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Citations for Agilent :
451 - 500 of 830 citations for CTLA 4 Human HEK293 GST since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: Oxford Nanopore MinION long-read sequencing was performed on RNA isolated from an adult human cortex (540005, Agilent Technologies). PolyA-enriched RNA was isolated using the Dynabeads mRNA Direct kit (Life Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... The mutations were introduced into human APP695 using the QuickChange Site-Directed Mutagenesis Kit (Stratagene, San Diego, CA, U.S.) and confirmed by sequencing ...
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bioRxiv - Cancer Biology 2020Quote: ... Exome-sequencing libraries were generated using the Agilent SureSelect Human All Exon V4+UTR capture platform (Agilent, CA, USA). Libraries were sequenced to a target depth of 200-fold coverage on a 100 bp paired-end sequencing run using an Illumina HiSeq 2000 instrument ...
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bioRxiv - Neuroscience 2021Quote: Oxygen Consumption Rate (OCR) from live human iPSC-derived neurons was measured using a Seahorse XFe-96 Analyzer (Agilent). 30,000 human pre-differentiated neurons were seeded per well in Poly-D-Lysine + Laminin-coated 96-well Seahorse plate and differentiated for the indicated time (2 or 4 weeks ...
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bioRxiv - Cancer Biology 2022Quote: The tumor and normal adjacent samples were prepared using SureSelect Human All Exon V7 library (Agilent, Santa Clara USA). 100 bp paired end reads sequencing was performed using the Illumina NovaSeq 6000 system by Edinburgh Genomics (Edinburgh ...
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bioRxiv - Neuroscience 2021Quote: ... Mamu-DR (monoclonal mouse – anti-human HLA-DR/DQ-IgG1, clone CR3/43, cat. no. M077501-2, Agilent Technologies), 1:150 ...
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bioRxiv - Genetics 2020Quote: ... enrichment and elution were performed according to manufacturer’s instructions and protocols (SureSelect Human All Exon Kits Version CRE, Agilent). The library was sequenced on an Illumina HiSEQ 2500 as paired-end 75bp reads ...
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bioRxiv - Cancer Biology 2022Quote: ... tumor mutational burdens were computed using the exonic ranges from the SureSelect Human All Exon v7 panel from Agilent (bed file downloaded from the manufacturer’s website ...
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bioRxiv - Cancer Biology 2022Quote: ... The following antibodies were used: AMACR/p63 immunohistochemistry (IHC) double staining (Monoclonal Rabbit Anti-Human AMACR, clone 13H4, Dako, Code M361601-2 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... SCA-associated mutations were introduced into human cMyc-mGlu1 using Quikchange site-directed mutagenesis (Agilent Technologies, Santa Clara, CA). Mutagenesis primers are shown in Table S1 ...
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bioRxiv - Cell Biology 2023Quote: Single point mutations were inserted in the human RAB6A sequence at position 156 using the QuickChange mutagenesis kit (Agilent).
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bioRxiv - Biophysics 2023Quote: Human wild-type α-synuclein and A90C cysteine variants were purified from Escherichia coli BL21 (DE3)-gold (Agilent Technologies) expressing plasmid pT7-7 encoding for α-synuclein as previously described35,42,43 ...
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bioRxiv - Immunology 2024Quote: ... Microarray analysis was performed at TaKaRa-Bio using high-density oligonucleotide array (Human Genome U133 Plus 2.0) and GeneSpring 12.5 software (Agilent Technologies) as described previously (17) ...
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bioRxiv - Cell Biology 2019Quote: ... for 1hr and incubated OVN at 4°C with indicated primary antibodies: αSMA (Dako, M0851); Desmin (Abcam ...
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bioRxiv - Molecular Biology 2020Quote: ... and crosslinking was performed at 100mJ/cm2 at 4°C in a Stratalinker 1800 (Stratagene). Crosslinked cells where harvested by scraping in cold 1xPBS and pelleted at 700xg for 2min ...
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bioRxiv - Cancer Biology 2020Quote: ... Each section was treated with 4 drops of serum-free protein block (Agilent cat # X0909) for 20 minutes at room temperature ...
