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Citations for Agilent :
501 - 550 of 882 citations for CTLA 4 Human HEK293 GST since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... they were stored in 4 mL clear glass vials with polytetrafluoroethylene-lined caps (Agilent, USA), and measurements were performed at 2 ...
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bioRxiv - Plant Biology 2020Quote: ... 4 μg of total RNA with an RNA Integrity Number (RIN) ≥ 8 (Bioanalyzer 2100, Agilent Technologies ...
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bioRxiv - Bioengineering 2021Quote: ... and incubated overnight at 4 °C with primary antibodies: Anti-GFAP 1:1000 (Z0334, Dako), Anti-CD68 1:400 (MCA1957 ...
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bioRxiv - Immunology 2019Quote: ... 4°C overnight followed by HRP conjugated goat-anti-rabbit IgG (#P0448, Dako (1:1000). α-Tubulin was used as a housekeeping control (#sc-32293 ...
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bioRxiv - Plant Biology 2021Quote: ... Hybridizations were done using a custom 4×44 k oligoarray (Agilent Technologies, St. Clara, USA) previously described (Lembke et al. ...
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bioRxiv - Immunology 2021Quote: ... Approximately 4 × 104 ILC2s re-suspended in 50 μL Seahorse XF RPMI pH 7.4 (Agilent) (+ 1M glucose ...
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bioRxiv - Physiology 2022Quote: ... Slides were incubated overnight at 4°C in rabbit anti-GFAP (Agilent Cat# Z0334, RRID:AB_10013382) at a dilution of 1:2000 in antibody diluent (Agilent Cat# S0809) ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated at 4°C overnight with primary antibody in Protein Block (Dako, CA). Sections were rinsed 4x in PBS ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were then washed with PBS and stored at 4°C in Mounting medium (Dako).
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bioRxiv - Molecular Biology 2023Quote: ... on a Qubit 4 fluorometer and a HS NGS Fragment kit (Agilent: DNF-474-1000). Libraries were normalized and pooled to 2nM by calculating the concentration based off the fragment size (base pairs ...
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bioRxiv - Microbiology 2023Quote: ... where non-specific binding cites were blocked with 4% normal rabbit serum (X0902; Dako, DK), the primary antibody was a polyclonal goat anti-IBA1 (ab5076 ...
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bioRxiv - Biochemistry 2023Quote: ... with α-fucosidase (bovine kidney from Sigma-Aldrich or almond α1,3/4-specific from Prozyme), α-mannosidase (jack bean from Sigma) ...
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bioRxiv - Genomics 2019Quote: ... The sheared DNA was used to prepare indexed whole exome sequencing libraries using the Agilent SureSelect XT Human all exon v6 + UTR kit (Agilent Technologies ...
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bioRxiv - Microbiology 2020Quote: ... EnvP(b)1CS- with the furin cleavage site mutated from RKTR to SKTR was generated from the human EnvP(b)1 plasmid using QuikChange site-directed mutagenesis (Agilent). EnvP(b)1 iRBD sequences were synthesized and cloned into a modified pVRC8400 expression vector ...
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bioRxiv - Cell Biology 2019Quote: ... Agilent gene expression microarray 60K slide (Design ID: 72363. SurePrint G3 Human Gene Expression 8 × 60K Microarray Kit, Agilent Technologies) were used ...
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bioRxiv - Cell Biology 2019Quote: ... 2 μg of labeled cRNA was hybridized with a human gene expression microarray 60K (Agilent Technologies, Santa Clara CA, USA). The microarray workflow quality control was implemented using the Agilent Spike-In Kit which consisted of a set of 10 positive control transcripts optimized to anneal to complementary probes on the microarray with minimal self-hybridization or cross-hybridization ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Deletion and site-directed mutants were generated in human ENaC cDNAs cloned into a pGEM HE vector using the QuikChange II Site-Directed Mutagenesis kit (Stratagene) (Ji & Benos ...
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bioRxiv - Immunology 2019Quote: ... Epithelial cells in the EDTA fraction were depleted by incubation with anti-human epithelial antigen antibody (clone Ber-EP4, Dako) followed by anti-mouse IgG dynabeads (ThermoFisher ...
