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Citations for Agilent :
5701 - 5750 of 8383 citations for Human Adenosylhomocysteinase Like 1 AHCYL1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Zoology 2023Quote: ... 1×Brilliant III Ultra-Fast SYBR Green qPCR Master Mix (Agilent Technologies 600882) was used with intron spanning ...
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bioRxiv - Plant Biology 2023Quote: ... The ICP multi-element standard solution Intelliquant No.1 and 2 from Agilent were used for calibration using following concentrations ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were incubated with polyclonal goat anti-mouse FITC (DAKO F0479 1:20). Finally ...
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bioRxiv - Evolutionary Biology 2023Quote: ... DNA quality was assessed by running 1 μL on the Bioanalyzer system (Agilent) to ensure size and analysis of DNA fragments ...
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bioRxiv - Immunology 2023Quote: ... antirabbit or anti-mouse HRP-conjugated antibodies (1:2000; Dako, Carpinteria, CA, USA) were used for detection of the primary antibodies ...
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bioRxiv - Immunology 2023Quote: ... the slides were washed with Gene Expression Wash Buffers 1 and 2 (Agilent) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were incubated for 1 h in unbuffered XF assay media (Agilent Technologies) supplemented sequentially with either 2 mM glutamine ...
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bioRxiv - Immunology 2023Quote: ... diluted in DAKO antibody diluent (1:100) with background reducing components (DAKO, S3022). For DAB staining ...
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bioRxiv - Cell Biology 2024Quote: ... Ligated plasmids were transformed into competent XL-1 blue cells (Agilent Technologies #200249). I440A and R479A MDM2 mutants were generated by site-directed mutagenesis ...
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bioRxiv - Immunology 2023Quote: ... Viral foci were detected using 1:10,000 dilution of αHSV rabbit antibody (Dako) and 1:50,000 dilution of goat αrabbit HRP conjugated antibody ...
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bioRxiv - Molecular Biology 2023Quote: ... and the secondary polyclonal swine anti-rabbit immunoglobulins/HRP (1:10.000, DAKO P0399) and polyclonal rabbit anti-mouse immunoglobulins/HRP (1:10.000 ...
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bioRxiv - Cell Biology 2023Quote: ... The following primary antibodies were used: gp-insulin (Agilent/DAKO IR002, 1:2), mo-glucagon (Sigma-Aldrich #G2654 ...
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bioRxiv - Cell Biology 2023Quote: ... The following primary antibodies were used: gp-insulin (Agilent/DAKO IR002, 1:2), mo-glucagon (Sigma-Aldrich #G2654 ...
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bioRxiv - Molecular Biology 2023Quote: ... with 1 µL pooled library and running in D1000 ScreenTape (Agilent 5067-5582). Libraries were sequenced on the Illumina NovaSeq 6000 or NovaSeq X+ platforms.
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bioRxiv - Physiology 2024Quote: ... Anti-rabbit coupled to HRP was used as secondary antibodies (1:2000, Dako). Signals were revealed by chemiluminescence (Thermo Fischer ...
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bioRxiv - Genomics 2024Quote: ... 87.5 μl of 10 mg ml−1 boiled salmon sperm DNA (Agilent Genomics) was added to each tube with 1.75 μg of plasmid library ...
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bioRxiv - Molecular Biology 2024Quote: ... The membranes were incubated with an anti-goat (Agilent, Cat# P0160, 1:5000) or anti-rabbit immunoglobulin/HRP secondary antibody (Agilent ...
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bioRxiv - Neuroscience 2024Quote: ... Proliferating cells were nuclear labeled with the Ki67 antibody (1:200, Dako GA626). The primary antibody binding was visualized using a biotinylated secondary antibody followed by developing with avidin-peroxidase and diaminobenzidine or 3-amino-9-ethylcarbazole.
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated overnight with primary antibodies GFAP (DAKO, Z0334 1 : 500, RRID:AB_10013482), p-cJUN (Cell Signaling 9261 1:500 ...
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bioRxiv - Cancer Biology 2024Quote: ... FFPE sections were stained with Ki67 primary antibody (Clone MIB-1, DAKO, Denmark) (1:200 ...
