Labshake search
Citations for Agilent :
5851 - 5900 of 7040 citations for hsa mir 23b Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Each library was quantified by Qubit dsDNA BR or HS Assay kits and assessed by TapeStation using D1000 or HS D1000 DNA tapes (Agilent). was determined using Qubit dsDNA HS Assay Kit ...
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bioRxiv - Biochemistry 2024Quote: All of the mutations were introduced into a plasmid backbone expressing 6xHis tagged pNL4-3-derived IN by QuikChange site-directed mutagenesis kit (Agilent). The plasmids containing full-length WT and mutants were transformed into BL21 (DE3 ...
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bioRxiv - Cancer Biology 2024Quote: ... Integrity was assessed using Agilent RNA 6000 Pico Kit on a 2100 Bioanalyzer instrument (Agilent Technologies, Santa Clara, CA, USA). RNA sequencing libraries were prepared using the Illumina Stranded mRNA prep ligation kit (Illumina ...
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bioRxiv - Cancer Biology 2024Quote: ... The library average fragment size and quantification was performed using Agilent Bioanalyzer High Sensitivity DNA kit (Agilent Cat. 5067-4626) and a final concentration determination was performed using NEBNext® Library Quant Kit for Illumina (New England Biolabs Cat ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries were then quantified using Qubit 1x dsDNA HS Assay Kit for concentration and D1000 ScreenTape (Agilent Technologies, 5067-5582) for length ...
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bioRxiv - Molecular Biology 2024Quote: ... The RNA Integrity Number (RIN) for all samples was 10 as assessed by an RNA Nano Kit (Agilent #5067-1511) on an Agilent Bioanalyzer ...
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bioRxiv - Neuroscience 2024Quote: ... Final library concentration was assessed by Qubit dsDNA HS Assay Kit (Thermo-Fischer Scientific) and fragment size was checked using Tapestation High Sensitivity D1000 (Agilent) to ensure that fragment sizes were distributed normally about 500 bp ...
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bioRxiv - Neuroscience 2024Quote: ... Purified RNA was quantified using a NanoDrop spectrophotometer and RNA quality was further checked with an Agilent 2100 Bioanalyzer using the RNA nano kit (Cat Nº 5067-1511, Agilent). Only samples with RIN>9 were used for library preparation ...
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bioRxiv - Neuroscience 2024Quote: ... Point amino acid substitutions in genes encoding GluN subunits were performed using the QuikChange site-directed mutagenesis kit (Agilent Technologies) and verified by DNA sequencing.
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bioRxiv - Neuroscience 2023Quote: ... Patient M03’s DNA was captured with the SureSelect Human All Exon V6 Kit (Agilent Technologies, Santa Clara, CA, USA) and sequenced on a NextSeq500 (Illumina ...
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bioRxiv - Molecular Biology 2024Quote: Primary mouse AT2s were isolated and plated as described above with measurement of mitochondrial respiration performed using the Seahorse XF Cell Mito Stress Test Kit (Agilent) or the Seahorse XF Palmitate Oxidation Stress Test Kit (Agilent ...
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bioRxiv - Molecular Biology 2024Quote: ... at a ratio of 0.8:1 beads:cDNA (20 μl beads:25 μl cDNA for Smart-seq2 and 8 μl beads:10 μl cDNA for Smart-seq3) and inspected on a Bioanalyzer 2100 (High Sensitivity DNA kit, Agilent).
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bioRxiv - Cell Biology 2024Quote: ... Mitochondrial energetics was measured using a Seahorse XFe96 extracellular flux analyzer and the XF Cell Mito Stress kit (Seahorse Bioscience) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... pcDNA3.1zeo+/ nFlag-cmScarlet-hRobo1 was generated by inserting the Flag tag sequence at the N-terminus after the signal peptide sequence of Robo1 with QuikChange II Site-Directed Mutagenesis Kit (Agilent Technologies ...
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bioRxiv - Immunology 2024Quote: ... residues D141 of Regnase-1 and D252 of Regnase-3 were mutated to asparigines using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent) following manufacturer’s protocol ...
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bioRxiv - Genetics 2024Quote: Membranes were hybridized with “probe 4” for the HIS4::LEU2 hotspot 100 which was 32P-dCTP labelled using the Prime-It II Random Primer Labeling Kit (Stratagene). Following hybridization ...
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bioRxiv - Developmental Biology 2024Quote: The quality of the cDNA and resulting sequencing libraries were checked by bioanalyzer (High Sensitivity DNA Kit, Agilent, 5067-4626). The libraries were sequenced using Illumina NovaSeq PE150 ...
