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Citations for Agilent :
5401 - 5450 of 6584 citations for Human Short Coiled Coil Protein SCOC ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Integrity was assessed using Agilent RNA 6000 Pico Kit on a 2100 Bioanalyzer instrument (Agilent Technologies, Santa Clara, CA, USA). RNA sequencing libraries were prepared using the Illumina Stranded mRNA prep ligation kit (Illumina ...
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bioRxiv - Biophysics 2024Quote: ... The pBAD30-ClsA (EE) G51A mutant plasmid was generated from the pBAD30- ClsA (EE) template with QuikChange II Site-Directed Mutagenesis Kit (Agilent). Two residues ...
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bioRxiv - Biochemistry 2024Quote: All of the mutations were introduced into a plasmid backbone expressing 6xHis tagged pNL4-3-derived IN by QuikChange site-directed mutagenesis kit (Agilent). The plasmids containing full-length WT and mutants were transformed into BL21 (DE3 ...
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bioRxiv - Cancer Biology 2024Quote: ... The library average fragment size and quantification was performed using Agilent Bioanalyzer High Sensitivity DNA kit (Agilent Cat. 5067-4626) and a final concentration determination was performed using NEBNext® Library Quant Kit for Illumina (New England Biolabs Cat ...
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bioRxiv - Cell Biology 2023Quote: ... labeled cRNA was prepared from 0.1 micro-g Total RNA using the Low Input Quick Amp Labeling Kit (Agilent Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... An aliquot of this chromatin was used to assess the size of the DNA fragments obtained by High Sensitivity NGS Fragment Analysis Kit (DNF-474) on a Fragment AnalyzerTM (Agilent). ChIP was performed using IP-Star® Compact Automated System (Diagenode Cat# B03000002 ...
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bioRxiv - Biochemistry 2023Quote: RNA concentration and purity were determined spectrophotometrically using the Nanodrop ND-8000 (Nanodrop Technologies) and RNA integrity and concentration were assessed using a Fragment Analyzer SS-RNA kit (Agilent). Per sample ...
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bioRxiv - Cancer Biology 2024Quote: Paraffin-embedded human HCC samples were cut into 3.5-µm thick tissue sections and processed for immunohistochemistry with the EnVision FLEX kit material (K800021-2, Agilent Dako) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: XF Cell Mito and XF Glycolysis Stress Test Kits were used to measure mitochondrial and glycolytic function on a Seahorse Extracellular Flux Analyzer (Agilent). The Seahorse microplate was coated with Cultrex (R&D Systems ...
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bioRxiv - Neuroscience 2023Quote: ... and their quality was assessed by capillary electrophoresis on the FragmentAnalyzer using the NGS High Sensitivity kit (DNF-474, Agilent). The libraries were pooled and sequenced on an Illumina NovaSeq 2000 ...
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bioRxiv - Microbiology 2023Quote: ... and a barcoding primer (unique for each sample to allow for demultiplexing of reads) with the Easy A cloning kit (Agilent). A 200–400 bp product was gel-purified and sequenced on an Illumina NextSeq Platform (in lab ...
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bioRxiv - Microbiology 2023Quote: ... This DNA was then subject to an initial round of PCR amplification with primers oTB535 and oTB536 with the Easy A cloning kit (Agilent), digested with NotI (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... Transfer of the Gateway cassette from these two entry vectors to the pBIFCt-2in1-NN destination vector 57 was performed through LR clonase II kit (Agilent).
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bioRxiv - Biochemistry 2022Quote: ... The quality of total RNA was examined using a fragment analyzer system with an Agilent HS RNA kit (Agilent Technologies). Libraries were prepared using an MGIEasy RNA Directional Library Prep Set (MGI Tech Co. ...
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bioRxiv - Microbiology 2023Quote: ... and pKTP109 (catR, Para::ftsI) by using the QuickChange site-directed mutagenesis kit according to the manufacturer’s instruction (Agilent Technologies). The list of primers used in this study was previously published.
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bioRxiv - Cancer Biology 2023Quote: The metabolic profile of 1205Lu melanoma cells was assessed using the Seahorse XF Cell Energy Phenotype Test Kit (Cat no.103325-100, Agilent). Both parental and NKX2.2 KO 1205Lu cells ...
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bioRxiv - Cell Biology 2023Quote: ... The tau P301L mutants were generated by PCR using QuickChange Lightning Site-Direct mutagenesis kit (Agilent Technologies, Santa Clara, USA). For all intermolecular sensors ...
