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Citations for Agilent :
5551 - 5600 of 8383 citations for Human Adenosylhomocysteinase Like 1 AHCYL1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... SIK3-Flag-S884A and S884D plasmids were generated by introducing site-directed mutations into SIK3-Flag plasmid using the QuikChange II XL site-directed mutagenesis kit according to manufacturer’s instruction (Agilent). Lenti-Puro-SIK3-Flag plasmid was constructed by subcloning the “mSIK3-3xFlag” coding sequences (CDS ...
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bioRxiv - Cell Biology 2023Quote: ... Sequencing libraries were validated using NGS Kit on the Agilent 5300 Fragment Analyzer (Agilent Technologies, Palo Alto, CA, USA), and quantified by using Qubit 4.0 Fluorometer (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... The C121S ΔPTPN1 mutant was generated from the ΔPTPN1 vector using a QuikChange II Site Directed Mutagenesis kit (Agilent), and the mutant sequence verified by whole plasmid sequencing (Azenta) ...
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bioRxiv - Genomics 2023Quote: ... Sequencing libraries were validated using DNA Kit on the Agilent 5600 Fragment Analyzer (Agilent Technologies, Palo Alto, CA, USA), and quantified by using Qubit 4.0 Fluorometer (Invitrogen ...
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bioRxiv - Genomics 2023Quote: ... and RNA integrity was checked with RNA Kit on Agilent 5600 Fragment Analyzer (Agilent Technologies, Palo Alto, CA, USA).
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bioRxiv - Cell Biology 2023Quote: ... Mutations were inserted by site-directed mutagenesis using the QuikChange Lightning site-directed mutagenesis kit (Agilent Technologies, CA, USA) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... PD-L1 TPS was determined using the Dako PD-L1 IHC 22C3 pharmDx kit (Agilent Technologies, Santa Clara, CA), which is approved by the US Food and Drug Administration.
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bioRxiv - Cell Biology 2023Quote: ... The DNA libraries were constructed using TruePrep DNA Library Prep Kit V2 for Illumina (TD503-02, Vazyme) following the manufacturer’s instructions and quantified by Agilent Bioanalyzer 2100 system ...
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bioRxiv - Genomics 2023Quote: ... Fragment size was controlled by capillary electrophoresis with the DNA high sensitivity kit and a Bioanalyzer 2100 (Agilent Technologies). DNA ends were repaired using the End-It DNA End-Repair Kit (Epicentre) ...
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bioRxiv - Neuroscience 2023Quote: EEF1A2 mutations in both the CMV-NGFP-EEF1A2-Neo vector and the pcDNA3.1-CMV-Flag-EEF1A2 vector were generated using the QuikChange II site directed mutagenesis kit (Agilent) according to the manufacturers protocol using the following primers.
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bioRxiv - Molecular Biology 2023Quote: All mutagenesis reactions were carried out using the QuikChange II Site-Directed or Lightning MultiSite-Directed Mutagenesis Kits (Agilent) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The pHD1344tub(PAC)-Nterm3V5-A3 plasmid was mutagenized using the QuickChange Lightning site-directed mutagenesis kit (Agilent, product #210518) and primers listed in Table S2 ...
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bioRxiv - Pathology 2023Quote: ... three clusters of lysine- and arginine-residues and one cluster of asparagine residues were exchanged for alanine within mouse Prnp in a pcDNA3.1 vector by site-directed mutagenesis (QuikChange site-directed mutagenesis kit; Agilent). PrP-deficient RK13 cells (ATCC ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated in the secondary antibody-horseradish peroxidase (HRP) complex from the REAL EnVision detection kit (Dako #K5007) for 1h at RT ...
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bioRxiv - Immunology 2023Quote: ... site-directed mutagenesis using specific primers was performed based on the QuikChange site-directed mutagenesis Kit II (Agilent Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... The concentration and quality of RNA samples from day 70 was assessed using a Bioanalyzer RNA kit (Agilent Technologies). Total RNA was prepped with the NuGEN Universal with AnyDeplete Human Ribo kit from ∼100 ng of total RNA input ...
