Labshake search
Citations for Agilent :
6351 - 6400 of 8085 citations for Rat Nuclear Factor Of Activated T Cells 5 NFAT5 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... Sequencing libraries were validated using the NGS Kit on the Agilent 5300 Fragment Analyzer (Agilent Technologies, Palo Alto, CA, USA), and quantified with the Qubit 4.0 Fluorometer (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was isolated by RNeasy Mini or RNeasy Plus Micro Kit and controlled for quality with a 2100 Bioanalyzer (Agilent) prior to cDNA libraries construction ...
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bioRxiv - Neuroscience 2023Quote: ... Transcribed cDNA was diluted ten-fold and used for real-time quantitative PCR (QuantiTect SYBR Green PCR Kit; Quiagen; 204145) performed with the MX3005P thermocycler (Stratagene).
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bioRxiv - Molecular Biology 2023Quote: ... mutant plasmids encoding the different RNA exosomopathy amino acid substitutions were generated by site-directed mutagenesis of the respective wild-type plasmids (pAC3656, pAC3652, pAC3482) using the QuikChange II Site-Directed Mutagenesis Kit (Agilent) and oligonucleotides containing the desired missense mutations as previously described [33 ...
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bioRxiv - Genomics 2023Quote: ... the concentration and integrity of the RNA were assessed using the ND-1000 UV-visible light spectrophotometer from Nanodrop Technologies and the Bioanalyzer 2100 with the RNA 6000 Nano Lab Chip kit by Agilent.
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bioRxiv - Genetics 2023Quote: ... The quality and concentration of the library were assessed using the Femto Pulse Genomic DNA 165 kb Kit (Agilent Technologies) and Qubit dsDNA HS Assay (ThermoFisher) ...
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bioRxiv - Genomics 2023Quote: ... The integrity of the RNA sample was verified using the Agilent RNA 6000 kit and Agilent Bioanalyzer 2100 (Agilent Technologies). Samples with an RNA Integrity Number (RIN ...
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bioRxiv - Immunology 2023Quote: ... Specific nucleotide substitutions or coding-sequence truncations were introduced as required using the QuikChange XL Site-Directed Mutagenesis Kit (Agilent). All plasmid inserts and modifications were authenticated by Sanger sequencing.
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bioRxiv - Genomics 2023Quote: ... and resulting size distribution was assessed with the High Sensitivity (HS) Large fragment kit run on the Fragment Analyzer (Agilent Technologies ...
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bioRxiv - Microbiology 2023Quote: ... The integrity of the RNA was tested on a Bioanalyzer 2100 using the Prokaryote Total RNA Nano kit (Agilent Technologies) and RNA Integrity Numbers (RIN ...
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bioRxiv - Developmental Biology 2023Quote: ... Libraries were analysed on the 2100 Bioanalyzer Agilent system using the Agilent High Sensitivity DNA kit (Agilent Technologies, 5067-4626). DNA concentrations were measured by Qubit Quant IT kit (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... Purified pools were quantified using Qubit dsDNA HS Assay and the quality checked using a Tapestation High Sensitivity D1000 kit (Agilent) to ensure the size distribution of amplicons was as expected ...
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bioRxiv - Cell Biology 2023Quote: ... RNA was quantified by NanoDrop and loaded abundance measured using a Pico 6000 kit and Eukaryotic Total RNA assay on a BioAnalyzer (Agilent).
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bioRxiv - Cancer Biology 2023Quote: ... The size distribution and quality of the libraries were evaluated using a high sensitivity DNA kit (Agilent Technologies #5067-4626). Quantification of libraries was carried out with the Quant-iT DNA Assay Kit ...
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bioRxiv - Cell Biology 2023Quote: ... the codon for C34 in pCEP4-MFAP4 was modified from TGC to TCG using the QuickChange Site Directed Mutagenesis kit as instructed by the manufacturer (Agilent). The entire insert in pCEP4-MFAP4 and the sequence coding for the C34S mutation in pCEP4- MFAP4C34S was validated by Sanger DNA sequencing ...
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bioRxiv - Cell Biology 2023Quote: ... The mutations corresponding to the MARylation sites of RACK1 (D144N/E145Q/D203N) were introduced into the pCMV3-RACK1 plasmid using the QuikChange Site-Directed Mutagenesis kit (Agilent).
