Labshake search
Citations for Agilent :
6801 - 6850 of 6919 citations for Nitrite Microplate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: The pEGFP-N1-CELF1 putative eIF4E binding mutants were generated via site-directed mutagenesis using the QuickChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies, La Jolla, CA) and primers listed in Supplementary Table 2 ...
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bioRxiv - Developmental Biology 2019Quote: We used the manufacturer protocol for the Agilent SureSelect Capture kit for non-human exomes with 200 ng gDNA per individual (75 Mb capture designed on the zv9 version of the zebrafish genome; Agilent SSXT Zebrafish All Exon kit; Agilent Technologies, Santa Clara, CA). Samples were multiplexed and run across two lanes of the Illumina HiSeq 4000 as paired-end 150 bp reads ...
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bioRxiv - Genomics 2021Quote: ... A mouse monoclonal anti-p63 antibody (1:500, MS-1081-P, Neomarkers, Fremont, CA, USA) and followed by a secondary kit (Mouse Envision, DAKO-Agilent, Santa Clara CA, USA), stained with chromogen (3,3′-Diaminobenzidine [DAB] ...
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bioRxiv - Neuroscience 2019Quote: ... and SNAREQ (SNAP25 K40A/D51A/E52A/E55A/D166A) were generated in the above described Syt1 and t-SNARE background respectively using the QuickChange mutagenesis kit (Agilent Technologies, Santa Clara, CA). All proteins were expressed and purified as described elsewhere (Mahal et al ...
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bioRxiv - Evolutionary Biology 2021Quote: Mutants in the VPg cistron were synthesized by long inverse site-directed PCR mutagenesis using the QuickChange II XL Kit (Stratagene, San Diego CA, USA) following the manufacturer’s instructions and using p35STunos as template ...
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bioRxiv - Biochemistry 2021Quote: ... plasmid phapXVENUS-hph (Gsaller et al., 2014) was used as template and modified using QuikChange II site-directed mutagenesis kit (Agilent for K38E and R50A) or Q5 site-directed mutagenesis kit (New England Biolabs ...
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bioRxiv - Neuroscience 2021Quote: ... The fragment distribution of each library was assessed the using the High Sensitivity DNA Kit on an Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA). Libraries were quantified prior to sequencing using the Qubit DNA High Sensitivity kit (ThermoScientific ...
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bioRxiv - Genomics 2021Quote: ... exome enrichment was applied to the cDNA purified from the previous step to enrich spliced cDNA by using probe kit SSELXT Human All Exon V8 (Agilent, catalog no. 5191-6879) and the compatible reagent kit SureSelectXT HSQ (Agilent ...
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bioRxiv - Immunology 2021Quote: cDNA was synthesized from the previously extracted RNA of mice colon tissue by using Affinity Script One-Step RT-PCR Kit (Cat# 600559, Agilent, Santa Clara, CA, USA). RNA was mixed with random 9mer primers ...
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bioRxiv - Molecular Biology 2021Quote: ... after the molecular cloning the wt sequence was restored by mutagenesis using the QuikChange Site-Directed Mutagenesis Kit (Agilent Technologies, Inc., Santa Clara, CA), the pETM11-CspA plasmid as DNA template and the mutagenic primers G670 5′-TTTCAGGGCGCCATGTCCGGTAAAATGACTG-3′ and G671 5′-CAGTCATTTTACCGGACATGGCGCCCTGAAA-3′.
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bioRxiv - Cell Biology 2021Quote: ... pCMV6-XL5-WT-SorLA759 or pCMV6-XL5-WT-SorLA2131 plasmids using the QuikChange II XL Site-Directed Mutagenesis Kit from Agilent (Santa Clara, CA, USA) according to manufactory’s instructions ...
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bioRxiv - Genomics 2021Quote: ... HMW DNA was also imaged with a gDNA 165 kb kit (FP-1002-0275) on the Femto Pulse system (Agilent, Santa Clara, CA, USA). A detailed procedure is deposited in protocols.io (dx.doi.org/10.17504/protocols.io.bafmibk6).
