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Citations for Agilent :
501 - 550 of 638 citations for 3 Thiophen 3 yl benzoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Single amino acid substitutions of GLIC were engineered using the QuikChange II Site-Directed Mutagenesis kit (Agilent Technologies) and oligonucleotide primers (Sigma) ...
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bioRxiv - Biochemistry 2022Quote: ... 0.3 μL of acetic acid was added to the sample before desalting with a C18 tip (Agilent A57203). The C18 tip was washed three times with 200 μL of methanol ...
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bioRxiv - Plant Biology 2023Quote: ... The chromatographic separations of free amino acids were carried out using a HILIC-Z column (2.1×150 mm, particle size 2.7 µm, Agilent) employing a linear binary gradient of (A ...
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bioRxiv - Plant Biology 2024Quote: ... Oligosaccharides were reductively aminated with 2-aminobenzoic acid (2-AA) and cleaned using a GlycoClean S cartridge (ProZyme) as previously described (Tryfona & Stephens ...
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bioRxiv - Immunology 2024Quote: ... Eluted tryptic peptides were acidified with 1% v/v formic acid and desalted with C18 Omix tips (Agilent).
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bioRxiv - Plant Biology 2024Quote: Methyl ester derivatives of total fatty acids (FAMEs) were analysed by Gas Chromatography (GC) (Agilent 7890A, Agilent Technologies) using an Agilent J&W 122-2332 column (30 m × 250 µm × 0.25 µm ...
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bioRxiv - Plant Biology 2024Quote: Methyl ester derivatives of total fatty acids (FAMEs) were analysed by Gas Chromatography (GC) (Agilent 7890A, Agilent Technologies) using an Agilent J&W 122-2332 column (30 m × 250 µm × 0.25 µm ...
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bioRxiv - Biochemistry 2024Quote: ... Automated dilution was performed using 5 μL of solvent A (99.9% water + 0.1% formic acid) with the Bravo liquid handler (Agilent). Finally ...
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bioRxiv - Biochemistry 2024Quote: ... samples were diluted to 1 mg/mL in 0.2% formic acid and injected via HPLC (Agilent 1100 HPLC) and eluted in a mobile phase of formic acid/methanol ...
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bioRxiv - Systems Biology 2020Quote: ... A high resolution gas chromatography capillary column 30m x 0.25 mm coated with 0.25 μm film thickness was used (DB-FFAP) for the volatile acids (Agilent Technologies) and a high resolution gas chromatography capillary column 30m x 0.25 mm coated with 0.50 μm film thickness was used (DB-FFAP ...
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bioRxiv - Biochemistry 2021Quote: ... Selected residues were mutated to alanine or oppositely-charged amino acids by PfuUltra II Hotstart PCR Master Mix (Agilent). All mutated sequences were confirmed by Sanger sequencing ...
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bioRxiv - Biochemistry 2020Quote: ... Each reaction was quenched by 30 x dilution into 0.1% formic acid and loaded onto an electrospray ionisation time-of-flight (LC-MSD TOF) spectrometer (Agilent).
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bioRxiv - Microbiology 2020Quote: ... The codon at amino acid position 160 in HA (H3 numbering, Threonine) was modified via site-directed mutagenesis (Agilent) from the wild type (ACA ...
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bioRxiv - Microbiology 2022Quote: ... Amino acid substitutions and deletions were introduced into pET28b vectors with the QuickChange II Site-Directed mutagenesis kit (Stratagene), according to the manufacturer’s directions ...
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bioRxiv - Plant Biology 2021Quote: ... and 2% Buffer B (0.1% formic acid in Optima grade acetonitrile (Fisher)) using a 1200 series capillary pump (Agilent). Following loading ...
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bioRxiv - Systems Biology 2023Quote: ... the supernatant was diluted with mobile phase (10mM Ammonium acetate 0.03% formic acid) and filtered using a 0.45 µm 96-well microplate (polypropylene membrane, Cat. No. 200983-100, Agilent) by centrifugation for 30 minutes at 5,250 x g ...
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bioRxiv - Pathology 2023Quote: Sugars and Organic acids were quantified in the RCJ using high-performance liquid chromatography (HPLC) (1200 series, Agilent Technologies) with a diode-array detector (DAD ...
