Labshake search
Citations for Agilent :
5401 - 5450 of 5601 citations for Rat CD325 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... The fragmented RNA was analyzed on an Agilent 2100 bioanalyzer with an Agilent RNA 6000 Pico kit (Agilent Technologies, Santa Clara, CA, USA) to determine the length distribution of the fragments ...
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bioRxiv - Cancer Biology 2023Quote: ... The exome region of the whole genome libraries was enriched with the Agilent SureSelectXT Human All Exon Kit V6 (Agilent; Santa Clara, CA) and sequenced on the NovaSeq 6000 Sequencing System (Illumina ...
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bioRxiv - Genomics 2022Quote: ... we assessed the quality of the RNA by quantifying the RNA integrity number (RIN) for each sample using the Agilent RNA 6000 Pico kit (Agilent, Santa Clara, CA) on the Agilent 2100 Bioanalyzer (Agilent ...
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bioRxiv - Cell Biology 2023Quote: ... The human GABAA receptor α1 subunit missense mutations (S76R, R214C, D219N, G251D, P260L, M263T, T289P, and A322D) were constructed using QuikChange II site-directed mutagenesis Kit (Agilent Genomics, catalog #: 200523). Enhanced cyan fluorescent protein (CFP ...
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bioRxiv - Molecular Biology 2023Quote: ... The purified DNA library was analyzed using an Agilent 2100 bioanalyzer with an Agilent DNA 1000 Kit (Agilent Technologies, Santa Clara, CA, USA) and a Qubit 2.0 with Qubit 1X dsDNA HS Assay Kits (Thermo Fisher Scientific ...
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Epigenetic deregulation of IFN and WNT pathways in AT2 cells impairs alveolar regeneration (in COPD)bioRxiv - Cell Biology 2023Quote: ... RNA concentration and integrity were measured using the RNA Pico 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA), and only samples with RIN > 8 were included in RNA-seq ...
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bioRxiv - Physiology 2023Quote: ... and the quality (e.g. fragment length) was assessed for a subset using a Bioanalyzer High Sensitivity DNA chip kit (Agilent Technologies, Santa Clara, CA). Single-end sequencing with 100-bp read length was conducted on a NovaSeq platform (Illumina ...
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bioRxiv - Molecular Biology 2023Quote: ... Quality of the RNA samples (RIN score >7) was verified on the Agilent 2100 Bioanalyzer using the RNA 6000 kit (Agilent, cat# 5067-1513). 1 µg of each RNA sample was then used for poly(A ...
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bioRxiv - Neuroscience 2023Quote: ... Beads-based double size selection was performed to ensure the fragment size of each sample was between 300-600 bp as measured by an Agilent DNA High Sensitivity NGS Fragment Analysis Kit (Agilent, DNF-474-0500). The concentration of ligated fragments in each library was quantified with the KAPA Library Quantification Kits for Illumina platforms (Roche/KAPA Biosystems ...
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bioRxiv - Molecular Biology 2023Quote: ... quantitation and sample purity were assessed using the RNA Nano 6000 Assay Kit of the Agilent Bioanalyzer 2100 system (Agilent Technologies, CA, USA) and agarose gel electrophoresis ...
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bioRxiv - Cancer Biology 2023Quote: ... The oxygen consumption rate (OCR) assay was performed using the XF long-chain fatty acid oxidation (LCFA) stress test kit (Agilent, cat# 103672-100) and Seahorse XFe24 Analyzer was used to investigate the long-chain fatty acid OCR according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2023Quote: ... and leucine (Leu41) within the conserved destruction box were mutagenized to alanine (RxxL-> AxxA) using the Quickchange mutagenesis kit (Stratagene, La Jolla, CA). Worms were transformed with the pJK3 plasmid carrying gfp::ify-1dm using microparticle bombardment as previously described (Praitis et al. ...
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bioRxiv - Cell Biology 2023Quote: ... Total amounts and integrity of RNA were assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). Total RNA was used as input material for RNA sample preparations ...
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bioRxiv - Developmental Biology 2023Quote: ... We performed exome sequencing in the child with diabetes and his consanguineous parents and sibling using the Agilent SureSelect Human All Exon Kit (Agilent SureSelect v4, 50Mb) and next-generation sequencing (Hiseq ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA concentration and quality was measured using the RNA 6000 Pico kit on the Bioanalyzer 2100 capillary electrophoresis system (Agilent, Santa Clara, CA) and stored at −80°C ...
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bioRxiv - Genetics 2024Quote: ... poly(A)+ RNA was separated and purified from total RNA using an oligo(dT) cellulose column Poly(A) Quick mRNA Isolation Kit (Stratagene, La Jolla, CA). Single-stranded cDNAs were synthesized by reverse transcription reaction using 0.25 U AMV reverse transcriptase (Life Sciences Advanced Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... while the concentration and integrity of RNA samples were detected by using Agilent 2100 RNA nano 6000 assay kit (Agilent Technologies, CA, USA). Subsequently ...
