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Citations for Agilent :
6901 - 6950 of 9205 citations for Mouse Autophagy related protein 16 1 ATG16L1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Secretin receptor as a target in gastrointestinal cancer: expression analysis and ligand developmentbioRxiv - Cancer Biology 2022Quote: ... The brown staining was then generated by incubation of the sections with Liquid DAB+ Substrate Chromogene Kit (Dako, Glostrup, Denmark) for 2 min ...
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bioRxiv - Cancer Biology 2022Quote: ... The pCS2HA-HA-LIX1 plasmid was used as template to generate the LIX1 variants in which cysteine 83 and 84 were substituted by serine residues with the QuikChange Site-Directed Mutagenesis Kit (Stratagene) according to the manufacturer’s protocol and the primers listed in supplemental Table S1.
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bioRxiv - Microbiology 2022Quote: All of the indicated mutations were introduced into a plasmid backbone expressing His6 tagged pNL4-3-derived IN by QuikChange site directed mutagenesis kit (Agilent) (66) ...
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bioRxiv - Biophysics 2022Quote: ... The insertion of an amber codon (TAG) between E715 and V716 in mGluR2 (azi-ECL2) was performed using the QuikChange site-directed mutagenesis kit (Agilent). SNAP-mGluR2 constructs used for calcium imaging had C-terminal FLAG-tag removed by PCR-based deletion using phosphorylated primers ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Gα subunit plasmids were purchased from cdna.org (Bloomsburg University, Bloomsburg, PA. 4A insertion mutations were introduced to Gα subunits using the QuikChange Mutagenesis Kit (Agilent Technologies) and oligonucleotides (Integrated DNA Technologies ...
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bioRxiv - Microbiology 2022Quote: ... The quality and size distribution of the DNA was assessed on a Fragment Analyzer using the HS NGS Fragment Kit (Agilent). The pooled samples were then sequenced on a NovaSeq6000 (Illumina ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and Pyrrhocoris apterus samples with the Agilent RNA 6000 Pico Kit on a BioAnalyzer 2100 (Agilent, Santa Clara, United States). T ...
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bioRxiv - Neuroscience 2021Quote: ... GlyT2 was subcloned into pcDNA3 and the GlyT2 mutants were constructed by site-directed mutagenesis using the QuikChange kit (Stratagene). Plasmids from two independent Escherichia coli colonies were transfected into eukaryotic cells and [3H]glycine transport was measured in the cells for verification ...
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bioRxiv - Neuroscience 2021Quote: The fragment size of ChIP-seq and RNA-seq libraries was measured using high sensitivity DNA analysis kit (5067-4626, Agilent) on a TapeStation system (2200 ...
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bioRxiv - Neuroscience 2021Quote: ... Libraries were prepared using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina and verified using the Bioanalyzer RNA Nano Assay (Agilent) before sequencing on the NextSeq 500 (Illumina) ...
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bioRxiv - Plant Biology 2021Quote: ... Both RNA quality and concentration were checked using Agilent RNA 6000 Nano Kit Bioanalyzer chip (Agilent Technologies, St. Clara, USA). 5 µg of the purified RNA was used in the reverse transcription reactions using the SuperScript III First-Strand Synthesis System for RT-PCR (Life Technologies Cuisinart) ...
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bioRxiv - Microbiology 2020Quote: ... Libraries were quality checked for their size and concentration with electrophoresis using a High Sensitivity D1000 kit on a 2200 TapeStation (Agilent). Prepared samples were sequenced by the Center for Advanced Technology at the University of California ...
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bioRxiv - Plant Biology 2021Quote: ... BsaI restriction sites were removed by site-directed mutagenesis using the “QuickChange II Kit” following the manufacturer’s instructions (Agilent Technologies). Plasmid DNA amplification was performed by heat-shock transformation into Escherichia coli DH5α cells (10min on ice ...
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bioRxiv - Plant Biology 2021Quote: ... Final libraries were amplified with 17 cycles of PCR and assessed on the Bioanalyzer with the High Sensitivity DNA kit (Agilent). All root tissue libraries that were sequenced comprised two biological replicates.
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bioRxiv - Immunology 2021Quote: ... we changed three amino acids within the Fc region by using the QuikChange II site directed mutagenesis kits (Agilent, #200523), introducing M257Y ...
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bioRxiv - Microbiology 2020Quote: ... RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA).