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bioRxiv - Molecular Biology 2024Quote: ... Immunohistochemistry was performed using antibody against CD20 (clone L26, Dako, dilution 1:2400) by automated IHC stainer (Ventana BenchMark ULTRA ...
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bioRxiv - Plant Biology 2020Quote: ... RNA quality and concentration of each sample were checked using an Agilent RNA 6000 Nano Kit Bioanalyzer chip (Agilent Technologies). Five micrograms of total purified RNA was enough for the reverse transcription reactions using the SuperScript III First-Strand Synthesis System for RT-PCR (Life Technologies) ...
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bioRxiv - Microbiology 2020Quote: ... Site-directed mutagenesis of the pCMV-S4 vector was performed using protocols and materials from QuickChange Lightning mutagenesis kit (Agilent). Nearly confluent monolayers of HEK293 cells in 12 well plates were transfected with either 0.5 μg of empty vector or 0.25 μg each of WT σ3 or σ3 K293T expression vector using 1.5 µl Lipofectamine 2000 according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: Genes encoding EnHD and CHD in pET24 expression plasmids were described previously.(18) Single residue substitutions were introduced using a Quikchange site-directed mutagenesis kit (Agilent). Genes encoding sequences with multiple substitutions were synthesized by GeneArt (ThermoFisher Scientific ...
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bioRxiv - Genetics 2020Quote: Plamids for overexpression of the Nrd1 CID variants R133G and R133D were obtained using QuikChange site-directed mutagenesis kit (Stratagene).
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bioRxiv - Cell Biology 2021Quote: ... Hsc70D10N was generated by PCR-based site-directed mutagenesis using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies). Cyclin D1 KO was conducted by CRISPR/Cas9 genome editing (Xie et al. ...
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bioRxiv - Systems Biology 2020Quote: ... The resulting pure DNA and RNA fractions were quality assessed using agarose gel electrophoresis and Bioanalyser RNA 6000 Pico Kit (Agilent). Nucleic acid concentration was quantified using Qubit dsDNA HS Assay and Qubit RNA HS Assay Kit (Invitrogen) ...
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bioRxiv - Molecular Biology 2020Quote: The lyase deficient mutant of HMGA2 was generated by sequential site-directed mutagenesis (QuikChange II Site-Directed Mutagenesis Kit, Agilent) of the first three arginines in the third AT-Hook domain ...
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bioRxiv - Molecular Biology 2020Quote: ... plasmid by digestion with AscI and MIuI and mutagenesis for RTEL1K48R was performed using QuikChange Lightning Multi Site-Directed Mutagenesis Kit (Agilent). Cells were infected with lentiviruses expressing the indicated RTEL1 proteins and infected cells were selected with puromycin as previously described (Garzia et al. ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples quantity (RNA Integrity Number = RIN) and quality were assessed using the Agilent RNA 6000 Pico Kit (Agilent #5067-1513) on the Bioanalyzer 2100 ...
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bioRxiv - Cell Biology 2020Quote: ... The pcDNA3-HA-Bbs5-S19A construct was prepared by mutating Ser19 of Bbs5 to alanine with pcDNA-HA-Bbs5-WT as template and site-directed mutagenesis kit (Stratagene). The primers were FWD1 (5′-CTTCGACGTGTCCGCACAACAGATGAAAAC-3′ ...
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bioRxiv - Cell Biology 2020Quote: ... Site directed mutagenesis was done using a QuikChange® Lightning Site Directed Mutagenesis kit (Agilent Technologies, Inc., Santa Clara, CA).
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bioRxiv - Cell Biology 2020Quote: Mito fuel flex test: Agilent Seahorse XF Mito Fuel Flex Test Kit was used as per the standard protocol by Agilent Seahorse (https://www.agilent.com/cs/library/usermanuals/public/XF_Mito_Fuel_Flex_Test_Kit_User_Guide%20old.pdf) ...
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bioRxiv - Cell Biology 2020Quote: ... mRNA were extracted and the quality of these samples was assessed (Agilent RNA 6000 Nano Kit Quick, Agilent Bioanalyzer 2100); RNA Integrity Number (RIN ...
