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Citations for Agilent :
451 - 500 of 8233 citations for Human Adenosylhomocysteinase Like 1 AHCYL1 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... miRNA expression was measured using the Human miRNA Microarray Slide (Release 19.0) with Design ID 046064 (Agilent Technologies ...
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bioRxiv - Cancer Biology 2021Quote: ... total RNA was isolated from human normal and tumor tissues according to manufacture protocol (Agilent Cat#400800), and the quantity and quality were confirmed by NanoDrop 2000C spectrophotometer ...
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bioRxiv - Neuroscience 2021Quote: Target enrichment made use of the SureSelectTX human all-exon library (V6, 58 mega base pairs; Agilent) and high-throughput sequencing was carried out using a HiSeq4,000 (2×75-base-pair paired-end configuration ...
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bioRxiv - Neuroscience 2021Quote: Target enrichment made use of the SureSelectTX human all-exon library (V6, 58 mega base pairs; Agilent) and high-throughput sequencing was carried out using a HiSeq4,000 (2×75-base-pair paired-end configuration ...
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bioRxiv - Molecular Biology 2020Quote: ... then sent to the Plateforme Génomique de Nantes for hybridization on SurePrint G3 human exon microarrays (Agilent). Raw signals were LOWESS-normalised before analysis ...
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bioRxiv - Cancer Biology 2022Quote: Comparative genome hybridization was performed using the Agilent SurePrint G3 Human CGH 60 K microarray (Agilent Technologies) spanning the entire human genome at a median resolution of 41 kb ...
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bioRxiv - Molecular Biology 2022Quote: contains the canonical human HELZ2 long isoform cDNA (2649 bps) cloned into pCMV-3Tag-9 (Agilent Technologies), which allows the expression of a HELZ2-3xMYC fusion protein ...
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bioRxiv - Cell Biology 2024Quote: ... The following primary antibodies were used: anti-keratin 20 (KS.20.8, mouse monoclonal anti-human; Agilient-Dako), anti-PCNA (PC-10 ...
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bioRxiv - Neuroscience 2023Quote: ... rAAVs were produced by co-transfection of human embryonic kidney (HEK) cell line 293 (Stratagene, California, USA) with the target AAV plasmid and helper plasmids (pFΔ6 ...
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bioRxiv - Neuroscience 2024Quote: Whole-exome sequencing target enrichment used the SureSelectTX human all-exon library (V6, 58 megabase pairs; Agilent) and high-throughput sequencing was carried out using a HiSeq 4,000 (sx75 base-pair paired-end configuration ...
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bioRxiv - Neuroscience 2024Quote: ... mouse or human brain cryosections (30 μm) were treated with serum-free protein blocking solution (X0909, Dako). The tissues were incubated with the following primary antibodies overnight at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... MDMs were then incubated with Alexa Flour 647 anti-human CD169 antibody in Dako antibody diluent (Dako) overnight at 4° ...
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bioRxiv - Cancer Biology 2024Quote: ... Gene expression levels generated from independent runs were normalized to universal human reference RNA (UHRR) standard (Stratagene), a positive control composed of total RNA from 10 human cell lines used as a constant reference in gene-profiling analyses.
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bioRxiv - Genetics 2024Quote: ... The target regions were captured using SureSelectXT Human All Exon V5 (Agilent Technologies, Santa Clara, CA, USA) or CapTruth Human Exome V2.0 (Medical Laboratory of Nantong ZhongKe Co. ...
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bioRxiv - Molecular Biology 2021Quote: ... HRP-conjugated secondary antibodies were then incubated on slides for 1-hour and detected with the DAB+ chromogen kit (DAKO). After washing ...
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bioRxiv - Biochemistry 2020Quote: ... fulgidus Csx3 (Supplementary figure 1) were generated using the QuikChange Site-Directed Mutagenesis kit as per manufacturer’s instructions (Agilent Technologies) and purified as for the wild-type protein.
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bioRxiv - Microbiology 2020Quote: ... an optimal mutation rate (0.3–1 base/kb) for 1µg of template was adopted as recommended in GeneMorph II Random Mutagenesis kit (Agilent Technologies). The PCR products were then digested with EcoRI and HindIII and ligated into the pJF118EH vector using the same restriction enzymes ...