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bioRxiv - Biochemistry 2023Quote: ... Library quality was assessed using an Agilent Bioanalyzer 2100 and the Agilent-1000 DNA kit (Agilent Cat# 5067-1504), and libraries were quantified using the Qubit dsDNA Quantitation ...
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bioRxiv - Biochemistry 2023Quote: ... The resulting PCR products were subcloned into the holding vector pSC-B (StrataClone Blunt PCR Cloning Kit, Agilent Technologies) and 16 colonies (white ...
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bioRxiv - Biochemistry 2023Quote: ... For generation of single point LIR mutants and ΔLIR BAG3 the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent, 210518) was utilized according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... Concentration and quality of the synthesised cDNA were assessed using the Bioanalyzer High-Sensitivity DNA Kit (Agilent, 5067-4627). cDNA was successfully amplified from the suspension containing 8,000 nuclei however cDNA prepared from the suspension containing 8,000 cells was undetectable and therefore library preparation was discontinued at this step ...
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bioRxiv - Cell Biology 2023Quote: ... and RNA integrity was checked with RNA Kit on Agilent 5300 Fragment Analyzer (Agilent Technologies, Palo Alto, CA, USA). RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina following the manufacturer’s instructions (NEB ...
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bioRxiv - Cell Biology 2023Quote: ... Sequencing libraries were validated using NGS Kit on the Agilent 5300 Fragment Analyzer (Agilent Technologies, Palo Alto, CA, USA), and quantified by using Qubit 4.0 Fluorometer (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... 2.2 kb) 20 was labelled by [a-32P]-dCTP by Prime-It II Random Primer Labeling Kit (Agilent Technologies) and hybridized with Hybond-N ...
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bioRxiv - Immunology 2023Quote: ... SAMD9L-F886Lfs*11-E198A/D243A were generated from pMAX:mTFP1-SAMD9L plasmid using the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... λgt10 phage vectors in genomic DNA were excised using commercial packaging extract kits (Agilent Technology, Santa Clara, CA, USA), according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... and fragment size distribution with the Agilent® High Sensitivity DNA Kit (Agilent Technologies, Inc., Cat. No. 5067-4626). Libraries were sequenced as paired-end 150×150bp on a NovaSeq® 6000 System (Illumina ...
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bioRxiv - Genomics 2023Quote: ... and quality was determined with a DNA ScreenTape Kit on the TapeStation 2200 system (Agilent; 5067-5582, 5067-5583). Sequencing was done using a HiSeq 2500 (Illumina).
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bioRxiv - Cell Biology 2023Quote: ... Amplified cDNA and final libraries were evaluated on an Agilent BioAnalyzer using a High Sensitivity DNA Kit (Agilent Technologies). Individual libraries were diluted to 4nM and pooled for sequencing ...
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bioRxiv - Developmental Biology 2023Quote: ... PCR amplicons were further cloned into a pSC-amp/kan vector using StrataClone PCR cloning kit (240205, Agilent Technologies). We picked ∼20-50 single colonies per sample to determine the presence ...
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bioRxiv - Genomics 2023Quote: ... and size distribution was determined using the high sensitivity DNA kit on the Bioanalyzer 2100 (Agilent, Santa Clara, CA). Pooled libraries were sequenced on the Illumina NovaSeq (paired-end 150 ...
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bioRxiv - Biochemistry 2022Quote: AtCOSY mutants were generated according to the protocol described in the QuickChange II Site-Directed Mutagenesis Kit (Agilent Technologies) using plasmid pHis8-4b::AtCOSY as the template and the primer sequences in Supplementary Table 5 ...
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bioRxiv - Neuroscience 2023Quote: ... RNA quality was assessed using an Agilent 2100 bio-analyzer and the RNA 6000 pico kit (Agilent 5067-1513). Next ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The quantity and quality of extracted RNA samples were analyzed using RNA 6000 Nano kit on a bioanalyzer (Agilent). RNA samples with RNA integrity number (RIN)>7 were used for library construction using the 3′ mRNA-Seq Library Prep Kit FWD for Illumina (QuantSeq-LEXOGEN ...
