Labshake search
Citations for Agilent :
4951 - 5000 of 6226 citations for Cow Protein L Isoaspartate PCMT1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... USP47 catalytic domain was mutated by changing cysteine 177 into alanine (C177A) using the sense primer (5’-gtttgcaaaaggctattcaaataggcagtcattgcttggtttactagtcc-3’) using Quickchange Lightning Site-directed Mutagenesis Kit from Agilent technologies ...
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bioRxiv - Genomics 2022Quote: RNA quality was evaluated using Agilent RNA 6000 Nano Kit on the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, USA). RNAseq libraries were prepared using TruSeq Stranded Total RNA Library Prep Gold (Illumina ...
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bioRxiv - Plant Biology 2022Quote: ... was used as a template to create different promoter mutants using the QuikChange II kit according to manufacturer’s protocol (Agilent Technologies). Mutagenic mismatch primers are listed in Tab ...
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bioRxiv - Microbiology 2022Quote: ... on a Qubit Fluorometer (invitrogen) and fragment length was assessed with an Agilent 2100 Bioanalyzer (Agilent High sensitivity DNA Kit, Agilent). Samples were equimolarly pooled and sequenced on NextSeq™ 500 High Output Kit v2.5 (75 cycles ...
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bioRxiv - Cell Biology 2022Quote: ... on a OCR and ECAR were measured using a Seahorse XF Cell Mito Stress Test kit (103015-100, Agilent Technologies) as described above.
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bioRxiv - Cell Biology 2022Quote: ... and the RNA integrity was determined with an RNA ScreenTape Kit on the TapeStation 2200 system (Agilent, Santa Clara, CA). cDNA libraries were prepared from RNA extracts with a minimum RNA integrity number (RIN ...
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bioRxiv - Cell Biology 2022Quote: ... The sequencing library was determined by an Agilent 2100 Bioanalyzer using the Agilent DNA 1000 Chip Kit (Agilent, #5067-1504). Differentially expressed genes (DEGs ...
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bioRxiv - Cell Biology 2022Quote: The quantity and quality of RNA samples were analyzed using Agilent RNA 6000 Nano kit with the bioanalyzer from Agilent. RNA samples with RNA Integrity Number (RIN ...
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bioRxiv - Neuroscience 2024Quote: ... Final library concentration was assessed by Qubit dsDNA HS Assay Kit (Thermo-Fischer Scientific) and fragment size was checked using Tapestation High Sensitivity D1000 (Agilent) to ensure that fragment sizes were distributed normally about 500 bp ...
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bioRxiv - Neuroscience 2024Quote: ... Point amino acid substitutions in genes encoding GluN subunits were performed using the QuikChange site-directed mutagenesis kit (Agilent Technologies) and verified by DNA sequencing.
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bioRxiv - Neuroscience 2024Quote: ... Purified RNA was quantified using a NanoDrop spectrophotometer and RNA quality was further checked with an Agilent 2100 Bioanalyzer using the RNA nano kit (Cat Nº 5067-1511, Agilent). Only samples with RIN>9 were used for library preparation ...
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bioRxiv - Neuroscience 2024Quote: ... The Lgmn mutation leading to an inactive LGMN C191A variant40,68 was introduced by site-directed mutagenesis using the QuickChange Site-Directed Mutagenesis Kit (# 200519, Agilent Technologies) according to manufacturer’s instruction ...
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bioRxiv - Plant Biology 2024Quote: ... with the NucleoBond HMW DNA kit (Macherey Nagel, Düren, Germany) and DNA quality was assessed by capillary electrophoresis (Agilent FEMTOpulse). For PacBio library preparation ...
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bioRxiv - Physiology 2024Quote: ... Total RNA was amplified and Cy3-labeled by using the one-color Low Input Quick Amp Labeling Kit (Agilent Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2024Quote: ... Site-directed mutagenesis of this clone was conducted to generate a genomic clone of NRL5P335L using the QuikChange II site-directed mutagenesis kit (Agilent) following the manufacturer’s instructions ...
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Mutations in HUA2 restore flowering in the Arabidopsis trehalose 6-phosphate synthase1 (tps1) mutantbioRxiv - Plant Biology 2024Quote: ... RNA integrity was assessed using the RNA Nano 6000 Assay Kit on the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). RNA-seq libraries were generated with three independent biological replicates and sequenced on the Illumina NovaSeq platform by Annoroad Gene Technology ...