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bioRxiv - Biochemistry 2024Quote: ... linear gradient from H2O/MeCN + 0.1 trifluoracetic acid from 10 to 90% over 60 min) on a 1260 Infinity II LC System (Agilent). Peaks were collected manually according to the peak height at 480 nm ...
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bioRxiv - Neuroscience 2024Quote: The fatty acid profile and content in 10 mg of each fecal sample were determined by gas chromatography (Agilent Technologies 6890 N Series Gas Chromatograph ...
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bioRxiv - Microbiology 2022Quote: ... Crude peptide was subsequently purified by reverse phase high-pressure liquid chromatography on a C18 semiprep column over an acetonitrile gradient with 0.1% trifluoroacetic acid and analyzed by liquid chromatography triple quadrupole mass spectrometry (Agilent).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... then reconstituted in 1 mL of 0.1 % trifluoroacetic acid and desalted using an Agilent Macroporous Reversed-Phase C18 column (4.6 × 50 mm mRP-C18, Agilent) on a 1260 Infinity HPLC system (Agilent).
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bioRxiv - Developmental Biology 2023Quote: ... Periodic acid-Schiff (PAS) and hematoxylin and eosin (H&E) staining were performed using standard procedures (Dako, Glostrup, Denmark) for the assessment of normal structures.
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bioRxiv - Microbiology 2024Quote: ... Cell free supernatants were acidified with formic acid (0.1% final concentration) and analysed by GC-FID using a DB-FFAP column (Agilent).
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bioRxiv - Plant Biology 2024Quote: ... Separation of amino acid was done with a Zorbax Eclipse XDB-C18 column (50 × 4.6 mm, 1.8 μm, Agilent Technologies). The mobile phase comprised of solvent A (water ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Amino acid deletions for ΔKPQ were generated by use of the QuikChange Lightning Site-Directed Mutagenesis kit (Agilent, USA). PCR products were cloned into XL-10 Gold Ultracompetent cells ...
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bioRxiv - Physiology 2024Quote: ... Peptides were reconstituted in 50 µL 15% ACN/0.1% acetic acid and analyzed on an 1290 Infinity II LC system (Agilent) coupled to QTRAP instrument (Sciex ...
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bioRxiv - Cell Biology 2024Quote: ... All point mutations and amino acid deletions were incorporated into constructs using the QuikChange site-directed mutagenesis kit (Agilent). All constructs were expressed using either the pINDUCER20-C1-EGFP lentiviral vector (Crawley et al. ...
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bioRxiv - Microbiology 2020Quote: ... The 75th amino acid in the PB1-F2 protein was changed to a histidine (H) via site directed mutagenesis (QuikChange Lightning, Agilent) to produce the PB1-F2-75H plasmid ...
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bioRxiv - Plant Biology 2020Quote: ... and fluorenylmethoxycarbonyl (FMOC) was based on the application note “Automated amino acids analysis using an Agilent Poroshell HPH-C18 Column” by Agilent. The samples were injected onto a 100 mm x 3 mm InfinityLab Poroshell HPH-C18 column (2.7 μm ...
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bioRxiv - Cell Biology 2020Quote: Samples from all biological replicates were first sent to the Stanford University Protein and Nucleic Acid Facility (Stanford, CA) for quantification and quality analysis using a 2100 Bioanalyzer (Agilent). Samples were then sent to Novogene Corporation Inc ...
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bioRxiv - Microbiology 2021Quote: ... Fatty acid methyl esters were identified by their mass spectrum and retention time and quantified by Mass Hunter Quantification Software (Agilent) and the calibration curve generated with fatty acid methyl esters standards mix (Sigma CRM47885) ...
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bioRxiv - Biochemistry 2020Quote: ... The D614G amino acid change was introduced into VRC7480 by site-directed mutagenesis using the QuikChange Lightning Site-Directed Mutagenesis Kit from Agilent Technologies (Catalog # 210518) ...
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bioRxiv - Immunology 2020Quote: ... The codon encoding residue 241 of Copa was mutated from glutamic acid to lysine via Quikchange Lightning site directed mutagenesis (Agilent). Following NotI and PacI digestion ...
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bioRxiv - Microbiology 2020Quote: ... Hydrolyzed amino acids were separated using ultra performance liquid chromatography (UPLC, Acquity, Waters) on a C-8 column (Zorbax Eclipse XBD, Agilent) at a flow rate of 0.6 mL/min ...
