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Citations for Agilent :
6201 - 6250 of 7968 citations for Mouse Antigen peptide transporter 2 TAP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... The resulting PCR products were subcloned into the holding vector pSC-B (StrataClone Blunt PCR Cloning Kit, Agilent Technologies) and 16 colonies (white ...
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bioRxiv - Biochemistry 2023Quote: ... For generation of single point LIR mutants and ΔLIR BAG3 the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent, 210518) was utilized according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... Concentration and quality of the synthesised cDNA were assessed using the Bioanalyzer High-Sensitivity DNA Kit (Agilent, 5067-4627). cDNA was successfully amplified from the suspension containing 8,000 nuclei however cDNA prepared from the suspension containing 8,000 cells was undetectable and therefore library preparation was discontinued at this step ...
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bioRxiv - Cell Biology 2023Quote: ... and RNA integrity was checked with RNA Kit on Agilent 5300 Fragment Analyzer (Agilent Technologies, Palo Alto, CA, USA). RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina following the manufacturer’s instructions (NEB ...
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bioRxiv - Cell Biology 2023Quote: ... Sequencing libraries were validated using NGS Kit on the Agilent 5300 Fragment Analyzer (Agilent Technologies, Palo Alto, CA, USA), and quantified by using Qubit 4.0 Fluorometer (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... 2.2 kb) 20 was labelled by [a-32P]-dCTP by Prime-It II Random Primer Labeling Kit (Agilent Technologies) and hybridized with Hybond-N ...
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bioRxiv - Cell Biology 2023Quote: Single point mutations were inserted in the human RAB6A sequence at position 156 using the QuickChange mutagenesis kit (Agilent).
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bioRxiv - Immunology 2023Quote: ... SAMD9L-F886Lfs*11-E198A/D243A were generated from pMAX:mTFP1-SAMD9L plasmid using the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... λgt10 phage vectors in genomic DNA were excised using commercial packaging extract kits (Agilent Technology, Santa Clara, CA, USA), according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... and fragment size distribution with the Agilent® High Sensitivity DNA Kit (Agilent Technologies, Inc., Cat. No. 5067-4626). Libraries were sequenced as paired-end 150×150bp on a NovaSeq® 6000 System (Illumina ...
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bioRxiv - Genomics 2023Quote: ... and quality was determined with a DNA ScreenTape Kit on the TapeStation 2200 system (Agilent; 5067-5582, 5067-5583). Sequencing was done using a HiSeq 2500 (Illumina).
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bioRxiv - Cell Biology 2023Quote: ... Amplified cDNA and final libraries were evaluated on an Agilent BioAnalyzer using a High Sensitivity DNA Kit (Agilent Technologies). Individual libraries were diluted to 4nM and pooled for sequencing ...
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bioRxiv - Developmental Biology 2023Quote: ... PCR amplicons were further cloned into a pSC-amp/kan vector using StrataClone PCR cloning kit (240205, Agilent Technologies). We picked ∼20-50 single colonies per sample to determine the presence ...
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bioRxiv - Genomics 2023Quote: ... and size distribution was determined using the high sensitivity DNA kit on the Bioanalyzer 2100 (Agilent, Santa Clara, CA). Pooled libraries were sequenced on the Illumina NovaSeq (paired-end 150 ...
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bioRxiv - Biochemistry 2022Quote: AtCOSY mutants were generated according to the protocol described in the QuickChange II Site-Directed Mutagenesis Kit (Agilent Technologies) using plasmid pHis8-4b::AtCOSY as the template and the primer sequences in Supplementary Table 5 ...
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bioRxiv - Neuroscience 2023Quote: ... RNA quality was assessed using an Agilent 2100 bio-analyzer and the RNA 6000 pico kit (Agilent 5067-1513). Next ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The quantity and quality of extracted RNA samples were analyzed using RNA 6000 Nano kit on a bioanalyzer (Agilent). RNA samples with RNA integrity number (RIN)>7 were used for library construction using the 3′ mRNA-Seq Library Prep Kit FWD for Illumina (QuantSeq-LEXOGEN ...