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bioRxiv - Microbiology 2020Quote: ... DNA removal was confirmed by PCR using primers OL398 and OL399 (Table 2) and RNA quality was assessed using an Agilent 2100 Bioanalyzer system with corresponding RNA 6000 Nano kit (Agilent) to confirm RNA integrity (RIN) ...
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bioRxiv - Microbiology 2020Quote: ... and average fragment size of each sample was assessed using an Agilent 2100 Bioanalyzer system and RNA 6000 Nano kit (Agilent) prior to sequencing ...
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bioRxiv - Microbiology 2021Quote: ... and the pooled library was then quality checked and quantified with a Agilent Bioanalyzer 2100 using the DNA HS kit (Agilent). Sequencing of was performed using an Illumina MiSeq with a 300-cycle micro flow cell with V2 chemistry ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The quality was checked with an RNA 6000 Nano Chip on an Agilent Bioanalyzer 2100 (Kit #5067-1511, Agilent Technologies). All RIN-scores were higher than 5.4.
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bioRxiv - Neuroscience 2021Quote: RNA-seq libraries were prepared for each time-points and seady-state sample using the QuantSeq 3’ mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen) automated on the NGS WorkStation (Agilent) at The Centre for Applied Genomics (TCAG ...
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bioRxiv - Microbiology 2019Quote: All of the mutations were introduced into a plasmid backbone expressing His6 tagged pNL4-3-derived IN by QuikChange site directed mutagenesis kit (Agilent) [60] ...
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bioRxiv - Microbiology 2020Quote: ... Mutations to the NF-κB and IRF binding sites in pLTR(Sp1)-luciferase were generated using the QuickChange IIXL site-directed mutagenesis kit (Stratagene). Primers used for site-directed mutagenesis are listed in Table 1 ...
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bioRxiv - Molecular Biology 2020Quote: ... The quality of final single cell 5’ GEX libraries was assessed by Qubit quantification and fragment analysis (DNF-474 High Sensitivity NGS Fragment Analyzer Kit, Agilent). The sequencing was performed on a NextSeq500 device (Illumina ...
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bioRxiv - Genetics 2021Quote: ... RNA quality control (QC) was performed with the RNA 6000 Nano kit analysed using the 2100 Bioanalyzer Eukaryote Total RNA Nano assay (Agilent). cDNA libraries for sequencing were produced using the KAPA mRNA HyperPrep Kit for Illumina Platforms (Kapa Biosystems ...
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CRISPR-Cas9-mediated knockout of CYP79D1 and CYP79D2 in cassava attenuates toxic cyanogen productionbioRxiv - Plant Biology 2021Quote: ... The BsaI site in the backbone of the binary destination vector pCAMBIA2300 was removed via the QuikChange Site-Directed Mutagenesis Kit (Agilent) (Hajdukiewicz et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... the LdNT3-targeting construct shown in Figure S2D was modified with the QuikChange Site-directed Mutagenesis Kit (Stratagene, Lajolla, CA) using the primers listed in Table S2 ...
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bioRxiv - Plant Biology 2021Quote: ... RNA-seq libraries were constructed using a TruSeq Stranded mRNA Library Prep Kit and quantified on an Agilent bioanalyzer (Agilent) and single-end 125bp sequences generated on an Illumina Hi-seq 2500.
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bioRxiv - Neuroscience 2021Quote: Exonic sequences were enriched with the SureSelect Human All Exon 50□Mb V5 Kit (Agilent Technologies, Santa Clara, CA, USA). Sequences were generated on a HiSeq2500 (Illumina ...
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bioRxiv - Molecular Biology 2020Quote: The quality of 500 ng bisulfite-treated mRNA (see above) was assessed using Agilent RNA 6000 Pico Kit (Agilent, #NC1711873), and then subjected to NGS libraries preparation using VAHTS Stranded mRNA-seq Library Prep Kit (Vazyme) ...
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bioRxiv - Molecular Biology 2020Quote: ... F561A and K568A) were generated by site directed mutagenesis using the Quick-Change Lightning Site-Directed Mutagenesis Kit (Agilent Technologies) according to the manufacturer’s instructions.
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bioRxiv - Bioengineering 2020Quote: ... We measured the size distribution and concentration of each well in 96-well plate using Fragment Analyzer High-sensitivity NGS kit (Agilent). We normalized the concentration of cDNA of each well to a concentration of 0.4 ng/µL ...
