Labshake search
Citations for Agilent :
6551 - 6584 of 6584 citations for Human Short Coiled Coil Protein SCOC ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... according to the manufacturer protocol and complementary DNAs were generated using an AccuScript High Fidelity 1st Strand cDNA Synthesis kit (Agilent Technologies, Santa Clara, VA, USA). A quantitative PCR was performed using a QuantStudio™ 3 Real-Time PCR system (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2020Quote: ... RNA integrity was defined by capillary electrophoresis with an RNA 6000 Nano Lab-on-a-Chip kit and Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA). Only if the integrity number values of RNA were more than 6 ...
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bioRxiv - Microbiology 2019Quote: ... the material was loaded onto denaturing polyacrylamide gel electrophoresis (dPAGE) at 12% or analysed using the Agilent RNA 6000 Pico LabChip kit on an Agilent 2100 Bioanalyzer (Agilent Technology, Palo Alto, CA, USA). Controls were carried out under the same conditions ...
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bioRxiv - Microbiology 2020Quote: Single point SCoV2-NTD-Fc N-linked glycosylation site mutants (N61, 74, 122, 149, 165, 234, 282Q) were generated using a QuikChange site-directed mutagenesis kit (Agilent Technologies, Santa Clara, CA, USA) to change the respective asparagine (N ...
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bioRxiv - Microbiology 2020Quote: ... The synthesis and fluorescence labeling of cDNA followed a strand-specific method using the FairPlay III Microarray Labeling Kit (Agilent Technologies, Santa Clara, CA, USA) and actinomycin D (Calbiochem ...
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bioRxiv - Microbiology 2020Quote: ... and D67N/K70R/T215F/K219Q viruses were generated by site-directed mutagenesis on an xxLAI HIV-1 backbone using the QuikChange XL Site-Directed Mutagenesis kit (Agilent Technologies Inc, Santa Clara, CA), according to the manufacturer’s protocols.
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bioRxiv - Immunology 2020Quote: ... and Ii L17A was subsequently used as template for PCR quick change mutagenesis (all reagents used were included in the kit; QuickChange® Site-Directed Mutagenesis (Stratagene, La Jolla, CA, USA)) in order to generate the AP-3 binding motif RRP 21 ...
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bioRxiv - Immunology 2021Quote: ... was performed on an Illumina HiSeq 4000 machine using libraries prepared with a SureSelectXT Mouse All Exon capture kit (Agilent Technologies, Santa Clara, CA, USA), to produce 150 paired end reads sufficient for a mean bait coverage of at least 150x ...
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bioRxiv - Immunology 2020Quote: ... The RNA Integrity Number (RIN) of all RNA samples were determined using an Agilent 2100 Bioanalyzer and Agilent RNA 6000 nano kit (Agilent Technologies, Santa Clara, CA, USA). A minimum RIN of 7.5 was used as the standard for inclusion in the gene expression study ...
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bioRxiv - Cancer Biology 2022Quote: ... of matched-PBMC DNA was fragmented using the SureSelect XT HS and XT Low Input Enzymatic Fragmentation Kit following the manufacturer’s instructions (Agilent, Santa Clara, USA, cat # 5191-4080). Molecular-barcoded libraries were constructed following the SureSelect XT HT targeted enrichment protocol for Illumina paired-end multiplexed sequencing libraries (Version A1 ...
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bioRxiv - Cancer Biology 2022Quote: Deletion of TBE consensus sites in the FOXM1 promoter was accomplished using the QuickChange XL Site-Directed Mutagenesis44 kit (Agilent Technologies, Santa Clara, CA, USA) by following manufacturer’s instructions and was then cloned into the pGL3 basic vector ...
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bioRxiv - Pathology 2022Quote: ... The RNA Integrity Number (RIN) of all samples was quantified using an Agilent 2100 Bioanalyser and Agilent RNA 6000 nano kit (Agilent Technologies, Santa Clara, CA, USA). RIN values above 7.5 were used as the inclusion criterion for subsequent gene expression analysis ...
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bioRxiv - Neuroscience 2022Quote: ... RNA quality was evaluated by measuring the RNA integrity number (RIN) using an Agilent RNA 6000 Nano Kit with an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... We constructed the HPRT1-I42T cDNA from this clone by site-directed mutagenesis using the Quick Change II Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA, USA). Primers sequences used to create the mutation are shown in Table S3 ...
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bioRxiv - Neuroscience 2024Quote: ... The EGFP and mCherry dynamin-2 mutations A618T and S619L were constructed by site-directed mutagenesis using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA, USA). Neuropeptide Y fused to mCherry (NPY-mCherry ...
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bioRxiv - Evolutionary Biology 2024Quote: VPg mutations D113G and R118H were introduced by site-directed mutagenesis in the p35STunos plasmid using the Quikchange II XL kit (Agilent Technologies, Santa Clara CA, USA) and following the indications of the manufacturer ...
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bioRxiv - Cancer Biology 2024Quote: ... The quality and integrity of the total RNA were determined using the Agilent 2100 Bioanalyzer and the RNA 6000 Nano LabChip Kit (Agilent, Santa Clara, CA, United States). A sequencing RNA library was constructed by performing a 3′-end linker ...
