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Citations for Calbiochem :
101 - 150 of 245 citations for Hyaluronidase PH 20 SPAM1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... the cell pellet was lysed by sonication in lysis buffer (2mM Tris–HCl pH 7.4, 0.5M NaCl, 20mM imidazole, 10% glycerol, Protease Inhibitor Cocktail Set III Calbiochem, 1:10,000) and centrifuged (10,000g ...
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bioRxiv - Genetics 2020Quote: ... 20 μg/mL hemin chloride and 40 μg/mL G418 disulfate (Calbiochem). Single colonies appeared 7-10 days after transfection and clonal parasites were subsequently transferred to liquid media ...
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bioRxiv - Neuroscience 2023Quote: ... cells were pretreated for 20 min with cyclosporine (CsA) or VIVIT (Calbiochem). After 24 h of incubation at 37°C with 5% CO2 ...
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bioRxiv - Developmental Biology 2023Quote: ... 20 µM PD98059 and 10 µM SP600125 (Calbiochem #559389, #513000 and #420119) or vehicle (DMSO ...
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bioRxiv - Neuroscience 2020Quote: ... Anti-GluR1 Antibody (Calbiochem-PC246 ...
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bioRxiv - Cell Biology 2021Quote: 20 mg of mitochondria were lysed with digitonin (Calbiochem; 6.6 g/g protein) for 15 min on ice with occasional vortexing and cleared from insoluble material for 20 min at 20,000 × g at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... or JNK inhibitor II (also known as SP600125) (20 μM) purchased from Calbiochem, for 24 hours ...
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bioRxiv - Immunology 2019Quote: Total Cell lysates were prepared using NP40 lysis buffer (150mM NaCl, 0.5%NP40, 50mM Tris, pH 8.0) containing protease inhibitors (Protease V cocktail, Calbiochem or Halt-protease, Invitrogen), 1mM sodium orthovanadate (Sigma) ...
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bioRxiv - Neuroscience 2021Quote: ... we incubated [APOE4/E4]-Ki astrocytes with 100 nM of a small molecule structure corrector for APOE4 named PH-002 34 (Calbiochem, Sigma-Aldrich) during 24 hours ...
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bioRxiv - Cancer Biology 2020Quote: ... dipped in MilliQ, and mounted in Mowiol (0.1 M tris-HCl pH 8.5, 25% glycerol, 10% Mowiol (Calbiochem, Merck Millipore, Darmstadt, Germany)) ...
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Myosin VI regulates ciliogenesis by promoting the turnover of the centrosomal/satellite protein OFD1bioRxiv - Cell Biology 2021Quote: ... pH 7.5, 250 mM NaCl, 1 mM EDTA, 5% Glycerol, 0.1% NP40, Protease Inhibitor Cocktail set III, Calbiochem, and 0.1 mM PMSF). Sonicated lysates were cleared by centrifugation at 16,000 rpm for 30 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... broad spectrum matrix metalloproteinase (MMP) inhibitor GM6001 (Ilomastat; 20 μM, unless stated otherwise; Calbiochem) was added to both collagen-cell suspensions and supernatants.
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bioRxiv - Cell Biology 2020Quote: ... at room temperature for 20 minutes and mounted with Moviol (Calbiochem, La Jolla, CA) containing 5 μg/ml DAPI (Thermo Scientific/Molecular Probes ...
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bioRxiv - Cancer Biology 2021Quote: Antibodies used: BRCA1 (Calbiochem OP93), RAD51 (H-92 ...
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bioRxiv - Neuroscience 2022Quote: ... APLP2 C-terminal antibody (Calbiochem).
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bioRxiv - Cell Biology 2022Quote: ... HRP-conjugated secondary antibodies (Calbiochem), HRP-conjugated anti-rabbit secondary antibody ...
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bioRxiv - Cell Biology 2023Quote: ... anti-Trp1 antibody (TA99, Calbiochem), anti-ZO-1 antibody (33-9100 ...