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bioRxiv - Bioengineering 2023Quote: ... The library of CjCas9 variants was produced by random mutagenesis of the wild-type sequence using error-prone PCR approach (GeneMorph II kit from Agilent) (for primers ...
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bioRxiv - Biochemistry 2023Quote: ... The NopD point mutants constructs were generated by different primers and were created by the QuickChange site-directed mutagenesis kit (Stratagene). All primers are shown in the Supplementary Table1 ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA-seq libraries were prepared using the QuantSeq 3′ mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen) and assessed on a BioAnalyzer 2100 (Agilent) for library quantification and quality control ...
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bioRxiv - Genomics 2023Quote: ... Final library concentration was assessed by Qubit dsDNA HS Assay Kit (Thermo-Fischer Scientific) and post library QC was performed using Tapestation High Sensitivity D1000 (Agilent) to ensure that fragment sizes were distributed as expected ...
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bioRxiv - Bioengineering 2023Quote: ... Quality assessment of the isolated RNAs from each condition was conducted using the RNA 6000 Pico Kit and the 2100 Bioanalyzer Systems (Agilent), while quantification was carried out using the Qubit system (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... vector containing the SARS-CoV-2 HA-ORF3a gene was used in site-directed mutagenesis using a QuikChange II site-directed mutagenesis kit (Agilent) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... and quality checked using the Qubit dsDNA HS Assay Kit (Thermo cat. #Q32854) and a Bioanalyzer 2100 (Agilent cat. # G2939A) High Sensitivity DNA Kit (Agilent cat ...
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bioRxiv - Immunology 2024Quote: ... From this construct, CDC42 point mutants (R186C, C188Y, Y64C) were obtained by site-directed mutagenesis (Quick change kit, Agilent Technologies). The *192C*24 plasmid was produced by ThermoFisher.
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bioRxiv - Genomics 2024Quote: ... cDNA libraries were quantified using the Qubit dsDNA Assay Kit (Thermo, Q32851) and library quality was assessed with the 4150 Tapestation System (Agilent) and D5000 screen tapes (Agilent ...
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bioRxiv - Cell Biology 2024Quote: ... The library profiles were checked using High Sensitivity NGS Fragment Analysis kit (1-6000bp) (Agilent Technologies, reference DNF-474-1000) on the 5300 Fragment Analyzer System ...
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bioRxiv - Cell Biology 2024Quote: ... Qualitative and quantitative measurement of cDNA was conducted with the High Sensitivity NGS Fragment 1-6000bp kit on a 48-channel Fragment Analyzer (Agilent) and 1x dsDNA kit on the Qubit 4 Fluorometer (ThermoFisher) ...
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bioRxiv - Genetics 2024Quote: ... The BP-lowering rs1173771 haplotype was then constructed by site directed mutagenesis at the 11 SNPs locations in the haplotype using QuikChange II Site-directed mutagenesis kit (Agilent) and confirmed by Sanger sequencing ...
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bioRxiv - Immunology 2024Quote: ... The glycolytic function of infected macrophage was analyzed by the Agilent Seahorse XF Glycolytic Stress Test Kit (Agilent, 103020-100). Mφs and amastigotes were distributed in Seahorse XF96 tissue culture microplates for 3 days at 34°C ...
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bioRxiv - Biochemistry 2024Quote: The plasmid for expression of the rare codon reporter pCIneo-RLuc (30xRC) was generated by site-directed mutagenesis using the QuikChange Site-Directed Mutagenesis kit (Stratagene). Protein mutant plasmids used in this study are listed in Supplementary Table S1 ...
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bioRxiv - Genomics 2024Quote: ... RNA integrity (RIN) values for each sample were determined using the Agilent RNA 6000 Nano chip kit (Agilent, 5067-1511) and an Agilent Bioanalyzer 2100 instrument following the manufacturer’s protocol.
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bioRxiv - Developmental Biology 2024Quote: ... and the mean fragment size was assessed using the DNA High Sensitivity KIT on a 2100 Bioanalyzer (Agilent Technologies, Netherlands). 4 nM pooled libraries was sequenced on NovaSeq S1 flow cell by Livestock Improvement Corporation Ltd ...
