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Citations for Agilent :
5001 - 5050 of 6515 citations for QuantiFluo Ammonia Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... The HsMyo1e E337S TEDS site mutation was generated in pBS-HsMyo1e construct using the QuickChange kit (Stratagene; La Jolla, CA) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2019Quote: ... Isolated RNA was reverse transcribed into cDNA was by Prime Script TM RT reagent Kit with gDNA Eraser (Stratagene, Takara). Real-time PCR was performed on ABI-7500 real-time PCR machine (Applied Biosystems ...
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bioRxiv - Microbiology 2019Quote: ... For quality control libraries were analyzed with a DNA High Sensitivity Kit on a 2100 Bioanalyzer system (Agilent Technologies, USA) and again quantified on a Quantus™ Fluorometer (Promega ...
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bioRxiv - Cell Biology 2019Quote: ... The KEN-box mutant was generated in the pMT-Cyclin B1-GFP plasmid using a site-directed mutagenesis kit (Agilent), following the manufacturer instructions ...
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bioRxiv - Biochemistry 2020Quote: Desired mutations were introduced into mhsT wild-type gene via site-directed mutagenesis (Mutagenesis QuikChange Lightning Kit, Agilent Technolgies, Inc) using designed primers ...
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bioRxiv - Microbiology 2019Quote: ... Spike-in RNAs were added to 100 ng total RNA using the One-color RNA Spike-In kit (Agilent Technologies) and Cy3-labeled cRNAs were synthesized using the Low Input Quick Amp Labeling kit (one-color ...
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bioRxiv - Developmental Biology 2019Quote: ... The total RNA quantity and purity were analyzed on Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number >7.0 ...
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bioRxiv - Cancer Biology 2019Quote: ... The RCC1 domain was deleted using the Quickchange® II XL Site-Directed Mutagenesis Kit according to manufacturer’s instructions (Stratagene) using the primer sequences ...
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bioRxiv - Biophysics 2019Quote: ... The γS N14D and γS N76D were generated via site-directed mutagenesis using QuikChangeXL Kit (Agilent Technologies, Santa Clara, CA). Escherichia coli cell stocks containing the plasmid of interest were grown at 37 °C ...
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bioRxiv - Developmental Biology 2019Quote: ... were dissociated and 106 cells were used to extract total RNA following manufacturer’s instructions of the Absolutely RNA Miniprep Kit (#400800, Agilent Technologies). cDNA was synthesised with 200 ng-1 μg of total RNA using M-MLV Reverse Transcriptase (Invitrogen ...
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bioRxiv - Molecular Biology 2019Quote: Constructs for the ScSth1p bromodomain mutants (Ser1276Glu, His1272Ala, Trp1338Gln, or Tyr1290Ala/Asn1333Ala) were generated using QuikChange site-directed mutagenesis kit (Stratagene). Mutant bromodomain expression and purification were carried out as for the wild-type protein ...
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bioRxiv - Molecular Biology 2019Quote: ... m6A-mut CIRBP reporter was generated by mutating the essential C in the m6A site synonymously to T using two rounds of site-directed mutagenesis with the QuikChange Lightning kit (Agilent).
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bioRxiv - Genomics 2019Quote: ... Library preparation and target enrichment was carried out using the SureSelect XT Mouse All Exon Kit (Agilent cat.# 5190-4641) per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... Total RNA was extracted at 24 h and 120 h using mirVana Isolation Kit according to manufacturer’s instructions and quantified using TapeStation (Agilent Biotechnologies). Bulk RNA sequencing was performed on biological duplicates using Illumina sequencing (75 million reads) ...
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bioRxiv - Cancer Biology 2019Quote: ... RNA labeling was done either with a two-color Quick Amp Labeling Kit (4×44K arrays) or with a one-color Low Input Quick Amp Kit (8×60K arrays) according the supplier’s recommendations (Agilent Technologies).
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bioRxiv - Microbiology 2019Quote: ... The resulting products were then run with a Fragment Analyzer capillary electrophoresis using the DNF905 1-500 base pair kit (Agilent). Quantification was performed using the following equation ...
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bioRxiv - Genomics 2019Quote: ... or 100 ng of gDNA was assembled into exome sequencing libraries using the SureSelect Human All Exon V7 Exome kit (Agilent). DNA samples were pooled to achieve an on-bait depth of 100× for the buffy coat gDNA and 300× for the plasma cfDNA.
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bioRxiv - Microbiology 2019Quote: ... Mutation of 1380Asp and 1592Asp to Ala was performed by site-directed mutagenesis using the QuikChange Lightning Multi Site-Directed Mutagenesis Kit (Agilent) generating pTOPO-IC-DEAD.