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bioRxiv - Molecular Biology 2023Quote: ... and GAPDH antibody (Calbiochem; CB1001), as a housekeeping gene antibody.
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bioRxiv - Molecular Biology 2023Quote: ... the cells were solubilized for 1 h at 4□°C in buffer (50□mM HEPES-NaOH [pH 7.5], 300□mM NaCl, 2% (w/v) N-dodecyl β-d-maltoside (DDM, Calbiochem, San Diego, CA), protease inhibitor cocktail ...
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bioRxiv - Developmental Biology 2023Quote: ... The anti-She antibody was from Novus and the anti-actin antibody was from Calbiochem. Fixed cultures were stained with 0.1% toluidine blue ...
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bioRxiv - Developmental Biology 2019Quote: ... They were finally washed with 0.1% Tween 20 in PBS and mounted in Mowiol (Calbiochem). Images were acquired with an Axiovert 200M microscope fitted with an Axiocam or Axiocam 305 camera (Carl Zeiss ...
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bioRxiv - Cell Biology 2020Quote: ... Secondary antibodies conjugated to HRP (Calbiochem) were used at appropriate dilution ...
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bioRxiv - Biochemistry 2021Quote: ... GAPDH antibody (#CB1001500UG) was from Calbiochem. Antibodies against p62 (#5114) ...
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bioRxiv - Immunology 2020Quote: ... Anti-actin monoclonal antibody from Calbiochem-Merck Chemicals Ltd ...
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bioRxiv - Neuroscience 2021Quote: ... the brain was dissected and homogenized in RIPA extraction buffer (10mM HEPES/NaOH pH 7.4, 150mM NaCl, 1% Triton X-100, 1% SDS) with protease inhibitors (Calbiochem Protease Inhibitor Set V EDTA-free). Western blot analysis was performed with Bio-Rad wet transfer system and PVDF membrane (Millipore) ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed with ice-cold PBS and lysed with ice-cold IP buffer (10 mM HEPES pH 7.4; 150 mM NaCl, 1% Triton X-100, plus protease inhibitor cocktail Set I-Calbiochem 1:100 (Cat# 539131, Merck Millipore), 1 mM Na3VO4 and 1mM NaF) ...
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bioRxiv - Cell Biology 2022Quote: ... or kinase domain ERK3 in kinase buffer (250 mM HEPES pH 7.5, 500 mM NaCl, 25 mM MgCl2, supplemented with protease inhibitor cocktail Set I-Calbiochem 1:100 (Cat# 539131, Merck Millipore) and phosphatase inhibitors ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... PamChip arrays (PamGene #88101) were blocked for 20 cycles with 1% Bovine serum albumin (Calbiochem #126609) in Tris-buffered saline (TBS) ...
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bioRxiv - Cell Biology 2023Quote: ... we inhibited non-muscle myosin II ATPase with (-)-Blebbistatin (Blebb) (cat#20-339-11MG, MilliporeSigma Calbiochem) in CAD cells and primary hippocampal neurons ...
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bioRxiv - Developmental Biology 2020Quote: Embryos were washed in PBS with 0.1% BSA and 0.1% Tween-20 (PBST; Calbiochem, San Diego, CA) and fixed with 4% paraformaldehyde (Alfa Aesar ...
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bioRxiv - Biochemistry 2022Quote: Neuro2a cells were grown on glass coverslips and incubated with 20 μM nocodazole (Calbiochem, San Diego CA). Coverslips were removed at zero time and 2 hours and were fixed for 10 min using 3% formaldehyde in PBS prior to indirect immunofluorescence analysis as described below ...
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bioRxiv - Molecular Biology 2019Quote: ... ACV was removed and cultures were induced to reactivate by treatment with LY294002 (20 μM, 20hrs, Calbiochem), NU7441 (1 μM ...
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bioRxiv - Biochemistry 2023Quote: The peptidase activity of the 20S proteasome was measured with commonly used fluorogenic peptide substrates (Bachem, Calbiochem): succinyl-LeuLeuValTyr-4-methylcoumarin-7-amide (SucLLVY-MCA ...