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bioRxiv - Genomics 2019Quote: ... or 100 ng of gDNA was assembled into exome sequencing libraries using the SureSelect Human All Exon V7 Exome kit (Agilent). DNA samples were pooled to achieve an on-bait depth of 100× for the buffy coat gDNA and 300× for the plasma cfDNA.
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bioRxiv - Genomics 2019Quote: ... WES libraries were prepared using the SeqCap EZ Exome Kit v3 (NimbleGen) or the SureSelect Human All Exon V7 Low Input Exome kit (Agilent). Equimolar pooled libraries were sequenced on HiSeq 2000 and 4000 instruments (Illumina ...
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bioRxiv - Genetics 2019Quote: ... into the complete open reading frame of the canonical 307 amino acid human NMNAT2 isoform cloned into expression vector pCMV-Tag2 (Stratagene). The expressed NMNAT2 proteins have a Flag tag and short linker sequence (17 amino acids ...
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bioRxiv - Cancer Biology 2019Quote: ... Library preparation was performed using SureSelect Human All Exon Kit v5 (cat# 5990-9857EN, Agilent Technologies, Santa Clara, CA, USA), sequencing was performed on the Illumina Xten platform ...
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bioRxiv - Systems Biology 2021Quote: ... The relative secreted expression of ARTN was determined by western blotting of culture supernatants using the rabbit antibody HPRK3140989 from the Human Protein Atlas and an HRP-conjugated swine anti-rabbit antibody (p039901-2, Dako) combined with Immobilon Western Chemiluminescent HRP Substrate (Millipore) ...
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bioRxiv - Systems Biology 2021Quote: ... rabbit anti-THBS4 (antibody HPRK2400008 kindly provided from the Human Protein Atlas) as primary antibody and a HRP-conjugated swine anti-rabbit antibody (p039901-2, Dako) combined with TMB substrate (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: Qualitative and quantitative measurements of viral load were determined by quantitative RT-PCR,10 ul of RNA combined with 25 ng Human Universal Reference RNA (Agilent) was purified by PureBeads (Roche Sequencing) ...
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bioRxiv - Cancer Biology 2020Quote: ... 10 – 200 ng of DNA underwent library preparation and exome capture using the SureSelectXT low input protocol with Human All Exon V7 (Agilent) as per manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2020Quote: AAV vectors used in the study were produced in human embryonic kidney HEK 293 cells (AAV-293, Agilent, RRID: CVCL_6871) by an adenovirus-free plasmid transfection method and purified by two rounds of cesium chloride (CsCl ...
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bioRxiv - Cell Biology 2021Quote: ... RNA samples were labeled and hybridized to a SurePrint G3 Human GEO microarray 8 × 60K Ver 3.0 (Agilent, CA, USA), and the raw data were normalized using the 75-percentile shift ...
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bioRxiv - Genomics 2020Quote: ... The BAMfile used for IGV visualization was generated from WES data obtained with the SureSelect Human All Exon V6 kit (exome capture kit) from Agilent. Group alignment is by chromosome of mate ...
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bioRxiv - Cancer Biology 2020Quote: ... Exome regions were captured by an Agilent SureSelect Human All Exon V4 Kit and V5+ LincRNA (Agilent Technologies, CA, USA). Sequencing was performed on an Illumina HiSeq 2000 instrument (Illumina ...
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bioRxiv - Cell Biology 2020Quote: ... Tissues were incubated overnight with primary antibodies (collagen I; rabbit anti-bovine IgG, Cederlane, Burlington, ON, dilution 1:500, collagen IV; mouse anti-human IgG, Dako, Carpinteria ...
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bioRxiv - Cancer Biology 2021Quote: ... Animals treated with CAR T cells had tumors excised and were also stained with H&E and human CD3 (A0452, Dako), in addition to GPC2 ...
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bioRxiv - Cancer Biology 2021Quote: ... Libraries and hybridization capture were performed following the SureSelectXT HS recommended protocol with SureSelect Human All Exon V6+UTR baits (Agilent). Indexed libraries were sequenced on an Illumina NovaSeq with the S4 flow cell (Illumina ...
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bioRxiv - Genomics 2022Quote: ... the three pools were spiked in a diagnostic WES workflow containing 186 exomes (SureSelectXT Low Input Target Enrichment System, Human All Exon V7 probes, Bravo Automated Liquid Handling Platform, Agilent), and 2 whole genome preparations (NEXTFLEX Rapid XP DNA-seq kit ...
