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Citations for Agilent :
601 - 638 of 638 citations for 3 Thiophen 3 yl benzoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2024Quote: ... The digestion was quenched with 10% formic acid and the resulting peptides were cleaned-up in an automated fashion using the AssayMap Bravo platform (Agilent Technologies) with a corresponding AssayMap C18 reverse-phase column ...
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bioRxiv - Cell Biology 2024Quote: ... were seeded at 6,000 cells per well in 80 µL of MEM media (MEM 10% FBS + 1% P/S + 1 mM Sodium Pyruvate + 2 mM Glutamax + 1% nonessential amino acids + 50 µg/mL uridine) in a Seahorse 96-well cell culture plate (Agilent, USA). After 24 hours ...
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bioRxiv - Cell Biology 2020Quote: ... acidified by nitric acid before the measurement of heavy metals by Inductively Coupled Plasma Optical Emission Spectrometer (Agilent ICP-OES 5110, America). The test methods were carried out according to standard protocol(Method of determination for 26 elements (copper ...
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bioRxiv - Evolutionary Biology 2021Quote: ... segetum to the synthetic mixture of C6-C10 straight chain acids were recorded on an Agilent 7890 gas chromatograph equipped with a flame ionization detector (FID) (Agilent Technologies, USA) and an electroantennographic detector (EAD ...
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bioRxiv - Bioengineering 2021Quote: ... pCS2-GemininΔ27-GFP was generated by deleting amino acids 1-27 from Geminin-GFP [27] using QuikChange® Lightning mutagenesis kit (Agilent, 210513). The plasmids pCS2-Flag-FA-Geminin-GFP w.t and Δ27 variant were generated by cloning Geminin-GFP into pCS2-Flag-FA vector sing FseI and AscI restriction sites ...
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bioRxiv - Systems Biology 2021Quote: ... detected with a diode array detector (DAD G7117A) and quantified following manufacturer’s guidelines (AdvanceBio Amino Acid Analysis, © Agilent Technologies, Inc. 2018). Standards ranging from 5 μM to 30 mM were used for the quantification of aspartate ...
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bioRxiv - Microbiology 2020Quote: Caprylic acid concentration was quantified by RP-HPLC following Herrera et al.37 using a liquid chromatographer (1220, Agilent Technologies; California, USA) equipped with an Eclipse XDB-C8 5 μm column (150 mm × 4.6 mm i.d.) ...
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bioRxiv - Cell Biology 2021Quote: ... The upper ether layer was collected and SCFA content was analyzed on an Agilent 7890B gas chromatography system with flame ionization detector using a high-resolution capillary column for detection of volatile acids (DB-FFAP, 30 m x 0.25 mm with 0.25 μm film thickness; Agilent Technologies, Santa Clara, CA). A standard solution containing 10 mM of acetic ...
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bioRxiv - Plant Biology 2020Quote: ... the HY5 36th Serine (AGC) was changed into Alanine (GCC) and Aspartic Acid (GAC) respectively using Quickchange II site-directed mutagenesis kit (Agilent, Catalog #200523) and cloned into pB7FWG vectors ...
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bioRxiv - Microbiology 2020Quote: Each ELV and MV preparation were reconstituted in 10 μl of 5% formic acid and injected onto a 50 mm 300 μm C18 trap column (Agilent Technologies, USA). The samples were then desalted for 5 min at 30 μl/min using 0.1% formic acid and the peptides were then eluted onto an analytical nano-HPLC column (150 mm × 75 μm 300SBC18 ...
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bioRxiv - Cancer Biology 2020Quote: ... The concentration of total RNA was measured on a Nanodrop device and the quality of the extracted nucleic acid was assessed using a Bio-analyzer 2100 (Agilent technologies, CA). Microarray probes were synthetized in one cycle of RNA amplification in which molecules were labeled (Affymetrix microarray station ...