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bioRxiv - Genetics 2019Quote: ... anserina consisted of a custom 4×44 K platform (AMADID 018343, Agilent, Santa Clara, USA) containing 10,556 probes on each array with each probe in four replicates ...
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bioRxiv - Genetics 2022Quote: ... the DNAs were hybridized on CGH 4 × 180 K mouse slides (AMADID 027411, Agilent Technologies). Finally ...
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bioRxiv - Developmental Biology 2019Quote: ... Dye-swap hybridizations (2+2) were performed on microarray slides (4×44K zebrafish V3, Agilent) using gasket slides and a hybridization chamber and incubated for 17 h at 65°C and 10 rpm in the hybridization oven (Agilent Technologies) ...
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bioRxiv - Pathology 2020Quote: ... Primary antibodies were incubated overnight at 4°C and appropriate HRP-conjugated secondary antibodies (DAKO, Agilent Technologies ...
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bioRxiv - Microbiology 2021Quote: ... Total RNA from 4 independent replicates were checked on RNA6000 RNA chips on Bioanalyzer (Agilent) for its quality and integrity ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... they were stored in 4 mL clear glass vials with polytetrafluoroethylene-lined caps (Agilent, USA), and measurements were performed at 2 ...
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bioRxiv - Plant Biology 2020Quote: ... 4 μg of total RNA with an RNA Integrity Number (RIN) ≥ 8 (Bioanalyzer 2100, Agilent Technologies ...
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bioRxiv - Bioengineering 2021Quote: ... and incubated overnight at 4 °C with primary antibodies: Anti-GFAP 1:1000 (Z0334, Dako), Anti-CD68 1:400 (MCA1957 ...
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bioRxiv - Immunology 2019Quote: ... 4°C overnight followed by HRP conjugated goat-anti-rabbit IgG (#P0448, Dako (1:1000). α-Tubulin was used as a housekeeping control (#sc-32293 ...
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bioRxiv - Plant Biology 2021Quote: ... Hybridizations were done using a custom 4×44 k oligoarray (Agilent Technologies, St. Clara, USA) previously described (Lembke et al. ...
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bioRxiv - Immunology 2021Quote: ... Approximately 4 × 104 ILC2s re-suspended in 50 μL Seahorse XF RPMI pH 7.4 (Agilent) (+ 1M glucose ...
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bioRxiv - Physiology 2022Quote: ... Slides were incubated overnight at 4°C in rabbit anti-GFAP (Agilent Cat# Z0334, RRID:AB_10013382) at a dilution of 1:2000 in antibody diluent (Agilent Cat# S0809) ...
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bioRxiv - Molecular Biology 2023Quote: ... on a Qubit 4 fluorometer and a HS NGS Fragment kit (Agilent: DNF-474-1000). Libraries were normalized and pooled to 2nM by calculating the concentration based off the fragment size (base pairs ...
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bioRxiv - Microbiology 2023Quote: ... where non-specific binding cites were blocked with 4% normal rabbit serum (X0902; Dako, DK), the primary antibody was a polyclonal goat anti-IBA1 (ab5076 ...
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bioRxiv - Biochemistry 2023Quote: ... with α-fucosidase (bovine kidney from Sigma-Aldrich or almond α1,3/4-specific from Prozyme), α-mannosidase (jack bean from Sigma) ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were then washed with PBS and stored at 4°C in Mounting medium (Dako).
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated at 4°C overnight with primary antibody in Protein Block (Dako, CA). Sections were rinsed 4x in PBS ...
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bioRxiv - Genomics 2019Quote: ... The sheared DNA was used to prepare indexed whole exome sequencing libraries using the Agilent SureSelect XT Human all exon v6 + UTR kit (Agilent Technologies ...
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bioRxiv - Microbiology 2020Quote: ... EnvP(b)1CS- with the furin cleavage site mutated from RKTR to SKTR was generated from the human EnvP(b)1 plasmid using QuikChange site-directed mutagenesis (Agilent). EnvP(b)1 iRBD sequences were synthesized and cloned into a modified pVRC8400 expression vector ...