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bioRxiv - Neuroscience 2022Quote: ... Human brain sections were rehydrated and antigen demasking was done using EnVisionTM FLEX Target Retrieval Solution (Agilent Technologies, pH 9.0) under heat ...
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bioRxiv - Biochemistry 2022Quote: ... cholerae TrcP was sub-cloned into a custom pET vector and expressed as a 6× His-tagged N-terminal human SUMO2 fusion in E coli BL21 RIL bacteria (Agilent). A 50 ml starter culture grown overnight at 37°C in MDG medium (1.5% Bacto agar ...
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bioRxiv - Neuroscience 2021Quote: Exonic sequences were enriched with the SureSelect Human All Exon 50□Mb V5 Kit (Agilent Technologies, Santa Clara, CA, USA). Sequences were generated on a HiSeq2500 (Illumina ...
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bioRxiv - Cancer Biology 2019Quote: ... Exome capture and sequencing library preparation were performed using Agilent SureSelect Human All Exon V5 (Agilent Genomics, Santa Clara, CA), following standard protocols ...
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bioRxiv - Cancer Biology 2020Quote: ... Kinase dead catalytically inactive versions of Drosophila and human RIOK2 (dRIOK2123A,246A, RIOK2123A,246A) were created using site directed mutagenesis (Lightning Quick Change Kit, Agilent) to make alanine substitutions at catalytic residues Lys-123 and Asp-246 in both human RIOK2 and dRIOK2 as described by other groups (22 ...
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bioRxiv - Immunology 2020Quote: ... Cytospots were stained by May-Gruenwald Giemsa stain and mast cells were detected immunohistochemically (monoclonal mouse anti-human mast cell tryptase, Dako). Staining protocols and further details can be found in the supplementary material ...
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bioRxiv - Biochemistry 2021Quote: ... Mutations of the two Walker-A motifs in human ABCF1 (K324M, K664M) were created by using Quikchange II Site Directed Mutagenesis Kit (Agilent). Human ABCF1 and yeast GCN20 domain fusion cDNAs were created by PCR-mediated ligation and cloned into modified pMtac-His6 vector ...
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bioRxiv - Cancer Biology 2022Quote: ... WES libraries were generated by exome capture of approximately 20,000 coding genes using SureSelect human All exon V6 kit (Agilent Technologies) and paired-end sequencing was performed on a HiSeq sequencer (Illumina ...
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bioRxiv - Pathology 2022Quote: Exonic sequences were enriched in the DNA sample of patient II-6 using SureSelect Human All Exon 50 Mb Kit (Agilent Technologies ...
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bioRxiv - Immunology 2022Quote: ... the ELISA plates were washed 3 times with TBS buffer and a rabbit anti-human HRP antibody 1:3000 (Dako) in blocking solution was applied for 1 hour (RT ...
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bioRxiv - Cancer Biology 2019Quote: ... For cDNA expression microarray analysis using SurePrint G3 Human GE 8×60K Ver.2.0 (Agilent Technologies, Santa Clara, CA, USA), total RNA was sent to a commercial service (Hokkaido System Science ...
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bioRxiv - Immunology 2020Quote: ... and bound mAbs were detected with rabbit anti-human IgG gamma chain-specific/HRP (Agilent: cat. P0214, 1:15,000 dilution).
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bioRxiv - Genetics 2021Quote: ... Partition barcoded libraries were then prepared based on the Chromium Exome Solution (10X Genomics) and the exome target regions was enriched by SureSelect Human All Exon V5 Kit (Agilent) according to the protocols ...
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bioRxiv - Genomics 2019Quote: The FLNC reads from the Isoseq3 refine step were error corrected using Lordec (-k 31 -s 3) with short read RNAseq data from the Universal Human Reference RNA (Agilent) (https://www.ncbi.nlm.nih.gov/sra/SRX1426160 ...
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bioRxiv - Cell Biology 2019Quote: ... total leukocyte DNA was enriched using the Agilent SureSelect Human All Exon 50 Mb capture kit (Agilent Technologies, SantaClara, CA). Bidirectional 100-bp paired-end sequencing (Illumina Hi-Seq 2000 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Quantitative determination of diphenhydramine in human plasma was accomplished by the use of high-performance liquid chromatography with tandem mass spectrometry detection (Agilent Series 1100 LC with MS detector API4000 ...