Labshake search
Citations for Agilent :
5851 - 5900 of 5930 citations for Rat N acylethanolamine hydrolyzing acid amidase NAAA ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2023Quote: ... RNA concentration was determined using an RNA 6000 Pico Kit run on a Bioanalyzer 2100 running Agilent 2100 Expert Software (B.02.11; Agilent Technologies, Santa Clara, CA, USA). RNA was converted to cDNA using iScript cDNA Supermix (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: ... The hybridization was performed according to the manufacturer instructions on 4×180K mouse microarrays (SurePrint G3 Mouse CGH Microarray Kit, 4x180K, AGILENT Technologies, reference genome: mm9). Microarrays were scanned with an Agilent High-Resolution C Scanner using a resolution of 3 µm and the autofocus option ...
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bioRxiv - Physiology 2023Quote: ... Standard curves for each gene were generated by cloning qPCR products into the pSCA vector with the Strataclone PCR cloning kit (Agilent, Santa Clara, CA, USA), isolating plasmid DNA using the GeneJET Plasmid Miniprep Kit (Thermo Scientific ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Aliquots of ∼0.1 g were used for total RNA (RNAt) extractions with the Agilent Plant RNA isolation Mini kit (Agilent Technologies, Santa Clara CA, USA). Three aliquots of RNAt per sample were separated and their concentrations adjusted to 50 ng μL−1.
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bioRxiv - Neuroscience 2023Quote: ... Quality control of total RNA quality and quantity was done on the Agilent 2100 Bio analyzer (Agilent RNA 6000 Nano Kit, Cat# 5067-1511) QC ...
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bioRxiv - Cancer Biology 2024Quote: ... Extracts from tumors and media were analyzed using the Absolute IDQ p180 kit (Biocrates Life Sciences AG, Innsbruck, Austria) with an Agilent 1290 Infinity UHPLC system (Agilent, Santa Clara, CA, USA) coupled with an MS/MS Sciex Triple Quad 6500 (AB Sciex LLC ...
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bioRxiv - Plant Biology 2024Quote: ... concentration and integrity were evaluated on 0.8% agarose gels (v/w) and with the Bioanalyzer RNA Nano 6000 Assay Kit (Agilent Technologies, Santa Clara, CA, USA). RNA sequencing was performed using Illumina NovaSeq 6000 platform ...
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bioRxiv - Immunology 2023Quote: ... The oxygen consumption rate (OCR) and the extracellular acidification rate (ECAR) were measured with an XF Cell Mito Stress Kit (Agilent, Santa Clara, CA, USA) as per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Basal ATP production rates were calculated using the Seahorse XF Real-Time ATP Rate Assay Kit Report Generator (Wave Desktop Software, Agilent Technologies, Cedar Creek, TX). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: ... The quality and concentration of both cDNA and libraries were assessed using an Agilent BioAnalyzer with High Sensitivity kit (#5067-4626, Agilent, Santa Clara, CA USA) and Qubit Fluorometer with dsDNA HS assay kit (#Q33230 ...
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bioRxiv - Cell Biology 2023Quote: Completed sequencing libraries were quantified using the Qubit Fluorometer and the Qubit dsDNA HS Assay Kit and fragment length distributions assessed using the Agilent 4200 TapeStation and the High Sensitivity D1000 ScreenTape Assay (Agilent; 5067-5584, 5067-5585).
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bioRxiv - Plant Biology 2023Quote: ... isolated and DNAse treated RNA extracted from 5-week-old plants was assayed for RNA integrity with the Agilent 2100 Bioanalyzer using the RNA Nano 6000 kit (Agilent Technologies, Santa Clara, USA). Single-end RNA-seq was performed on a HiSeq 2500 High Output V4 platform (Illumina ...
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bioRxiv - Microbiology 2024Quote: ... Plasmids harboring mutated sRNA alleles under the control of the PLlac promoter were constructed using the QuikChange II XL site-directed mutagenesis kit (Agilent Technologies, Santa Clara, CA) with oligonucleotides that contain mismatched bases at the desired locations (Table S2 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Point mutation of D5R-STOP from D5-TANGO was made by polymerase chain reaction (PCR) using the QuikChange II Site–Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2024Quote: ... Point mutations were induced using either the QuikChange II XL Site-Directed Mutagenesis or Multi Site-Directed Mutagenesis Kit (Agilent Technologies, Cedar Creek, TX). Subsequently ...
