Labshake search
Citations for Agilent :
5351 - 5400 of 5601 citations for Rat CD178 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... Variants were introduced into the wild type constructions using the Quick-change II XL site-directed mutagenesis kit (Agilent, Santa Clara, CA, USA). Presence of an abnormally spliced transcript associated with the decrease of the normal transcript was considered as “Impact on splicing” ...
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bioRxiv - Microbiology 2020Quote: ... libraries were checked for quality and size distribution using the 2100 Bioanalyzer with the High Sensitivity DNA kit (Agilent, Santa Clara, CA, USA). Library quantitation was performed by Qubit using the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... Total amounts and the integrity of RNA were assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). Transcriptomic analysis was performed by Novogene (Beijing ...
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bioRxiv - Cell Biology 2022Quote: ... were introduced into the plasmid pED-FLAG-LMAN1 and pcDNA-MCFD2-Myc separately using the QuickChange site-directed mutagenesis II XL kit from Agilent (Santa Clara, CA). FVIII mutant constructs Δ807–816 (deletion of amino acids 807-816 of FVIII ...
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bioRxiv - Cell Biology 2022Quote: ... or C166A/C180A in the GABAA receptor α1 subunit) were constructed using a QuikChange II site-directed mutagenesis Kit (Agilent Genomics, catalog #: 200523). A FLAG tag was inserted between Leu31 and Gln32 of the α1 subunit and between Asn28 and Asp29 of the β2 subunit of GABAA receptors by using QuikChange II site- directed mutagenesis ...
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bioRxiv - Genetics 2022Quote: ... sheared genomic DNA was concentrated using a 0.6X v/v ratio of AMPure PB beads and libraries were sized using a Bioanalyzer 2100 instrument with the DNA 12000 Kit (Agilent Technologies, CA, USA).
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bioRxiv - Evolutionary Biology 2022Quote: ... The quality and quantity of the RNA was verified using an Agilent RNA 6000 Nano Kit on an Agilent Bioanalyzer (Agilent Technologies, California, USA) and a Nanodrop spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2022Quote: ... Basal oxygen consumption rate and mitochondrial Oxidative Phosphorylation System (OXPHOS) parameters were measured according to manufacturer’s instructions of the Mito Stress test Kit (Seahorse Bioscience, Agilent Technologies, CA, USA). Results were normalized to protein quantified using a bicinchoninic acid-based protein assay (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... The average sample fragment length and purity was determined using Agilent High Sensitivity DNA kit and the Agilent 2100 Bioanalyzer (Agilent, Santa Clara, CA). After passing quality control measures for loading the sequencing machine ...
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bioRxiv - Plant Biology 2022Quote: ... RNA integrity and quantitation were monitored with the RNA Nano 6000 Assay Kit (Cat #5067-1511) of the Agilent Bioanalyzer 2100 system (Agilent Technologies, CA, USA). Following quality control ...
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bioRxiv - Molecular Biology 2023Quote: ... GFP-TDP-4FL was generated by introducing F147/149/229/231L mutations using site directed mutagenesis with QuickChange XL kit according to the manufacturer’s protocol (Agilent Technologies, Santa Clara, CA) into GFP-TDP-43 plasmid (gifted from CNDR).
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bioRxiv - Physiology 2023Quote: ... to alanine(s) (A) or glutamate(s) (E) by site-directed mutagenesis using the QuikChange II XL Site-Directed Mutagenesis kit (Agilent, Santa Clara, CA) of a pDUAL2-CCM(- ...
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bioRxiv - Cancer Biology 2024Quote: ... WES libraries were prepared and captured using an Agilent SureSelect Human All Exon kit (Catalog No. 5191-5735, Agilent Technologies, Santa Clara, CA). The DNA library ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA concentration and RNA integrity number (RIN) were determined using an Agilent microfluidic RNA 6000 Nano Chip kit (Agilent Technologies, Santa Clara, CA) on the 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Synthetic Biology 2022Quote: ... 5’- ACC TCC ACC ACC AGA ACC ACC TCC ACC GCT CTG AAA GTA CAG ATC CTC GGT ACC TTT ATA TAA TTC GTC CAT-3’) using the Agilent QuikChange SDM kit following the manufacturer’s instructions (Agilent, Singapore, Singapore; Cat: STR_210519). This was followed by amplifying the backbone of the plasmid using primer set Ag43BBGib_F ...