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bioRxiv - Plant Biology 2024Quote: ... Fragmentation (at 60 °C) and hybridization (at 65° C for 16 hours) were done using the Gene Expression Hybridization kit of (Agilent Technologies ...
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bioRxiv - Microbiology 2024Quote: To detect changes in neutralisation potency single point mutations were introduced into env encoding plasmids for PV production using the QuikChange Lightning Site-Directed Mutagenesis (SDM) kit (Agilent) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: Mitochondrial bioenergetics of MDMs and DCs were measured using the Seahorse XF Cell Mito Stress Test Kit and the XF24 Extracellular Flux Analyzer (Agilent), as previously described (100) ...
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bioRxiv - Cell Biology 2024Quote: ... Point mutations in Cebpa p42 were introduced by site-directed mutagenesis using the QuikChange Site-Directed Mutagenesis Kit (Stratagene #200518). Primers used for single mutant exchange were ...
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p110α-dependent hepatocyte signaling is critical for liver gene expression and its rewiring in MASLDbioRxiv - Cell Biology 2024Quote: ... cyanine-3 (Cy3) labeled cRNA was prepared from 200 ng of total RNA using the One-Color Quick Amp Labeling kit (Agilent) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... One microgram or more of mouse genomic DNA from each sample was analyzed by whole exome sequencing using the SureSelectXT Mouse All Exon kit (Agilent), followed by next generation sequencing using the NovaSeq 6000 S4 flow cell (Illumina ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries were then quantified using Qubit 1x dsDNA HS Assay Kit for concentration and D1000 ScreenTape (Agilent Technologies, 5067-5582) for length ...
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bioRxiv - Cancer Biology 2024Quote: ... 10X-barcoded full-length cDNA was then amplified by PCR and analysed using Bioanalyzer High Sensitivity DNA kit (5067-4626, Agilent). Up to 50 ng of cDNA was carried over to construct gene expression libraries and was enzymatically fragmented and size-selected to optimize the cDNA amplicon size prior to 5’ gene expression library construction ...
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bioRxiv - Molecular Biology 2024Quote: ... The RNA Integrity Number (RIN) for all samples was 10 as assessed by an RNA Nano Kit (Agilent #5067-1511) on an Agilent Bioanalyzer ...
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bioRxiv - Molecular Biology 2023Quote: ... Site directed mutagenesis of pORIPS and pGII was performed by PCR using the QuikChange II Site-directed mutagenesis kit (Agilent).
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bioRxiv - Molecular Biology 2024Quote: Primary mouse AT2s were isolated and plated as described above with measurement of mitochondrial respiration performed using the Seahorse XF Cell Mito Stress Test Kit (Agilent) or the Seahorse XF Palmitate Oxidation Stress Test Kit (Agilent ...
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bioRxiv - Molecular Biology 2024Quote: ... at a ratio of 0.8:1 beads:cDNA (20 μl beads:25 μl cDNA for Smart-seq2 and 8 μl beads:10 μl cDNA for Smart-seq3) and inspected on a Bioanalyzer 2100 (High Sensitivity DNA kit, Agilent).
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bioRxiv - Evolutionary Biology 2024Quote: ... Final libraries were size-selected on Fragment Analyzer using the high-sensitivity Genomic Fragment Analyzer Kit (Agilent, Santa Clara, USA), quantified using the Fluoroskan plate fluorometer (Thermo Fisher Massachusetts ...
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bioRxiv - Neuroscience 2023Quote: ... Patient M03’s DNA was captured with the SureSelect Human All Exon V6 Kit (Agilent Technologies, Santa Clara, CA, USA) and sequenced on a NextSeq500 (Illumina ...
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bioRxiv - Microbiology 2024Quote: ... Library size was validated by using Fragment Analyzer 48-Capillary Array with the HS NGS Fragment Kit (1-6000bp) (Agilent). Libraries were sequenced with a Nextseq 2×150 pb paired-end reagent kit (NextSeq 500/550 High Output Kit v2.5 ...