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bioRxiv - Neuroscience 2023Quote: ... RNA integrity of brain organoids and mouse tissue was evaluated by analysis on the 2100 Bioanalyzer RNA 6000 Pico Chip kit (Agilent) using the RNA Integrity Number (RIN) ...
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bioRxiv - Cancer Biology 2023Quote: ... Staining visualisation was performed by incubating with a cocktail of anti-mouse and anti-rabbit secondary antibodies conjugated to horseradish peroxidase (Envision Kit, Agilent) for 60 minutes at room temperature ...
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The effects of caloric restriction on adipose tissue and metabolic health are sex- and age-dependentbioRxiv - Physiology 2023Quote: ... and RNA Integrity Number (RIN) was assessed using Agilent RNA 6000 Pico Kit and an Agilent 2100 bioanalyzer (Agilent, USA). Library preparation and sequencing was performed by BGI (Shenzhen ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA was extracted using the same kit according to manufacturer’s instructions and quality was assessed using the Agilent 2200 TapeStation System (Agilent, G2964AA) with RNA ScreenTape (Agilent ...
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bioRxiv - Pathology 2023Quote: ... RNA Integrity Number (RIN) and DV200 were determined by BioAnalyzer electrophoresis using an RNA 6000 Pico Kit (#5067-1513, Agilent). The DV200 metric refers to the percentage of total profiled RNA fragments greater than 200bp in length ...
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bioRxiv - Systems Biology 2022Quote: ... Adherent NSCs were infected with retro-Cre-Tomato retrovirus (5μl of virus was used to infect 3x105 cells) or nucleofected with expression constructs according to instructions of the Amaxa nucleofection kit (Agilent) [28] ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Amplified and purified sequencing libraries were analyzed on an Agilent 2100 Bioanalyzer (High sensitivity DNA analysis kit, Agilent, 5067-4626), pooled in equimolar amounts (2.47 ng/μL) ...
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bioRxiv - Molecular Biology 2022Quote: ... Point mutations in the repM gene were generated using specific primers and a QuikChange Site-Directed Mutagenesis kit according to the protocol supplied by the manufacturer (Stratagene). All oligonucleotide primers used in this study are listed in Supplementary Table S2.
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bioRxiv - Cancer Biology 2023Quote: ... The stranded mRNA libraries were analyzed on a 2100 Bioanalyzer instrument following the manufacturer’s protocol “Agilent DNA 7500 kit” (G2938-90024, Agilent Technologies), diluted to 10nM and pooled equimolar into multiplex sequencing pools for paired end sequencing on the NovaSeq 6000 Illumina sequencing instrument ...
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bioRxiv - Immunology 2023Quote: ... All mutations in this study were introduced using the QuikChange II XL site-directed mutagenesis kit (Agilent Technologies, Cat #200521), KLD Enzyme Mix ...
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bioRxiv - Immunology 2022Quote: ... SARS-COV-2-Strunc variants were generated in house by site-directed mutagenesis (QuikChange Multi Site-Directed Mutagenesis Kit, Agilent) starting from synthetic DNA (Genscript) ...
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bioRxiv - Molecular Biology 2023Quote: ... and C66A triple mutation), KR5>A (contains K286A, R287A, K288A, K300A, and R301A mutations) were done using QuikChange Mutagenesis Kit (Agilent). Deletion mutants (1-280 ...
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bioRxiv - Microbiology 2023Quote: ... The resulting pHD1344tub(PAC)-mt-LAF3-Cterm3V5 plasmid was then used as a template for site-directed mutagenesis of D211 (QuikChange II kit; Agilent) using forward and reverse primers listed in Supplemental Table 1 ...
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bioRxiv - Genetics 2023Quote: ... after which DNA fragments in a range of 400-700 bp were excised and extracted using Gel Extraction Kit (A&A Biotechnology) The quality and quantity of the libraries were verified with TapeStation system (Agilent) and Qubit 2.0 fluorometer ...
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bioRxiv - Genetics 2023Quote: ... the Prab-3p::hpk-1 plasmid was used and mutations were performed with a QuikChange II XL Site-Directed Mutagenesis Kit (Agilent). All the assembled plasmids were validated by sequencing prior to microinjection ...
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bioRxiv - Developmental Biology 2023Quote: ... Quality of the final library preparation was analysed on the Bioanalyzer using the High Sensitivity DNA reagents kit and cassettes (5067-4626, Agilent). For all the steps involving purification of library intermediates ...