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bioRxiv - Cell Biology 2023Quote: ... Sequencing libraries were validated using NGS Kit on the Agilent 5300 Fragment Analyzer (Agilent Technologies, Palo Alto, CA, USA) and quantified by using Qubit 4.0 Fluorometer (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... and RNA integrity was checked with RNA Kit on Agilent 5300 Fragment Analyzer (Agilent Technologies, Palo Alto, CA, USA). RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina following manufacturer’s instructions (NEB ...
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bioRxiv - Microbiology 2023Quote: ... The TEAD-binding defective HPV8 E6 K136N mutant was made using the QuikChange II site-directed mutagenesis kit (Agilent). Nuclear/cytoplasmic fractionation was performed using the previously published Rapid ...
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bioRxiv - Neuroscience 2023Quote: ... Quantification and quality checked of libraries was done using an Bioanalyzer 2100 instrument and DNA 7500 kit (Agilent Technologies). Libraries were pooled and sequenced in two lanes on a HiSeq 2500 ...
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bioRxiv - Cell Biology 2023Quote: ... The α10-N839Q mutation was generated by QuickChange mutagenesis kit following the instruction (cat# 200517-5, Agilent Technologies, Inc.). All DNA constructs were identified by sanger sequencing service provided by Retrogen ...
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bioRxiv - Systems Biology 2024Quote: ... targeted DNAs were captured and enriched by SureSelect XT HS Target Enrichment Kit ILM Hyb Module according to the manufacturer’s instructions (Agilent). Indexed libraries were quantitated by high sensitivity DNA ScreenTape assay for TapeStation (Agilent) ...
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bioRxiv - Developmental Biology 2024Quote: ... Library fragment size was assessed using Agilent Bioanalyzer 2100 with the High Sensitivity DNA analysis kit (Agilent, 5067-4626) and concentration was determined using Qubit™ dsDNA HS Assay kit (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR products were assessed for purity and concentration on the Bioanalyzer using the High Sensitivity DNA Kit (Agilent Technologies). Samples were multiplexed and sequenced on the Illumina HiSeq 2500 system (Genome Technology Core ...
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bioRxiv - Neuroscience 2023Quote: ... The fragment size of the final libraries was determined using the Bioanalyzer High-Sensitivity DNA Kit (Agilent, CA, USA). Library concentration was determined using the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... RNA integrity was assessed on an Agilent 2100 Bioanalyzer using the RNA 6000 nano kit (Agilent, Santa Clara, CA) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The average library size for the pool was determined using an Agilent DNA D1000 Tape Station kit (Agilent #5067).
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bioRxiv - Biochemistry 2023Quote: ... Gly241Glu and Asp235Ala:Gly241Glu were introduced into the previously published vectors (5) using the QuickChange II Site-directed mutagenesis kit (Agilent) using primers listed in Table S2.
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bioRxiv - Biochemistry 2023Quote: ... The plasmid carrying the WT Cagα protein pHTCagα was amplified using the QuikChange II site-directed mutagenesis kit (Agilent) using 33 bp primers (suppl ...
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bioRxiv - Biophysics 2023Quote: ... The Pro283Ser amino substitution was introduced to this vector via the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent) as previously described (13) ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... was used for the RNA quality control with a microfluidic chip specific for RNA (Agilent RNA 6000 Nano Kit). Then 1 μg of qualified RNA samples in RNase-free ddH2O was used for library preparation ...
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bioRxiv - Biochemistry 2023Quote: The F32A mutants of WT and Δ20-ACA8 were created using a QuikChange site directed mutagenesis kit (Agilent technologies) and the following primers:
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bioRxiv - Cancer Biology 2023Quote: ... their fragment size was analyzed by High Sensitivity NGS Fragment Analysis Kit (DNF-474) on a Fragment AnalyzerTM (Agilent). Libraries were pooled and sequenced with Illumina technology with paired-end reads of 50bp length.
