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Citations for Agilent :
6401 - 6450 of 6584 citations for Human Short Coiled Coil Protein SCOC ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... quantitation and sample purity were assessed using the RNA Nano 6000 Assay Kit of the Agilent Bioanalyzer 2100 system (Agilent Technologies, CA, USA) and agarose gel electrophoresis ...
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bioRxiv - Cancer Biology 2023Quote: ... The oxygen consumption rate (OCR) assay was performed using the XF long-chain fatty acid oxidation (LCFA) stress test kit (Agilent, cat# 103672-100) and Seahorse XFe24 Analyzer was used to investigate the long-chain fatty acid OCR according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2023Quote: ... and leucine (Leu41) within the conserved destruction box were mutagenized to alanine (RxxL-> AxxA) using the Quickchange mutagenesis kit (Stratagene, La Jolla, CA). Worms were transformed with the pJK3 plasmid carrying gfp::ify-1dm using microparticle bombardment as previously described (Praitis et al. ...
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bioRxiv - Cell Biology 2023Quote: ... Total amounts and integrity of RNA were assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). Total RNA was used as input material for RNA sample preparations ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA concentration and quality was measured using the RNA 6000 Pico kit on the Bioanalyzer 2100 capillary electrophoresis system (Agilent, Santa Clara, CA) and stored at −80°C ...
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bioRxiv - Genetics 2024Quote: ... poly(A)+ RNA was separated and purified from total RNA using an oligo(dT) cellulose column Poly(A) Quick mRNA Isolation Kit (Stratagene, La Jolla, CA). Single-stranded cDNAs were synthesized by reverse transcription reaction using 0.25 U AMV reverse transcriptase (Life Sciences Advanced Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... while the concentration and integrity of RNA samples were detected by using Agilent 2100 RNA nano 6000 assay kit (Agilent Technologies, CA, USA). Subsequently ...
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bioRxiv - Biophysics 2024Quote: Introduction of the 118C mutation into the T4L gene (pET11a vector) was accomplished using the QuikChange site-directed mutagenesis kit (Agilent; Santa Clara, CA) and confirmed by DNA sequencing ...
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bioRxiv - Cell Biology 2024Quote: ... Mutations were constructed by site-directed mutagenesis according to the instructions accompanying the Quik Change ® Site-Directed Mutagenesis Kit (Agilent Technologies, Stratagene). Conventional PCRs reactions were performed with KAPA Taq DNA polymerase (Kapa Biosystems ...
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bioRxiv - Cancer Biology 2024Quote: The oxygen consumption rate (OCR) of cells was measured using the Seahorse Cell Mito Stress Test kit following the user guide (Agilent, cat. 103010-100). Briefly ...
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bioRxiv - Biochemistry 2024Quote: ... Average fragment lengths of 40-50 kbp were confirmed using a Fragment Analyzer (Advanced Analytical) with an HS Large Fragment 50 Kb analysis kit (Agilent, DNF-464-33). Samples were prepared for Oxford Nanopore Technologies (ONT ...
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bioRxiv - Microbiology 2024Quote: ... To determine the RNA integrity number the isolated RNA was run on an Agilent Bioanalyzer 2100 using an Agilent RNA 6000 Nano Kit as recommended by the manufacturer (Agilent Technologies, Waldbronn, Germany). Remaining genomic DNA was removed by digesting with TURBO DNase (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... Library qualities were assessed using the Agilent High Sensitive DNA Kit (#5067-4626) on an Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, USA). Qualified libraries underwent paired-end sequencing on an Illumina NovaSeq System (Illumina ...
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bioRxiv - Cell Biology 2024Quote: ... a DeNovix DS-11 FX spectrophotometer and an Agilent FEMTO Pulse System with a Genomic DNA 165 kb Kit (Agilent, FP-1002-0275), respectively ...
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bioRxiv - Cell Biology 2024Quote: ... and RNA integrity was assessed using the Agilent TapeStation 4200 and the Agilent RNA ScreenTape Assay Kit (Agilent; 5067-5576 and 5067-5577).
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bioRxiv - Plant Biology 2024Quote: ... The quality of the barcoded cDNA libraries was assessed with the High Sensitivity DNA Analysis Kit in the Agilent 2100 Bioanalyzer (Agilent Technologies, United States) and then sequenced (150 bp paired-end reads ...
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bioRxiv - Genetics 2024Quote: ... RNA concentration and quality was assessed using an Agilent 2100 Bioanalyzer with the RNA Nano 6000 kit (Agilent Technologies, Santa Clara, CA, USA), and only RNA samples with RIN >7 were processed for subsequent cDNA library construction and sequencing ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA integrity (RIN) was determined using the RNA Nano 6000 Assay Kit with the Agilent Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA, USA). RIN values ranged from 8.2-10 for all samples ...