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bioRxiv - Synthetic Biology 2022Quote: ... 5’- CTT TTT CAA ATT GGG GGT GTG ACC ATG CAG CGC TAG CCG CAG C −3’) using the GeneMorph II epPCR kit (Agilent, Singapore, Cat: STR_200550). The epPCR profile is as follows ...
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bioRxiv - Neuroscience 2022Quote: ... RNA concentration and integrity was consecutively analyzed by capillary electrophoresis using a Fragment Analyser (Advanced Analytical Technologies, Inc., USA) and the Agilent RNA 6000 Pico Kit (Agilent, Cat# 5067-1513). RNA samples with RNA integrity numbers (RIN ...
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bioRxiv - Genomics 2022Quote: ... and its profile was assessed using a DNA High Sensitivity LabChip kit on an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). The library was paired-end sequenced on the Illumina HiSeq2500 (Illumina ...
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bioRxiv - Physiology 2022Quote: ... The dynamin-2 mCherry mutations A618T and S619L were constructed by site directed-mutagenesis using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies, Texas, USA). (DYN2 isoform aa ...
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bioRxiv - Biochemistry 2024Quote: ... and L325A—were created using plasmids harboring the wild type GNNV-P sequence and a QuickChange Lightning site-directed mutagenesis kit (Agilent Technologies, CA, USA). Mutations were confirmed by PCR sequencing (Genomics Inc. ...
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bioRxiv - Immunology 2024Quote: ... Total amounts and integrity of RNA were assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). Then ...
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bioRxiv - Neuroscience 2023Quote: ... Site-directed S586A mutagenesis was performed on the Kv2.1 channel using the Quick-Change XL Site-directed Mutagenesis kit (Stratagene, La Jolla, CA, USA). Kv2.1S586A mutation was confirmed by sequencing.
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bioRxiv - Molecular Biology 2024Quote: ... and the RNA integrity and concentration were assessed using the RNA Nano 6000 Assay Kit for the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). Then ...
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bioRxiv - Neuroscience 2024Quote: ... RNA concentration and quality (RIN > 8.0) was evaluated using an RNA 6000 Pico Kit and a 2100 Bioanalyzer system (Agilent Technologies, Santa Clara, California). RNA sequencing was performed by the NYU Genome Technology Center ...
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bioRxiv - Molecular Biology 2023Quote: ... The average length of the indexed cDNA libraries was determined with a 2100 Bioanalyzer using the Bioanalyzer High Sensitivity DNA kit (Agilent, cat.no.:5067-4626), concentrations were measured using a Qubit dsDNA HS Assay Kit (Thermofisher ...
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Reduction of chromosomal instability and inflammation is a common aspect of adaptation to aneuploidybioRxiv - Cell Biology 2023Quote: ... The sheared DNA samples were quantified and qualified on a BioAnalyzer system using the DNA7500 assay kit (Agilent Technologies cat no. 5067-1506). With an input of maximum ...
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bioRxiv - Developmental Biology 2024Quote: ... The quality of the RNA was measured using the Agilent 2100 Bioanalyser with the Agilent RNA 6000 Nano kit (Agilent, Cat.No.5067-1511). Only RNA with RIN value above eight was used for cDNA synthesis using the iScript cDNA synthesis kit (Biorad ...
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bioRxiv - Genetics 2024Quote: ... The quality and profile of individual libraries have been quantified and visualized using Qubit™ and the Agilent Bioanalyzer dsDNA High Sensibility Kit (Agilent Technologies, USA) respectively ...
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bioRxiv - Microbiology 2024Quote: ... The RNA quality was checked by Trinean Xpose (Gentbrugge, Belgium) and the Agilent RNA Nano 6000 kit using an Agilent 2100 Bioanalyzer (Agilent Technologies, Böblingen, Germany). Pan-Archaea riboPOOL kit from siTOOLs Biotech was used to remove the rRNA ...
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bioRxiv - Bioengineering 2024Quote: ... The library was quantified with Qubit™ 1X dsDNA Assay Kits and DNA HS chips on bioanalyzer or D5000 ScreenTape (Agilent, 5067-5588) on Tapestation (Agilent ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The quantification and qualification of RNA were assessed by the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA).
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bioRxiv - Biochemistry 2023Quote: ... pmEos3.2-N1 Munc18-1WT was then used as a template to introduce the following missense mutation R388A using QuickChange Lightning site-directed mutagenesis kit (Agilent Technologies, Cat# 210518) as per manufacturer’s instructions ...