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bioRxiv - Biophysics 2024Quote: Introduction of the 118C mutation into the T4L gene (pET11a vector) was accomplished using the QuikChange site-directed mutagenesis kit (Agilent; Santa Clara, CA) and confirmed by DNA sequencing ...
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bioRxiv - Cell Biology 2024Quote: ... Mutations were constructed by site-directed mutagenesis according to the instructions accompanying the Quik Change ® Site-Directed Mutagenesis Kit (Agilent Technologies, Stratagene). Conventional PCRs reactions were performed with KAPA Taq DNA polymerase (Kapa Biosystems ...
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bioRxiv - Cancer Biology 2024Quote: The oxygen consumption rate (OCR) of cells was measured using the Seahorse Cell Mito Stress Test kit following the user guide (Agilent, cat. 103010-100). Briefly ...
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bioRxiv - Biochemistry 2024Quote: ... Average fragment lengths of 40-50 kbp were confirmed using a Fragment Analyzer (Advanced Analytical) with an HS Large Fragment 50 Kb analysis kit (Agilent, DNF-464-33). Samples were prepared for Oxford Nanopore Technologies (ONT ...
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bioRxiv - Microbiology 2024Quote: ... To determine the RNA integrity number the isolated RNA was run on an Agilent Bioanalyzer 2100 using an Agilent RNA 6000 Nano Kit as recommended by the manufacturer (Agilent Technologies, Waldbronn, Germany). Remaining genomic DNA was removed by digesting with TURBO DNase (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... Library qualities were assessed using the Agilent High Sensitive DNA Kit (#5067-4626) on an Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, USA). Qualified libraries underwent paired-end sequencing on an Illumina NovaSeq System (Illumina ...
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bioRxiv - Cell Biology 2024Quote: ... a DeNovix DS-11 FX spectrophotometer and an Agilent FEMTO Pulse System with a Genomic DNA 165 kb Kit (Agilent, FP-1002-0275), respectively ...
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bioRxiv - Cell Biology 2024Quote: ... and RNA integrity was assessed using the Agilent TapeStation 4200 and the Agilent RNA ScreenTape Assay Kit (Agilent; 5067-5576 and 5067-5577).
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bioRxiv - Plant Biology 2024Quote: ... The quality of the barcoded cDNA libraries was assessed with the High Sensitivity DNA Analysis Kit in the Agilent 2100 Bioanalyzer (Agilent Technologies, United States) and then sequenced (150 bp paired-end reads ...
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bioRxiv - Genetics 2024Quote: ... RNA concentration and quality was assessed using an Agilent 2100 Bioanalyzer with the RNA Nano 6000 kit (Agilent Technologies, Santa Clara, CA, USA), and only RNA samples with RIN >7 were processed for subsequent cDNA library construction and sequencing ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA integrity (RIN) was determined using the RNA Nano 6000 Assay Kit with the Agilent Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA, USA). RIN values ranged from 8.2-10 for all samples ...
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bioRxiv - Genetics 2021Quote: ... RNA integrity (RIN) was determined using the RNA Nano 6000 Assay Kit with the Agilent Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA, USA). RIN values ranged from 7.3–10 for all samples ...
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bioRxiv - Developmental Biology 2020Quote: ... analysis was performed using TransStart Tip Green qPCR SuperMix kit (TransGen Co.) on a Stratagene Mx3000P real-time PCR system (Stratagene, La Jolla, CA, USA) under the conditions of 94 °C for 30 s ...
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bioRxiv - Genomics 2019Quote: ... and successful size-selection verified by electrophoresis on an Agilent TapeStation using the Genomic DNA High ScreenTape kit (Agilent Technologies, Santa Clara, CA, USA).
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Standard quality control steps were included to determine total RNA quantity and quality using Agilent 2100 Bioanalyzer with Eukaryote Total RNA Pico Kit and High Sensitivity DNA Assay (Agilent Technologies, Santa Clara, CA). Libraries were prepared using the TruSeq Stranded mRNA Library preparation Kit (Illumina Inc. ...
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bioRxiv - Cancer Biology 2020Quote: ... early and late nascent strands were labeled with Cy3 and Cy5 ULS molecules using the Genomic DNA labeling Kit (Agilent, Santa Clara, CA, USA) following manufacturer's instructions ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Plasmid DNA mutations in the predicted orthosteric binding site of PAR4 were generated using the QuikChange XL Multi Site-Directed Mutagenesis Kit (Agilent Technologies, Mississauga, ON, Canada) to generate all mutants described in this study ...
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bioRxiv - Physiology 2019Quote: ... RNA quality and integrity were validated using a NanoDrop ND-1000 UV–vis Spectrophotometer (NanoDrop Technologies, Wilmington, USA) and Agilent 2100 Bioanalyzer and RNA 6000 Nano LabChip kit (Agilent Technologies, Palo Alto, USA) respectively ...