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bioRxiv - Genetics 2021Quote: ... RNA integrity (RIN) was determined using the RNA Nano 6000 Assay Kit with the Agilent Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA, USA). RIN values ranged from 7.3–10 for all samples ...
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bioRxiv - Developmental Biology 2020Quote: ... analysis was performed using TransStart Tip Green qPCR SuperMix kit (TransGen Co.) on a Stratagene Mx3000P real-time PCR system (Stratagene, La Jolla, CA, USA) under the conditions of 94 °C for 30 s ...
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bioRxiv - Genomics 2019Quote: ... and successful size-selection verified by electrophoresis on an Agilent TapeStation using the Genomic DNA High ScreenTape kit (Agilent Technologies, Santa Clara, CA, USA).
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Standard quality control steps were included to determine total RNA quantity and quality using Agilent 2100 Bioanalyzer with Eukaryote Total RNA Pico Kit and High Sensitivity DNA Assay (Agilent Technologies, Santa Clara, CA). Libraries were prepared using the TruSeq Stranded mRNA Library preparation Kit (Illumina Inc. ...
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bioRxiv - Cancer Biology 2020Quote: ... early and late nascent strands were labeled with Cy3 and Cy5 ULS molecules using the Genomic DNA labeling Kit (Agilent, Santa Clara, CA, USA) following manufacturer's instructions ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Plasmid DNA mutations in the predicted orthosteric binding site of PAR4 were generated using the QuikChange XL Multi Site-Directed Mutagenesis Kit (Agilent Technologies, Mississauga, ON, Canada) to generate all mutants described in this study ...
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bioRxiv - Physiology 2019Quote: ... RNA quality and integrity were validated using a NanoDrop ND-1000 UV–vis Spectrophotometer (NanoDrop Technologies, Wilmington, USA) and Agilent 2100 Bioanalyzer and RNA 6000 Nano LabChip kit (Agilent Technologies, Palo Alto, USA) respectively ...
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bioRxiv - Immunology 2019Quote: Various N-linked glycosylation site mutants of UL144 were generated by site-directed mutagenesis using Quick Change II site directed mutagenesis Kit (Stratagene, La Jolla, CA, USA). The Asn residues carrying the N-linked glycan were mutated individually or in combination at residues N61 ...
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bioRxiv - Neuroscience 2019Quote: ... The resulting supernatants were transferred to new tubes and 50 µl of each cleared lysate was mixed with 50 µl Lysis Buffer (0.7 µl β-mercaptoethanol/100 µl Lysis Buffer; Agilent Absolutely RNA Nanoprep Kit) and stored at –80° for later preparation as input RNA ...
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bioRxiv - Genomics 2021Quote: ... Libraries from all sample sets were quantified on the 2100 Bioanalyzer instrument with the Agilent DNA 7500 Kit (both Agilent Technologies, Santa Clara, CA).
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bioRxiv - Bioengineering 2022Quote: ... Size pattern was assessed with Fragment Analyzer-96 with the DNF-474-0500 High Sensitivity NGS Fragment Analysis Kit (Agilent Technologies, DNF-474-0500). Libraries (Average size ...
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bioRxiv - Neuroscience 2020Quote: ... the purity and concentration of RNA samples were determined by readings of OD260/280 using a dual beam UV spectrophotometer and RNA integrity was determined by capillary electrophoresis using the RNA 6000 Nano Lab-on-a-Chip kit and the Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA) following the manufacturers instructions ...
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bioRxiv - Biochemistry 2020Quote: ... and Ile-residues of the HXXXDX motif of ZmGL2 and ZmGL2-LIKE were generated using QuikChange XL Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA). The ZmGL2 protein was also expressed in E ...
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bioRxiv - Systems Biology 2020Quote: ... The sub-libraries were each resuspended to a final volume of 20 μL in nuclease-free water and fragment distribution was checked running a bioanalyzer (Agilent High Sensitivity DNA Kit) according to the manufacturer’s protocol.
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bioRxiv - Plant Biology 2021Quote: ... The fragment sizes and concentrations of the pooled samples were determined on a Fragment analyzer 5200 using the DNF-473-Standard Sensitivity NGS Fragment Analysis Kit (Agilent, Santa Clara, CA, USA). The indexing PCR was performed under the following conditions ...
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bioRxiv - Neuroscience 2020Quote: ... The resulting supernatants were transferred to new tubes and 50 μl of each cleared lysate was mixed with 50 μl Lysis Buffer (0.7 μl β-mercaptoethanol/100 μl Lysis Buffer; Agilent Absolutely RNA Nanoprep Kit) and stored at −80°C for later preparation as Input RNA ...
