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Citations for Agilent :
501 - 550 of 2124 citations for Parvalbumin PVALB cDNA ORF Clone Mouse N His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... exome enrichment was applied to the cDNA purified from the previous step to enrich spliced cDNA by using probe kit SSELXT Human All Exon V8 (Agilent, catalog no. 5191-6879) and the compatible reagent kit SureSelectXT HSQ (Agilent ...
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bioRxiv - Molecular Biology 2022Quote: The reverse transcription reaction was performed on 0.4 µg of RNA template and cDNA was synthesized using an AffinityScript QPCR cDNA Synthesis Kit as instructed by the manufacturer (Agilent Technologies, Les Ulis, France). Quantitative PCR was performed using optimized TaqMan® gene expression assays (Applied Biosystems ...
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bioRxiv - Cell Biology 2020Quote: ... and Pnut with a C-terminal Strep tag II (WSHPQFEK, 1058 Da) were purified from Escherichia coli BL21(DE3) cells (Agilent Technologies) using a two-tag affinity purification scheme to aid the isolation of full-length complexes as explained in ref ...
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bioRxiv - Biochemistry 2021Quote: ... OPG2-E was generated by cloning the cDNA encoding the E-protein into the same pcDNA5-OPG2 vector using the KpnI and BamHI sites and deleting the stop codon after the OPG2 tag by site-directed mutagenesis (Stratagene QuikChange, Agilent Technologies). The N-terminal OPG2-tag of OPG2-ORF6-OPG2 was inserted by site-directed mutagenesis of ORF6-OPG2 using the relevant forward and reverse primers (Integrated DNA Technologies) ...
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bioRxiv - Microbiology 2023Quote: ... AIP56D274S/D276-278S and AIP56D274N/D276-278N with or without V5-tag were generated with the QuickChange Site-Directed Mutagenesis Kit (Stratagene, 200518) following the manufacturer’s instructions ...
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Development of ketobenzothiazole-based peptidomimetic TMPRSS13 inhibitors with low nanomolar potencybioRxiv - Pharmacology and Toxicology 2024Quote: ... R560 and K561 and addition of a 6xHis tag at the c-terminal using the QuikChange Lightning site-directed mutagenesis kit (Agilent, 210518).
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bioRxiv - Cancer Biology 2020Quote: ... cDNA libraries were assayed for quality control by Bioanalyzer (Agilent Technologies ...
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bioRxiv - Microbiology 2022Quote: ... First-strand complementary DNA (cDNA) was synthesized with Accuscript (Agilent) primed with a gene-specific oligonucleotide ...
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bioRxiv - Biochemistry 2020Quote: ... and cDNA was reverse transcribed with high fidelity Accuscript (Agilent) primed with gene-specific oligonucleotides ...
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bioRxiv - Immunology 2021Quote: ... cDNA was assessed and quantified using the Bioanalyzer 2100 (Agilent) and per sample 5 ng was used as template to generate sequencing libraries ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA libraries were assessed using 2100-Bioanalyzer (Agilent Technologies, USA) before 100 bp single-end sequencing using NextSeq500 (Illumina ...
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bioRxiv - Immunology 2021Quote: ... and cDNA quality was assessed using a Fragment Analyzer (Agilent). cDNA was tagmented with in-house Tn5 63 ...
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bioRxiv - Neuroscience 2020Quote: ... from cDNA fragments cloned in pBluescript II SK (Stratagene, USA). For Nissl staining adult brains were cryo-sectioned at −20°C (20 µm ...
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bioRxiv - Cancer Biology 2020Quote: ... Both cDNA and libraries were quantified using the TapeStation (Agilent) and sequenced on an Hiseq4000 (Illumina) ...
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bioRxiv - Cell Biology 2022Quote: ... cDNA and library traces were evaluated on a TapeStation (Agilent) using High Sensitivity D5000 and D1000 Screen Tapes (Agilent) ...