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bioRxiv - Microbiology 2023Quote: ... Total RNA was extracted using the ZYMO RNA purification kit and RNA integrity was confirmed by the TapeStation system (Agilent). Total RNA was used to generate the sequencing libraries using the KAPA Stranded mRNA Seq kit for the Illumina sequencing platform according to the manufacturer’s protocol (KAPA biosystems) ...
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bioRxiv - Developmental Biology 2023Quote: ... all libraries were ligated to NEBNext® Multiplex Oligos for Illumina® (Dual Index Primer Set I) and quality control was performed using a 2100 Bioanalyser High Sensitivity DNA Kit 5067-4626 (Agilent) and libraries quantified using a QubitTM Fluorometer following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... we created all other Gαi3 mutants featured in this work using a QuikChange® Site-Directed Mutagenesis Kit as per manufacturer’s protocol (Stratagene, USA). A variant that is insensitive to ADP-ribosylation by Pertussis Bordetella toxin (PTX ...
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bioRxiv - Immunology 2023Quote: ... The variable heavy chain sequence of CH42 was subcloned into a plasmid coded with an IgG1 heavy chain backbone containing the N297A 29 mutation via QuikChange Site Directed Mutagenesis kit (Agilent). Antibodies were expressed through co-transfection of heavy and light chain plasmids in Expi293 HEK cells (Thermo Fisher ...
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bioRxiv - Immunology 2023Quote: ... The total RNA quality and quantity were analyzed by Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number >7.0 ...
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bioRxiv - Immunology 2023Quote: ... Agilent Seahorse XF Cell Mito Stress Test Kit was used to perform Mitostress test in the presence of 10mM glucose (Agilent) with addition of Oligomycin ...
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bioRxiv - Neuroscience 2023Quote: ... RNA quality of the tissue sections (RIN > 7) was confirmed using the Agilent RNA 6000 Nano Kit on the Bioanalyzer 2100 system (Agilent). Visium spatial gene expression slides were processed according to manufacturer instructions (10X Genomics ...
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bioRxiv - Microbiology 2023Quote: ... The quality and quantity of RNA were determined by capillary electrophoresis using an HS RNA kit and 5200 Fragment Analyzer (Agilent). For single-end RNA sequencing (RNA-seq) ...
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bioRxiv - Pathology 2023Quote: ... and oxygen consumption rates (OCR, XF Cell Mito Stress Test) were performed with a Seahorse XF24 Extracellular Flux Analyzer and test kits (Agilent Technologies ...
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bioRxiv - Microbiology 2023Quote: ... PCR products are then purified and checked for fragment size distribution on Fragment Analyzer using HS NGS Fragment Kit (1-6000bp) (Agilent).
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bioRxiv - Evolutionary Biology 2023Quote: ... Size distribution and degradation were assessed using the Fragment analyzer (AATI) and High Sensitivity Large Fragment 50 kb Analysis Kit (Agilent). Purification steps were performed using AMPure PB beads (PacBio) ...
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bioRxiv - Cell Biology 2023Quote: ... The total RNA quality and quantity were analysis of Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number >7.0 ...
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bioRxiv - Cell Biology 2023Quote: ... Site-directed mutagenesis for Drp1 R456E and Y493A was completed to introduce each mutation into this construct individually using the QuikChange Lightning kit (Agilent) with primers from Integrated DNA Technologies (Coralville ...
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bioRxiv - Cell Biology 2023Quote: ... RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). cDNA was synthesized using SMARTer Ultra Low RNA Kit for Illumina Sequencing (634936 ...
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bioRxiv - Cell Biology 2023Quote: ... the pK2:Nβ-GFP construct was used as template for site-directed mutagenesis by PCR using the QuickChange Lightning Site-Directed Mutagenesis Kit (Agilent) following the manufacturer’s instructions with the concomitant mutagenic primers for each target base substitution (S2 Table) ...
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bioRxiv - Biophysics 2023Quote: ... derivative encoding two cysteine residues at positions 366 and 511 [constructed from plasmid pGEMD (-Cys)46 by use of site-directed mutagenesis (QuikChange Site-Directed Mutagenesis Kit; Agilent) to replace codons 366 and 511 by a codon encoding cysteine residue] ...
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bioRxiv - Microbiology 2023Quote: ... The mpox sA28 and sH2 expression plasmids were generated from the above vaccinia sA28 and sH2 constructs by site-directed mutagenesis using the QuickChange Lightning Site-Directed Mutagenesis Kit (Agilent). All the vaccinia A28 mutant constructs ...