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Citations for Agilent :
501 - 550 of 8233 citations for Human Adenosylhomocysteinase Like 1 AHCYL1 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... Point mutations (I91F and K176E) were introduced using the QuickChange II XL Site-Directed Mutagenesis Kit (SI Table 1; Agilent Technologies). The full-length sequence of the PAI-1 constructs was confirmed by Sanger sequencing (SI Table 1) ...
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bioRxiv - Cancer Biology 2023Quote: ... and RT-qPCR was performed using the 1-Step Brilliant II SYBR Green quantitative RT-PCR master mix kit (Agilent Technologies) on a Bio-Rad C1000 Thermal Cycler CFX96 Real-Time System ...
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bioRxiv - Molecular Biology 2024Quote: The ATG (+1) codon of pKL-RLBP1 was mutated to an alanine codon (GCG) using the QuikChange Site-directed Mutagenesis kit (Agilent technologies) with the F primer and its reverse complement (Table S1 ...
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bioRxiv - Cancer Biology 2024Quote: ... oligomycin (1 µM) and 2-deoxy-D-glucose (2-DG) (50 mM) (Seahorse Glycolysis Stress Test Kit, Agilent Technologies, 103020-100). Analyses were performed with Wave software 2.3.0 ...
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bioRxiv - Cancer Biology 2021Quote: ... 0.75 μg labeled cRNA was fragmented and hybridized to custom whole-genome human 8 × 60K microarrays (Agilent-048908) according to the supplier’s protocol (Agilent Technologies) ...
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bioRxiv - Pathology 2022Quote: ... the hydrogels were incubated overnight with a mouse anti-human α-smooth muscle actin antibody (DAKO, Glostrup, Denmark) at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... and 90 of human ATG12 were mutagenized into arginine using site-directed mutagenesis following the manufacturer’s instructions (Agilent Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... target enrichment was performed using the Agilent SureSelect Human All Exon V6 design (Agilent, Santa Clara, CA, USA).
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bioRxiv - Cancer Biology 2021Quote: ... Genomic DNA of tumor and matched germline samples were captured using the SureSelect Human All Exon v7 (Agilent) or xGen Exome Research Panel v2 (IDT) ...
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bioRxiv - Biochemistry 2022Quote: Aliquots of 20 µL of human plasma were depleted on a 1260 Infinity Bio-inert LC system (Agilent) coupled to a depletion column (human 14 multiple affinity removal column ...
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bioRxiv - Microbiology 2021Quote: ... Oligonucleotide microarrays for human whole genome (G4858A design 072363, 8×60k chips SurePrint G3 unrestricted GE, Agilent Technologies) were used for global gene expression analysis ...
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bioRxiv - Biochemistry 2020Quote: ... 2009). MEKK1 542–888 construct (H. sapiens MEKK1 TOG) was amplified from the MegaMan human transcriptome library (Agilent). The TOG construct was cloned into pETNKI-his-3C-LIC-kan vector ...
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bioRxiv - Cancer Biology 2021Quote: ... The labeled complementary RNAs were hybridized onto a whole human genome oligo microarray (4 3 44K; Agilent Technologies). After washing the slides ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed and incubated with horse radish peroxidase (HRP) conjugated goat-anti-human IgG antibody (Dako, P0447) (diluted 1:1,600 in PBS/0/1% BSA ...
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bioRxiv - Microbiology 2023Quote: ... rabbit F(ab’)2 anti-human C1q-FITC (both at 5 µg/ml, Dako as described in (9)) ...
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bioRxiv - Cancer Biology 2022Quote: A premade human placenta plasmid cDNA library in the pCFB XR vector (ViraPort XR) was purchased from Stratagene. Retroviruses were produced in 293T cells followed by infection of target 293T cells according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Positive control PhIP-seq designed to capture mouse and human GFAP began by incubating 0.1µg/mL polyclonal anti-GFAP antibody (Dako) with the murine PhIP-seq library.