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bioRxiv - Molecular Biology 2020Quote: ... were introduced using RE specific primers (Table 1 and Table 2 in supplementary material) by QuickChange Multi site-directed mutagenesis kit (Agilent) according to manufacturer’s instructions.
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bioRxiv - Neuroscience 2020Quote: ... An AgeI site was inserted into exon 1 of Cacna1b by mutagenesis using a QuickChange II XL site-directed mutagenesis kit (Stratagene) with primers 1-For and 1-Rev ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We then ran the digested products on 1% agarose gel and extracted the desired fragments using StrataPrep DNA Gel Extraction Kit (Agilent). We then assembled these two fragments using the NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs ...
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bioRxiv - Microbiology 2021Quote: Mutations were introduced into the HA plasmid of A/Hong Kong/1/1968 using a site-directed mutagenesis kit (QuikChange; Stratagene). 2:6 recombinant IAVs containing wt and modified HA of A/Hong Kong/1/1968 ...
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bioRxiv - Developmental Biology 2021Quote: ... Cy3-labelled probes were prepared from RNA using Low Input Quick Amp Labeling Kit 1-color (Agilent Technologies, #5190-2305) and hybridized with the custom microarrays (ID:085740 ...
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bioRxiv - Genomics 2021Quote: ... The shape and the mean fragment size of the libraries were determined on a 5200 Fragment Analyzer using the HS NGS Fragment Kit 1-6000 bp (both Agilent). Enriched libraries were loaded with a final concentration of 10 pM on a MiSeq Flow Cell using v3 reagent chemistry for 2×150 cycles.
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bioRxiv - Biochemistry 2021Quote: ... Cys to Ala mutants of Flag-tagged DJ-1 were using QuikChange Ⅱ Site-Directed Mutagenesis kit (Agilent Technologies, USA) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... Detection was carried out with the LSAB2 System-HRP (anti-rabbit/mouse LSAB2 Kit Dako Cat#K0679, dilution 1:100) or the VECTASTAIN Elite ABC HRP Kit (Vector Labs ...
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bioRxiv - Microbiology 2024Quote: ... Library size was validated by using Fragment Analyzer 48-Capillary Array with the HS NGS Fragment Kit (1-6000bp) (Agilent). Libraries were sequenced with a Nextseq 2×150 pb paired-end reagent kit (NextSeq 500/550 High Output Kit v2.5 ...
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bioRxiv - Immunology 2024Quote: ... residues D141 of Regnase-1 and D252 of Regnase-3 were mutated to asparigines using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent) following manufacturer’s protocol ...
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bioRxiv - Genetics 2023Quote: ... the Prab-3p::hpk-1 plasmid was used and mutations were performed with a QuikChange II XL Site-Directed Mutagenesis Kit (Agilent). All the assembled plasmids were validated by sequencing prior to microinjection ...
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bioRxiv - Microbiology 2023Quote: ... PCR products are then purified and checked for fragment size distribution on Fragment Analyzer using HS NGS Fragment Kit (1-6000bp) (Agilent).
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bioRxiv - Immunology 2023Quote: ... and Seahorse XF Cell Mito Stress Test Kit (catalog number 103015-100, contains 1 each of oligomycin, FCCP, and rotenone/antimycin A.) were all purchased from Agilent Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... The pcDNA3.1 ERG (GenBank accession. NM_182918) Myc-tagged construct was mutated using the QuickChange Lightning Multi SiteDirected Mutagenesis kit (Agilent: #210513). All primers were designed using the QuickChange Primer Design Program (Agilent ...
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bioRxiv - Cell Biology 2023Quote: ... 1 μM of carbonylcyanide-4-(trifluoromethoxy)-phenylhydrazone (FCCP) (Seahorse XF Cell Energy Phenotype Test Kit from Agilent or Sigma Aldrich), and 1 μM of antimycin A and rotenone (Sigma Aldrich) ...
