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Citations for Agilent :
6601 - 6650 of 9205 citations for Mouse Autophagy related protein 16 1 ATG16L1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... Site-directed mutagenesis of WT SIRT5 was performed using the QuickChange Lightning site-directed mutagenesis kit (#210518, Agilent Technologies), according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2023Quote: Introduction of specific mutations in the SOSIP Envs was performed using QuikChange site-directed mutagenesis kit (Agilent Technologies, USA) according to manufacturer’s instructions and verified by sequencing analysis (Eton Bioscience ...
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bioRxiv - Genomics 2023Quote: ... and its quality was spot-checked for random samples using the Bioanalyzer High Sensitivity RNA Kit (Agilent 5067-1513). 100 ng of total RNA was quantified using the Qubit RNA High Sensitivity Assay (ThermoFisher Q32852 ...
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bioRxiv - Microbiology 2023Quote: ... RNA integrity was assessed on an Agilent 2100 Bioanalyzer using the RNA 6000 nano kit (Agilent, Santa Clara, CA) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The average library size for the pool was determined using an Agilent DNA D1000 Tape Station kit (Agilent #5067).
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bioRxiv - Cancer Biology 2023Quote: ... their fragment size was analyzed by High Sensitivity NGS Fragment Analysis Kit (DNF-474) on a Fragment AnalyzerTM (Agilent). Libraries were pooled and sequenced with Illumina technology with paired-end reads of 50bp length.
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bioRxiv - Biochemistry 2023Quote: ... Gly241Glu and Asp235Ala:Gly241Glu were introduced into the previously published vectors (5) using the QuickChange II Site-directed mutagenesis kit (Agilent) using primers listed in Table S2.
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bioRxiv - Biophysics 2023Quote: ... The Pro283Ser amino substitution was introduced to this vector via the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent) as previously described (13) ...
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bioRxiv - Neuroscience 2023Quote: ... The fragment size of the final libraries was determined using the Bioanalyzer High-Sensitivity DNA Kit (Agilent, CA, USA). Library concentration was determined using the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... and RNA integrity was checked with RNA Kit on Agilent 5300 Fragment Analyzer (Agilent Technologies, Palo Alto, CA, USA).
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bioRxiv - Neuroscience 2023Quote: ... The fragment size of the final libraries was determined using the Bioanalyzer High-Sensitivity DNA Kit (Agilent, CA, USA). Library concentration was determined using the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2024Quote: ... These mutations were introduced into pJW1 by site-directed mutagenesis using the QuikChange II kit (Agilent Technologies, Cat #200523) to make pJW1-KR>DD and pJW1-KR>AA ...
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bioRxiv - Cell Biology 2024Quote: ... Carbonyl cyanide-p-trifluoromethoxyphenylhydrazone (FCCP) and Rotenone/Antimycin A was measured using the mitochondrial stress test kit (Agilent, USA). The glycolytic activity or glycoPER in response to Rotenone/Antimycin A and 2-deoxy-D-glucose was measured using glycolytic rate assay (Agilent ...
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bioRxiv - Developmental Biology 2024Quote: ... Point mutations were introduced into sel-5 and dpy-23 sequences using the QuikChange Site-directed Mutagenesis kit (Agilent). Transcriptional reporter Ppgp-12::gfp was created by inserting 3410 bp of pgp-12 promoter upstream of GFP in pPD95.81 ...
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bioRxiv - Cancer Biology 2024Quote: ... Blood T cell fractions were estimated using TcellExTRECT using exons from the corresponding capture kit (Agilent custom V2 Exome) with a median coverage threshold set to five.
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bioRxiv - Physiology 2024Quote: ... for 24h then incubated in the Seahorse XF media (+/- 0.25mM succinate) and processed using the Cell Mito Stress Test Kit (#103015 Agilent).
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bioRxiv - Biochemistry 2024Quote: ... MicroRNAs (miRs) were polyadenylated and then re-verse-transcribed using the miR 1st-Strand cDNA Synthesis Kit (Agilent Technologies) according to manufacturer’s protocol.
