Labshake search
Citations for Agilent :
6801 - 6850 of 8383 citations for Human Adenosylhomocysteinase Like 1 AHCYL1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... RNA quantity and quality were assessed using NanoDrop ND-1000 Spectrophotometer (Nanodrop Technologies) and Agilent 2100 Bioanalyser (RNA 6000 Nano LabChip kit, Agilent Technologies).
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LSD1 inhibition improves efficacy of adoptive T cell therapy by enhancing CD8+ T cell responsivenessbioRxiv - Immunology 2023Quote: Seahorse experiments were performed on sorted CD8+ cells activated in presence or absence of LSDi using XF Cell Mito Stress kit (Seahorse Bioscience). OCR and ECAR were measured with XF96 Extracellular Flux Analyzers (Seahorse Bioscience) ...
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bioRxiv - Immunology 2023Quote: ... Endogenous peroxidase was blocked using Peroxidase block (Refine Kit) followed by Protein block (X090930-2, Agilent Technologies Inc., Santa Clara, CA). Primary antibodies were applied at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... Two μl of RNA samples was used for reverse transcription with the AccuScript High-Fidelity 1st Strand cDNA Synthesis Kit (Agilent; 200820) using random hexamer primers ...
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bioRxiv - Immunology 2023Quote: ... The size of the resulting libraries was determined with the Agilent D1000 reagent kit and Agilent 4200 TapeStation (Agilent Technologies, USA). Pooling was performed automatically using a Tecan Freedom EVO robotic station (Tecan ...
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bioRxiv - Microbiology 2023Quote: ... The purified libraries were then assessed for their fragment sizes using the High Sensitivity DNA kit on the Bioanalyzer 2100 (Agilent Technologies). After applying the NetoVIR protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The quality of each library was assessed using a Bioanalyzer high sensitivity kit on an Agilent 2100 Bioanalyzer (Agilent 5067-4626). The libraries were submitted for sequencing at Cancer Research UK Cambridge Institute Genomic Core Facility using Illumina NovaSeq with 100bp single-end reads.
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bioRxiv - Cancer Biology 2023Quote: ... Single-cell RNA-seq and CMO libraries were prepared according to the manufacturer’s protocol and the library quality was confirmed with a Bioanalyzer High-Sensitivity DNA Kit (Agilent, 5067-4627). Cellecta barcode libraries were prepared using 25% of the adaptor-ligated cDNA to amplify only the barcoded cDNA product ...
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bioRxiv - Cell Biology 2023Quote: ... Quantification and quality checked of libraries was done using an Agilent 2100 Bioanalyzer instrument and a DNA 7500 kit (Agilent Technologies). Libraries were pooled and sequenced on a NovaSeq6000 S1 100 cycle run (v1.5 SBS reagents) ...
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bioRxiv - Cell Biology 2023Quote: The NGS sample was diluted with TE buffer and assayed using a High Sensitivity DNA Kit (cat. #5067-4626, Agilent Technologies) on a Bioanalyzer 2100 ...
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bioRxiv - Biochemistry 2023Quote: Mutations were generated by site-directed mutagenesis (table 2) on this plasmid using QuikChange II Site-Directed Mutagenesis Kit (Agilent Technologies) and plasmids were verified through sequencing through the Molecular Informatics Core at the University of Rhode Island.
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bioRxiv - Biochemistry 2023Quote: ... The desired PP1 or PNUTS mutants were generated by oligonucleotide- mediated site-directed mutagenesis (QuikChange II XL Site-Directed Mutagenesis Kit, Agilent Technologies) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Site-specific mutagenesis was performed according to standard procedures of the QuickChange Site-Directed Mutagenesis Kit purchased from Stratagene (GE Healthcare) or Mut Express II (Vazyme Biotech) ...
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bioRxiv - Biophysics 2023Quote: ... H80S/D83S and G93A mutants were generated by the use of QuikChange Site-Directed Mutagenesis Kit (Stratagene, La Jolla, CA, USA) (60) ...
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bioRxiv - Plant Biology 2023Quote: ... Protease activity of NIa-Pro was abolished by mutating cysteine 151 to alanine (C151A) using the same construct and the QuikChange Lightning Site-Directed Mutagenesis kit (Agilent, USA) in both pMDC32 and pSITE (Huogen et al. ...