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bioRxiv - Cancer Biology 2020Quote: ... Concentration of total RNA was measured using a Nanodrop spectrophotometer and the quality of the extracted nucleic acid was assessed on a Bio-analyzer 2100 (Agilent Technologies, CA). Microarray probes was synthetized by one cycle of RNA amplification in which molecules were labeled in an Affymetrix microarray station (Affymetrix ...
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bioRxiv - Systems Biology 2022Quote: ... the RNA integrity number was 10 as assessed by the Stanford Protein and Nucleic Acid (PAN) Biotechnology Facility using the RNA Nano Kit (Agilent #5067-1511) on an Agilent Bioanalyzer ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Amino acid substitutions or deletions were introduced into the pSARS-CoV-2-SΔ19 expression vector by site-directed mutagenesis (Stratagene, La Jolla, CA) by following the manufacturer’s instructions and using mutagenic oligonucleotides SaCoV2-K417T-F ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were resuspended in hexane and fatty acids were separated with gas chromatography (8860 or 7890A GC system, Agilent Technologies, CA, USA) combined with mass spectrometry (5977B or 5975C Inert MS system ...
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bioRxiv - Physiology 2023Quote: ... High-resolution gas chromatography capillary column coated with 0.25µm film thickness was used (DB-FFAP) for the detection of volatile acids (Agilent Technologies, Santa Clara, CA). The oven temperature was 145°C and the FID and injection port were set to 225°C ...
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bioRxiv - Microbiology 2023Quote: ... Elemental quantification on acid-digested liquid samples was performed using an Agilent 7700 inductively coupled plasma mass spectrometer (Agilent, Santa Clara, CA). The following settings were fixed for the analysis Cell Entrance = −40 V ...
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bioRxiv - Cancer Biology 2022Quote: ... RAS double mutants with point-mutations in the 95th and 96th amino acid residues were generated by site-directed mutagenesis (Agilent Technologies #200521) using the primers listed in Supplementary Table 2 per manufacturer’s recommended PCR conditions ...
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bioRxiv - Biophysics 2024Quote: ... that is loaded onto a Waters HDX-Manager with a desalting flow of 0.23% aqueous formic acid solution (Solvent A) provided by an Agilent 1260 Infinity quaternary pump (Agilent Technologies, CA, USA) and a gradient flow comprised of Solvent A (0.23% aqueous FA solution ...
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bioRxiv - Microbiology 2024Quote: ... Elemental quantification on acid-digested liquid samples was performed using an Agilent 7700 inductively coupled plasma mass spectrometer (Agilent, Santa Clara, CA). The following settings were fixed for the analysis Cell Entrance = −40 V ...
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bioRxiv - Cell Biology 2024Quote: ... We mutated the Serine 69 to Alanine (69A) or aspartic acid (69D) using the QuickChange II Site-directed Mutagenesis kit (Agilent Technologies, #200523). The primer for mutating S69-A was ...
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bioRxiv - Pathology 2020Quote: ... resuspended to 0.5 µg/µL in 90% ACN/0.1% trifluoroacetic acid (TFA) and fractionated with an Agilent 1200 high performance liquid chromatography (HPLC) system (Agilent Technologies, Santa Clara, CA) on an in-house 17 cm TSKgel Amide-80 HILIC column (3 µm particle size ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Amino acid substitutions or deletions were introduced into the pSARS-CoV-2-Strunc expression vector by site-directed mutagenesis (Stratagene, La Jolla, CA) using mutagenic oligonucleotides as follow:
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bioRxiv - Immunology 2022Quote: FFPE tissue sections were deparaffinized and antigen retrieval was undertaken in 0.1 M citric acid buffer solution (Dako, target retrieval solution, S169984-2) using an electric pressure cooker (Russell Hobbs RHNHP401) ...
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bioRxiv - Physiology 2021Quote: ... Amino acid substitution mutants were generated by site-directed mutagenesis using a QuickChange site-directed mutagenesis kit (Agilent Technologies, La Jolla, CA, USA). The predicted DNA sequences of all constructs were verified by sequencing.