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bioRxiv - Genetics 2024Quote: ... The SCN5A mutations E171Q (Ensembl transcript ENST00000333535 c.511G>C) and R222Q (c.665G>A) were introduced with a QuikChange Multi Lightning kit (Agilent) with primers 5’ttcaccgccatttacacctttCagtctctggtca (E171Q ...
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bioRxiv - Immunology 2024Quote: ... and RNA integrity was assessed with Agilent RNA 6000 nano kit (Aligent, Cat. No. 5067-1511) using an Agilent 2100 bioanalyzer (Agilent). The average A260/280 ratio was 1.9 (range 1.8-2.1) ...
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bioRxiv - Microbiology 2024Quote: ... The plasmids expressing the BA.5 spike with the A570S mutation or the A570S/K147E double mutation were obtained by site-directed mutagenesis using a QuikChange II Site-Directed Mutagenesis Kit (Agilent). The dual split protein (DSP ...
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bioRxiv - Immunology 2024Quote: ... Mitochondrial stress test assay for pDC on the XF-HS Mini was optimized in-house within the ranges provided by the manufacturer and performed using the mitochondrial stress test kit (Agilent), with the addition of 2-DG (Millipore Sigma ...
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bioRxiv - Microbiology 2024Quote: ... Library size was validated by using Fragment Analyzer 48-Capillary Array with the HS NGS Fragment Kit (1-6000bp) (Agilent). Libraries were sequenced with a Nextseq 2×150 pb paired-end reagent kit (NextSeq 500/550 High Output Kit v2.5 ...
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bioRxiv - Microbiology 2023Quote: ... and quantity of both total and PolyA+ RNA samples were assessed using the Agilent 2100 Bioanalyzer with the RNA 6000 Nano LabChip Kit (Agilent), the NanoDrop 1000 Spectrophotometer (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2024Quote: ... RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). ii ...
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bioRxiv - Cell Biology 2024Quote: Whole exome sequencing on genomic DNA of the patient was performed using the SureSelect Human All Exon V6 kit (Agilent) on an Illumina HiSeq 4000 sequencer ...
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bioRxiv - Neuroscience 2023Quote: Point mutations were introduced into Stx1A or Stx2 using a TA cloning vector with the QuickChange Site-Directed Mutagenesis Kit (Stratagene). Chimeric constructs of both syntaxin isoforms were obtained using Gibson Assembly (NEB) ...
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bioRxiv - Developmental Biology 2023Quote: ... Libraries were analysed on the 2100 Bioanalyzer Agilent system using the Agilent High Sensitivity DNA kit (Agilent Technologies, 5067-4626). DNA concentrations were measured by Qubit Quant IT kit (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... the codon for C34 in pCEP4-MFAP4 was modified from TGC to TCG using the QuickChange Site Directed Mutagenesis kit as instructed by the manufacturer (Agilent). The entire insert in pCEP4-MFAP4 and the sequence coding for the C34S mutation in pCEP4- MFAP4C34S was validated by Sanger DNA sequencing ...
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bioRxiv - Genetics 2023Quote: ... The quality and concentration of the library were assessed using the Femto Pulse Genomic DNA 165 kb Kit (Agilent Technologies) and Qubit dsDNA HS Assay (ThermoFisher) ...
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bioRxiv - Genomics 2023Quote: ... the concentration and integrity of the RNA were assessed using the ND-1000 UV-visible light spectrophotometer from Nanodrop Technologies and the Bioanalyzer 2100 with the RNA 6000 Nano Lab Chip kit by Agilent.
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bioRxiv - Cell Biology 2023Quote: ... The mutations corresponding to the MARylation sites of RACK1 (D144N/E145Q/D203N) were introduced into the pCMV3-RACK1 plasmid using the QuikChange Site-Directed Mutagenesis kit (Agilent).
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bioRxiv - Immunology 2023Quote: ... Specific nucleotide substitutions or coding-sequence truncations were introduced as required using the QuikChange XL Site-Directed Mutagenesis Kit (Agilent). All plasmid inserts and modifications were authenticated by Sanger sequencing.
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bioRxiv - Genomics 2023Quote: ... The integrity of the RNA sample was verified using the Agilent RNA 6000 kit and Agilent Bioanalyzer 2100 (Agilent Technologies). Samples with an RNA Integrity Number (RIN ...
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bioRxiv - Genomics 2023Quote: ... Mean length and library concentration was determined by running libraries on a 2100 bioanalyzer using a DNA 1000 Kit (Agilent). RNA-seq libraries were sequenced by the Norwegian Sequencing Center (Oslo ...