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bioRxiv - Immunology 2023Quote: ... the primary polyclonal rabbit anti-human/mouse fibrin(ogen) (10 μg/mL in HBSS, Agilent Dako, Glostrup, DK) antibody was added overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... the primary polyclonal rabbit anti-human/mouse fibrin(ogen) (10 μg/mL in HBSS, Agilent Dako, Glostrup, DK) antibody was added overnight at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... which recognises residues 1978-2016 of human NaV1.5) and stained with EnVision+ Dual Link System/HRP (DAB+; Dako), following the manufacturer’s instructions and counterstained with Mayer’s haematoxylin ...
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bioRxiv - Immunology 2024Quote: ... RcγRIV (generated by the Duke Human Vaccine Institute) and conjugating the biotinylated FcγR with Streptavidin-PE (Prozyme PJ31S)63 ...
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bioRxiv - Cancer Biology 2024Quote: ... pooled (8-plex) in an equimolar fashion and hybridized overnight with SureSelect Human All Exon V5 baits (Agilent). The multiplexed samples were paired-end sequenced using the NovaSeq 6000 platform (Illumina ...
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bioRxiv - Genomics 2021Quote: ... high-sensitivity DNA kits and RNA pico kits (Agilent, Tokyo, Japan) were used ...
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bioRxiv - Molecular Biology 2024Quote: ... with dsDNA High Sensitivity Assay Kit and Bioanalyzer 12000 kit (Agilent). Binding was performed with the Sequel II Binding Kit 3.1 ...
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bioRxiv - Bioengineering 2021Quote: ... pCS2-GemininΔ27-GFP was generated by deleting amino acids 1-27 from Geminin-GFP [27] using QuikChange® Lightning mutagenesis kit (Agilent, 210513). The plasmids pCS2-Flag-FA-Geminin-GFP w.t and Δ27 variant were generated by cloning Geminin-GFP into pCS2-Flag-FA vector sing FseI and AscI restriction sites ...
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bioRxiv - Developmental Biology 2023Quote: Whole-mount immunostaining of differentiated skeletal muscle cells was performed using the monoclonal hybridoma 12/101 primary antibody [38] (1/200 dilution; DSHB #AB-531892) and the EnVision+ Mouse HRP kit (Agilent Technologies, K4007) according to the manufacturer’s recommendations.
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bioRxiv - Neuroscience 2023Quote: ... followed by site-directed mutagenesis at Cys322 site to Serine using QuikChange Site-directed mutagenesis kit (Agilent, Santa Clara, CA; Fig. 1). 4RCF construct (four microtubule-binding repeats and cysteine-free construct containing C291S and C322S mutations ...
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bioRxiv - Biochemistry 2022Quote: ... The phPAI-1 library was generated by error prone PCR using the GeneMorph II Random Mutagenesis Kit (Agilent Technologies, Santa Clara, CA) with primers that maintain the AscI and NotI restriction sites (SI Table 1) ...
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bioRxiv - Biophysics 2023Quote: ... the following HIV-1 IN mutants were prepared using the QuikChange II XL site directed mutagenesis kit (Agilent Technologies, Santa Clara, CA): E138K ...
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bioRxiv - Systems Biology 2024Quote: ... Average dsDNA library size distribution was determined using the Agilent HS NGS Fragment (1-6000 bp) kit on the Agilent Fragment Analyzer (Agilent Technologies, USA). Libraries were normalized and pooled in the normalization buffer (10 mM Tris-Cl ...
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bioRxiv - Cell Biology 2020Quote: Gene expression analysis were performed with Agilent® SurePrint G3 Human GE 8×60K Microarray (Agilent Technologies, AMADID 39494) with the following dual-color design ...
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bioRxiv - Genetics 2021Quote: Oxford Nanopore MinION long-read sequencing was performed on RNA isolated from an adult human cortex (540005, Agilent Technologies). PolyA-enriched RNA was isolated using the Dynabeads mRNA Direct kit (Life Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... Exome-sequencing libraries were generated using the Agilent SureSelect Human All Exon V4+UTR capture platform (Agilent, CA, USA). Libraries were sequenced to a target depth of 200-fold coverage on a 100 bp paired-end sequencing run using an Illumina HiSeq 2000 instrument ...
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bioRxiv - Neuroscience 2021Quote: Oxygen Consumption Rate (OCR) from live human iPSC-derived neurons was measured using a Seahorse XFe-96 Analyzer (Agilent). 30,000 human pre-differentiated neurons were seeded per well in Poly-D-Lysine + Laminin-coated 96-well Seahorse plate and differentiated for the indicated time (2 or 4 weeks ...