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bioRxiv - Cell Biology 2019Quote: ... Agilent gene expression microarray 60K slide (Design ID: 72363. SurePrint G3 Human Gene Expression 8 × 60K Microarray Kit, Agilent Technologies) were used ...
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bioRxiv - Cell Biology 2019Quote: ... 2 μg of labeled cRNA was hybridized with a human gene expression microarray 60K (Agilent Technologies, Santa Clara CA, USA). The microarray workflow quality control was implemented using the Agilent Spike-In Kit which consisted of a set of 10 positive control transcripts optimized to anneal to complementary probes on the microarray with minimal self-hybridization or cross-hybridization ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Deletion and site-directed mutants were generated in human ENaC cDNAs cloned into a pGEM HE vector using the QuikChange II Site-Directed Mutagenesis kit (Stratagene) (Ji & Benos ...
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bioRxiv - Immunology 2019Quote: ... Epithelial cells in the EDTA fraction were depleted by incubation with anti-human epithelial antigen antibody (clone Ber-EP4, Dako) followed by anti-mouse IgG dynabeads (ThermoFisher ...
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bioRxiv - Genomics 2019Quote: ... or 100 ng of gDNA was assembled into exome sequencing libraries using the SureSelect Human All Exon V7 Exome kit (Agilent). DNA samples were pooled to achieve an on-bait depth of 100× for the buffy coat gDNA and 300× for the plasma cfDNA.
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bioRxiv - Genomics 2019Quote: ... WES libraries were prepared using the SeqCap EZ Exome Kit v3 (NimbleGen) or the SureSelect Human All Exon V7 Low Input Exome kit (Agilent). Equimolar pooled libraries were sequenced on HiSeq 2000 and 4000 instruments (Illumina ...
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bioRxiv - Genetics 2019Quote: ... into the complete open reading frame of the canonical 307 amino acid human NMNAT2 isoform cloned into expression vector pCMV-Tag2 (Stratagene). The expressed NMNAT2 proteins have a Flag tag and short linker sequence (17 amino acids ...
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bioRxiv - Cancer Biology 2019Quote: ... Library preparation was performed using SureSelect Human All Exon Kit v5 (cat# 5990-9857EN, Agilent Technologies, Santa Clara, CA, USA), sequencing was performed on the Illumina Xten platform ...
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bioRxiv - Systems Biology 2021Quote: ... The relative secreted expression of ARTN was determined by western blotting of culture supernatants using the rabbit antibody HPRK3140989 from the Human Protein Atlas and an HRP-conjugated swine anti-rabbit antibody (p039901-2, Dako) combined with Immobilon Western Chemiluminescent HRP Substrate (Millipore) ...
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bioRxiv - Systems Biology 2021Quote: ... rabbit anti-THBS4 (antibody HPRK2400008 kindly provided from the Human Protein Atlas) as primary antibody and a HRP-conjugated swine anti-rabbit antibody (p039901-2, Dako) combined with TMB substrate (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: Qualitative and quantitative measurements of viral load were determined by quantitative RT-PCR,10 ul of RNA combined with 25 ng Human Universal Reference RNA (Agilent) was purified by PureBeads (Roche Sequencing) ...
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bioRxiv - Cancer Biology 2020Quote: ... 10 – 200 ng of DNA underwent library preparation and exome capture using the SureSelectXT low input protocol with Human All Exon V7 (Agilent) as per manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2020Quote: AAV vectors used in the study were produced in human embryonic kidney HEK 293 cells (AAV-293, Agilent, RRID: CVCL_6871) by an adenovirus-free plasmid transfection method and purified by two rounds of cesium chloride (CsCl ...
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bioRxiv - Cell Biology 2021Quote: ... RNA samples were labeled and hybridized to a SurePrint G3 Human GEO microarray 8 × 60K Ver 3.0 (Agilent, CA, USA), and the raw data were normalized using the 75-percentile shift ...
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bioRxiv - Genomics 2020Quote: ... The BAMfile used for IGV visualization was generated from WES data obtained with the SureSelect Human All Exon V6 kit (exome capture kit) from Agilent. Group alignment is by chromosome of mate ...