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bioRxiv - Immunology 2024Quote: ... The resulting libraries tagged with unique dual indices were checked for size and quality using the Agilent High Sensitivity DNA Kit (Agilent). Library concentrations were measured using the KAPA SYBR Fast qPCR kit and loaded for sequencing on the Illumina NovaSeq 6000 instrument (paired-end 2X150 ...
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bioRxiv - Cancer Biology 2023Quote: The metabolic profile of 1205Lu melanoma cells was assessed using the Seahorse XF Cell Energy Phenotype Test Kit (Cat no.103325-100, Agilent). Both parental and NKX2.2 KO 1205Lu cells ...
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bioRxiv - Neuroscience 2023Quote: ... RNA integrity of brain organoids and mouse tissue was evaluated by analysis on the 2100 Bioanalyzer RNA 6000 Pico Chip kit (Agilent) using the RNA Integrity Number (RIN) ...
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bioRxiv - Cancer Biology 2023Quote: ... Staining visualisation was performed by incubating with a cocktail of anti-mouse and anti-rabbit secondary antibodies conjugated to horseradish peroxidase (Envision Kit, Agilent) for 60 minutes at room temperature ...
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The effects of caloric restriction on adipose tissue and metabolic health are sex- and age-dependentbioRxiv - Physiology 2023Quote: ... and RNA Integrity Number (RIN) was assessed using Agilent RNA 6000 Pico Kit and an Agilent 2100 bioanalyzer (Agilent, USA). Library preparation and sequencing was performed by BGI (Shenzhen ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA was extracted using the same kit according to manufacturer’s instructions and quality was assessed using the Agilent 2200 TapeStation System (Agilent, G2964AA) with RNA ScreenTape (Agilent ...
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bioRxiv - Pathology 2023Quote: ... RNA Integrity Number (RIN) and DV200 were determined by BioAnalyzer electrophoresis using an RNA 6000 Pico Kit (#5067-1513, Agilent). The DV200 metric refers to the percentage of total profiled RNA fragments greater than 200bp in length ...
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bioRxiv - Systems Biology 2022Quote: ... Adherent NSCs were infected with retro-Cre-Tomato retrovirus (5μl of virus was used to infect 3x105 cells) or nucleofected with expression constructs according to instructions of the Amaxa nucleofection kit (Agilent) [28] ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Amplified and purified sequencing libraries were analyzed on an Agilent 2100 Bioanalyzer (High sensitivity DNA analysis kit, Agilent, 5067-4626), pooled in equimolar amounts (2.47 ng/μL) ...
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bioRxiv - Molecular Biology 2022Quote: ... Point mutations in the repM gene were generated using specific primers and a QuikChange Site-Directed Mutagenesis kit according to the protocol supplied by the manufacturer (Stratagene). All oligonucleotide primers used in this study are listed in Supplementary Table S2.
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bioRxiv - Cancer Biology 2023Quote: ... The stranded mRNA libraries were analyzed on a 2100 Bioanalyzer instrument following the manufacturer’s protocol “Agilent DNA 7500 kit” (G2938-90024, Agilent Technologies), diluted to 10nM and pooled equimolar into multiplex sequencing pools for paired end sequencing on the NovaSeq 6000 Illumina sequencing instrument ...
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bioRxiv - Immunology 2023Quote: ... All mutations in this study were introduced using the QuikChange II XL site-directed mutagenesis kit (Agilent Technologies, Cat #200521), KLD Enzyme Mix ...
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bioRxiv - Immunology 2022Quote: ... SARS-COV-2-Strunc variants were generated in house by site-directed mutagenesis (QuikChange Multi Site-Directed Mutagenesis Kit, Agilent) starting from synthetic DNA (Genscript) ...
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bioRxiv - Molecular Biology 2023Quote: ... and C66A triple mutation), KR5>A (contains K286A, R287A, K288A, K300A, and R301A mutations) were done using QuikChange Mutagenesis Kit (Agilent). Deletion mutants (1-280 ...
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bioRxiv - Microbiology 2023Quote: ... The resulting pHD1344tub(PAC)-mt-LAF3-Cterm3V5 plasmid was then used as a template for site-directed mutagenesis of D211 (QuikChange II kit; Agilent) using forward and reverse primers listed in Supplemental Table 1 ...
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bioRxiv - Molecular Biology 2023Quote: ... FLAG-tagged human SLFN14 D249A was generated by site directed mutagenesis with the QuickChange Lightning Site-Directed Mutagenesis Kit (Agilent) using reverse primer SS1 (5’-atccaccccaatgaggacatatcc-3’ ...