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Citations for Agilent :
5401 - 5450 of 5930 citations for Rat N acylethanolamine hydrolyzing acid amidase NAAA ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... pcDNA3.1-HA-SorCS1cβ Y1132A and GFP-tagged Rab7 T22N were generated by in vitro mutagenesis using the QuikChange II Site-Directed Mutagenesis Kit (Agilent, Cat #200522) from pcDNA4-SorCS1cβ-myc (kindly provided by Alan D ...
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bioRxiv - Biochemistry 2019Quote: ... and triple mutant D65K/E66K/E67K (DEE/KKK) – were generated by site-directed mutagenesis (Quick Change Lightning Site directed Mutagenesis Agilent Kit 210518) using the pLew100-TbBILBO1 plasmid and transfection of the Tb427 29.13 cell line [10] ...
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bioRxiv - Cell Biology 2019Quote: The oxygen consumption rate (OCR) and the extracellular acidification rate (ECAR) were measured using the Mito Stress Test kit (Agilent, #103010-100), following the manufactureŕs instructions ...
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bioRxiv - Genetics 2019Quote: ... The integrity of RNA was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA).
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bioRxiv - Genomics 2019Quote: ... we only analyzed regions overlapping between RNA-seq (with more than 10x read coverage in annotated exons) and the WES enrichment kit (Agilent SureSelect 71Mb). Again ...
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bioRxiv - Biochemistry 2020Quote: The oxygen consumption rate (OCR) of LPO-exposed HLCs was measured using the Agilent Seahorse XF Cell Mito Stress Test Kit (Agilent, 103015-100) on a Seahorse XF Analyser (Agilent ...
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bioRxiv - Biochemistry 2020Quote: ... The mutations to generate Asp276AlaSd and Tyr435HisSd were introduced by the QuikChange II Lightning Site-Directed Mutagenesis kit (Agilent, Santa Clara, CA) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... Cy3-labeled cRNA was generated from 200 ng input total RNA using Agilent’s Low Input Quick Amp Labeling Kit (Agilent Technologies, Tokyo, Japan). cDNA was generated with the primer containing a T7 polymerase promoter ...
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bioRxiv - Genomics 2021Quote: ... Each sample was prepared according to Agilent library preparation protocols (Human All Exon 50 Mb kit; Agilent Technologies, Santa Clara, CA, USA). Libraries were subjected to paired-end sequencing on an Illumina Hiseq 2000 and 2500 instrument (Illumina ...
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bioRxiv - Cancer Biology 2020Quote: ... and cRNA was hybridized with ArrayXS Human (Oaklabs, Germany) at 65 °C for 17 hours using the Agilent Gene Expression Hybridization Kit (Agilent Technologies, USA), washed once with Agilent Gene Expression Wash Buffer 1 for one minute at room temperature ...
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bioRxiv - Immunology 2021Quote: ... RNA integrity and quantitation were assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). A total amount of 1 μg RNA per sample was used as input material for the RNA sample preparations ...
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bioRxiv - Neuroscience 2020Quote: CLC-2 mutants were generated by site-directed mutagenesis using a QuickChange XL Site-Directed Mutagenesis Kit (Agilent Technologies, Cedar Creek, TX). The following table lists primers used to generate expression vectors in this study.
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Differential impact of a dyskeratosis congenita mutation in TPP1 on mouse hematopoiesis and germlinebioRxiv - Cell Biology 2021Quote: ... One set of single and mixed samples was stained with a FITC PNA probe and the other set was left unstained to correct for auto-fluorescence (Dako telomere kit). DNA was denatured for 10 min at 82°C and allowed to hybridize overnight ...
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bioRxiv - Bioengineering 2020Quote: ... RNA quality after purification was assessed using an Agilent 2100 Bioanalyzer and RNA 6000 Pico Kit for low sample quantities (Agilent Technologies, USA) providing data on the integrity of RNA (RNA Integrity Number ...
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bioRxiv - Bioengineering 2020Quote: ... LEU4 and Ll_ilvD were generated by error-prone PCR using the GeneMorph II Random Mutagenesis Kit (Agilent Technologies, Santa Clara, CA, USA). The CEN plasmids harboring wild-type ILV6 (pYZ127) ...
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Persistent DNA damage rewires lipid metabolism and promotes histone hyperacetylation via MYS-1/Tip60bioRxiv - Cell Biology 2021Quote: ... RNA integrity and quantitation were assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA).
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bioRxiv - Neuroscience 2020Quote: ... The number of PCR amplification cycles was adjusted for each sample group based on the number of cycles needed to detect RNA in the lowest input sample as determined by Bioanalyzer High Sensitivity DNA Kit (Agilent #5067-4627). Negative controls for each group were run in parallel to ensure that additional cycles did not result in unspecific background product ...
