Labshake search
Citations for Agilent :
5301 - 5350 of 5930 citations for Rat N acylethanolamine hydrolyzing acid amidase NAAA ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... All the fusion constructs were made from the appropriate backbone vector with the QuikChange Lightning Multi Site Directed Mutagenesis kit (Agilent Technologies). Reporters were sequenced from Genewiz before transfecting the parasites ...
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bioRxiv - Microbiology 2023Quote: ... AIP56D274S/D276-278S and AIP56D274N/D276-278N with or without V5-tag were generated with the QuickChange Site-Directed Mutagenesis Kit (Stratagene, 200518) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... and library profiles were assessed using a High Sensitivity DNA kit on an Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). Libraries were then sequenced on an Illumina NovaSeq instrument (Illumina ...
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bioRxiv - Plant Biology 2023Quote: ... The final library was assessed for quality using the Agilent TapeStation System with a D1000 kit (Agilent Technologies, Santa Clara, California), and paired-end ...
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bioRxiv - Cell Biology 2023Quote: The NR2A(M705V) or NR2A(A727T) mutation was introduced to the NR2A subunit using QuikChange II site-directed mutagenesis Kit (Agilent Genomics). DNA sequencing was used to confirm the cDNA sequences ...
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bioRxiv - Cancer Biology 2023Quote: ... The quality and quantity of libraries were determined using Fragment Analyzer by DNF-474 High Sensitivity NGS Fragment Analysis Kit (Agilent Technologies) and QuantiFluor dsDNA System (Promega) ...
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bioRxiv - Cancer Biology 2023Quote: Deletions of SP1 sites on human MGMT promoter were performed using Quick-Change XL Site-Directed Mutagenesis kit (Agilent Technologies, USA).34 The mutated promoters were further cloned into pGL3 basic vector ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Fragment size distributions of tagmented and dual indexed libraries were analyzed by capillary gel electrophoresis using a Bioanalyzer High Sensitivity DNA kit (50067-4626, Agilent, USA). Up to 384 high quality libraries with size distribution between 250-1000bp were pooled using equal molar quantities ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... as previously described.19 Site-directed mutagenesis was performed using the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent, Santa Clara, CA) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... the integrity of DNAse I-treated RNA was determined using the Agilent RNA 6000 Pico Kit and an Agilent 2100 Bioanalyzer (Agilent Technologies). RNA with RIN values > 4 was used for RT.
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bioRxiv - Genomics 2023Quote: ... The concentration and quality of the sample was evaluated with Qubit dsDNA HS kit and Tapestation D5000 Screen Tape (Agilent Technologies). The expected recovery was above 50 ng.
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bioRxiv - Cell Biology 2023Quote: The quantity and quality of RNA samples was assessed using the standard sensitivity RNA fragment analysis kit on Fragment Analyzer (Agilent Technologies). All samples had an RNA integrity number >8 and were deemed of sufficient quantity and quality for RNA-seq analysis ...
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bioRxiv - Cell Biology 2023Quote: ... Integrity and concentrations of the RNA samples were determined using an Agilent 2100 bioanalyzer with RNA 6000 Nano Kit (Agilent, USA). Libraries for 6 samples were prepared using the Ion Total RNA-Seq Kit v2 according to the manufacturer’s protocol using 12-22 polyA-enriched RNA as input material ...
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bioRxiv - Cell Biology 2023Quote: ... TP53-missense mutations and protospacer adjacent motif (PAM) sequence mutation were introduced using the QuikChange Lightning site-directed mutagenesis kit (Agilent Technologies). A puromycin or neomycin resistance cassette was cloned into the BamHI site which was placed between left- and right-arm of the amplified and cloned DNA fragments ...
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bioRxiv - Biochemistry 2023Quote: ... was used as a template to generate deletion variants for expression in insect cells using the QuickChange II XL site-directed mutagenesis kit (Agilent Technologies). For bacterial expression of the ΔN790 variant ...
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bioRxiv - Plant Biology 2023Quote: ... and the quality assessed with an Agilent 2100 Bioanalyzer with an RNA 144000 Nano Kit (Agilent technologies, Palo Alto, CA, USA).
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bioRxiv - Microbiology 2023Quote: Site-directed mutagenesis of SARS-CoV-2 spike was performed with the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent 200522), using primers listed in Supplementary table S2 and according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The above residue mutants were created by site-directed mutagenesis following instructions provided in the QuickChange XL Site-Directed Mutagenesis Kit manual (Agilent Technologies). All other full-length UNK residue mutants ...