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bioRxiv - Microbiology 2023Quote: K65R and M184V mutations in RT were generated by site-directed mutagenesis on an HXB2 or LAI HIV-1 backbone using the QuikChange XL site-directed mutagenesis kit (Agilent Technologies, Inc., Santa Clara, CA) according to the manufacturer’s protocols ...
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bioRxiv - Plant Biology 2023Quote: ... The quality of the total RNA extracted from target cells was evaluated using the RNA 6000 Pico kit on an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). RNA integrity was determined based on the RNA Integrity Number (RIN ...
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bioRxiv - Genomics 2023Quote: ... we performed 6 cycles of PCR amplification on the remaining indexed DNA.The size distribution of the resulting library was monitored using a Fragment Analyzer with the High Sensitivity NGS kit (Agilent Technologies, Santa Clara, CA, USA) and the library was quantified using microfluorimetry (Qubit dsDNA HS kit ...
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bioRxiv - Genomics 2023Quote: ... The size distribution of the resulting library was monitored using a Fragment Analyzer with the High Sensitivity NGS kit (Agilent Technologies, Santa Clara, CA, USA) and the library was quantified using the KAPA Library quantification kit (Roche ...
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VapC12 ribonuclease toxin modulates host immune response during Mycobacterium tuberculosis infectionbioRxiv - Microbiology 2023Quote: ... PCR products are then purified and checked for fragment size distribution on Fragment Analyzer using HS NGS Fragment Kit (1-6000bp) (Agilent, catalog no. DNF-474-1000). Genes with low expression value (mean FPKM value<0.5 ...
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bioRxiv - Cancer Biology 2023Quote: ... was used for inhibiting glycolysis (and associated ECAR) and was obtained from the Seahorse XF Glycolytic Rate Assay Kit (Agilent Technologies 103344-100. FCCP (5μM) was used to reveal maximal respiratory capacity (and associated OCR ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The library was quality controlled by analysis on an Agilent 2000 Bioanalyzer with the Agilent High Sensitivity DNA Kit (Agilent Technologies, Santa Clara, CA, USA) for fragment sizes of ca ...
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bioRxiv - Cancer Biology 2022Quote: A library of the entire genomic sequence of all 50 known genes (electronic supplementary Table S1) was prepared using HaloPlex Target Enrichment kits (Agilent Technologies, Santa Clara, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... RNA quantity and purity were determined using a NanoDrop™ spectrophotometer (ND-1000, ThermoScientific™, Delaware, USA) and an Agilent 2100 Bioanalyzer with the RNA 6000 Nano Kit (Agilent Technologies, CA, USA) using the 2100 Expert Software to calculate RNA Integrity Number (RIN ...
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bioRxiv - Pathology 2023Quote: ... A mutant minigene with the c.1066-11G>A variant was generated by site-directed mutagenesis with QuikChange Lightning Kit (Agilent Technologies, Santa Clara, CA, USA). Sanger sequencing confirmed the identity of the constructs ...
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bioRxiv - Microbiology 2024Quote: ... RNA quality and integrity were assessed via chip gel electrophoresis with a 2100 Bioanalyzer and an RNA Nano chip kit (Agilent Technologies, Santa Clara, CA, USA).
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bioRxiv - Cancer Biology 2024Quote: ... Between 3 to 6 sections of each tumor were also immunostained for Ki67 (mouse anti-ki67, BD, San Jose CA, USA; blocking reagent, M.O.M ImmPRESS kit, Vector Laboratories, Burlingame CA, USA; liquid DAB+, Dako North America Inc, Carpinteria CA, USA), digitized (Nanozoomer ...
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bioRxiv - Zoology 2024Quote: ... The quality and quantity of the libraries were checked on an Agilent 2100 Bioanalyzer using the Agilent DNF-935 Reagent Kit (Agilent Technologies, Santa Clara, CA, USA). Libraries were pooled to equimolar amounts ...
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bioRxiv - Plant Biology 2021Quote: ... Quality and integrity of RNA was assessed with the Fragment Analyzer from Advanced Analytical (Heidelberg, Germany) by using the standard sensitivity RNA Analysis Kit (Agilent, Santa Clara, CA, USA; DNF-471). All samples selected for sequencing exhibited an RNA integrity number over 8 ...
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bioRxiv - Cancer Biology 2023Quote: ... eluted in 20 µL EB buffer and traces were evaluated on the Fragment Analyzer using the HS Large Fragment kit (Agilent Technologies, cat. no. DNF-492-0500) at 1:5 dilutions ...
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bioRxiv - Microbiology 2021Quote: ... Concentration of RNA was checked on Thermo Scientific™ NanoDrop™ and the quality with Agilent 2100 BioAnalyzer™ System using the appropriate cassette (Agilent RNA 6000 Nano Kit). 10 µg of isolated RNA was treated with DNase for 30 min at 37°C and ethanol precipitated overnight at −20°C ...
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bioRxiv - Microbiology 2022Quote: ... It was quantified using Quant-iT™ RiboGreen® RNA Assay Kit (Thermo) and its integrity was evaluated in a 2100 Bioanalyzer pico chip series (Agilent Technologies, Santa Clara, CA, USA).