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bioRxiv - Cancer Biology 2019Quote: ... Library preparation was performed using SureSelect Human All Exon Kit v5 (cat# 5990-9857EN, Agilent Technologies, Santa Clara, CA, USA), sequencing was performed on the Illumina Xten platform ...
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bioRxiv - Molecular Biology 2019Quote: ... the membranes were incubated with [α-32P]dCTP radioactively labelled DNA probes (Prime-IT RmT Random Primer Labelling Kit, Stratagene) overnight at 65°C ...
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bioRxiv - Biochemistry 2019Quote: ... and pZeoPTPζ-ΔD2 (the D2 deletion mutant of PTPRZ-B) was generated using pZeoPTPζ as a template with a Quikchange multisite-directed mutagenesis kit (Stratagene).
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bioRxiv - Immunology 2019Quote: ... The quality and quantity of all barcoded DNA libraries were analysed using the High Sensitivity DNA analysis kit (Agilent, UK) on the 2100 Bioanalyzer Instrument and Qubit Fluorometer (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2019Quote: ... Single-point mutants in PACRG were generated by PCR mutagenesis using the QuickChange II Site Directed Mutagenesis kit (Agilent Technologies). The pDB070.iodo.5 plasmid containing the modified tRNA and tRNA synthetase for p-iodo-L-phenylalanine incorporation was obtained from Addgene (#99397 ...
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bioRxiv - Biochemistry 2021Quote: ... RpoB E310A and RpoB E310K were made by site-directed mutagenesis of the wild type constructs using the Quikchcange II XL kit (Agilent). B ...
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bioRxiv - Biochemistry 2020Quote: ... The pcDNA3.1 Hygro-DEST_VGLL2_Ω_loop mutant was generated by use of the QuikChange Lightning Site-Directed Mutagenesis kit (Stratagene, San Diego, CA), with oligo 5’CCC TTG GAG AGA CTG CAG CTT CTA TCA GGC TCC TGT GCC 3’ (removing amino acid positions 134-149 ...
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bioRxiv - Microbiology 2021Quote: ... Quantity and quality of RNA was assessed on an Agilent 2100 Bioanalyzer using Agilent RNA 6000 Nano kit (Agilent, USA). Extracted RNA was reverse-transcribed to cDNA using SuperScript™ IV First-Strand Synthesis System (Invitrogen™ ...
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bioRxiv - Genomics 2020Quote: ... Total RNA integrity and quantity of the samples were assessed using the Agilent 2100 Bioanalyzer with the RNA 6000 Nano LabChip Kit (Agilent) and NanoDrop 1000 Spectrophotometer (Thermo Scientific).
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bioRxiv - Genomics 2021Quote: ... and the quality was assessed using an Agilent 2100 Bioanalyzer with a High Sensitivity DNA analysis kit (Agilent, 5067-4626).
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bioRxiv - Genomics 2021Quote: ... The concentration and size distribution of the immunoprecipitated DNA was measured using the Bioanalyzer High Sensitivity DNA kit (#5067-4626, Agilent). Dana-Farber Cancer Institute Molecular Biology Core Facilities prepared libraries from 2 ng of DNA ...
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bioRxiv - Immunology 2021Quote: ... RNA integrity was assessed using Eukaryote Total RNA Nano Kit on the Bioanalyzer 2100 system (Agilent Technologies, Santa Clara CA). 500 ng of RNA ...
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bioRxiv - Developmental Biology 2021Quote: ... The quality of the extracted RNA was analysed using the RNA 6000 Nano kit on a 2100 Bioanalyzer (Agilent, USA). An aliquot of 500 ng of RNA was used to prepare a polyadenylated RNA library with the TruSeq Stranded mRNA Library Prep Kit (Illumina ...
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bioRxiv - Immunology 2021Quote: ... Total RNA was amplified and Cy3 labeled by using the one-color Low Input Quick Amp Labeling Kit (Agilent Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... with an average fragment length of 625 bp (ranging from 300 to 2000 bp, measured with the Bioanalyzer High Sensitivity DNA Kit, Agilent). The prepared library was sequenced on an Illumina NovaSeq 6000 Sequencing System with S4 flow cells ...
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bioRxiv - Microbiology 2021Quote: ... and Spir-1 and K7 plasmids were used as templates for site-directed mutagenesis according to the instructions of the QuikChange Site-Directed Mutagenesis Kit (Agilent). All mutations were confirmed by DNA sequencing ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were reselected for two rounds and finally transferred using a new capillary with 30-μm tip diameter to 100-μl lysis buffer (Absolutely RNA Nanoprep Kit; Agilent). Cells of multiple collections were pooled if needed to obtain 200 cells per sample ...