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bioRxiv - Cancer Biology 2021Quote: ... and cDNA concentration was determined with a Bioanalyzer (Agilent Technologies). cDNA libraries were prepared as outlined by the Single Cell 3’ Reagent Kits v2 user guide with modifications to the PCR cycles based on the calculated cDNA concentration.
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bioRxiv - Genomics 2020Quote: Labelled cDNAs were hybridised to oligonucleotide microarrays manufactured by Agilent as described (Rallis et al ...
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bioRxiv - Cell Biology 2020Quote: ... Flag-ErbB3 cDNA was subjected to site-directed mutagenesis (Agilent) to generate LL866/7AA mutant Flag-ErbB3 ...
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bioRxiv - Cell Biology 2022Quote: ... cDNA quality and quantity were tested with a Bioanalyzer (Agilent) using DNA High Sensitivity chips ...
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bioRxiv - Immunology 2022Quote: ... Cleanup cDNA was checked via a tape station (Agilent 42000) HSD5000 (cat# part # 5067-5593 ...
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bioRxiv - Neuroscience 2022Quote: ... The quality of cDNA was assessed by Bioanalyzer chip (Agilent) and concentration quantified by Qubit assay (Invitrogen) ...
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bioRxiv - Neuroscience 2024Quote: ... Final cDNA libraries were QC’d on the Tapestation 4200 (Agilent). Samples that passed library preparation were normalized to 10 nM and pooled using Starlet liquid handler ...
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bioRxiv - Molecular Biology 2024Quote: ... The resulting cDNA was amplified in an AriaMX cycler (Agilent) with the ITaq Universal SYBR Green Supermix and ROX as reference dye (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... RNA and cDNA sizes were assessed using a Bioanalyzer (Agilent). After reverse transcription and template-switching as described in the ONT protocol ...
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bioRxiv - Neuroscience 2023Quote: ... The cDNA library was synthesised from high quality RNA (Agilent AffinityScript qPCR cDNA synthesis kit 600559) ...
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bioRxiv - Physiology 2023Quote: ... The resulting cDNA was profiled on a Bioanalyzer NanoChip (Agilent), then used as a template for library preparation according to the reagent kit protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... and cDNA integrity was assessed using D1000 ScreenTapes (Agilent Technologies). Libraries were pooled per species and each pool sequenced on Illumina NovaSeq6000 using either paired-end run sequencing 28 bp on read 1 and 94 bp on read 2 or 100 bp on each read.
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bioRxiv - Developmental Biology 2023Quote: The AffinityScript Multitemp cDNA Synthesis® kit (Agilent©, #200436) was used to convert polyA-mRNA to single-strand cDNA following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... and complementary DNA (cDNA) was reverse transcribed with Accuscript (Agilent) primed with a gene-specific oligonucleotide ...
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bioRxiv - Genetics 2024Quote: ... and cDNA integrity was assessed using D1000 ScreenTapes (Agilent Technologies). Libraries were pooled in an equimolar manner and 1000pM were paired-end sequenced on an Illumina NextSeq2000 (R1 = 30 cycles ...
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bioRxiv - Physiology 2024Quote: ... The resulting cDNA was amplifed in an AriaMX cycler (Agilent) with the ITaq Universal SYBR Green Supermix and ROX as reference dye (Bio-Rad ...
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bioRxiv - Genetics 2024Quote: ... The resulting cDNA was amplified in an AriaMX cycler (Agilent) with the ITaq Universal SYBR Green Supermix and ROX as reference dye (Bio-Rad ...
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bioRxiv - Cancer Biology 2020Quote: ... anti-IMP3 (1:1000, Cell Signaling 57145; 1:1000, DAKO clone 69.1), anti-mTOR (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... and anti-MHC-II (Dako 110843-002/Clone CR3/43, 1/400) in dilution buffer (1% goat serum ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibody for AR(Clone AR 441, DAKO, dilution at 1:75) was used with positive and negative controls run for each batch ...
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bioRxiv - Immunology 2024Quote: ... IHC staining against CD20 antigen (clone L26, 1:500 dilution; DAKO, Denmark) was performed using standard protocols for the Leica SM2000R automated stainer ...