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bioRxiv - Microbiology 2023Quote: ... and quantity of both total and PolyA+ RNA samples were assessed using the Agilent 2100 Bioanalyzer with the RNA 6000 Nano LabChip Kit (Agilent), the NanoDrop 1000 Spectrophotometer (ThermoFisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... four sequential site-directed mutagenesis reactions were performed following the QuikChange II XL Site-Directed Mutagenesis Kit protocol (Agilent, 200521). The following primers were generated using Agilent’s QuikChange Primer Design tool ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). The mRNA enrichment ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were washed twice with PBS-Tween and incubated for 30 minutes at room temperature with the Rabbit/Mouse EnVision kits (K4001/K4007, Dako). Slides were counterstained with hematoxylin and ...
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bioRxiv - Genomics 2023Quote: ... Mean length and library concentration was determined by running libraries on a 2100 bioanalyzer using a DNA 1000 Kit (Agilent). RNA-seq libraries were sequenced by the Norwegian Sequencing Center (Oslo ...
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bioRxiv - Immunology 2024Quote: ... The resulting libraries tagged with unique dual indices were checked for size and quality using the Agilent High Sensitivity DNA Kit (Agilent). Library concentrations were measured using the KAPA SYBR Fast qPCR kit and loaded for sequencing on the Illumina NovaSeq 6000 instrument (paired-end 2X150 ...
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bioRxiv - Plant Biology 2024Quote: ... Quantitative real-time PCR was performed with primers (Table S2) and Brilliant Sybr Green QPCR Reagent Kit (Stratagene, Waldbronn, Germany). The ratio of G ...
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bioRxiv - Cell Biology 2024Quote: Whole exome sequencing on genomic DNA of the patient was performed using the SureSelect Human All Exon V6 kit (Agilent) on an Illumina HiSeq 4000 sequencer ...
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bioRxiv - Neuroscience 2023Quote: Point mutations were introduced into Stx1A or Stx2 using a TA cloning vector with the QuickChange Site-Directed Mutagenesis Kit (Stratagene). Chimeric constructs of both syntaxin isoforms were obtained using Gibson Assembly (NEB) ...
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bioRxiv - Cell Biology 2024Quote: ... Mitochondrial energetics was measured using a Seahorse XFe96 extracellular flux analyzer and the XF Cell Mito Stress kit (Seahorse Bioscience) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... pcDNA3.1zeo+/ nFlag-cmScarlet-hRobo1 was generated by inserting the Flag tag sequence at the N-terminus after the signal peptide sequence of Robo1 with QuikChange II Site-Directed Mutagenesis Kit (Agilent Technologies ...
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bioRxiv - Immunology 2024Quote: ... residues D141 of Regnase-1 and D252 of Regnase-3 were mutated to asparigines using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent) following manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: The quality of the cDNA and resulting sequencing libraries were checked by bioanalyzer (High Sensitivity DNA Kit, Agilent, 5067-4626). The libraries were sequenced using Illumina NovaSeq PE150 ...
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bioRxiv - Genetics 2024Quote: Membranes were hybridized with “probe 4” for the HIS4::LEU2 hotspot 100 which was 32P-dCTP labelled using the Prime-It II Random Primer Labeling Kit (Stratagene). Following hybridization ...
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bioRxiv - Genetics 2024Quote: ... The SCN5A mutations E171Q (Ensembl transcript ENST00000333535 c.511G>C) and R222Q (c.665G>A) were introduced with a QuikChange Multi Lightning kit (Agilent) with primers 5’ttcaccgccatttacacctttCagtctctggtca (E171Q ...
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bioRxiv - Microbiology 2024Quote: ... The plasmids expressing the BA.5 spike with the A570S mutation or the A570S/K147E double mutation were obtained by site-directed mutagenesis using a QuikChange II Site-Directed Mutagenesis Kit (Agilent). The dual split protein (DSP ...
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bioRxiv - Immunology 2024Quote: ... Mitochondrial stress test assay for pDC on the XF-HS Mini was optimized in-house within the ranges provided by the manufacturer and performed using the mitochondrial stress test kit (Agilent), with the addition of 2-DG (Millipore Sigma ...
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bioRxiv - Immunology 2024Quote: ... and RNA integrity was assessed with Agilent RNA 6000 nano kit (Aligent, Cat. No. 5067-1511) using an Agilent 2100 bioanalyzer (Agilent). The average A260/280 ratio was 1.9 (range 1.8-2.1) ...
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bioRxiv - Immunology 2024Quote: ... RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). ii ...