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bioRxiv - Cancer Biology 2024Quote: ... the cDNA was purified by SPRIselect Reagent (Cat# B23318) and quantified using Picogreen™ dsDNA assay kit (Cat# P7589) and quality assessed using High Sensitivity D5000 ScreenTape (Agilent, Cat# 5067-5592) with High Sensitivity D5000 Reagents (Agilent ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Aliquots of ∼0.1 g were used for total RNA (RNAt) extractions with the Agilent Plant RNA isolation Mini kit (Agilent Technologies, Santa Clara CA, USA). Three aliquots of total RNA (RNAt ...
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bioRxiv - Neuroscience 2024Quote: ... The library quality was evaluated by measuring the library size in base pairs using the Agilent High Sensitivity DNA Kit and Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA). During the library construction ...
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bioRxiv - Cell Biology 2024Quote: ... Sample amplification and labeling were carried out using the Low Input Quick Amp labeling Kit (Catalog number 5190-2305, Agilent Technologies, Santa Clara, CA). Briefly ...
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bioRxiv - Immunology 2024Quote: RNA from splenocytes isolated from UVB exposed WT and Trim21-/-mice were analyzed for RNA integrity on the 2100 Bioanalyzer using the Agilent RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA) and quantified using the Qubit RNA HS Assay Kit (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: Deletion mutants of two TCF4 binding sites (TBE1 and TBE2) on the STUB1 promoter were generated as previously performed [25] using the Quickchange XL Site directed mutagenesis kit (Agilent technologies, Santa Clara, CA, USA) as per the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... the material was loaded onto denaturing PolyAcrylamide Gel Electrophoresis (dPAGE) at 12% or analyzed using the Agilent RNA 6000 Pico LabChip kit on an Agilent 2100 Bioanalyzer (Agilent Technology, Palo Alto, CA, USA). Of course ...
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bioRxiv - Molecular Biology 2022Quote: ... Ser181Ala;Thr100Ala double mutant and Ser181Ala;Thr100Ala;Thr692Ala triple mutant were prepared using the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA, US) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... fragment distribution of the gDNA library was measured using the DNA 1000 Assay Kit with the Agilent Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA, USA). DNA libraries were sequenced with 150 base pair (bp ...
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bioRxiv - Molecular Biology 2020Quote: ... One microliter of the purified RNA solution was used for electrophoresis using a Bioanalyzer 2100 with Agilent RNA nano or pico kit (Agilent Technologies, Santa Clara, CA, USA) to check for the quality.
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bioRxiv - Molecular Biology 2021Quote: ... the steps were as follows: a) quality control using the Agilent 2100 Bio analyzer (Agilent RNA 6000 Nano Kit, Agilent Technologies, Santa Clara, CA, USA), b ...
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bioRxiv - Molecular Biology 2020Quote: ... fluorescent chemistry and 1 ng was used to obtain RNA Integrity Number (RIN) using the Bioanalyzer RNA 6000 Pico kit (cat # 5067-1513, Agilent Technologies Inc., Santa Clara, USA). Lowest RIN was 9.3 ...
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bioRxiv - Genetics 2019Quote: Site-directed mutagenesis for obtaining CDK2-Y15F and CDK2-Y15S cDNA was accomplished using the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent, La Jolla, CA; Cat# 210518) using primers sets mentioned in Table S3 ...
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bioRxiv - Cancer Biology 2019Quote: ... RNA was quantified using Nanodrop spectrophotometer ND-1000 and the integrity and purity were assessed by the Agilent 2100 Bioanalyzer and RNA 6000 Nano Labchip Kit (Agilent Biotechnologies, Palo Alto, CA, USA).
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bioRxiv - Microbiology 2019Quote: ... Quantity and quality of total RNA and depleted RNA samples were assessed using the RNA 6000 Nano Lab Chip Kit on a Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA). Library preparation and sequencing were performed by SciLife (Stockholm ...
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bioRxiv - Plant Biology 2019Quote: ... The quality and the quantity of cDNA was checked using the Agilent 2100 Bioanalyzer with the High Sensitivity DNA kit (Agilent Technologies, Santa Clara, CA, USA). cDNA was sheared using the COVARIS S2 system (Covaris Inc. ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and the quality and quantity of that pool was assessed via electrophoresis (High-Sensitivity DNA Kit and Agilent Bioanalyzer; Agilent Technologies, Santa Clara, CA, USA), real-time quantitative polymerase chain reaction (qPCR ...
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bioRxiv - Immunology 2021Quote: ... The library was hybridized to biotinylated cRNA oligonucleotide baits from the SureSelect Human All Exon kit V6+somatic probe sets (Agilent Technologies Inc., Santa Clara, CA), and amplified for 12 cycles ...