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bioRxiv - Genomics 2023Quote: ... and RNA integrity was checked with an RNA Kit on an Agilent 5300 Fragment Analyzer (Agilent Technologies, Palo Alto, CA, USA). RNA sequencing libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina following the manufacturer’s instructions (NEB ...
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bioRxiv - Pathology 2024Quote: ... and the integrity of the RNAs was verified using the Agilent 2100 bioanalyzer with the RNA 6000 Nano kit (Agilent Technologies). 1 µg of total RNA was reverse transcribed in a 20 µL μnal reaction volume using the High-Capacity Reverse Transcription kit (Applied Biosystems ...
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bioRxiv - Cell Biology 2024Quote: ... The INF2-ER[K792A] GFP construct was generated via site-directed mutagenesis using the QuikChange II Site-Directed Mutagenesis Kit (Agilent Technologies). All constructs were sequenced completely across their coding region.
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bioRxiv - Genetics 2024Quote: ... was used for all experiments with integrated Cas9 (Supplementary Table 3).25 Cas9D10A was generated by site-directed mutagenesis of pLS504 using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent #200521) using primers MK11 and MK12 ...
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bioRxiv - Cell Biology 2024Quote: ... The S71A and S71E mutations were introduced into the pDONR221-gCDC42attb plasmid with the QuickChange II XL Site Directed Mutagenesis Kit (#200521, Agilent Technologies) with the following primers ...
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bioRxiv - Cell Biology 2024Quote: ... The integrity and quantity of the total RNA was measured using an Agilent 2100 Bioanalyzer RNA 6000 Nano Kit (Agilent Technologies). Total RNA obtained from each sample was subjected to sequencing library construction using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs ...
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bioRxiv - Cell Biology 2024Quote: ... The S71A mutation was introduced into the pMALc-MBP::CDC-42 plasmid with the QuickChange II XL Site directed mutagenesis kit (#200521, Agilent technologies) with the following primers ...
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bioRxiv - Cell Biology 2024Quote: The aforementioned mutations in the hypervariable regions of RhoA-GFP and RhoB-GFP were generated according to the manufacturing instructions of the QuickChange Lightning Site-Directed Mutagenesis kit (Agilent Technologies) using the mutagenic primers listed in the Key Resources Table ...
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bioRxiv - Developmental Biology 2024Quote: ... The quality and concentration of the libraries were assessed using the Agilent High Sensitivity DNA Kit and Bioanalyzer 2100 (Agilent Technologies) and submitted to 150 bp paired-end high throughput sequencing using Hiseq4000 (Illumina).
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bioRxiv - Biophysics 2024Quote: OCR measurements were performed with the Agilent Seahorse XF Cell Mito Stress Test kit with an XF24 extracellular flux analyser (Seahorse Bioscience). XF24 plates were coated with either 23 µl of 100% Matrigel (soft ...
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bioRxiv - Cell Biology 2024Quote: ... Mutations were constructed by site-directed mutagenesis according to the instructions accompanying the Quik Change ® Site-Directed Mutagenesis Kit (Agilent Technologies, Stratagene). Conventional PCRs reactions were performed with KAPA Taq DNA polymerase (Kapa Biosystems ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Purified RNA samples were analyzed for quality control using an Agilent 2100 Bioanalyzer and an RNA 6000 Nano kit (Agilent Technologies). Microarray experiments were performed using a custom-designed Agilent 8 × 60 K array for the E ...
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bioRxiv - Microbiology 2024Quote: ... in which the glutamic acid in the active site was substituted by alanine employing QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent Technologies) and appropriate primers listed in SI appendix ...
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bioRxiv - Immunology 2024Quote: ... Detection was achieved using Leica DAB Detection Kit (Bond Polymer Refine Detection) for CD4 and Envision FLEX DAB+ (Diaminobenzidine) (Dako Omnis) for BCL6 ...
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bioRxiv - Microbiology 2024Quote: ... To assess quality and size of the libraries samples were run on an Agilent Bioanalyzer 2100 using an Agilent High Sensitivity DNA Kit as recommended by the manufacturer (Agilent Technologies). Concentration of the libraries was determined using the Qubit® dsDNA HS Assay Kit as recommended by the manufacturer (Life Technologies GmbH ...
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bioRxiv - Immunology 2024Quote: The integrity of the isolated RNA (according to the aforementioned protocol) was measured with a Fragment Analyzer using RNA Kit 15 nt (Agilent Technologies). Samples with an RNA integrity number ≥ 8 were used for sequencing ...