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bioRxiv - Cancer Biology 2023Quote: Site directed mutagenesis was performed on the pEGF-N2-DHRS7 vector according to the manufacturer’s recommendations using the QuikChange II Site-Directed Mutagenesis Kit (Agilent) with primers designed using the Agilent on-line tool (https://www.agilent.com/store/primerDesignProgram.jsp).
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bioRxiv - Cancer Biology 2023Quote: The mitochondrial function of MAFs was measured with a Seahorse XF Cell Mito Stress Test Kit (103015-100, Agilent) using a Seahorse XF96 Extracellular Flux Analyzer ...
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bioRxiv - Neuroscience 2023Quote: ... The fragment size of the final libraries was determined using the Bioanalyzer High-Sensitivity DNA Kit (Agilent, CA, USA). Library concentration was determined using the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... Quantitative PCR was performed using the 1-Step Brilliant II SYBR Green QRT-PCR Master Mix kit (Agilent Technologies) and the TaqPath 1-Step Multiplex Master Mix kit (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... 154 and 175 of Twist1 in the pMPB3 vector were performed using the QuikChange XL site-directed mutagenesis kit accorinding to the manufacturer’s instructions (Stratagene), using the following primers ...
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bioRxiv - Neuroscience 2024Quote: ... or 27 into methionine on histone H3.3A using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies #200521) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: Extracellular flux analysis (Seahorse) was conducted using the Seahorse XF Real-Time ATP rate assay kit (Agilent, 103592-100). The day prior to the assay ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Telomeric FISH using a Cy3-labelled PNA probe was performed according to the manufacturer’s instructions (Telomere PNA FISH Kit/Cy3, Dako), all other methods including the cell cultivation followed the detailed protocols available in Supplementary Data of Altmanová et al ...
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bioRxiv - Microbiology 2024Quote: ... and the quality was assessed using the Agilent RNA 6000 Nano Kit on the Agilent 2100 Bioanalyzer (Agilent Technologies) or Qubit (Thermo Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... gRNA-target-resistant constructs were generated by site directed mutagenesis using QuikChange II Site-directed mutagenesis kit (200521, Agilent) or Q5 Site-directed mutagenesis (E0554 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Amino acid deletions for ΔKPQ were generated by use of the QuikChange Lightning Site-Directed Mutagenesis kit (Agilent, USA). PCR products were cloned into XL-10 Gold Ultracompetent cells ...
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bioRxiv - Cell Biology 2024Quote: ... Libraries were also assessed for size distribution and adapter dimer presence (<0.5%) by the High Sensitivity NGS Fragment 1-6000bp kit on a 96-channel Fragment Analyzer (Agilent). All sequencing libraries were then normalized on the MicroLab STAR (Hamilton) ...
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bioRxiv - Cell Biology 2024Quote: ... and qualitatively assessed with the High Sensitivity NGS Fragment 1-6000bp Kit on a 96-channel Fragment Analyzer (Agilent). Final libraries were normalized ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The final libraries were then quality-checked and quantified for multiplexing using the Bioanalyzer High-Sensitivity DNA kit (Agilent) and Qubit dsDNA HS Assay kit (Thermo Scientific) ...
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Genetic gradual reduction of OGT activity unveils the essential role of O-GlcNAc in the mouse embryobioRxiv - Developmental Biology 2024Quote: ... The integrity of the RNA was determined using the TapeStation 4150 (High Sensitivity RNA kit, Agilent Technologies #5067-5579). Library preparation was performed by EMBL Genomics Core Facility using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760L) ...
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bioRxiv - Immunology 2024Quote: ... PCR fragments were purified with Qiagen MinElute PCR Purification Kit and library concentration and quality were determined by Agilent High Sensitive DNA Kit and TapeStation ...