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bioRxiv - Cell Biology 2020Quote: A library encoding ARHGAP36 isoform 2 mutants was created via error-prone PCR (epPCR) using the GeneMorph II Random Mutagenesis Kit (Agilent). To determine the optimal epPCR conditions for library generation ...
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bioRxiv - Cell Biology 2020Quote: ... pWZL-Hygro-HDAC4PAM-ER pCW-Hygro-HDAC4PAM were obtained by sub-cloning a mutagenized HDAC4 (QuikChange Site-Directed Mutagenesis Kit, Agilent) into the linearized empty backbones by a restriction-based approach ...
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bioRxiv - Cell Biology 2019Quote: ... Point mutations were made with complementary primers (listed below) in pTRCHisA-padi2 vectors using QuikChange II Site-Directed Mutagenesis Kit (Agilent).
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bioRxiv - Cell Biology 2020Quote: ... pEGFP-C1-tubbyCT R332H and pEGFP-C1-tubbyCT Y343F were generated using QuikChange II XL Site-Directed mutagenesis kit (Stratagene, Agilent Technologies ...
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bioRxiv - Immunology 2021Quote: ... RNA integrity was determined by bleach agarose gel electrophoresis (Aranda et al., 2012) and further analyzed on a Bioanalyzer 2100 using the RNA 6000 Nano LabChip Kit (Agilent Technologies Inc. ...
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bioRxiv - Immunology 2021Quote: ... and extracellular acidification rate (ECAR) were measured in MSCs using a Seahorse XF24 Extracellular Flux Analyzer and kits from Agilent Technologies (Santa Clara ...
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bioRxiv - Immunology 2021Quote: ... PCR products were ligated into pSCB-Amp/Kan using the StrataClone Blunt PCR Cloning Kit (Agilent Technologies, La Jolla, CA). The Spic promoter was cloned into pGL3-Basic (Promega ...
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bioRxiv - Genetics 2021Quote: ... Quality assessment and library size estimation of the individual libraries was done using an HS DNA kit (Agilent, #5067-4626) in a Bioanalyzer 2100 ...
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bioRxiv - Developmental Biology 2021Quote: RNA integrity was assessed on an Experion bioanalyzer using a Bio-Rad Experion RNA High-Sensitivity kit or an Agilent 2100 bioanalyzer using a Bioanalyzer RNA 6000 Pico assay (Agilent). RNA concentrations were measured using a Qubit 4 fluorometer with a Qubit high-sensitivity RNA assay.
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bioRxiv - Developmental Biology 2021Quote: ... or the Illumina TruSeq Stranded Total RNA Library Prep kit (reripes samples) and checked using a Fragment Analyzer System (Agilent) before multiplexing ...
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bioRxiv - Molecular Biology 2021Quote: ... after which quantification and qualification of purified band(s) were undertaken using the Agilent DNA 1000 kit and BioAnalyzer (Agilent). Illumina NGS was performed with 2×250 bp sequencing without fragmenting the amplicons ...
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bioRxiv - Evolutionary Biology 2020Quote: ... RNA probes were synthetized by cloning the DNA amplified region in the Strataclone PCR Cloning Kit (240205-5, Agilent Technologies) (hoxd12a ...
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bioRxiv - Biophysics 2020Quote: ... by introducing a stop codon at position 475 (aa 233–474) using the QuikChange XL site-directed mutagenesis kit (Stratagene). STIM1 fragment 344-449 (CAD ...
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bioRxiv - Developmental Biology 2020Quote: ... RNA integrity was assessed through the RNA 6000 Pico Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). A total amount of 3μg RNA per sample was used for mRNA purification and library construction via the Truseq™ RNA Sample Prep Kit (Illumina ...
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bioRxiv - Developmental Biology 2020Quote: ... Size distribution and concentration of the final products were then evaluated using the Bioanalyzer High Sensitivity DNA kit (Agilent Technologies). The libraries were pooled and diluted to 3 nM using 10 mM Tris-HCl ...
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bioRxiv - Genomics 2020Quote: ... and RNA 6000 Pico and RNA 6000 Nano kits –depending on the experiment– with a BioAnalyzer 2100 (Agilent Technologies Inc.). Libraries for each sample type were prepared using the TruSeq Stranded Total RNA Library Prep Kit (Illumina) ...