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bioRxiv - Biochemistry 2020Quote: E.coli expression plasmids encoding GST-PEX14-NTD with amino acid substitutions were constructed via Site Directed Mutagenesis using QuikChange II (Stratagene). ß-tubulin (amino acids 388–444 ...
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bioRxiv - Bioengineering 2020Quote: ... rehydrated slides were subject to antigen retrieval using citric acid buffer at 120°C and 20 psi in a pressure cooker (DAKO). Afterwards ...
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bioRxiv - Biophysics 2020Quote: ... Chromatographic separation of OPA-derivatized amino acids was performed using an Agilent ZORBAX Eclipse AAA column (4.6mm x 150 mm x 3.5 μm; Agilent #963400-902) coupled with a ZORBAX Eclipse AAA Analytical Guard Column (4.6 mm x 12.5 mm x 5 μm ...
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bioRxiv - Microbiology 2021Quote: Levels of volatile fatty acids present in the supernatant of both co-cultures and monocultures were measured using an Agilent1260 Infinity HPLC (Agilent). Samples were prepared by acidifying to 5 mM using sulfuric acid and subsequently incubating at room temperature for 5 minutes ...
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bioRxiv - Microbiology 2020Quote: ... The 13C-labeling patterns of proteinogenic amino acids were determined on a 6890N Network GC system with a 5975 inert XL mass selective detector (Agilent Technologies Inc. ...
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bioRxiv - Immunology 2022Quote: ... The D614G amino acid change was introduced into VRC7480 by site-directed mutagenesis using the QuikChange Lightning Site-Directed Mutagenesis Kit from Agilent Technologies (catalog no ...
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bioRxiv - Immunology 2021Quote: ... we changed three amino acids within the Fc region by using the QuikChange II site directed mutagenesis kits (Agilent, #200523), introducing M257Y ...
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bioRxiv - Neuroscience 2021Quote: ... TDP-43N-Del was generated by deletion of the first 81 amino acids from the N-terminus of TDP-43WT using the QuickChange Site-Directed Mutagenesis Kit (Agilent).
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bioRxiv - Molecular Biology 2020Quote: The conservative lysine at position 23 was replaced with glutamic acid to create Meier-Gorlin mutation (K23E) following site-directed mutagenesis protocol (Agilent). Drosophila-Human and Human-Drosophila hybrids were designed by using a PCR technique ...
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bioRxiv - Plant Biology 2021Quote: Salicylic acid (SA) was extracted from frozen leaf material and analyzed by ultra-high-performance liquid chromatography system (Agilent Technologies) coupled to an Agilent 6530 quadrupole time of flight-mass spectrometer (Agilent Technologies ...
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bioRxiv - Cell Biology 2020Quote: The mCherry-TRAK1 deletion mutant mCherry-TRAK1Δ was obtained by inserting a stop codon after amino acid 635 of the mCherry-TRAK1 encoding nucleotide sequence by means of a PCR-based mutagenesis (Agilent Technologies ...
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bioRxiv - Microbiology 2021Quote: ... The extracted phospholipids were methanolized as fatty-acid methyl esters and then analysed using gas chromatography (Agilent Technologies 6890N, UK) [26] ...
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bioRxiv - Microbiology 2020Quote: ... The GFP-tagged N127143Q mutant was constructed by substitution of asparagines for glutamines at amino acids 127 and 143 by site-directed mutagenesis (Stratagene). The GFP-Rem-T7 partial deletion mutants (GFP-RemΔ103-155-T7 ...
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bioRxiv - Microbiology 2023Quote: The substitution of amino acids via SDM was carried out using the QuikChange II Site-Directed Mutagenesis Kit (Agilent Technologies) per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Binding of VHHs to spike proteins on the surface of mammalian cells was determined by flow cytometry using pcG1-expression plasmids containing the coding sequence of the SARS-CoV-2 spike protein from which the C-terminal 18 amino acids were deleted and in which the D614G substitution was introduced by QuickChange site-directed mutagenesis (Agilent) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... stopped with Stop Solution (acid) and absorbance values were recorded at 450 nm on a spectrophotometer (using a BioTeck Gen5 plate reader (Agilent).