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Epigenetic deregulation of IFN and WNT pathways in AT2 cells impairs alveolar regeneration (in COPD)bioRxiv - Cell Biology 2023Quote: ... RNA concentration and integrity were measured using the RNA Pico 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA), and only samples with RIN > 8 were included in RNA-seq ...
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bioRxiv - Molecular Biology 2023Quote: ... Quality of the RNA samples (RIN score >7) was verified on the Agilent 2100 Bioanalyzer using the RNA 6000 kit (Agilent, cat# 5067-1513). 1 µg of each RNA sample was then used for poly(A ...
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bioRxiv - Cell Biology 2023Quote: ... Total amounts and integrity of RNA were assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). Total RNA was used as input material for RNA sample preparations ...
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bioRxiv - Developmental Biology 2023Quote: ... We performed exome sequencing in the child with diabetes and his consanguineous parents and sibling using the Agilent SureSelect Human All Exon Kit (Agilent SureSelect v4, 50Mb) and next-generation sequencing (Hiseq ...
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bioRxiv - Physiology 2023Quote: ... Point mutations were generated using the QuikChange II XL Site-Directed Mutagenesis or Multi Site-Directed Mutagenesis Kit (Agilent Technologies, Cedar Creek, TX) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The integrity of the RNA samples was confirmed using the Agilent Bioanalyzer 2100 with an RNA 6000 Pico Kit (Agilent, Santa Clara, CA). All samples had an RNA Integrity Number (RIN ...
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bioRxiv - Immunology 2022Quote: ... followed by quality check (QC) and fragment size distribution using a High Sensitivity Tape station Kit (Agilent 2200, #5067-5584 and #5067-5585). The library was sequenced using the HiSeq 4000 Illumina platform.
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bioRxiv - Immunology 2022Quote: ... reverse transcription was carried out with 500ng of RNA using an Affinity Script qPCR cDNA Synthesis Kit (Agilent Technologies, Santa Clara, CA, USA) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... the libraries were checked for quality and size distribution using an Agilent 2100 Bioanalyzer and the High sensitivity DNA kit (Agilent, San Diego, USA). Quantitation of the library was performed by Qubit using the Qubit dsDNA HS Assay Kit (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... The purified DNA library was analyzed using an Agilent 2100 bioanalyzer with an Agilent DNA 1000 Kit (Agilent Technologies, Santa Clara, CA, USA) and a Qubit 2.0 with Qubit 1X dsDNA HS Assay Kits (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA concentration and quality was measured using the RNA 6000 Pico kit on the Bioanalyzer 2100 capillary electrophoresis system (Agilent, Santa Clara, CA) and stored at −80°C ...
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bioRxiv - Cell Biology 2023Quote: ... Point mutations were introduced into ATP8B2 and ATP8B4 using the QuikChange II XL site-directed mutagenesis kit (Agilent Technologies, Santa Clara, CA, USA) and/or the SLiCE cloning method (Zhang et al ...
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bioRxiv - Bioengineering 2023Quote: ... The RNA quality was evaluated using a 5200 Fragment Analyzer System and an Agilent HS RNA Kit (Agilent Technologies, Santa Clara, CA, USA). Libraries were constructed Using MGIEasy RNA Directional Library Prep Set (MGI Tech Co. ...
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bioRxiv - Cancer Biology 2023Quote: ... The total amount and integrity of RNA were assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA).
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Targeting adipocyte ESRRA promotes osteogenesis and vascular formation in adipocyte-rich bone marrowbioRxiv - Molecular Biology 2023Quote: ... The total RNA quantity and purity were analyzed by using Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA, 5067-1511). RNA-seq libraries were constructed and then sequenced using an Illumina Novaseq™ 6000 (LC-Bio Technology ...
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bioRxiv - Molecular Biology 2023Quote: ... two successive rounds of cysteine to serine mutagenesis were performed using the QuikChange Lightning Multi Site-directed Mutagenesis kit (Agilent Technologies #210513/#210515) according to manufacturer’s instructions at 1/4th scale.
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bioRxiv - Systems Biology 2023Quote: ... quality check and quantification of total RNA was done using the Agilent Bioanalyzer 2100 in combination with the RNA 6000 pico kit (Agilent Technologies, 5067-1513). Total RNA library preparation was done by introducing 500 ng total RNA into Illumina’s NEBNext Ultra II directional mRNA (UMI ...
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bioRxiv - Physiology 2023Quote: ... Quality control of the total RNA submissions was completed using the Agilent TapeStation 4200 using the RNA ScreenTape Assay kit (Agilent Technologies 5067-5576). Total RNA (500ng ...