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bioRxiv - Cell Biology 2023Quote: ... Sequencing libraries were validated using NGS Kit on the Agilent 5300 Fragment Analyzer (Agilent Technologies, Palo Alto, CA, USA) and quantified by using Qubit 4.0 Fluorometer (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... and RNA integrity was checked with RNA Kit on Agilent 5300 Fragment Analyzer (Agilent Technologies, Palo Alto, CA, USA). RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina following manufacturer’s instructions (NEB ...
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bioRxiv - Physiology 2023Quote: ... followed by Vectastain Elite ABC HRP detection kit (Vector PK-6100) and detection with DAB (Agilent Technologies K346811-2). Cell death and DNA fragmentation were detected with a TUNEL In Situ Cell Death Detection Kit (Roche ...
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bioRxiv - Microbiology 2023Quote: ... The TEAD-binding defective HPV8 E6 K136N mutant was made using the QuikChange II site-directed mutagenesis kit (Agilent). Nuclear/cytoplasmic fractionation was performed using the previously published Rapid ...
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bioRxiv - Neuroscience 2023Quote: ... Quantification and quality checked of libraries was done using an Bioanalyzer 2100 instrument and DNA 7500 kit (Agilent Technologies). Libraries were pooled and sequenced in two lanes on a HiSeq 2500 ...
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bioRxiv - Cell Biology 2023Quote: ... The α10-N839Q mutation was generated by QuickChange mutagenesis kit following the instruction (cat# 200517-5, Agilent Technologies, Inc.). All DNA constructs were identified by sanger sequencing service provided by Retrogen ...
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bioRxiv - Systems Biology 2024Quote: ... targeted DNAs were captured and enriched by SureSelect XT HS Target Enrichment Kit ILM Hyb Module according to the manufacturer’s instructions (Agilent). Indexed libraries were quantitated by high sensitivity DNA ScreenTape assay for TapeStation (Agilent) ...
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bioRxiv - Developmental Biology 2024Quote: ... Library fragment size was assessed using Agilent Bioanalyzer 2100 with the High Sensitivity DNA analysis kit (Agilent, 5067-4626) and concentration was determined using Qubit™ dsDNA HS Assay kit (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR products were assessed for purity and concentration on the Bioanalyzer using the High Sensitivity DNA Kit (Agilent Technologies). Samples were multiplexed and sequenced on the Illumina HiSeq 2500 system (Genome Technology Core ...
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bioRxiv - Neuroscience 2023Quote: ... The fragment size of the final libraries was determined using the Bioanalyzer High-Sensitivity DNA Kit (Agilent, CA, USA). Library concentration was determined using the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... RNA integrity was assessed on an Agilent 2100 Bioanalyzer using the RNA 6000 nano kit (Agilent, Santa Clara, CA) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The average library size for the pool was determined using an Agilent DNA D1000 Tape Station kit (Agilent #5067).
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bioRxiv - Biochemistry 2023Quote: ... Gly241Glu and Asp235Ala:Gly241Glu were introduced into the previously published vectors (5) using the QuickChange II Site-directed mutagenesis kit (Agilent) using primers listed in Table S2.
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bioRxiv - Biochemistry 2023Quote: ... The plasmid carrying the WT Cagα protein pHTCagα was amplified using the QuikChange II site-directed mutagenesis kit (Agilent) using 33 bp primers (suppl ...
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bioRxiv - Biophysics 2023Quote: ... The Pro283Ser amino substitution was introduced to this vector via the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent) as previously described (13) ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... was used for the RNA quality control with a microfluidic chip specific for RNA (Agilent RNA 6000 Nano Kit). Then 1 μg of qualified RNA samples in RNase-free ddH2O was used for library preparation ...
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bioRxiv - Biochemistry 2023Quote: The F32A mutants of WT and Δ20-ACA8 were created using a QuikChange site directed mutagenesis kit (Agilent technologies) and the following primers: