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Citations for Agilent :
601 - 650 of 2124 citations for Parvalbumin PVALB cDNA ORF Clone Mouse N His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... OPG2-E was generated by cloning the cDNA encoding the E-protein into the same pcDNA5-OPG2 vector using the KpnI and BamHI sites and deleting the stop codon after the OPG2 tag by site-directed mutagenesis (Stratagene QuikChange, Agilent Technologies). The N-terminal OPG2-tag of OPG2-ORF6-OPG2 was inserted by site-directed mutagenesis of ORF6-OPG2 using the relevant forward and reverse primers (Integrated DNA Technologies) ...
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bioRxiv - Cancer Biology 2020Quote: All DNA panel and antibody tag libraries were run on a Bioanalyzer High Sensitivity DNA electrophoresis chip (Agilent Technologies, cat. no. 5067-4626) to verify complete removal of primer-dimer products ...
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bioRxiv - Biophysics 2020Quote: ... A thrombin site was introduced between the 6xHis-tag and the coiled-coil using the QuikChange Lightning mutagenesis kit (Agilent, Santa Clara, USA). Sequences of the products were confirmed by DNA sequencing ...
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bioRxiv - Microbiology 2022Quote: ... and H249A were generated from the parent pLVX Kernow C1/p6 ORF1AAAPG-Ha tag-AAAPG IRES zsGreen construct via QuikChange XL site-directed mutagenesis kit (Stratagene, La Jolla, CA). using primers listed in (Supplementary Table 1).
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bioRxiv - Synthetic Biology 2022Quote: ... 5’- CTT TTT CAA ATT GGG GGT GTG ACC ATG CAG CGC TAG CCG CAG C −3’) using the GeneMorph II epPCR kit (Agilent, Singapore, Cat: STR_200550). The epPCR profile is as follows ...
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bioRxiv - Biochemistry 2020Quote: Skd3 variants were expressed as an N-terminally MBP-tagged protein in BL21 (DE3) RIL cells (Agilent). Cells were lysed via sonication in 40mM HEPES-KOH pH=7.4 ...
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bioRxiv - Microbiology 2020Quote: ... and n-caproate concentrations from the pH experiment were analyzed with an Agilent 7890B Gas Chromatograph (Agilent Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... Japan) 41 with 2x hemagglutinin (HA) epitopes on the N-terminus into the pAAV-MCS vector (Agilent) and AAV9 was produced by Vigene or the Howard Hughes Medical Institute Viral Vector Core ...
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bioRxiv - Cell Biology 2024Quote: ... 15000 cells were seeded in biological replicates (n=4) into 96 well seahorse cell culture microplates (Agilent) pre-treated with fibronectin (5 μg/mL) ...
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bioRxiv - Neuroscience 2024Quote: ... the filter contents were injected into a gas chromatography (GC, Agilent 6890 N, Santa Clara, CA, USA) capillary column (DB-MS1 ...
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bioRxiv - Genetics 2024Quote: ... 25 µL of each sample was then transferred to the polypropylene inserts (Agilent P/N 5182-0549) in amber vials immediately before the LC-MS/QTOF run ...
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bioRxiv - Cell Biology 2024Quote: Respirometry of intact cells (n = 5–8 per cell line) was measured with a Seahorse Analyzer (Agilent) following the manufacturer’s guidelines ...
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bioRxiv - Synthetic Biology 2020Quote: ... The hybridisation mix was prepared by mixing the specified amounts of RNA with 10× Gene Expression Blocking Agent (1× final conc.) and 2× Hi-RPM Hybridization Buffer (1× final conc.) (Agilent, Cat No. 5188-5242) in a final volume of 42 μl ...
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bioRxiv - Molecular Biology 2024Quote: ... equipped with a refraction index (Surveyor RI Plus and Surveyor) on a 300 × 7.7 mm PL Hi-Plex H+ (8 μm particle size) column (Agilent Technologies, Santa Clara, CA, USA) and 4 × 3 mm ID Carbo-H guard (Phenomenex ...
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bioRxiv - Cancer Biology 2021Quote: ... Amplified cDNA quality was monitored with a high sensitivity DNA chip (Agilent) using the Bioanalyzer (Agilent) ...
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bioRxiv - Cell Biology 2020Quote: ... The quality of the cDNA was confirmed using Agilent Bioanalyzer (Agilent, USA) and RNAseq libraries were prepared using in-house compatible Tn5 and Nextera index primers (Illumina ...
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bioRxiv - Immunology 2021Quote: ... The cDNA quality and concentration was determined with the Fragment Analyzer (Agilent). For library preparation ...
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bioRxiv - Neuroscience 2020Quote: ... and the amplified cDNA quantified using a BioAnalyzer High Sensitivity Chip (Agilent). The cDNA was then fragmented and amplified for 3’ prime end sequencing with the Nextera XT DNA sample prep kit (Illumina) ...
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bioRxiv - Neuroscience 2020Quote: ... Amplification of cDNA was performed using Herculase II Fusion DNA polymerase (Agilent). Identity of amplified PCR products was verified by direct sequencing.
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bioRxiv - Neuroscience 2020Quote: ... The cDNA product was validated using an Advanced Analytical fragment analyzer (Agilent) with the HS NGS 1-6000 fragment kit (Agilent DNF-474) ...
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bioRxiv - Neuroscience 2022Quote: ... the amplified cDNA products of each single nucleus were quantified by Agilent Bioanalyzer 2100 ...