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bioRxiv - Cancer Biology 2023Quote: ... and finally 0.5 μM rotenone/antimycin A + 1 μg/μL Hoechst (Seahorse XF Cell Mito Stress Test Kit, Agilent, 103015) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The PCR amplicons were subsequently separated by Fragment Analyzer capillary electrophoresis using the DNF905 1-500 base pair kit (Agilent). Bands were quantified and PSI was calculated as follows ...
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bioRxiv - Cell Biology 2024Quote: ... The library profiles were checked using High Sensitivity NGS Fragment Analysis kit (1-6000bp) (Agilent Technologies, reference DNF-474-1000) on the 5300 Fragment Analyzer System ...
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bioRxiv - Cell Biology 2024Quote: ... Qualitative and quantitative measurement of cDNA was conducted with the High Sensitivity NGS Fragment 1-6000bp kit on a 48-channel Fragment Analyzer (Agilent) and 1x dsDNA kit on the Qubit 4 Fluorometer (ThermoFisher) ...
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bioRxiv - Cancer Biology 2024Quote: ... The LZK Q240S drug-resistant construct and K195M kinase-dead mutant (1) was introduced using a Site-Directed Mutagenesis Kit (Stratagene) (Primers listed in Supplementary table S2) ...
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bioRxiv - Cancer Biology 2020Quote: ... KIT (Dako/Agilent), p27Kip1(Fisher/BD Biosciences Pharmingen) ...
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bioRxiv - Cancer Biology 2020Quote: ... KIT (Dako/Agilent), p27Kip1(Fisher/BD Biosciences Pharmingen) ...
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bioRxiv - Cancer Biology 2021Quote: ... KIT (DakoCytomation/Agilent), p27Kip1 (BD Biosciences Pharmingen) ...
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bioRxiv - Cancer Biology 2021Quote: ... KIT (DakoCytomation/Agilent), p27Kip1 (BD Biosciences Pharmingen) ...
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bioRxiv - Neuroscience 2024Quote: ... Envision+ kit (DAKO) was used for chromatic staining ...
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bioRxiv - Neuroscience 2020Quote: ... The quality of the libraries is determined using the Standard High sensitivity NGS Fragment analysis kit (DNF-474, 1-6000 base pair) on the Agilent Fragment analyzer (Agilent, USA), yielding approximately 260 bp size fragments ...
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bioRxiv - Molecular Biology 2022Quote: ... we created pcDNA3-AhR-1(LBD)-VP16 by site-directed mutagenesis of the pcDNA3-AhR-1-VP16 plasmid with the QuikChange II Site-Directed Mutagenesis Kit (Agilent, 200523). The following primer pair was used to create an L to A substitution at L363 ...
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bioRxiv - Neuroscience 2021Quote: ... The quality of the libraries is determined using the Standard High sensitivity NGS Fragment analysis kit (DNF-474, 1-6000 base pair) on the Agilent Fragment analyzer (Agilent, USA), yielding approximately 260 bp size fragments ...
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bioRxiv - Microbiology 2022Quote: ... ΔNef ANCH3 (HIV-1 ANCH3 IND116A) was obtained by insertional mutagenesis using the QuikChange II XL Site-Directed Mutagenesis kit (Agilent #200522), for integrase (IN ...
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bioRxiv - Biochemistry 2022Quote: ... Point mutations (I91F and K176E) were introduced using the QuickChange II XL Site-Directed Mutagenesis Kit (SI Table 1; Agilent Technologies). The full-length sequence of the PAI-1 constructs was confirmed by Sanger sequencing (SI Table 1) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and for fragment size on a Fragment Analyzer using the HS NGS Fragment kit (1–6000 bp) (Agilent Technologies Inc., USA). Final enriched libraries were pooled at equimolar quantities ...
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bioRxiv - Microbiology 2022Quote: ... was performed to remove adapter dimers and 1 μl of the cleaned pool was visualised using the Agilent High Sensitivity DNA Kit for Bioanalyzer 2100 (Agilent Technologies) to confirm adapter-dimer removal.
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bioRxiv - Cell Biology 2023Quote: ... gene expression libraries were constructed using 1-100 ng of each amplified cDNA library and quantified using the TapeStation High Sensitivity D1000 kit (Agilent, Germany) to determine the average fragment size and library concentration ...