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bioRxiv - Microbiology 2023Quote: ... The quality and quantity of the amplicons were checked using the D1000 ScreenTape Assay kit read on a 2100 TapeStation system (Agilent Technologies) and a Qubit dsDNA BS assay kit (Invitrogen) ...
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bioRxiv - Microbiology 2023Quote: ... into AAATA in the DENV-2 wild type plasmid (pFK-DVs) was generated using QuickChange XL site-directed mutagenesis kit (Agilent, 200517) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... fluorescent-based assays were used including qPCR with the Universal KAPA Library Quantification Kit and Fragment Analyzer (Advanced Analytics) or BioAnalyzer (Agilent 2100). Then ...
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bioRxiv - Molecular Biology 2023Quote: ... Quality control for the constructed library was performed by the Agilent Bioanalyzer High Sensitivity DNA Kit (Agilent Technologies; Catalog # 5067-4626), while Qubit DNA HS Assay Kit was used for qualitative and quantitative analysis ...
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bioRxiv - Neuroscience 2023Quote: ... total RNA was quantified and quality checked using Bioanalyzer 2100 instrument in combination with RNA 6000 nano kit (both Agilent Technologies). Libraries were prepared from 800 ng of input material (total RNA ...
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bioRxiv - Molecular Biology 2023Quote: ... The desired PNUTS mutant in the RVXF motif (converting the RISW motif to RASA) was generated by oligonucleotide-mediated site-directed mutagenesis (QuikChange II XL Site-Directed Mutagenesis Kit, Agilent Technologies) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... RNA quality and quantity were checked using RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). Library construction ...
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bioRxiv - Immunology 2023Quote: ... and quality was verified on an Agilent 2100 Bioanalyzer instrument with the Agilent DNA 1000 kit (Agilent Technologies, Catalog# 5067-1504). Additionally ...
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bioRxiv - Genetics 2023Quote: ... RNA quantity and quality were assessed using NanoDrop ND-1000 Spectrophotometer (Nanodrop Technologies) and Agilent 2100 Bioanalyser (RNA 6000 Nano LabChip kit, Agilent Technologies).
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LSD1 inhibition improves efficacy of adoptive T cell therapy by enhancing CD8+ T cell responsivenessbioRxiv - Immunology 2023Quote: Seahorse experiments were performed on sorted CD8+ cells activated in presence or absence of LSDi using XF Cell Mito Stress kit (Seahorse Bioscience). OCR and ECAR were measured with XF96 Extracellular Flux Analyzers (Seahorse Bioscience) ...
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bioRxiv - Immunology 2023Quote: ... Endogenous peroxidase was blocked using Peroxidase block (Refine Kit) followed by Protein block (X090930-2, Agilent Technologies Inc., Santa Clara, CA). Primary antibodies were applied at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... Two μl of RNA samples was used for reverse transcription with the AccuScript High-Fidelity 1st Strand cDNA Synthesis Kit (Agilent; 200820) using random hexamer primers ...
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bioRxiv - Immunology 2023Quote: ... The size of the resulting libraries was determined with the Agilent D1000 reagent kit and Agilent 4200 TapeStation (Agilent Technologies, USA). Pooling was performed automatically using a Tecan Freedom EVO robotic station (Tecan ...
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bioRxiv - Microbiology 2023Quote: ... The purified libraries were then assessed for their fragment sizes using the High Sensitivity DNA kit on the Bioanalyzer 2100 (Agilent Technologies). After applying the NetoVIR protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The quality of each library was assessed using a Bioanalyzer high sensitivity kit on an Agilent 2100 Bioanalyzer (Agilent 5067-4626). The libraries were submitted for sequencing at Cancer Research UK Cambridge Institute Genomic Core Facility using Illumina NovaSeq with 100bp single-end reads.
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bioRxiv - Cancer Biology 2023Quote: ... Single-cell RNA-seq and CMO libraries were prepared according to the manufacturer’s protocol and the library quality was confirmed with a Bioanalyzer High-Sensitivity DNA Kit (Agilent, 5067-4627). Cellecta barcode libraries were prepared using 25% of the adaptor-ligated cDNA to amplify only the barcoded cDNA product ...