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bioRxiv - Biochemistry 2024Quote: ... were introduced into all TE expression plasmids in place of codons for the catalytic Ser or Cys residues using the QuikChange XL Site-Directed Mutagenesis kit (Agilent), optimized for GC-rich sequences (JuvEV TE S1637amb ...
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bioRxiv - Neuroscience 2024Quote: ... Site-directed mutagenesis was either provided by VectorBuilder or performed similar to the Quik-Change Site-Directed mutagenesis kit from Agilent Technologies (Santa Clara ...
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bioRxiv - Plant Biology 2024Quote: ... Quality and quantity of the finished libraries were assessed using an Agilent DNA 1000 kit (Agilent Technologies, Santa Clara, CA) and Qubit® dsDNA HS Assay Kit (ThermoFisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... The quality control and the fragment size distribution were determined using the DNF-474 High sensitivity NGS kit on a Fragment analyzer (Agilent).
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bioRxiv - Cell Biology 2024Quote: ... were designed to replace the relevant amino acid residue with alanine and carried out using the PfuUltra II Fusion HS DNA Polymerase kit (600670, Agilent) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... The average size of the library was determined by the Agilent High Sensitivity DNA Kit (Agilent, Santa Clara, CA, USA). An accurate library quantification was determined using the Library Quantification Kit – Illumina/Universal Kit (KAPA Biosystems ...
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bioRxiv - Microbiology 2024Quote: ... RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA), and then sequenced on TruSeq PE Cluster Kit v3-cBot-HS (Illumina ...
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bioRxiv - Microbiology 2024Quote: The site-directed mutants of PtPelA and PaPelA without its predicted signal sequence (amino acids 47-948 for PaPelA; accession Q9HZE4) were generated using the QuikChange Lightning site-directed mutagenesis kit (Stratagene). The sequence of all vectors was confirmed using DNA sequencing.
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bioRxiv - Microbiology 2024Quote: ... TamA G271C and G574C substitutions were introduced into pXW48 using the QuikChange Site-Drected Mutagenesis Kit (Agilent catalog number 200524). Plasmids that encode bamABCDE8His-bamB (pYG120 ...
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bioRxiv - Cell Biology 2024Quote: ... RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). RNA-seq library construction was performed as described previously (Xu et al. ...
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bioRxiv - Immunology 2024Quote: ... RNA was quantified by NanoDrop (Thermo-Fisher) and size distribution was analyzed by using Agilent RNA 6000 Nano kit (Agilent).
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bioRxiv - Microbiology 2024Quote: ... The integrity of the RNAs was verified using the Agilent 2100 bioanalyzer with the RNA 6000 Nano kit (Agilent Technologies). qRT-PCR experiments and data analysis were performed as described (38) ...
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bioRxiv - Microbiology 2024Quote: ... The PCR amplicons were subsequently separated by Fragment Analyzer capillary electrophoresis using the DNF905 1-500 base pair kit (Agilent). Bands were quantified and PSI was calculated as follows ...
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bioRxiv - Molecular Biology 2024Quote: ... One hundred twenty-five ng of each sample was pooled prior to quality control and quantification on the 2100 Bioanalyzer system using DNA 7500 kit (Agilent) and KAPA Library Quantification Kit Illumina/Universal (Roche) ...
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bioRxiv - Molecular Biology 2024Quote: ... The resulting libraries tagged with unique dual indices were checked for size and quality using the Agilent High Sensitivity DNA Kit (Agilent). Library concentrations were measured using the KAPA SYBR Fast qPCR kit (Roche ...
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bioRxiv - Evolutionary Biology 2024Quote: ... from these samples (muscle or blood) using Qiagen MagAttract HMW DNA Kit and checked the DNA quality using TapeStation (Agilent), Qubit ...
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bioRxiv - Developmental Biology 2024Quote: Site-directed mutagenesis of Branchiostoma floridae Pax6 cDNA cloned in pKW mammalian expression vector was performed by the Quick-Change kit (Stratagene) using primers zk2027A/zk2027B to generate Pax6ΔQL ...
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bioRxiv - Plant Biology 2024Quote: ... The quality of the adapter-ligated libraries was checked on the BioAnalyzer High Sensitivity DNA kit (Cat no: 5067-4626. Agilent) or Caliper GX LabChip GX/HT DNA high sensitivity kit (Cat no ...