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bioRxiv - Microbiology 2023Quote: ... cells were pretreated 1 h at 37°C with 20 nM bafilomycin A1 (Calbiochem, stock in DMSO) to block endocytosis ...
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bioRxiv - Neuroscience 2021Quote: ... List of Primary Antibodies used: Tubulin (Calbiochem) 1:5,000 ...
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bioRxiv - Molecular Biology 2021Quote: ... using an anti-FLAG antibody (Calbiochem, USA) (Kumari et al. ...
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bioRxiv - Genomics 2023Quote: ... For immunoprecipitation antibody against MPO (Calbiochem, 475915) was used for both treated and untreated cells ...
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bioRxiv - Neuroscience 2019Quote: ... Fyn was IPed with 1µg Fyn antibody (mouse monoclonal antibody [mAb], sc-434, SantaCruz) and 10 µL Protein G plus-agarose (CalBiochem). Washed Protein G bead pellets were resuspended in 2X Laemmli buffer and subjected to Western blot analysis ...
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bioRxiv - Plant Biology 2021Quote: ... The chromatin complexes were immunoprecipitated with anti-GFP antibody (ab290) and an anti-myc mouse antibody (mAb 9E10, Calbiochem) with a concentration of 2 μg / sample and incubated in ChIP dilution buffer with 20 μL of Dynabeads Protein A (Thermofisher ...
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bioRxiv - Genetics 2021Quote: ... https://www.oeaw.ac.at/gmi/research/research-groups/ortrun-mittelsten-scheid/resources/) with or without 20 µg/ml hygromycin B (Calbiochem) and grown for 14 d under long day (LD ...
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bioRxiv - Cell Biology 2020Quote: ... Primary antibodies were: GAPDH (clone 6C5, #CB1001, Calbiochem), p16A (ArpC5A ...
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bioRxiv - Cancer Biology 2022Quote: ... PAX3 (Rabbit Polyclonal Antibody Cat. No. CA1010, Calbiochem), GLI1 (Rabbit Polyclonal Antibody ...
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bioRxiv - Neuroscience 2022Quote: ... the cells were then incubated overnight at 4°C with primary antibodies (rabbit anti-AUTS2, 1:200, Atlas Antibodies; rabbit anti-GRIA1, 1:10, Calbiochem; mouse anti-GRIA2 ...
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bioRxiv - Neuroscience 2021Quote: ... nuclear labeling with Hoechst was developed for 20 minutes before washing with 0.1M PBS and mounting with MowiolTM (475904, Calbiochem). For primary cultures of CH or PSB CR cells growing on (PAA ...
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bioRxiv - Microbiology 2019Quote: ... BCBL-1 B cells were treated with vehicle (untreated) or stimulated with 20 ng/ml TPA (in DMSO) and Sodium Butyrate (NaB) 0.3 mM (Calbiochem) (in water ...
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bioRxiv - Cell Biology 2022Quote: Cells were seeded in 6 well plates and arrest in G2 for 20 h with 10 μM RO3306 (Calbiochem) addition ...
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bioRxiv - Bioengineering 2022Quote: ... The microfluidic devices were then washed three times with PBS and permeabilized for 20 min with 0.1% Triton X-100 (Calbiochem) diluted in PBS ...
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bioRxiv - Biophysics 2023Quote: ... or FGFJI (20 ng ml−1, bFGF, R&D Systems; and 200 nM JAK inhibitor, Calbiochem, Merck Millipore, Germany). Cells were collected for mechanical characterization and RNA-Seq after 5-day exposure to the respective culture conditions (30).
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bioRxiv - Microbiology 2019Quote: ... The blots were washed with PBS and incubated with primary antibodies (anti-EBV gH/gL (a rabbit polyclonal antiserum;1:200) (45) and anti-His tag antibody (OB05, Calbiochem, 1:1000) for His-tagged KSHV gL ...
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bioRxiv - Plant Biology 2020Quote: ... secondary antibody: anti-rabbit HRP (1:10,000, Calbiochem #401353)).