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bioRxiv - Immunology 2021Quote: ... RNA quantification and quality assessments were performed by ultraviolet–visible spectrophotometry (Nanodrop Technologies, Wilmington, DE) and the RNA 6000 Nano Chip Kit with the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). RNA quality of all samples reached a RNA integrity number (Agilent Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... fluorescent chemistry and 1 ng was used to obtain RNA Integrity Number (RIN) using the Bioanalyzer RNA 6000 Pico kit (cat # 5067-1513, Agilent Technologies Inc., Santa Clara, USA). Lowest RIN was 9.1 ...
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bioRxiv - Genetics 2022Quote: ... The purified RNA quality was evaluated through capillary electrophoresis in the Bioanalyzer 2100 with an RNA 6000 Nano LabChip Kit (Agilent Technologies, Santa Clara, CA, USA) and quantified using a Nanodrop spectrophotometer ...
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bioRxiv - Genomics 2020Quote: ... The quality and yield of the prepared libraries were assessed using a high sensitivity Small DNA Fragment Analysis Kit (Agilent Technologies, Santa Clara, CA, USA) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Samples were labeled for microarray analysis using the One-Color Microarray-Based Gene Expression Analysis Low Input Quick Amp WT Labeling kit (Agilent Technologies, Santa Clara, CA, USA), according to the manufacturer’s instructions.
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bioRxiv - Genetics 2022Quote: ... The length of the eluted DNA fragments was measured on a Femto Pulse using the Agilent Genomic DNA 165 kb kit (Agilent Technologies, Santa Clara, CA, USA) according to the manufacturer’s recommendations.
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bioRxiv - Cancer Biology 2022Quote: ... for 1 hour at room temperature followed by treatment with HRP-conjugated anti-mouse secondary antibody (DAKO Envision Plus HRP kit, Dako Denmark A/S, Glostrup, Denmark) for 30 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... RNA yield was quantified with a NanoDrop Spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA) and RNA integrity was assessed with Bioanalyzer 2100 RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA). All samples had an RNA Integrity Number (RIN ...
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bioRxiv - Microbiology 2021Quote: ... Samples were eluted in RNAse-free water and purified RNA was analyzed using Qubit RNA HS Assay Kit (Thermo) and Agilent TapeStation 4200 (Agilent Technologies, Santa Clara, CA, USA) using the RNA High Sensitivity assay ...
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bioRxiv - Microbiology 2019Quote: ... cDNA of each sample was synthesized from 10 μg of total RNA using the FairPlay III Microarray Labeling Kit (Agilent Technologies, Santa Clara, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... A mouse monoclonal anti-p63 antibody (1:500, MS-1081-P, Neomarkers, Fremont, CA, USA) and followed by a secondary kit (Mouse Envision, DAKO-Agilent, Santa Clara CA, USA), stained with chromogen (3,3′-Diaminobenzidine [DAB] ...
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bioRxiv - Cell Biology 2021Quote: ... according to the manufacturer protocol and complementary DNAs were generated using an AccuScript High Fidelity 1st Strand cDNA Synthesis kit (Agilent Technologies, Santa Clara, VA, USA). A quantitative PCR was performed using a QuantStudio™ 3 Real-Time PCR system (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2020Quote: ... RNA integrity was defined by capillary electrophoresis with an RNA 6000 Nano Lab-on-a-Chip kit and Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA). Only if the integrity number values of RNA were more than 6 ...
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bioRxiv - Microbiology 2019Quote: ... the material was loaded onto denaturing polyacrylamide gel electrophoresis (dPAGE) at 12% or analysed using the Agilent RNA 6000 Pico LabChip kit on an Agilent 2100 Bioanalyzer (Agilent Technology, Palo Alto, CA, USA). Controls were carried out under the same conditions ...
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bioRxiv - Microbiology 2020Quote: Single point SCoV2-NTD-Fc N-linked glycosylation site mutants (N61, 74, 122, 149, 165, 234, 282Q) were generated using a QuikChange site-directed mutagenesis kit (Agilent Technologies, Santa Clara, CA, USA) to change the respective asparagine (N ...
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bioRxiv - Microbiology 2020Quote: ... The synthesis and fluorescence labeling of cDNA followed a strand-specific method using the FairPlay III Microarray Labeling Kit (Agilent Technologies, Santa Clara, CA, USA) and actinomycin D (Calbiochem ...
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bioRxiv - Microbiology 2020Quote: ... and D67N/K70R/T215F/K219Q viruses were generated by site-directed mutagenesis on an xxLAI HIV-1 backbone using the QuikChange XL Site-Directed Mutagenesis kit (Agilent Technologies Inc, Santa Clara, CA), according to the manufacturer’s protocols.