Labshake search
Citations for Agilent :
5201 - 5250 of 6515 citations for QuantiFluo Ammonia Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2019Quote: Quality and size of the cDNA libraries were assessed using the High Sensitivity D1000 kit (Agilent, Cat No. 5067-5585) and run on Illumina Next-Seq 500 sequencing machine ...
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bioRxiv - Microbiology 2019Quote: ... site directed mutagenesis on the plasmid-borne cqsR was performed using the QuikChange™ XL Site-Directed Mutagenesis Kit (Agilent) per manufacturer’s instructions.
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bioRxiv - Cancer Biology 2019Quote: ... Single cell cDNA and RNA-Seq libraries were prepared using the Chromium Single Cell 3’ Library and Gel Bead Kit v2 (10x Genomics) and the products were quantified on the 2100 Bioanalyzer DNA High Sensitivity Chip (Agilent). Each fragment contains the 10x Barcode ...
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bioRxiv - Cancer Biology 2019Quote: ... pGL3-DARPP-32 plasmids were used as templates in PCR reactions to synthesize plasmids containing mutated ASCL1 binding sites by annealing and amplifying site-directed mutagenesis primers using QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent). Digestion with Dpn I enzyme was carried out to eliminate methylated parental plasmid strands ...
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bioRxiv - Genomics 2019Quote: ... We assessed RNA integrity with the 2100 Bioanalyzer using the Agilent RNA 6000 Pico kit (Agilent Technologies; Santa Clara, USA). All samples presented RNA Integrity Number (RIN ...
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bioRxiv - Developmental Biology 2020Quote: ... Single positions within gene sequences were modified by site-directed mutagenesis applying the QuikChange II kit according to manufacturer’s protocol (Agilent Technologies).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Transferred DNA was UV crosslinked to the membrane and hybridized with a 32P-labeled (Prime-It II Random Primer Labeling Kit, Agilent) Lpar1 probe with sequence external to the 5’ end of the targeting vector ...
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bioRxiv - Developmental Biology 2020Quote: ... To generate the Sp ΔBTD-box and the btd ΔBTD-box we used the QuickChange Site-Directed Mutagenesis Kit (Stratagene) to delete the 30 bp encoding for the BTD-box using the following primers:
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bioRxiv - Cell Biology 2020Quote: ... 2xmyc-RAB10-T73A and 2xmyc-RAB35-T72A were made by directed mutagenesis from the WT vectors using the QuickChange Lightning kit (Agilent). EGFP-RAB10 (Addgene#49472)(52) ...
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bioRxiv - Cell Biology 2020Quote: ... A wobble mutation was made at an internal KpnI site in mEos3.2 to prevent its digestion using the QuikChange II XL Site-Directed Mutagenesis kit (200521; Agilent Technologies) and following primer pair ...
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bioRxiv - Cell Biology 2020Quote: ... First-strand cDNA was synthesized by reverse transcribing 500 ng of total RNA per sample using either the AffinityScript qPCR DNA Synthesis kit (Agilent), or the LunaScript RT Super Mix Kit (NEB # E3010(S/L) ...
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bioRxiv - Cell Biology 2020Quote: ... Different point mutations were introduced into SHANK3 constructs by site-directed mutagenesis (Gene Universal) or by using mutagenic oligonucleotides and the Quick-Change II site-directed mutagenesis kit (Agilent) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... RNA was reversed transcribed with MARS-seq barcoded RT primers in a 10 μl volume with the Affinity Script kit (Agilent). Reverse transcription was analyzed by qRT-PCR and samples with a similar CT were pooled (up to eight samples per pool) ...
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bioRxiv - Plant Biology 2020Quote: ... To obtain the inward open state the mutations E162Q and D344N were introduced using the quickchange site-directed mutagenesis kit (Agilent). Transformed Saccharomyces cerevisiae (strain DSY-5 ...
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bioRxiv - Bioengineering 2020Quote: ... Site-directed mutagenesis to change SB sequences was carried out using the QuikChange Lightning Site-Directed Mutagenesis kit (Agilent technologies) and specifically designed primers.
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bioRxiv - Immunology 2019Quote: ... The libraries were analyzed for size and quantity of DNAs on a 2100 Bioanalyzer using a High Sensitivity DNA Kit (Agilent), diluted and pooled in multiplex sequencing pools ...