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bioRxiv - Genetics 2022Quote: ... cDNA was amplified using RT-PCR with Brilliant III SYBR Green (Agilent) as per the manufacturer’s instructions using primers ...
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bioRxiv - Genomics 2020Quote: ... cDNA was amplified using RT-PCR with Brilliant III SYBR Green (Agilent) as per manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... and reverse transcribed using AffinityScript Multiple Temperature cDNA Synthesis Kit (Agilent Technologies). Real-time quantitative PCR was performed with GoTaq qPCR Master Mix (Promega ...
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bioRxiv - Genetics 2020Quote: ... The cDNA quality and concentration was determined with the Fragment Analyzer (Agilent). Samples were subjected to library preparation (TruePrep DNA library Prep Kit V2 for Illumina ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA quality was evaluated by fragment analysis (5200 Fragment Analyzer System, Agilent). RNA sequencing was carried out using the Illumina NovaSeq System.
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bioRxiv - Developmental Biology 2023Quote: ... The cDNA quality and concentration were determined using a Fragment Analyzer (Agilent).
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bioRxiv - Microbiology 2023Quote: ... cDNA library quality was assessed using a High Sensitivity DNA Kit (Agilent). Finally ...
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bioRxiv - Microbiology 2023Quote: ... cDNA library quality was assessed using a High Sensitivity DNA Kit (Agilent). cDNA libraries were purified using the QIAquick PCR Purification Kit (QIAGEN ...
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bioRxiv - Molecular Biology 2022Quote: ... The human ARRB2 cDNA was cloned into pCMV-3Tag-8 (Agilent Technologies). The plasmid contains three copies of a FLAG epitope tag fused in-frame to the 3’ end of the ARRB2 cDNA ...
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bioRxiv - Molecular Biology 2022Quote: ... First strand of cDNA is synthesized using AffinityScript RT Enzyme (Agilent, 600107) according to the manufacturer’s instructions at 54? for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: ... Quality control of cDNA and libraries was conducted using a Bioanalyzer (Agilent) at the Stanford Protein and Nucleic Acid Facility ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was synthesized with AffinityScript One-Step RT-PCR Kit (Agilent, 600188). Each cDNA sample was amplified in triplicate using the SYBR Green and Platinum Taq polymerase (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2024Quote: ... cDNA of JUv1580_vlc was obtained using AccuScript High-Fidelity Reverse Transcriptase (Agilent) and reverse primers at the 3’ end of the genome ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The quality of the cDNA was tested on a TapeStation 2200 (Agilent) with D1000 Screen Tapes from the Agilent RK6 ScreenTape System ...
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bioRxiv - Immunology 2023Quote: ... cDNA was quantified on an Agilent Bioanalyzer High Sensitivity chip (Agilent Technologies), and 100 ng was used for library preparation ...
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bioRxiv - Immunology 2024Quote: ... The cDNA quality was assessed by bioanalyzer (Agilent, High Sensitivity DNA chip). 2 nanograms of amplified cDNA were used for our custom tagmentation protocol and indexed with Nextera XT primers ...
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bioRxiv - Immunology 2022Quote: ... Isolated RNA was reverse-transcribed with the cDNA synthesis kit (Agilent Technologies), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Quality control of cDNA and libraries was conducted using a Bioanalyzer (Agilent) at the Stanford Protein and Nucleic Acid Facility ...
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bioRxiv - Cell Biology 2024Quote: ... The quality of the cDNA libraries were assessed on the TapeStation (Agilent) and the libraries were sequenced using Illumina Nextseq 2000 platform with a target of 25million reads per sample.
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bioRxiv - Developmental Biology 2024Quote: ... cDNA quality was examined at multiple points on a 4200 TapeStation (Agilent) for yield and quality assessment ...
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bioRxiv - Microbiology 2024Quote: ... Obtained cDNA libraries were purified by AMPure XP and quantified by Agilent 2100 Bioanalyzer system (Agilent) ...
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bioRxiv - Microbiology 2024Quote: ... cDNA of JUv1580_vlc was obtained using AccuScript High-Fidelity Reverse Transcriptase (Agilent) and reverse primers at the 3’ end of the genome ...
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bioRxiv - Immunology 2024Quote: ... Library and cDNA quality were evaluated using a Bioanalyzer 2100 Instrument (Agilent) and quantifed using a ViiA 7 Real-Time PCR system (TermoFisher) ...
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bioRxiv - Cancer Biology 2024Quote: ... Full length cDNAs were cleaned and assayed (Agilent TapeStation, Santa Clara, CA) to ensure library lengths between 200-5000bp ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA was assessed using a Bioanalyzer High Sensitivity DNA Analysis assay (Agilent) and the libraries were quantified using the Collibri™ Library Quantification Kit (Thermo Fischer Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA and libraries fragments were profiled by automated electrophoresis (Tapestation 4200, Agilent) and High Sensitivity D5000 reagents and fluorometry using High Sensitivity 1x dsDNA reagents (Qubit ...
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bioRxiv - Developmental Biology 2024Quote: ... The cDNA generated from cell lysates were quantified by Bioanalyzer (Agilent Technology) to check cDNA concentration and quality ...
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bioRxiv - Developmental Biology 2024Quote: ... The cDNA quality and concentration were determined using a Fragment Analyzer (Agilent).
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bioRxiv - Neuroscience 2020Quote: ... followed by a mouse secondary antibody (rabbit polyclonal anti-mouse biotin antibody, E0413, Dako, 1:200). Histopathological studies of GBM xenograft mice were conducted by Division of Neuropathology ...