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bioRxiv - Physiology 2024Quote: ... and the quality of the libraries was checked with a 2100 Bioanalyzer with a high-sensitivity DNA kit (Agilent). Libraries were sequenced using a P3 flow cell on a NextSeq2000 in 100 bp paired-end mode (Illumina ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA samples were run on an Agilent 2100 bioanalyzer using the RNA 6000 Pico LabChip kit (Agilent, Diegem, Belgium). The bioanalyzer electropherograms were analyzed by Agilent 2100 Expert Software to determine the RNA quantity and quality ...
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bioRxiv - Neuroscience 2024Quote: ... the library preparations were sequenced on an Illumina Hiseq Ten platform with 150 bp paired end module.The panel kit was customized from Agilent Technologies ...
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bioRxiv - Neuroscience 2024Quote: ... The quality and concentration of the RNA was assessed with Agilent RNA 6000 Pico Kit (Agilent, Cat#5067-1513) on Bioanalyser 2100 (Agilent) ...
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bioRxiv - Neuroscience 2024Quote: ... The fragment size of the final libraries was determined using the Bioanalyzer High-Sensitivity DNA Kit (Agilent, CA, USA). Library concentration of the final libraries was determined using the Tape Station High Sensitivity D1000 ScreenTape (Agilent ...
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bioRxiv - Cell Biology 2024Quote: ... The Agilent SureSelect Strand Specific mRNA library kit was used as per the manufacturer’s instructions (Agilent, Santa Clara, CA). Library construction began with two rounds of polyA selection using oligo dT containing magnetic beads ...
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bioRxiv - Cell Biology 2024Quote: ... Target enrichment was performed to construct the exome library using the Agilent SureSelect Human All Exon V6 kit (Agilent Technologies ...
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bioRxiv - Cell Biology 2024Quote: ... Sequencing libraries were validated using NGS Kit on the Agilent 5300 Fragment Analyzer (Agilent Technologies, Palo Alto, CA, USA), and quantified by using Qubit 4.0 Fluorometer (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... of SspH1 was generated in the pcDNA3::2xHA-SspH1 backbone using the Quikchange II site-directed mutagenesis kit according to the manufacturer’s protocol (Agilent). Briefly ...
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Braf-mutant Schwann cells divert to a repair phenotype to induce congenital demyelinating neuropathybioRxiv - Developmental Biology 2024Quote: ... The individual libraries were assessed using a Qubit™ and the Agilent Bioanalyzer dsDNA High Sensitivity Kit (Agilent Technologies). We sequenced at 2*76 base-pairs on a NovaSeq 6000 (Illumina ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The final libraries were then quality-checked and quantified for multiplexing using the Bioanalyzer High-Sensitivity DNA kit (Agilent) and Qubit dsDNA HS Assay kit (Thermo Scientific) ...
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bioRxiv - Immunology 2024Quote: ... PCR fragments were purified with Qiagen MinElute PCR Purification Kit and library concentration and quality were determined by Agilent High Sensitive DNA Kit and TapeStation ...
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Genetic gradual reduction of OGT activity unveils the essential role of O-GlcNAc in the mouse embryobioRxiv - Developmental Biology 2024Quote: ... The integrity of the RNA was determined using the TapeStation 4150 (High Sensitivity RNA kit, Agilent Technologies #5067-5579). Library preparation was performed by EMBL Genomics Core Facility using the TruSeq Stranded mRNA Library Preparation kit according to the manufacturer’s instructions (Illumina #20020594) ...
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Genetic gradual reduction of OGT activity unveils the essential role of O-GlcNAc in the mouse embryobioRxiv - Developmental Biology 2024Quote: ... The integrity of the RNA was determined using the TapeStation 4150 (High Sensitivity RNA kit, Agilent Technologies #5067-5579). Library preparation was performed by EMBL Genomics Core Facility using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760L) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Amino acid deletions for ΔKPQ were generated by use of the QuikChange Lightning Site-Directed Mutagenesis kit (Agilent, USA). PCR products were cloned into XL-10 Gold Ultracompetent cells ...