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bioRxiv - Microbiology 2020Quote: ... Libraries were pooled in equal quantity and combined to multiplex to make a final library and QC was done again using Qubit quantification kit and Agilent bioanalyzer using high sensitivity DNA chip (Agilent Biotechnology, catalog # 5067-4626). The final combined library was sequenced using Illumina technology on NextSeq 500 sequencer using High output 300-bp Single end Read sequencing kit.
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bioRxiv - Plant Biology 2021Quote: ... Other related alleles required for transient expression or infection analyses were either created using the QuickChange Site-directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA) or synthesised by Twist Bioscience (South San Francisco ...
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bioRxiv - Neuroscience 2022Quote: ... with the standard-sensitivity RNA chip or an Agilent 2100 Bioanalyzer system with the RNA 6000 Nano Labchip Kit (Agilent Biotechnologies, Palo Alto, USA) was used to analyze the extracted RNA samples ...
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bioRxiv - Genomics 2022Quote: ... The obtained library quality and concentration was assessed by monitoring the distribution of the fragment sizes with a Bioanalyzer 2100 instrument using RNAnano reagent kit (Agilent Technologies, Santa Clara, CA). This size and quantity information was used for pooling the libraries in equimolar concentrations to normalize each library ...
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bioRxiv - Neuroscience 2022Quote: ... and RNA quality was assessed by determining RNA integrity numbers (RINs) using the Agilent 2100 Bioanalyzer system with the Agilent RNA 6000 Nano Chip Kit (Agilent Technologies, Santa Clara, California).
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bioRxiv - Immunology 2022Quote: ... for fragment size using the High Sensitivity NGS Fragment Analysis kit on a Fragment Analyzer capillary electrophoresis system (catalog # DNF-474-0500; Agilent, Santa Clara, CA, USA), and functional validation using the Universal Library Quantification kit (catalog # 07960140001 ...
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bioRxiv - Immunology 2022Quote: ... and for integrity using the Standard Sensitivity RNA Analysis kit on a Fragment Analyzer capillary electrophoresis system (catalog # DNF-471-0500; Agilent, Santa Clara, CA, USA). A total of 250ng total RNA was used for each sample as input to the TruSeq Stranded Total RNA library prep kit with unique-dual indexing (catalog #s 20020598 & 2002371 ...
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bioRxiv - Plant Biology 2022Quote: ... First-strand cDNA was synthesized from 0.3 μg of total RNA using the Affinityscript qPCR cDNA Synthesis Kit (Agilent Technologies, Santa Clara, CA, US). qPCR was performed using the Brilliant III Ultra-Fast SYBR Green QPCR Master Mix (Agilent Technologies ...
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bioRxiv - Developmental Biology 2022Quote: ... Mutations were generated within the miR-1 seed sequences using the QuikChange Lightning or QuikChange Multi Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, California) to disrupt miR-1’s binding and regulatory function (Gregory et al. ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA extraction was performed using a FinePure Plant RNA Kit (Genfine Biotech Co. Ltd., Beijing, China) and the qualities were assessed using a Bioanalyzer 2100 (Agilent, Palo Alto, CA, USA). A cDNA library was constructed using a NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (New England Biolabs ...
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bioRxiv - Neuroscience 2022Quote: ... RNA integrity (RIN) was assessed using a Bioanalyzer 2100 instrument in conjunction with the RNA 6000 Nano LabChip kit (Agilent Technologies, Santa Clara, California).
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bioRxiv - Plant Biology 2022Quote: ... The RNA quality was assessed using both the NanoPhotometer ® spectrophotometer (IMPLEN, CA, USA) and the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). Sequencing libraries were constructed using NEB Next® UltraTM RNA Library Prep Kit for Illumina (NEB ...
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bioRxiv - Genomics 2022Quote: ... RNA samples were then quantified and checked for RNA integrity using an Agilent BioAnalyzer 2100 with RNA Nano 6000 kit (Agilent Technologies, Santa Clara, USA), and only samples with an RNA integrity number ≥8 were sent for RNA-Seq ...
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bioRxiv - Cell Biology 2022Quote: ... I15K/R54P) were generated by mutagenesis of pcDNA-3XFLAG-TRIM25 using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA), while the TRIM25-PTAA mutant was generated using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA integrity (RIN) was determined using the RNA Nano 6000 Assay Kit with the Agilent Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA, USA). RIN values were > 7 for all samples ...
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bioRxiv - Neuroscience 2021Quote: ... The quality and quantity of the extracted RNA samples were analyzed with a 2100 Bioanalyzer using an RNA 6000 Pico Kit (Agilent, Santa Clara, CA, USA). Paired-end cDNA libraries were prepared from 0,2 ng of extracted RNA ...