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bioRxiv - Immunology 2019Quote: Various N-linked glycosylation site mutants of UL144 were generated by site-directed mutagenesis using Quick Change II site directed mutagenesis Kit (Stratagene, La Jolla, CA, USA). The Asn residues carrying the N-linked glycan were mutated individually or in combination at residues N61 ...
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bioRxiv - Neuroscience 2019Quote: ... The resulting supernatants were transferred to new tubes and 50 µl of each cleared lysate was mixed with 50 µl Lysis Buffer (0.7 µl β-mercaptoethanol/100 µl Lysis Buffer; Agilent Absolutely RNA Nanoprep Kit) and stored at –80° for later preparation as input RNA ...
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bioRxiv - Genomics 2021Quote: ... Libraries from all sample sets were quantified on the 2100 Bioanalyzer instrument with the Agilent DNA 7500 Kit (both Agilent Technologies, Santa Clara, CA).
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bioRxiv - Bioengineering 2022Quote: ... Size pattern was assessed with Fragment Analyzer-96 with the DNF-474-0500 High Sensitivity NGS Fragment Analysis Kit (Agilent Technologies, DNF-474-0500). Libraries (Average size ...
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bioRxiv - Biochemistry 2021Quote: Mutated forms of rat and human BDH2 enzymes (Y157F and Y147F, respectively) were generated by site-directed mutagenesis using a QuikChange II XL kit (Stratagene, La Jolla, CA, USA), with pCOLD I/rBDH2 and pCOLD I/hBDH2 plasmids as templates and mutagenic primers Y157F-rBDH2-S and Y157F-rBDH2-AS as well as Y147F-hBDH2-S and Y147F-hBDH2-AS ...
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bioRxiv - Neuroscience 2020Quote: ... the purity and concentration of RNA samples were determined by readings of OD260/280 using a dual beam UV spectrophotometer and RNA integrity was determined by capillary electrophoresis using the RNA 6000 Nano Lab-on-a-Chip kit and the Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA) following the manufacturers instructions ...
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bioRxiv - Biochemistry 2020Quote: ... and Ile-residues of the HXXXDX motif of ZmGL2 and ZmGL2-LIKE were generated using QuikChange XL Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA). The ZmGL2 protein was also expressed in E ...
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bioRxiv - Systems Biology 2020Quote: ... The sub-libraries were each resuspended to a final volume of 20 μL in nuclease-free water and fragment distribution was checked running a bioanalyzer (Agilent High Sensitivity DNA Kit) according to the manufacturer’s protocol.
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bioRxiv - Plant Biology 2021Quote: ... The fragment sizes and concentrations of the pooled samples were determined on a Fragment analyzer 5200 using the DNF-473-Standard Sensitivity NGS Fragment Analysis Kit (Agilent, Santa Clara, CA, USA). The indexing PCR was performed under the following conditions ...
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bioRxiv - Neuroscience 2020Quote: ... The resulting supernatants were transferred to new tubes and 50 μl of each cleared lysate was mixed with 50 μl Lysis Buffer (0.7 μl β-mercaptoethanol/100 μl Lysis Buffer; Agilent Absolutely RNA Nanoprep Kit) and stored at −80°C for later preparation as Input RNA ...
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bioRxiv - Microbiology 2020Quote: ... Libraries were pooled in equal quantity and combined to multiplex to make a final library and QC was done again using Qubit quantification kit and Agilent bioanalyzer using high sensitivity DNA chip (Agilent Biotechnology, catalog # 5067-4626). The final combined library was sequenced using Illumina technology on NextSeq 500 sequencer using High output 300-bp Single end Read sequencing kit.
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bioRxiv - Plant Biology 2021Quote: ... Other related alleles required for transient expression or infection analyses were either created using the QuickChange Site-directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA) or synthesised by Twist Bioscience (South San Francisco ...
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bioRxiv - Neuroscience 2022Quote: ... with the standard-sensitivity RNA chip or an Agilent 2100 Bioanalyzer system with the RNA 6000 Nano Labchip Kit (Agilent Biotechnologies, Palo Alto, USA) was used to analyze the extracted RNA samples ...
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bioRxiv - Genomics 2022Quote: ... The obtained library quality and concentration was assessed by monitoring the distribution of the fragment sizes with a Bioanalyzer 2100 instrument using RNAnano reagent kit (Agilent Technologies, Santa Clara, CA). This size and quantity information was used for pooling the libraries in equimolar concentrations to normalize each library ...
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bioRxiv - Neuroscience 2022Quote: ... and RNA quality was assessed by determining RNA integrity numbers (RINs) using the Agilent 2100 Bioanalyzer system with the Agilent RNA 6000 Nano Chip Kit (Agilent Technologies, Santa Clara, California).