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bioRxiv - Cancer Biology 2021Quote: Mitochondrial respiration and glycolysis were assessed using Seahorse extracellular flux analyzer (XFe96) and Seahorse XF Cell Mito Stress Test Kit (Agilent Technologies, USA). Cells were seeded in 96-well plates (2 × 104 cells/well) ...
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bioRxiv - Cancer Biology 2020Quote: ... Quality control of RNA integrity and total RNA concentration was then measured using the Bioanalyzer Eukaryote Total RNA Pico kit (Agilent #5067-1513). Only samples with RIN values of 8.0 or greater were submitted for sequencing ...
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bioRxiv - Cell Biology 2021Quote: Point mutations were introduced into the wild-type human Myo1e in the pEGFP-C1 vector using QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent Technologies #210519) [7 ...
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bioRxiv - Neuroscience 2020Quote: DNA isolation of FACS-enriched tdTomato cells was performed using QIAamp DNA Micro Kit (Quiagen, Germany) according to manufacturer’s instruction and checked for integrity by capillary gel electrophoresis (Bioanalyzer, Agilent Technologies, Inc., USA).
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bioRxiv - Neuroscience 2020Quote: ... the staining was revealed by 30 min incubation in a HRP-anti rabbit polymer system (DAKO REAL Envision™ Kit, #K400311-2, Agilent, France) followed by a DAB revelation of few seconds (DAKO DAB Kit ...
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bioRxiv - Biochemistry 2021Quote: Plasmids encoding AlgE7 variants (Table 2) were constructed based on wild type AlgE7 (plasmid pBG27) (12) using the QuikChange™ Site-directed Mutagenesis Kit (Stratagene/Agilent) or Q5 site directed mutagenesis (New England Biolabs) ...
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bioRxiv - Plant Biology 2021Quote: ... The extracted RNA was assayed for RNA integrity on 2100 Bioanalyzer using RNA 6000 Pico Kit (Agilent Technologies, Santa Clara, CA, USA). RNA samples were depleted for ribosomal RNAs (rRNAs ...
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bioRxiv - Cell Biology 2021Quote: ... RNA integrity and quantitation were assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). A total amount of 1 μg RNA per sample was used as input material for the RNA sample preparations ...
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bioRxiv - Cell Biology 2021Quote: ... Libraries were then analyzed by qPCR generating CT values using NEBNEXT Library Quant Kit (#E7630) and by tape station with D1000 screen tape from Agilent (#5067-5582) to calculate fragment base pair size ...
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bioRxiv - Cell Biology 2021Quote: ... The substitution S59D and S59A were introduced into the pLVX-IRES-FLAG-tagged HERPUD1 vector using the QuikChange II XL direct-mutagenesis kit (Stratagene, cat#200522) and the mutagenesis service of GenScript (Hong Kong ...
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bioRxiv - Plant Biology 2021Quote: ... Grown colonies were screened with colony PCR using CP-F and UnivR primers and KAPA2G Robust HotStart Kit (Agilent, Supplemental Method 2). Sanger sequencing of the selected clone confirmed correct sequence of the PVY-coding part and correct in-frame insertion of GFP coding sequence ...
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bioRxiv - Plant Biology 2021Quote: ... needles and green stem) of 4 individual clonal Pinus radiata trees using the Plant RNA Isolation Mini Kit (Agilent, Santa Clara, CA). 50 mg of plant material from each tissue type were used for the RNA extractions ...
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bioRxiv - Microbiology 2020Quote: ... Mutations were made in the pET-51b containing the wild type DesC using QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent, Santa Clara) according to the manufacture’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... total RNA concentration and integrity were quantified using a RNA 6000 Nano Kit 2100 on a Bioanalyzer Instrument (Agilent Technologies, CA, USA).
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bioRxiv - Developmental Biology 2021Quote: ... Deletion and mutation variants were generated using standard cloning techniques and the QuickChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies, 200521).
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bioRxiv - Immunology 2020Quote: ... RNA integrity was analyzed on a subset of samples using 2100 Bioanalyzer and the RNA 6000 Nano kit (Agilent, Santa Clara, CA). Reverse transcription and cDNA synthesis was performed on 200ng of RNA using Maxima First Strand cDNA synthesis kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Sections labelled for F4 were incubated with secondary antibody from kit polymer-HRP anti-rabbit (Dako En Vision+ System-HRP, Dako, Glostrup, Denmark) while sections labelled for CD3 were incubated with anti-mouse biotinylated secondary antibody (catalogue number BA-2000-1.5 Vector Laboratories ...