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bioRxiv - Genomics 2024Quote: ... HLA-DR alpha-chain clone TAL.1B5 dilution 1:200 (M0746, Dako), CD68 clone PG-M1 dilution 1:100 (M0876 ...
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bioRxiv - Developmental Biology 2024Quote: ... Proliferating Cell Nuclear Antigen (PCNA, clone PC10, 1:200, Dako Cytomation, M0879), Myosin Heavy Chain (MF20 ...
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bioRxiv - Cell Biology 2020Quote: ... the quality and integrity of cDNA and cDNA libraries were quantitated using the High Sensitivity D5000 and D1000 ScreenTape↓ (Agilent# 5067-5592 and 5067-5584) on the Agilent-2200 TapeStation ...
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bioRxiv - Physiology 2022Quote: ... The purified RNA pellet was diluted in 50 µL RNase-free water and served as template for cDNA synthesis (AccuScript™ High Fidelity 1st Strand cDNA Synthesis; Agilent Technologies, Santa Clara, USA). PCR amplification was conducted using Phusion® DNA Polymerase (New England Biolabs ...
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bioRxiv - Cancer Biology 2020Quote: ... HRP-conjugated secondary antibodies used for detection were diluted 1:5000 (HRP-mouse anti rat, Cell Signaling Technology, Danvers, MA, Cat# CS7077S; HRP-mouse anti mouse, Dako, Agilent, Santa Clara ...
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bioRxiv - Cancer Biology 2020Quote: ... HRP-conjugated secondary antibodies used for detection were diluted 1:5000 (HRP-mouse anti rat, Cell Signaling Technology, Danvers, MA, Cat# CS7077S; HRP-mouse anti mouse, Dako, Agilent ...
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bioRxiv - Immunology 2020Quote: ... and isotype control: Glycans were prepared using the GlykoPrep® Rapid N-Glycan Preparation kit (PROzyme) and separated by Hydrophilic-Interaction Liquid Chromatography (HILIC ...
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bioRxiv - Immunology 2024Quote: ... Quality control was performed by estimating the concentration and measuring the average fragment size of the mRNA target library and sample tag library using the Agilent Tape Station with the High Sensitivity D1000 ScreenTape (Agilent, 5067-5584). The final libraries were diluted to a concentration of 4 nM and multiplexed for Novaseq paired-end sequencing (150 bp ...
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bioRxiv - Developmental Biology 2024Quote: Size distribution and yield quantification of the CUT&Tag libraries were tested using an Agilent 2100 Bioanalyzer high sensitivity DNA chip (Agilent 5067–4626). Libraries were then multiplexed and sequenced (paired-end 150 bp ...
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bioRxiv - Cell Biology 2021Quote: ... We performed exome sequencing in the child with neonatal diabetes and his consanguineous parents using the Agilent SureSelect Human All Exon Kit (Agilent SureSelect v4, 50Mb). We analyzed the data with our established pipeline in the institute (Kuhnen ...
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bioRxiv - Developmental Biology 2023Quote: ... We performed exome sequencing in the child with diabetes and his consanguineous parents and sibling using the Agilent SureSelect Human All Exon Kit (Agilent SureSelect v4, 50Mb) and next-generation sequencing (Hiseq ...
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bioRxiv - Developmental Biology 2021Quote: ... cDNA content and quality were assessed with D1000 (Agilent 5067-5582) or High sensitivity D1000 tape (Agilent 5067-5584 ...
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bioRxiv - Immunology 2021Quote: ... RNA and cDNA quality was analysed on a 2100 Bioanalyser (Agilent). mRNAseq libraries were prepared using Nextera XT DNA library preparation kit (Illumina ...
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bioRxiv - Neuroscience 2020Quote: ... JNK3 kinase dead (K55R mutant) cDNA was generated by Quickchange (Stratagene). mCherry-tagged JIP3 in FEW vector was as previously described (Holland et al ...