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bioRxiv - Neuroscience 2020Quote: The α10W55T mutant subunit was produced as previously described (Boffi et al., 2017) using Quick change XL II kit (Stratagene, La Jolla, CA, USA). The chimeric α10x construct was generated in two steps ...
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bioRxiv - Genomics 2022Quote: ... Quantity were assessed on a Qubit 2.0 Fluorometer using a Qubit RNA HS Assay kit and the quality were checked by capillary electrophoresis on an Agilent Bioanalyzer using the RNA 6,000 Pico LabChip kit (Agilent Technologies, Santa Clara, CA, USA). Purified RNA was stored at −80 °C until further use.
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bioRxiv - Neuroscience 2022Quote: ... These constructs were made by performing site-directed mutagenesis on the Syt9 cDNA with the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent, Santa Clara, CA, USA). Full-length CLM constructs were used for the rescue experiments in Fig ...
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bioRxiv - Cancer Biology 2022Quote: ... Genome-wide expression profiling was performed using the low-input QuickAmp labeling kit and human SurePrint G3 8×60K pangenomic microarrays (Agilent Technologies, Santa Clara, USA) as previously described (Merdrignac et al ...
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bioRxiv - Cancer Biology 2022Quote: We performed in-solution targeted enrichment of exonic sequences from spleens of two heterozygous MVD013 mice on C3H background together with six mutants and two wild-type controls from three other lines on the same genetic background using the SureSelectXT Mouse All Exon 50 Mb kit (Agilent Technologies, Santa Clara, CA). The generated libraries were indexed ...
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bioRxiv - Immunology 2022Quote: ... and Extracellular Acidification Rate (ECAR) were measured using Seahorse XF Cell Energy Phenotype kit on Seahorse XFp Flux Analyzer (Agilent Technologies, Santa Clara, CA) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... The quality of extracted RNA was verified using the Agilent 2100 Expert Bioanalyzer using the RNA 1000 Nano kit (Agilent Technologies, Santa Clara, CA). Ribosomal RNA depletion and library construction were performed using the RiboZero Plus kit and TruSeq stranded mRNA library preparation kit (Illumina ...
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bioRxiv - Physiology 2022Quote: ... Basal oxygen consumption rate and mitochondrial Oxidative Phosphorylation System (OXPHOS) parameters were measured according to manufacturer’s instructions of the Mito Stress test Kit (Seahorse Bioscience, Agilent Technologies, CA, USA). Results were normalized to protein quantified using a bicinchoninic acid-based protein assay (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2022Quote: ... and concentration prior to library generation with the Agilent RNA Pico 6000 kit on the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). rRNA depleted RNA passing quality control was used in the SMARTer Stranded RNA-Seq Kit (Takara Bio USA) ...
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bioRxiv - Neuroscience 2022Quote: ... The fragment distribution of each library was assessed using the High Sensitivity DNA Kit on an Agilent 2100 Bioanalyzer (Agilent Technologies; Palo Alto, CA). Libraries were quantified prior to sequencing using the Qubit DNA HS kit (ThermoScientific ...
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bioRxiv - Molecular Biology 2022Quote: ... amplified and labeled with Cyanine 3 (Cy3) as instructed by the manufacturer of the One-Color Agilent Low Input Quick Amp Labeling Kit (Agilent Technologies, Les Ulis, France). Cy3-labeled cRNA was hybridized onto Agilent Whole Human Genome Oligo 8×60K V2 Arrays (SurePrint G3 Human Gene Expression 8×60K v2 Microarray Kit ...
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bioRxiv - Molecular Biology 2022Quote: The reverse transcription reaction was performed on 0.4 µg of RNA template and cDNA was synthesized using an AffinityScript QPCR cDNA Synthesis Kit as instructed by the manufacturer (Agilent Technologies, Les Ulis, France). Quantitative PCR was performed using optimized TaqMan® gene expression assays (Applied Biosystems ...