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A humoral immune response to parasitoid wasps in Drosophila is regulated by JAK/STAT, NF-κB and GATAbioRxiv - Immunology 2024Quote: ... The library quality was assessed using a Bioanalyzer high sensitivity kit on an Agilent 2100 Bioanalyzer (Agilent #5067-4626). The libraries were sequenced at Source BioScience using Illumina NovaSeq 150bp paired-end reads.
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bioRxiv - Neuroscience 2024Quote: ... RNA integrity was assessed with 1µl of each mRNA-purified sample using RNA 6000 Pico kit (Agilent, 5067-1513) on an Agilent Bionalyzer with RNA Integrity Number (RIN ...
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bioRxiv - Molecular Biology 2024Quote: ... Two µL of each diluted sample were run using the HS Large Fragment 50kb Kit #DNF-464-0500 (Agilent) according to the manufacturer’s instructions on the 5200 Fragment Analyzer System # M5310AA ...
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bioRxiv - Biophysics 2024Quote: ... The resulting WGBS libraries were evaluated on a 2100 Bioanalyzer using Agilent High-Sensitivity DNA Kit (Agilent #5067-4626) and quantified via qPCR using the KAPA Library Quantification Kit (KAPA #KK4824) ...
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bioRxiv - Neuroscience 2024Quote: ... Quality and concentration of extracted RNA was assessed using a Tapestation 2100 with the High Sensitivity RNA kit (Agilent) for samples extracted form sorted somata and using a 2100 Bioanalyzer with the RNA 6000 Pico kit (Agilent ...
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bioRxiv - Molecular Biology 2024Quote: ... The Agilent QuikChange Primer Design Program and the QuikChange II Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, USA) were utilized to incorporate point mutations into the coding sequence of human GAT1 ...
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bioRxiv - Cancer Biology 2021Quote: ... according to the manual with 1 ng/ml Ki-67 antibody (M728; Dako). For color reaction ...
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bioRxiv - Evolutionary Biology 2019Quote: ... with 1 µl PfuTurbo Cx hotstart polymerase (2.5 U/µl, Agilent Technologies, USA), 5 µl 10X PfuTurbo Cx reaction buffer ...
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Weak Membrane Interactions Allow Rheb to Activate mTORC1 Signaling Without Major Lysosome EnrichmentbioRxiv - Cell Biology 2019Quote: ... and transformed into XL-1 Blue Supercompetent Cells (Agilent Technologies, Santa Clara, CA). Plasmid DNA was isolated from multiple colonies and sequenced.
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bioRxiv - Cancer Biology 2019Quote: ... the slides were incubated with biotin coupled goat anti-rabbit (1:500, Dako). Subsequently ...
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bioRxiv - Neuroscience 2020Quote: ... We used the following primary antibodies: rabbit anti-GFAP at 1:3,000 (Dako), rabbit anti-S100B at 1:500 (Dako) ...
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bioRxiv - Neuroscience 2020Quote: ... GFAP was detected with rabbit anti-GFAP (1:1000, Z0334, Agilent, CA, USA), HA-tagged DREADD receptors were detected with goat anti-HA (1:1000 ...
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bioRxiv - Plant Biology 2021Quote: ... Each sample (1 μl) was separated on a gas chromatograph (7820A; Agilent Technologies) combined with a mass spectrometric detector (5977B ...
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bioRxiv - Cancer Biology 2021Quote: ... was diluted 1:25 in Antibody Diluent with Background Reducing Components (Dako, S3022). 200 µL of antibody solution was put on each slide to cover all TMA specimens and incubated overnight at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: 3μm thick FFPE sections were stained for CD8 (1:20, C8/144B, Dako) on a Roche Ventana autostainer ...
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bioRxiv - Cancer Biology 2022Quote: ... membranes were probed with either horseradish peroxidase-conjugated secondary antibodies (DAKO; 1:10,000) or IRDye 680- and 800-conjugated secondary antibodies (LI-COR ...
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bioRxiv - Cancer Biology 2022Quote: ... these slides were incubated with rabbit anti-human PGP 9.5 (DAKO 1:100) overnight at 4°C to mark the nerve fibers ...
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bioRxiv - Bioengineering 2022Quote: ... Additional heart sections were stained with anti-α-SMA (1:75 dilution, Dako) and Alexa Fluor-647 (1:500 dilution ...