elution of samples was achieved by a gradient mobile phase of 0.5% ethyl acetate in hexane for 5 minutes then increased to 10% ethyl acetate in hexane for 20 minutes ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... As species were separated by ion-pairing chromatography using a Zorbax SB-C18 column (150 mm × 4.6 mm, 5 μm particle size, Agilent) through isocratic elution for 25 min with the mobile phase used for extraction ...
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bioRxiv - Developmental Biology 2024Quote: ... The material was loaded on to a Zorbax 2.1 mm x 150 mm (5 µm particle size) Extend-C18 column (Agilent) for basic reverse-phase fractionation ...
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bioRxiv - Developmental Biology 2024Quote: ... Point mutations were introduced into sel-5 and dpy-23 sequences using the QuikChange Site-directed Mutagenesis kit (Agilent). Transcriptional reporter Ppgp-12::gfp was created by inserting 3410 bp of pgp-12 promoter upstream of GFP in pPD95.81 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then rinsed with PBS containing 1% FBS prior to imaging using a Cytation 5 instrument (Agilent BioTek). Mean fluorescence was calculated using Gen5 software (RRID:SCR_017317).
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bioRxiv - Physiology 2024Quote: ... Secreted protein in cell culture media was enriched using StrataClean Resin (Agilent, 10 µl per 5 ml of media) as previously described37 ...
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bioRxiv - Cancer Biology 2024Quote: ... The eluted peptides were then trapped in a reverse-phase column (Zorbax 300SB-C18, 0.3 × 5 mm; Agilent Technologies), and separated on a homemade column (HydroRP 2.5 µm ...
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bioRxiv - Microbiology 2024Quote: ... reverse-phase LC was performed on a Zorbax Eclipse XDB-C8 column (150 mm, 4.6 mm, 5 µm; Agilent) using Shimadzu UFLC XR instrument ...
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bioRxiv - Microbiology 2024Quote: ... Incubation was carried out at 30 °C in a plate reader (BioTek Cytation 5; Agilent, Santa Clara, CA, USA) and the data were recorded using Biotek Gen5 software (Agilent) ...
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bioRxiv - Systems Biology 2024Quote: ... were directly injected using a Shimazu LC-40 UHPLC onto a 5 cm x 2.1 mm C18 column analytical column (Agilent InfinityLab Poroshell 120 EC-C18 ...
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bioRxiv - Genomics 2024Quote: ... Each protein sample was injected onto an Poroshell 300SB-C8 column (2.1 x 75 mm, 5 µM, room temp, Agilent) and separated using a linear gradient from 5% acetonitrile for 0 to 2 min and ramping to 95% acetonitrile over 7.5 min ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were Hoechst-stained and relative cell numbers counted using a BioTek Cytation 5 Cell Imaging Multimode Reader (Agilent) at the specific timepoints.
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bioRxiv - Developmental Biology 2024Quote: ... Mutations in the miRNA binding sites were introduced using the QuikChange II XL system (Agilent Technologies; CatNo. 200521-5). Primers used are shown in Table S2 ...
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bioRxiv - Microbiology 2024Quote: ... cells were incubated for 30 minutes in 5 mM MβCD in fresh XFp medium (Agilent Technologies, Santa Clara, CA). To interrogate mitochondrial function ...
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Co-Metabolic Growth and Microbial Diversity: Keys for the depletion of the α, δ, β and γ-HCH isomersbioRxiv - Microbiology 2024Quote: ... internally coated with a 0.18 µm film composed of a non-polar stationary phase containing 5% diphenyl-polysiloxane (HP5-MS, Agilent). The injection was performed in split-less mode at 280°C ...
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bioRxiv - Microbiology 2024Quote: Content of the rRNA hydrolysate was resolved using Zorbax SB-C18 column (5 µm, 0.5 x 150 mm, Agilent) and the LC conditions were tuned empirically ...
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bioRxiv - Cancer Biology 2024Quote: ... Specified gradients for HPLC purifications (MeCN in 0.2% TFA (aq.)) were linear (5 mL/min for 12 min (Agilent) or 25 mL/min for 10 min (Waters)) ...