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bioRxiv - Molecular Biology 2022Quote: ... of the cDNA was assessed on an Agilent Technologies Bioanalyzer 2100 using an Agilent DNA 1000 kit according to the manufacturer’s instructions (Agilent Technologies, Palo Alto, CA, USA).
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bioRxiv - Neuroscience 2022Quote: ... Quantity and quality of all RNA samples were determined using the Agilent RNA 6000 Nano Kit on the Agilent 2100 Bioanalyzer system (Agilent Technologies, Santa Clara, CA). Only samples with an RNA Integrity Number (RIN ...
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bioRxiv - Microbiology 2024Quote: ... Plasmids harboring mutated sRNA alleles under the control of the PLlac promoter were constructed using the QuikChange II XL site-directed mutagenesis kit (Agilent Technologies, Santa Clara, CA) with oligonucleotides that contain mismatched bases at the desired locations (Table S2 ...
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bioRxiv - Cancer Biology 2024Quote: ... Extracts from tumors and media were analyzed using the Absolute IDQ p180 kit (Biocrates Life Sciences AG, Innsbruck, Austria) with an Agilent 1290 Infinity UHPLC system (Agilent, Santa Clara, CA, USA) coupled with an MS/MS Sciex Triple Quad 6500 (AB Sciex LLC ...
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bioRxiv - Physiology 2024Quote: ... Etomoxir (an inhibitor of long chain fatty acid oxidation) provided in the Seahorse XF Mito fuel flex test kit (Agilent Technologies, Cat#103260-100). Fuel dependency for a specific pathway was measured after inhibiting this pathway ...
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bioRxiv - Neuroscience 2024Quote: ... was performed on blood and hiPSC genomic DNA using the SurePrint G3 Human CGH Microarray Kit 4 x 180K in accordance with the manufacturer’s instructions (Agilent Technologies, Palo Alto, CA, USA). Probe positions are referred to human genome assembly GRCh37/hg19.
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bioRxiv - Microbiology 2024Quote: ... The purity and quantity of the extracted RNA were analyzed using RNA 6000 Nano LabChip Kit and Bioanalyzer 2100 (Agilent CA, USA, 5067-1511). High-Quality RNA samples with RIN number > 7.0 were used to construct the sequencing library ...
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bioRxiv - Biochemistry 2024Quote: A genetic library was constructed based on the metX and metY genes with the GeneMorph II Random Mutagenesis Kit (Agilent, Santa Clara, CA, USA), adjusted to produce an average of 4 nonsynonymous mutations per gene ...
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bioRxiv - Cell Biology 2022Quote: ... The quality of the cDNA product was checked using an Agilent High Sensitivity DNA Kit and Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA) to ensure the complete removal of contaminants ...
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bioRxiv - Genetics 2023Quote: ... The purity and quantity of the extracted RNA were analyzed using RNA 6000 Nano LabChip Kit and Bioanalyzer 2100 (Agilent CA, USA, 5067-1511). High-Quality RNA samples with RIN number > 7.0 were used to construct the sequencing library ...
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bioRxiv - Neuroscience 2023Quote: ... labelled cRNA was prepared from 200 ng (mPFC/HT) or 40 ng (NAc) of total RNA using the One-Color Quick Amp Labelling kit (Agilent Technologies, Santa Clara, CA) according to the manufacturer’s instructions ...
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CXCL17 binds efficaciously to glycosaminoglycans with the potential to modulate chemokine signallingbioRxiv - Immunology 2023Quote: ... A panel of SUMO3-CXCL17 truncation mutants was generated by SDM by introducing stop codons at the required positions using the QuikChange II Site-Directed Mutagenesis Kit (Agilent Technologies; Santa Clara, USA).
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bioRxiv - Neuroscience 2023Quote: ... Libraries concentrations and size distributions were assessed by running samples on Agilent 2100 Bioanalyzer using the Agilent High Sensitivity DNA Kit (Agilent, Part Number 5067-4626). cDNA sample size modes were about 300 nucleotides (N.B. ...