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bioRxiv - Molecular Biology 2021Quote: ... gambiae, a 15k-probe microarray (Agilent; A-MEXP-2196 [14], using the Agilent gene expression hybridization kit (Agilent Technologies, USA). Slides were washed according to the manufacturer’s instructions and scanned on an Agilent G2565CA microarray scanner ...
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bioRxiv - Genetics 2021Quote: ... Partition barcoded libraries were then prepared based on the Chromium Exome Solution (10X Genomics) and the exome target regions was enriched by SureSelect Human All Exon V5 Kit (Agilent) according to the protocols ...
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bioRxiv - Molecular Biology 2020Quote: ... In order to generate the mutant expression plasmids for BMAL2 we used the QuikChange Lightning Multi Site-Directed Mutagenesis Kit (210515, Agilent). The bHLH mutant was generated by converting an arginine to alanine (OaBMAL2_R88A ...
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bioRxiv - Microbiology 2021Quote: Site-directed mutagenesis of GFP-ORF57 for W292A mutant was carried out using QuikChange site-directed mutagenesis kit (Agilent technologies) as per manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... The fragment size estimation of the resulting libraries was assessed with High SensitivityTM HS DNA kit runned on 2100 Bioanalyzer (Agilent) and quantified using the QubitTM dsDNA High Sensitivity HS assay (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... The fragment size estimation of the resulting libraries was assessed with High SensitivityTM HS DNA kit runned on 2100 Bioanalyzer (Agilent) and quantified using the QubitTM dsDNA High Sensitivity HS assay (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2020Quote: ... the quality of RNA was first checked by assessing the RNA integrity number (RIN) using the Agilent RNA 6000 Nano kit (5067-1511, Agilent) and the Agilent 2100 Bioanalyzer Automated Analysis System (Agilent) ...
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bioRxiv - Plant Biology 2019Quote: ... A 600 ng aliquot of labelled cRNA was used for fragmentation and array loading (Gene Expression Hybridisation Kit, Agilent Technologies). Hybridization ...
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bioRxiv - Pathology 2021Quote: ... Cyanine-3 (Cy3) labeled cRNA was prepared from 200 ng of total RNA using the One-Color Quick Amp Labeling kit (Agilent) according to the manufacturer’s instructions ...
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BCL-XL blockage in TNBC models confers vulnerability to inhibition of specific cell cycle regulatorsbioRxiv - Cancer Biology 2021Quote: ... RNA integrity was assessed using the Agilent RNA 6000 Pico kit and Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, California) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Library quality and quantity was assessed on a Bioanalyzer using the DNA High Sensitivity Kit reagents and protocol (Agilent Technologies). The quantified libraries were pooled together at a final concentration of 100pM ...
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bioRxiv - Genetics 2021Quote: ... The HiC library was then hybridized with the custom capture baits and captured by the SureSelect XT HS and XT low input library preparation kit for ILM (Agilent). 15 Capture C libraries were made from 5 human primary melanocyte cultures (C56 ...
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bioRxiv - Pathology 2020Quote: ... RNA sample integrity was evaluated and concentration was measured using an Agilent 2100 Bioanalyzer with an RNA 6000 Pico Kit (Agilent). Libraries for 16 samples were prepared using the Ion AmpliSeq™ Transcriptome Mouse Gene Expression Kit ...
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bioRxiv - Physiology 2021Quote: RNA was checked for quality using the Agilent RNA 600 nano kit and Bioanalyser instrument (Agilent Technologies, Santa Clara, CA). Aliquots of RNA (1 µg ...
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bioRxiv - Cancer Biology 2020Quote: ... GST-CD1 DENN V226K/I228D/L235D/Y238K and GST-CD1 DENN M243R/P244R/Q362A were generated using the QuickChange lightning site-directed mutagenesis kit from Agilent Technologies ...
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bioRxiv - Systems Biology 2020Quote: ... and 1 μL was used to check the expected size (~300 bp) and the purify was verified with the Agilent High Sensitivity DNA Kit as manufacturer’s recommendation (Agilent Technologies). One μL of the diluted libraries were used to quantify as recommended in the Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... The final product was assessed for its size distribution and concentration using a BioAnalyzer High Sensitivity DNA Kit (Agilent Technologies). The libraries were pooled and diluted to 3 nM using 10 mM Tris-HCl (pH 8.5 ...