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bioRxiv - Microbiology 2024Quote: ... Site-directed mutagenesis of the Trp252 residue in BoGH5A was performed using the QuikChange II Site Directed Mutagenesis (SDM) Kit (Agilent Technologies) according to the manufacturer’s protocol.
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bioRxiv - Zoology 2024Quote: ... RNA integrity was verified using an Agilent 2100 Bioanalyzer and an RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA). RIN values ≥ 7.0 were considered appropriate for mRNA library preparation ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Purified total RNA samples (100 ng) were labeled with Cyanine3 (Cy3) using a Low Input Quick Amp WT labeling kit (One-color; Agilent Technologies). The Cy3-labeled cRNA samples were checked for amount (>825 ng ...
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bioRxiv - Developmental Biology 2024Quote: ... HySp5-2992-mBS4:Luc and HySp5-2992-mBS5:Luc constructs were generated using the QuikChange Lightning Multi Site-Directed Mutagenesis Kit (Agilent Technologies).
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bioRxiv - Plant Biology 2024Quote: ... thaliana cpSRP54 residues 1-564 including a point mutation at position 185 (At-cpSRP54(Q185R)) was generated using the QuikChange Lightning site-directed mutagenesis kit (Agilent Technologies) using pCBi18-At-cpSRP54 as template ...
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bioRxiv - Microbiology 2024Quote: ... The size distribution of the libraries was checked by high-resolution gel electrophoresis with a Bioanalyzer instrument using the DNA 7500 Pico kit (Agilent Technologies). Libraries were quantified through fluorometry with a QubitTM fluorometer and dsDNA HS reagents (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2024Quote: ... Genomic DNA libraries were constructed using the TruSeq Nano DNA kit and quality-checked in the TapeStation D1000 ScreenTape System (Agilent Technologies). Genomic libraries were sequenced on an Illumina NovaSeq with PE150 (paired-end ...
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bioRxiv - Genomics 2024Quote: ... RNA samples were quantified and RNA integrity numbers (RIN) derived using the Bioanalyzer High Sensitivity RNA 6000 Pico kit (Agilent, UK).
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bioRxiv - Genomics 2024Quote: ... and the RNA integrity number (RIN) was measured using a tape-station system with an RNA screen-tape analysis kit (Agilent Technologies).
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bioRxiv - Cell Biology 2024Quote: ... The ATP production from mitochondrial respiration and glycolytic respiration in response to Oligomycin and Rotenone/Antimycin A was measured using the ATP production assay kit (Agilent, USA). The oxygen consumption rate (OCR ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR products were purified with Agencourt AMPure XP beads and sample profile was analysed with the High Sensitivity D5000 kit (Agilent Technologies) on a TapeStation (Agilent Technologies) ...
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bioRxiv - Developmental Biology 2024Quote: ... GHTF assessed the quality and quantity of the amplified cDNA by electrophoresis using Agilent’s Agilent High Sensitivity DNA Kit (Agilent: 5067-4626) and Bioanalyzer before library construction ...
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bioRxiv - Cancer Biology 2021Quote: ... Libraries were prepared following the manufacturer’s instructions (Agilent HaloplexHS for 1-500kb target region). Briefly ...
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bioRxiv - Molecular Biology 2021Quote: ... template WT plasmid was digested by the addition of 1 μL DpnI (Agilent Technologies) for 5 min at 37 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... the sections were incubated with the biotinylated secondary antibody (1:300, DAKO, Glostrup, Denmark) for 1 h at room temperature ...
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bioRxiv - Bioengineering 2022Quote: ... and primary antibody against GFAP (rabbit anti-GFAP, 1:100, Dako, Z0334, Carpinteria, CA) or CX43 (rabbit anti-CX43 ...
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bioRxiv - Biochemistry 2019Quote: XL-1 and BL21(DE3)pLysS strains of E.coli were purchased from Stratagene (USA) and a previously described vector pGEMEX-1(his6 ...
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bioRxiv - Neuroscience 2019Quote: ... for the glial cell marker S100β with rb anti-S100 (Agilent Dako; 1:1000) and for nuclei with DAPI (4’,6-Diamidino-2-Phenylindole Dihydrochloride ...
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bioRxiv - Neuroscience 2019Quote: ... for the glial cell marker S100β with rb anti-S100 (Agilent Dako; 1:1000) and for nuclei with DAPI (4’,6-Diamidino-2-Phenylindole Dihydrochloride ...