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bioRxiv - Cancer Biology 2022Quote: The tumor and normal adjacent samples were prepared using SureSelect Human All Exon V7 library (Agilent, Santa Clara USA). 100 bp paired end reads sequencing was performed using the Illumina NovaSeq 6000 system by Edinburgh Genomics (Edinburgh ...
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bioRxiv - Neuroscience 2021Quote: ... Mamu-DR (monoclonal mouse – anti-human HLA-DR/DQ-IgG1, clone CR3/43, cat. no. M077501-2, Agilent Technologies), 1:150 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... SCA-associated mutations were introduced into human cMyc-mGlu1 using Quikchange site-directed mutagenesis (Agilent Technologies, Santa Clara, CA). Mutagenesis primers are shown in Table S1 ...
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bioRxiv - Cancer Biology 2022Quote: ... The following antibodies were used: AMACR/p63 immunohistochemistry (IHC) double staining (Monoclonal Rabbit Anti-Human AMACR, clone 13H4, Dako, Code M361601-2 ...
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bioRxiv - Immunology 2024Quote: ... Microarray analysis was performed at TaKaRa-Bio using high-density oligonucleotide array (Human Genome U133 Plus 2.0) and GeneSpring 12.5 software (Agilent Technologies) as described previously (17) ...
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bioRxiv - Cancer Biology 2022Quote: ... tumor mutational burdens were computed using the exonic ranges from the SureSelect Human All Exon v7 panel from Agilent (bed file downloaded from the manufacturer’s website ...
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bioRxiv - Developmental Biology 2024Quote: ... at a density of 9.1 × 105 cells/cm2 (mouse assay) or 7.3 × 105 cells/cm2 (human assay) in 100 μl of Seahorse XF DMEM (Agilent) supplemented with glucose (20 mM) ...
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bioRxiv - Immunology 2024Quote: The tumor and normal adjacent samples were prepared using SureSelect Human All Exon V7 library (Agilent, Santa Clara USA). 100 bp paired end reads sequencing was performed using the Illumina NovaSeq 6000 system by Edinburgh Genomics (Edinburgh ...
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bioRxiv - Immunology 2024Quote: ... Sections were incubated overnight at 4°C with 10 µg/ml polyclonal rabbit anti-human/mouse fibrin(ogen) (Dako), 5 µg/ml rat anti-mouse C3b/iC3b (clone 3/26 ...
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bioRxiv - Cancer Biology 2024Quote: Gene expression profiles for queried cells were analyzed using Agilent Human 44K expression arrays (Agilent Techonologies, Santa Clara, CA). Data was loess-normalized to correct for dye and ratio bias at different signal intensities ...
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bioRxiv - Biophysics 2024Quote: Human wild-type α-synuclein and A90C cysteine variants were purified from Escherichia coli BL21 (DE3)-gold (Agilent Technologies) expressing plasmid pT7-7 encoding for α-synuclein as previously described35,42,43 ...
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bioRxiv - Cancer Biology 2024Quote: ... pooled (8-plex) in an equimolar fashion and hybridized overnight with the SureSelect Human All Exon V5 baits (Agilent). The multiplexed samples were paired-end sequenced using the NovaSeq 6000 platform (Illumina ...
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bioRxiv - Pathology 2022Quote: ... using a kit assay (Agilent RNA 6000 Nano Kit 5067-1511, Agilent).
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bioRxiv - Pathology 2022Quote: ... using a kit assay (Agilent RNA 6000 Nano Kit 5067-1511, Agilent).
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bioRxiv - Neuroscience 2021Quote: ... the LSAB2 Kit (Dako) for rabbit/mouse and the DAB-Kit from Zytomed was used according to the manufacturers’ protocols ...
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bioRxiv - Biophysics 2022Quote: The QuickChangeTM kit (Stratagene) and Pfu Turbo DNA polymerase were applied for nucleotide mutagenesis in pASK-IBA5betP [4] and pASK IBA7-betPΔN29-EEE44/45/46AAA [14] plasmids ...