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bioRxiv - Microbiology 2021Quote: ... Mutant SARS-CoV-2 expression plasmids were generated by site-directed mutagenesis using the QuikChange Lightning Multi Site-Directed Mutagenesis Kit (Agilent). Unless otherwise stated all SARS-CoV-2 spike expression plasmids were based on the Wuhan-hu-1 reference sequence 41 ...
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bioRxiv - Neuroscience 2020Quote: ... The final product was assessed for its size distribution and concentration using a BioAnalyzer High Sensitivity DNA Kit (Agilent Technologies) and loaded onto an S1 flow cell on an Illumina NovaSeq 6000 (Illumina ...
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bioRxiv - Cancer Biology 2021Quote: ... The average length of the final libraries was quantified using a Fragment Analyzer (HS NGS Fragment kit, Agilent, DNF-474) and the concentration of libraries was determined using a Qubit 1X dsDNA HS kit (Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: Total extracted RNA quality was confirmed for sequencing using the Agilent 2100 Bioanalyzer system using the RNA 6000 Pico Kit (Agilent). Bacterial and mammalian rRNA depletion ...
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bioRxiv - Biochemistry 2020Quote: ... an XhoI restriction site was introduced after DrBphP residue 513 with QuikChange Lightning Multi Site-Directed Mutagenesis Kit (Agilent Technologies). Then ...
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bioRxiv - Systems Biology 2021Quote: ... The staining was performed according to the manufacturer’s procedure with EnVision G|2 Doublestain System Rabbit/Mouse (DAB+/Permanent Red) kit (Dako/Agilent K5361) on the Dako Autostainer ...
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bioRxiv - Biochemistry 2021Quote: ... Library size and quality was assessed using the Fragment Analyzer DNF-474 High Sensitivity NGS Fragment Analysis kit (Agilent Technologies). Concentrations of each library were quantified using the KAPA Library Quantification Kit for Illumina (Roche ...
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bioRxiv - Developmental Biology 2021Quote: ... The catalytically dead mutants MMP28EA-pCS2 and MMP28EA-ΔSP/NLS-GFP-pCS2 were produced by point mutation of Glutamic acid226 to Alanine from MMP28wtpCS2 and Glutamic acid209 to Alanine from MMP28ΔSP/NLS-GFP-pCS2 using QuikChange II site-Directed Mutagenesis Kit (Agilent #200523) with the primers MMP28_E-A_fdw ...
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bioRxiv - Biochemistry 2020Quote: ... fulgidus Csx3 (Supplementary figure 1) were generated using the QuikChange Site-Directed Mutagenesis kit as per manufacturer’s instructions (Agilent Technologies) and purified as for the wild-type protein.
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bioRxiv - Biochemistry 2020Quote: Site-directed mutagenesis of BAK1 scFv coding sequence into pCANTAB6 was performed following the manufacturer’s instructions in the QuikChange Site-Directed Mutagenesis Kit (Agilent Technologies). The mutations introduced in the BAK1 or the BAK1-INS1 template sequences were ...
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bioRxiv - Biochemistry 2020Quote: ... The error-prone PCR (epPCR) library of the gene encoding qmLC/A was created with the GeneMorph II kit (Agilent) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: The FANCD2 S592A and S592D cDNAs were generated by site-directed mutagenesis of the wild type FANCD2 cDNA using the Quikchange Site-directed Mutagenesis Kit (Stratagene). The forward (FP ...
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bioRxiv - Biochemistry 2020Quote: ... The gene coding for the H349Y GlgE variant was generated from the synthesized wild-type glgE gene using a QuikChange Lightening kit (Agilent). The constructs were ligated into pET21a expression vectors (Novagen ...
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bioRxiv - Microbiology 2021Quote: ... and the Spike from the B.1.429 lineage (S13I, W152C, L452R, D614G) were generated using the QuikChange II XL site-directed mutagenesis kit (Agilent Technologies). The amino acid deletions in the full-length SARS-CoV-2 Spike expressor were generated using the Q5 site-directed mutagenesis kit (NEB) ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA integrity was evaluated with Agilent Bioanalyzer 2100 and RNA 6000 Nano Kit (Cat-No. G2939BA & 5067-1511, Agilent, Germany). RNASeq libraries were prepared using illumina® TrueSeqTM Stranded mRNA Library Prep Kit ...