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bioRxiv - Biochemistry 2024Quote: ... The fluorescence of samples was measured at 25 °C using Cytation 5 Cell Imaging Multimode Reader (Agilent Technologies, USA). The mean fluorescence intensities (in arbitrary units ...
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bioRxiv - Immunology 2024Quote: ... and 2-3 x 105 NK cells per well were seeded in triplicates in Seahorse XF RPMI medium (Agilent, 103576-100) supplemented with 2 mM L-glutamine (Agilent ...
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bioRxiv - Biochemistry 2024Quote: ... These samples were desalted using preequilibrated (3x 15 µL of 50% ACN 0.2% Formic acid then 3X 15 µL 0.2% formic acid) Cleanup C18 pipette tips (Agilent) by pipetting 15 µL of the acidified solution 10X to ensure full binding of the peptides to the C18 plug in the tips ...
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bioRxiv - Molecular Biology 2020Quote: - Medronic acid (Cat# 5191-3940, Agilent Technologies)
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bioRxiv - Plant Biology 2021Quote: ... Two µl of samples were introduced in the splitless injection mode in DB-5 (60 m × 0.25 mm, 1 µm film thickness, Agilent, CA) using helium as a carrier gas ...
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bioRxiv - Genetics 2021Quote: ... Analytes were separated on a HP-5 capillary column (50 m by 320 μm by 1.05 μm column; Agilent, USA). The carrier gas was helium (constant flow of 1.4 mL · min-1) ...
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bioRxiv - Biophysics 2020Quote: ... and the MFS0 peptide biotin-N-GGGGS (GPGGA)5 GGKYG GS-K(ε-N3) were purified via preparative C18 reverse phase HPLC (5 µm particle size, 250×21.2 mm, Agilent) applying a linear gradient of 0.1% triflouracetic acid (TFA ...
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bioRxiv - Evolutionary Biology 2020Quote: ... RNA probes were synthetized by cloning the DNA amplified region in the Strataclone PCR Cloning Kit (240205-5, Agilent Technologies) (hoxd12a ...
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bioRxiv - Immunology 2020Quote: 100μL of PRAK-03202 sample was injected onto Agilent Bio SEC-5 (5μ, 2000Å, 7.8 mm ID × 300mm) (Agilent Technologies) column for analytical SEC analysis and analysed with Agilent Technologies HPLC instrument (1260 Infinity II) ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were washed for 5 minutes using 1X Wash Buffer and then blocked by adding 8 drops of Protein Block Serum-Free (Agilent). Primary antibodies were diluted in diluent (Dako ...
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bioRxiv - Physiology 2020Quote: ... followed by a final elongation step at 72 °C for 5 min (SureCycler 8000, Agilent technologies, Santa Clara, CA, USA). In order to amplify different transcripts via nested PCR from the renin gene ...
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bioRxiv - Neuroscience 2020Quote: ... Supernatants were collected and analyzed using an Agilent HPLC system equipped with a Pursuit C18 column (150 ×4.6 mm, 5 µm; Agilent Technologies) operating at a flow rate of 1 ml/min or ...
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bioRxiv - Cancer Biology 2021Quote: ... - Kinetex EVO (5 μm, 100 Å)) using an HPLC system (Agilent, LC 1260 Infinity II, Agilent, Santa Clara, CA, USA). A two-step gradient was applied ...
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bioRxiv - Biophysics 2020Quote: ... coupled with a ZORBAX Eclipse AAA Analytical Guard Column (4.6 mm x 12.5 mm x 5 μm; Agilent #820950-931) heated at 40°C ...
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bioRxiv - Microbiology 2020Quote: ... equipped with a 30 x 0.25 mm (0.25 µm thickness) DB-5 MS capillary column (Agilent J & W capillary GC) and coupled to a Polaris Q benchtop Ion Trap Mass Spectrometer ...
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bioRxiv - Microbiology 2020Quote: Proteins were detected using the following primary antibodies: anti-enterovirus VP1 clone 5-D8/1 antibody purchased from Dako (Denmark); Mouse anti-Ub ...
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bioRxiv - Plant Biology 2021Quote: ... together with mass and retention time alignment (0.1 min and 5 ppm respectively) were done in Profinder B.07 software (from Agilent Technologies). Annotation was done based on the ‘find-by-formula’ algorithm ...