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bioRxiv - Cell Biology 2023Quote: ... Quantification and quality checked of libraries was done using an Agilent 2100 Bioanalyzer instrument and a DNA 7500 kit (Agilent Technologies). Libraries were pooled and sequenced on a NovaSeq6000 S1 100 cycle run (v1.5 SBS reagents) ...
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bioRxiv - Cell Biology 2023Quote: The NGS sample was diluted with TE buffer and assayed using a High Sensitivity DNA Kit (cat. #5067-4626, Agilent Technologies) on a Bioanalyzer 2100 ...
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bioRxiv - Biochemistry 2023Quote: Mutations were generated by site-directed mutagenesis (table 2) on this plasmid using QuikChange II Site-Directed Mutagenesis Kit (Agilent Technologies) and plasmids were verified through sequencing through the Molecular Informatics Core at the University of Rhode Island.
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bioRxiv - Biochemistry 2023Quote: ... The desired PP1 or PNUTS mutants were generated by oligonucleotide- mediated site-directed mutagenesis (QuikChange II XL Site-Directed Mutagenesis Kit, Agilent Technologies) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Site-specific mutagenesis was performed according to standard procedures of the QuickChange Site-Directed Mutagenesis Kit purchased from Stratagene (GE Healthcare) or Mut Express II (Vazyme Biotech) ...
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bioRxiv - Biophysics 2023Quote: ... H80S/D83S and G93A mutants were generated by the use of QuikChange Site-Directed Mutagenesis Kit (Stratagene, La Jolla, CA, USA) (60) ...
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bioRxiv - Plant Biology 2023Quote: ... Protease activity of NIa-Pro was abolished by mutating cysteine 151 to alanine (C151A) using the same construct and the QuikChange Lightning Site-Directed Mutagenesis kit (Agilent, USA) in both pMDC32 and pSITE (Huogen et al. ...
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bioRxiv - Genomics 2023Quote: ... and RNA integrity was checked with an RNA Kit on an Agilent 5300 Fragment Analyzer (Agilent Technologies, Palo Alto, CA, USA). RNA sequencing libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina following the manufacturer’s instructions (NEB ...
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bioRxiv - Pathology 2024Quote: ... and the integrity of the RNAs was verified using the Agilent 2100 bioanalyzer with the RNA 6000 Nano kit (Agilent Technologies). 1 µg of total RNA was reverse transcribed in a 20 µL μnal reaction volume using the High-Capacity Reverse Transcription kit (Applied Biosystems ...
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bioRxiv - Cell Biology 2024Quote: ... The INF2-ER[K792A] GFP construct was generated via site-directed mutagenesis using the QuikChange II Site-Directed Mutagenesis Kit (Agilent Technologies). All constructs were sequenced completely across their coding region.
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bioRxiv - Genetics 2024Quote: ... was used for all experiments with integrated Cas9 (Supplementary Table 3).25 Cas9D10A was generated by site-directed mutagenesis of pLS504 using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent #200521) using primers MK11 and MK12 ...
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bioRxiv - Cell Biology 2024Quote: ... The S71A and S71E mutations were introduced into the pDONR221-gCDC42attb plasmid with the QuickChange II XL Site Directed Mutagenesis Kit (#200521, Agilent Technologies) with the following primers ...
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bioRxiv - Cell Biology 2024Quote: ... The integrity and quantity of the total RNA was measured using an Agilent 2100 Bioanalyzer RNA 6000 Nano Kit (Agilent Technologies). Total RNA obtained from each sample was subjected to sequencing library construction using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs ...
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bioRxiv - Cell Biology 2024Quote: ... The S71A mutation was introduced into the pMALc-MBP::CDC-42 plasmid with the QuickChange II XL Site directed mutagenesis kit (#200521, Agilent technologies) with the following primers ...
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bioRxiv - Cell Biology 2024Quote: The aforementioned mutations in the hypervariable regions of RhoA-GFP and RhoB-GFP were generated according to the manufacturing instructions of the QuickChange Lightning Site-Directed Mutagenesis kit (Agilent Technologies) using the mutagenic primers listed in the Key Resources Table ...
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bioRxiv - Cell Biology 2024Quote: Cy3-labeled cRNAs were further hybridized onto Agilent Whole Human Genome kit 4 x 44K slides (Catalog number G4112F, Agilent Technologies) containing 44,397 oligonucleotide probes in 2X Hi-RPM Hybridization buffer (Catalog number 5188-5242 ...