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Induction of Dopaminergic Neurons for Neuronal Subtype-Specific Modeling of Psychiatric Disease RiskbioRxiv - Neuroscience 2021Quote: ... The yield and quality of the resulting RNA samples were assessed with the Agilent Bioanalyzer using an Agilent RNA 6000 Pico kit (Agilent, #5067-1513). Ribosomal rRNA-depleted RNAseq libraries were prepared using the SMARTer Stranded RNA-Seq kit (Clontech ...
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bioRxiv - Cancer Biology 2020Quote: ... RNA integrity and quantitation were assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). For each sample ...
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bioRxiv - Cell Biology 2021Quote: ... pIRESneo2-EGFP-TOPBP1-ΔBRCT6 was generated by site directed mutagenesis of pIRESneo2-EGFP-TOPBP1-WT using QuikChange II XL Site-Directed Mutagenesis kit (Agilent Technologies, 200522).
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bioRxiv - Cell Biology 2021Quote: ... Entry clones for other mutant dynamin 2 were prepared by introducing corresponding mutations into the Entry clone of wild type human dynamin 2 using QuikChange Lightning Site-directed Mutagenesis kit (Agilent Technologies, 210518) following manufacturer’s instruction ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA libraries were assessed for quality and quantification according to the Chromium Single Cell 3’ Reagent kit v3 user guide using the High Sensitivity D5000 ScreenTape (Agilent 5067-5592) and 2200 TapeStation Controller software (Agilent ...
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bioRxiv - Cancer Biology 2021Quote: ... was labeled with Cy3 and the ATAC-libraries were labeled Cy5 using Genomic DNA ULS labeling kit (Agilent Technologies, catalog # 5190-0420). After estimating the labeling efficiencies independently by nanodrop ...
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bioRxiv - Cell Biology 2022Quote: ... RT-qPCR was performed using SYBR® Green Premix Pro Taq HS qPCR Kit (Accurate biology, China) on an AriaMx Real-time quantitative PCR machine (Agilent, USA). The PCR reaction conditions were 95°C for 30 s ...
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The Batten disease protein CLN3 is important for stress granules dynamics and translational activitybioRxiv - Molecular Biology 2022Quote: The measurement of rates of oxidative phosphorylation and glycolysis in wild type HeLa cells and CLN3 KO HeLa cells was carried out using the Agilent Seahorse XF Cell Energy Phenotype Kit (Agilent #103325-100) using the Agilent Seahorse XFe 96 Analyzer ...
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bioRxiv - Genetics 2022Quote: ... HIRA-HA mSIM and K809G mutants were obtained by site-directed mutagenesis using QuickChange Lightning Site-directed Mutagenesis kit (Agilent Technologies, #210518). SIM motifs are characterized by a group of hydrophobic amino acids ((V/I/L)x(V/I/L)(V/I/L ...
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bioRxiv - Molecular Biology 2022Quote: ... The quality and acquired amount of the amplicons was assessed using Agilent High Sensitivity DNA Chip kit on Agilent 2100 BioAnalyzer (Agilent Technologies, USA) and Qubit dsDNA HS Assay Kit on Qubit 2.0 Fluorimeter (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... All other constructs were made by PCR amplification followed by standard restriction enzyme cloning or by site-directed mutagenesis kit (QuickChange II, Agilent Technologies, 200523). All oligonucleotides were obtained from Sigma-Aldrich ...
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bioRxiv - Bioengineering 2022Quote: The quality of each final library was assessed using the Agilent® dsDNA High Sensitivity Kit (Agilent CA, USA, CAT#5067-4626). A typical Ampliseq Trancriptome library is shown in Supplemental Figure 1 ...
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bioRxiv - Developmental Biology 2022Quote: ... and the quality of RNA was assessed using an Agilent fragment analyzer and the RNA Analysis Kit (15 nt; Agilent, DNF-471). cDNA libraries were generated using the Kapa mRNA HyperPrep kit with mRNA capture (KapaBiosystems ...
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bioRxiv - Cell Biology 2022Quote: ... and antisense primer (5’-GGA GAA AGG CAC CTG CCA GGC CTC AAC-3’) by site-directed mutation according to manufacturer’s protocol (QuikChange II XL Site-Directed Mutagenenesis Kit, 200521; Stratagene, LA Jolla, CA). The primers were designed to delete (in frame ...
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bioRxiv - Cancer Biology 2022Quote: ... A BsrG1 site within the TOLLIP cDNA was eliminated by mutation using the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent, Catalog #210518) as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... The RIOK1 Ser22 to Ala mutant and Ser21/22 to Ala double mutant were generated using QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent, Catalog #210518). Primers were designed using the QuikChange Primer Design website ...