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bioRxiv - Neuroscience 2021Quote: ... RNA from cultured and transfected DRG cells was purified using the Absolutely RNA Nanoprep Kit (Agilent Tech., Santa Clara, CA). RNA quantity was measured using a NanoDrop 1000 (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... and RNA quality was assayed on an Agilent 2100 Bioanalyzer using the Agilent RNA 6000 Pico Kit (Agilent Technologies, USA). Libraries were built using TruSeq Stranded Total RNA Library Prep Kits (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... Substitution mutations in the IDR were constructed by site-directed mutagenesis using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent). The pBad-Gfp-IDRFtsZ was constructed by cloning the coding sequence of Gfp-IDRFtsZinto the NheI and HindIII sites on the arabinose inducible vector ...
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bioRxiv - Cell Biology 2021Quote: ... and were mutated by deleting 6-7 base pairs using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies). The primers used for mutagenesis are listed in Supplementary Table S6 ...
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bioRxiv - Cell Biology 2021Quote: ... The quantity and quality of RNA samples were analyzed using Agilent RNA 6000 Nano kit with the bioanalyzer from Agilent. RNA samples with RNA Integrity Number (RIN ...
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bioRxiv - Cell Biology 2020Quote: ... All mutant constructs used in this study were generated from this construct either by a QuickChange II XL kit (Stratagene) or a Q5® Site-directed mutagenesis kit (New England Biolabs ...
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bioRxiv - Neuroscience 2021Quote: Total RNA was isolated and purified using the Absolutely RNA Miniprep Kit (Cat# 400800, Agilent Technologies, Santa Clara, CA, USA) according to the manufacturer’s protocol for extremely small samples ...
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bioRxiv - Neuroscience 2021Quote: ... KCNQ2 mutants were generated by site-directed mutagenesis using a QuikChange kit according to manufacturer’s protocol (Stratagene, San Diego, CA). cRNA transcripts encoding human KCNQ1-5 were generated by in vitro transcription using the T7 polymerase mMessage mMachine kit (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... after quality control using the Agilent 2100 Bio analyzer (Agilent RNA 6000 Nano Kit, Agilent Technologies, Santa Clara, CA, USA), poly-T oligo-attached magnetic beads were used to purify poly-A containing mRNA ...
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bioRxiv - Neuroscience 2021Quote: ... TDP-43N-Del was generated by deletion of the first 81 amino acids from the N-terminus of TDP-43WT using the QuickChange Site-Directed Mutagenesis Kit (Agilent).
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bioRxiv - Neuroscience 2021Quote: ... The 481 bp ywhaz amplicon was generated by PCR reaction and cloned into a plasmid using the StrataClone PCR cloning Kit (Agilent). The plasmids were collected and purified using GeneJET Plasmid Maxiprep Kit (Thermo Scientific ...
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bioRxiv - Genomics 2020Quote: ... quality of libraries was assessed using a high sensitivity Small DNA Fragment Analysis Kit (Agilent Technologies, Santa Clara, CA, USA) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Kinase dead catalytically inactive versions of Drosophila and human RIOK2 (dRIOK2123A,246A, RIOK2123A,246A) were created using site directed mutagenesis (Lightning Quick Change Kit, Agilent) to make alanine substitutions at catalytic residues Lys-123 and Asp-246 in both human RIOK2 and dRIOK2 as described by other groups (22 ...
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bioRxiv - Genomics 2019Quote: ... with an average fragment length of 625 bp (ranging from 300 to 2000 bp, measured on Bio Analyzer High Sensitivity DNA Kit, Agilent). The prepared library was sequenced on Illumina NovaSeq 6000 Sequencing system with S4 flow cells ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA library quality was quantified by 2100 Bioanalyzer using Agilent High Sensitivity DNA Kit (#5067-4626, Agilent, Santa Clara, CA). Library was sequenced as 101 bp paired-end on Illumina NovaSeq6000.
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bioRxiv - Biochemistry 2021Quote: ... Mutations of the two Walker-A motifs in human ABCF1 (K324M, K664M) were created by using Quikchange II Site Directed Mutagenesis Kit (Agilent). Human ABCF1 and yeast GCN20 domain fusion cDNAs were created by PCR-mediated ligation and cloned into modified pMtac-His6 vector ...
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bioRxiv - Immunology 2020Quote: ... The libraries were analyzed for size and quantity of DNAs on a 2100 Bioanalyzer using a High Sensitivity DNA kit (Agilent), diluted and pooled in multiplex sequencing pools ...
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bioRxiv - Molecular Biology 2022Quote: ... Final size selected libraries were checked for quality on the Agilent BioAnalyzer with High Sensitivity DNA analysis kit (5067-4626, Agilent) to confirm proper size selection ...