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bioRxiv - Cancer Biology 2023Quote: ... The oxygen consumption rate (OCR) assay was performed using the XF long-chain fatty acid oxidation (LCFA) stress test kit (Agilent, cat# 103672-100) and Seahorse XFe24 Analyzer was used to investigate the long-chain fatty acid OCR according to the manufacturer’s protocol.
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bioRxiv - Bioengineering 2023Quote: ... An aliquot of 1 mL was obtained and diluted with 2% nitric acid before running for elemental analysis using an inductively coupled plasma optical emission spectrometer (ICP-OES, Varian 720-ES, Agilent, Santa Clara, CA). For each run ...
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bioRxiv - Microbiology 2021Quote: ... and branched-chain amino acids (BCAAs) was performed with an Agilent 7890A gas chromatograph coupled with an Agilent 5975 mass spectrometer (Agilent Technologies Santa Clara, CA) using a propyl esterification method as previously described [61] ...
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bioRxiv - Immunology 2022Quote: ... Reactions were stopped by adding 50 μL 3M hydrochloric acid and absorbance at 492 nm was determined on a Synergy 4 plate reader (BioTek, Agilent Technologies inc., CA, USA) or similar ...
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bioRxiv - Biochemistry 2021Quote: ... Peptides were loaded with solvent A (0.1% formic acid in water) onto an in-house packed analytical column filled with Poroshell EC120 C18 material (Agilent Technologies, Santa Clara, CA, USA). Peptides were chromatographically separated at a constant flow rate of 250 nL/min using the following gradient ...
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bioRxiv - Physiology 2024Quote: ... Etomoxir (an inhibitor of long chain fatty acid oxidation) provided in the Seahorse XF Mito fuel flex test kit (Agilent Technologies, Cat#103260-100). Fuel dependency for a specific pathway was measured after inhibiting this pathway ...
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bioRxiv - Biochemistry 2022Quote: ... system coupled to an Agilent 6470 series QQQ mass spectrometer (MS/MS) was used to analyze fatty acids in each sample (Agilent Technologies, Santa Clara, CA). The methods are similar to what is described in the paper by Yang ...
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bioRxiv - Genomics 2024Quote: ... The measurement of the mineral nutrients in the acid digests was conducted by inductively coupled plasma optical emission spectroscopy (ICP-OES; Agilent 5110 Vertical Dual View). The measurements were verified by using certified standard reference material (SRM ...
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bioRxiv - Immunology 2024Quote: ... The reaction was stopped with trifluoroacetic acid, and peptides were analyzed by RP-HPLC (Shimatzu, Kyoto, Japan) on an AdvanceBio Peptide Plus C18 column (Agilent Technologies, Santa Clara, CA). The samples (100 µL ...
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bioRxiv - Pathology 2024Quote: Twenty to fifty grams of frozen caecal contents were used to analyzed the short-chain fatty acids (SCFAs) composition using gas chromatography (Agilent 7890B gas chromatograph, Agilent Technologies, Les Ulis, France) as previously described (25).
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bioRxiv - Microbiology 2024Quote: ... Two amino acid substitutions (S299A and T309A) were carried out using specific primers (Table S1) and Pfu Turbo Polymerase (Agilent Technologies, Santa Clara, CA, USA). Then amplicons were further treated with DpnI (New England BioLabs ...
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bioRxiv - Cell Biology 2022Quote: ... 2cm x 100µM) for 5min in solvent A (0.1% formic acid in water) before being separated on an analytical column (Agilent Poroshell, EC-C18, 2.7µM, 50cm x 75µM). Solvent B consisted of 0.1% formic acid in 80% acetonitrile ...
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Spatiotemporal proteomics reveals the biosynthetic lysosomal membrane protein interactome in neuronsbioRxiv - Cell Biology 2024Quote: ... with solvent A (0.1% formic acid in water) at 30 µl/min flowrate and chromatographically separated over the analytical column (Poroshell 120 EC C18, Agilent Technologies, 50 μm x 75 cm, 2.7 μm) using 180 min gradient at 300 nL/min flow rate ...