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bioRxiv - Pathology 2019Quote: Double immuno-enzymatic labelling of biopsies’ sections following the described pre-treatment was carried out by means of the EnVision peroxidase and alkaline phosphatase kits (DakoCytomation). Primary antibodies were incubated for 30 minutes at room temperature ...
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bioRxiv - Genomics 2020Quote: ... Quality of total RNA was assessed on an Agilent BioAnalyzer 2100 using the RNA 6000 Nano kit (Agilent, # 5067-1511). All samples had a RIN > 7.6 ...
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bioRxiv - Cancer Biology 2021Quote: ... S200C and S200T) were introduced by site directed mutagenesis using QuikChange Lightning Site-Directed Mutagenesis Kit according to manufacturer’s protocol (Agilent Technologies). To express PRSS2 WT and Mutants protein ...
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bioRxiv - Cancer Biology 2019Quote: ... The quality of cDNA traces was assessed by using a High Sensitivity DNA Kit in a Bioanalyzer instrument (Agilent Technologies). Tagmentation and library preparation was performed using the Nextera XT DNA Library Preparation Kit (Illumina ...
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bioRxiv - Microbiology 2019Quote: E543M chiA-CTD mutant was created using pET46chiA-CTD template DNA with a QuikChange II Site-Directed Mutagenesis Kit (Stratagene) (Table S5) ...
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bioRxiv - Molecular Biology 2020Quote: ... and then analyzed by using the Agilent Tapestation 4200 System and High Sensitivity DNA D5000 kit (Agilent Technologies, CA, USA) to determine quality and quantity of the amplicons.
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bioRxiv - Neuroscience 2019Quote: ... A D1994N mutation was introduced into the pDC511-G2019S LRRK2 by site-directed mutagenesis using the QuickChange XL kit (Stratagene) as described (32) ...
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bioRxiv - Genetics 2021Quote: ... while the domain deletions were constructs from initial cDNA constructs using the site-directed mutagenesis kit QuikChange™ (Agilent Technologies) and adequate primers (Supplemental Table S4) ...
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bioRxiv - Plant Biology 2021Quote: ... and quality (RNA integrity > 8.5) of RNA was determined using a Bioanalyzer 2100 RNA 6000 Pico Total RNA Kit (Agilent Technologies). Samples containing >5 μg of RNA in total were used for RNAseq ...
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bioRxiv - Genomics 2021Quote: ... Final quality control of the purified RNAs was performed using the Agilent 2100 Bioanalyzer with the RNA 6000 Pico LabChip Kit (Agilent) and the Qubit Fluorometer with the Qubit RNA BR (Broad-Range ...
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bioRxiv - Microbiology 2021Quote: ... The specific variants were generated by site-directed mutagenesis in pCMV6-Entry-YTHDF2 using the QuikChange II site-Directed Mutagenesis Kit (Cat# 200521, Stratagene) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and Halo-V5-WTAP mutants (D301A and D301A/D302A) were generated using the QuikChange II site-directed Mutagenesis Kit (Stratagene) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... PartB RNA was quantified and purity/quality was assessed on the 2100 Agilent BioAnlayzer using the Agilent small RNA kit (5067-1548, Agilent).
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bioRxiv - Genetics 2020Quote: ... The A>T variant was introduced to PIK3C2B-pNL construct by site-directed mutagenesis using QuickChange Lightning Site-Directed Mutagenesis Kit (Agilent) to generate PIK3C2B-A>T-pNL construct ...
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bioRxiv - Immunology 2019Quote: ... cDNA synthesis was performed using AffinityScript QPCR cDNA Synthesis kit following the manufacturer’s guidelines (Agilent Technologies, Santa Clara, CA, USA). The total reaction volume was 10 µL using 5 µL of Mastermix ...
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bioRxiv - Immunology 2019Quote: ... C-terminally HA-tagged wt IFITM3 genes in the lentiviral vector pHR-SIN cGGW PGK puro were mutagenized using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent) according to the manufacturer’s instructions using the following primers (all listed 5’ to 3’) ...
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bioRxiv - Biochemistry 2019Quote: ... Mutagenesis was performed with pHmtS-ET22b as a template using a QuickChange site-directed mutagenesis kit (Stratagene, La Jolla, USA) according to the manufacturer’s protocol ...