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bioRxiv - Cancer Biology 2023Quote: ... samples were incubated with FSCN1 primary antibodies at 4°C overnight and the color of the sample was developed using the DAKO ChemMate Envision Kit HRP (Dako-Cytomation, Carpinteria, CA, USA) followed by counterstaining with hematoxylin ...
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bioRxiv - Cell Biology 2023Quote: ... S781A or S781D) were created by standard mutagenesis using QuickChange II XL Site-Directed Mutagenesis Kit (cat#200522, Agilent Technologies, Santa Clara, CA, USA). CDS of full-length Ctb ...
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bioRxiv - Cancer Biology 2023Quote: ... PD-L1 expression was assessed using the US Food and Drug Administration-approved Dako PD-L1 IHC 22C3 pharmDx kit (Agilent Technologies, Santa Clara, CA) in the samples ...
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bioRxiv - Neuroscience 2023Quote: ... and the average size of the libraries was determined using the High Sensitivity NGS fragment analysis kit on the Fragment Analyzer (Agilent Technologies; Santa Clara, CA). The normalized libraries were then sequenced on an Illumina NextSeq500 sequencing platform with 75-bp single-end reads for at least 20 million reads per sample in multiplexed sequencing experiments.
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bioRxiv - Microbiology 2023Quote: ... tissues were incubated with proteinase K diluted 1:10 in 1X TBS (Fisher Scientific, Cat. #BP24711; and PNA ISH kit, Agilent Dako, Cat. #K5201) for 10 min at RT in a humidity chamber ...
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bioRxiv - Cancer Biology 2023Quote: ... SCR_019417) was used for library size distribution analysis with a high-sensitivity NGS Fragment Kit (Cat# DNF-474-0500, Agilent, Santa Clara, CA, USA). qPCR for quantification was run using an Illumina-compatible KAPA Library Quantification Kits (ROX Low ...
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bioRxiv - Evolutionary Biology 2023Quote: ... A subset of the samples from each developmental stage was quality-checked by Bioanalyzer using the RNA 6000 Pico Kit (Agilent Technologies Catalog # 5067-1513). The resulting RNA integrity numbers were between 8.4 and 10 ...
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bioRxiv - Genomics 2023Quote: ... The quality of the cDNA libraries and final DNA libraries were analyzed using the 2100 Bioanalyzer Instrument with the High Sensitivity DNA Kit (Agilent, Santa Clara, CA, USA).
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bioRxiv - Pathology 2023Quote: ... RNA concentration was determined using an RNA 6000 Pico Kit run on a Bioanalyzer 2100 running Agilent 2100 Expert Software (B.02.11; Agilent Technologies, Santa Clara, CA, USA). RNA was converted to cDNA using iScript cDNA Supermix (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: ... The hybridization was performed according to the manufacturer instructions on 4×180K mouse microarrays (SurePrint G3 Mouse CGH Microarray Kit, 4x180K, AGILENT Technologies, reference genome: mm9). Microarrays were scanned with an Agilent High-Resolution C Scanner using a resolution of 3 µm and the autofocus option ...
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bioRxiv - Physiology 2023Quote: ... Standard curves for each gene were generated by cloning qPCR products into the pSCA vector with the Strataclone PCR cloning kit (Agilent, Santa Clara, CA, USA), isolating plasmid DNA using the GeneJET Plasmid Miniprep Kit (Thermo Scientific ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Aliquots of ∼0.1 g were used for total RNA (RNAt) extractions with the Agilent Plant RNA isolation Mini kit (Agilent Technologies, Santa Clara CA, USA). Three aliquots of RNAt per sample were separated and their concentrations adjusted to 50 ng μL−1.
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bioRxiv - Biochemistry 2023Quote: ... A mutant Acr library was made using ACX175 as a backbone and error-prone PCR with low mutation rate using GeneMorph II random mutagenesis kit (Agilent, Santa Clara, CA, USA). The resulting library was subcloned into a second plasmid containing AiEvo2 ...