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Citations for Calbiochem :
51 - 100 of 245 citations for Hyaluronidase PH 20 SPAM1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... Z-VAD-fmk (20 μM, 8hrs, Calbiochem) was used to block reactivation and was used concomitantly with Etoposide (8hrs ...
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bioRxiv - Microbiology 2019Quote: ... 20 ng/ml low-density lipoproteins (Calbiochem), penicillin/streptomycin ...
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bioRxiv - Cell Biology 2022Quote: ... 20 μM of 1NA-PP1 (Calbiochem, 529579) was washed in soon before P0 cytokinesis completion ...
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bioRxiv - Biophysics 2023Quote: ... 20 U/mL cholesterol oxidase (Calbiochem, Millipore), or 10 U/mL sphingomyelinase (Calbiochem ...
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bioRxiv - Developmental Biology 2023Quote: ... 20 µM VEGFR2i-III inhibitor (676487, Calbiochem), 0.3 µM Cytochalasin D (C8273 ...
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bioRxiv - Cell Biology 2022Quote: ... pH 6.8) enriched in protease inhibitor R cocktail set III (EDTA free; 539134; Calbiochem) and phosphatase inhibitors (cocktail 2 ...
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bioRxiv - Cancer Biology 2019Quote: ... pH 7.4) containing protease inhibitor cocktail set I (Calbiochem-Novabiochem Corp., San Diego, CA, USA). The collected lysate proteins were then subjected to immunoblotting with LC3 (L7543 ...
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bioRxiv - Microbiology 2020Quote: ... pH 7.2) or EC-BA buffer (EC buffer supplemented with 50 mM BAPTA-AM (Calbiochem)) at 370C for 15 mins with or without inhibitors as required ...
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bioRxiv - Molecular Biology 2019Quote: ... RPA2 (Calbiochem NA19L, clone RPA34-20, 1:1000); H2AX pS139 (γH2AX ...
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bioRxiv - Molecular Biology 2019Quote: ... JNK inhibitor (20 μM, JNK Inhibitor II, Calbiochem) was used to block reactivation and was used for pre-treatment (6 hrs ...
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bioRxiv - Neuroscience 2020Quote: ... or 5% milk and 0.1% tween 20 (Calbiochem) in PBS depending on the primary antibody utilized ...
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bioRxiv - Cell Biology 2020Quote: ... cells were released into MG132 (Calbiochem, 20 μM) for 2.5 h then fixed with PTEMF (0.2% Triton X-100 ...
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bioRxiv - Cancer Biology 2022Quote: ... 20 μl of MTT (5 mg/ml, Calbiochem) solution was added to each well and incubated for 2 h ...
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bioRxiv - Neuroscience 2020Quote: ... 5 μg/ml RNAse A pH 7.4 and protease inhibitor cocktail set III EDTA-free (Calbiochem) added of 1 mM PMSF ...
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bioRxiv - Cell Biology 2020Quote: ... 20 μmol/L JNK inhibitor SP600125 (Calbiochem, Darmstadt, Germany) and 100 μmol/L PE treatment ...
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bioRxiv - Genomics 2019Quote: ... for 2 min or 20 µg/mL lactimidomycin (Calbiochem) for 30 min to media prior to PBS wash and lysis ...
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bioRxiv - Microbiology 2020Quote: ... the cells were treated with 20 μM MG132 (Calbiochem) for 8 h ...
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bioRxiv - Neuroscience 2019Quote: ... with or without 20 µM glutamate (Calbiochem Cat #3510) for a final glutamate concentration of 10 µM ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were again washed with PBS pH 7.4 (3 × 10 min) and mounted in antifade (Mowiol, Calbiochem).
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bioRxiv - Biochemistry 2021Quote: ... whereupon the final two wash steps were replaced with a single wash in 1.0 ml of 5 mM sodium phosphate buffer (pH 7.4) containing 0.1% (w/v) sarkosyl (Calbiochem) and 0.3% (w/v ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were washed with ice cold PBS and lysed in IP lysis buffer (50 mM Tris, pH 7.5, 10 mM NaCl, 2.5 mM MgCl2, 1 mM DTT, 0.01% digitonin [Calbiochem 300410] and protease inhibitors ...
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bioRxiv - Cell Biology 2020Quote: ... 15 and 20 μM IKK-2 inhibitor IV (Calbiochem, #401484) for 6 hr in a serum-free medium ...
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bioRxiv - Neuroscience 2020Quote: ... BACE inhibitor C3 (20 μM; Calbiochem, Sigma-Aldrich Cat# 565788), Begacestat (1 μM ...
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bioRxiv - Microbiology 2019Quote: ... 20 µL protein A agarose suspension (Calbiochem/Millipore #IP02-1.5ML) was added to each and samples were rocked at 4°C for 2 hours ...
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bioRxiv - Microbiology 2022Quote: ... and DNase I treated (Calbiochem; 20 µg/ml of supernatant) in the presence of 10 mM magnesium chloride (MgCl2 ...
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bioRxiv - Cell Biology 2019Quote: ... 20 µM of SU5416 and 40 µM of SU5402 (Calbiochem) were chosen for treatment of regenerating hydra ...
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bioRxiv - Neuroscience 2023Quote: ... mouse anti-APC (Calbiochem, OP80, 1:20, with antigen retrieval), and goat anti-IBA1 (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... and coverslipped with antifade medium (FluorSave™, Calbiochem, #345789–20). A negative control (incubated only with the secondary antibody ...
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bioRxiv - Biochemistry 2022Quote: ... ScDIP2-GFP knock-in cells were resuspended in a cell resuspension buffer (50 mM Tris/HCl, pH 7.6, 0.6 M sorbitol, 1mM EDTA) containing protease inhibitor cocktail (Calbiochem) and 1mM PMSF (Sigma) ...
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bioRxiv - Biophysics 2022Quote: ... were mixed in 70 μL of assembly buffer (50 mM HEPES-KOH, pH 7.5, 300 mM KCl, 5 mM MgCl2, 2 mM TCEP, 4% glycerol, 2 mM ATPγS [Calbiochem]) for 5 min at 25 °C ...
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bioRxiv - Biochemistry 2020Quote: Pelleted cells were resuspended in 30 mL Trit buffer (PBS pH 7.4 supplemented with 150 mM NaCl, 0.05% Triton X100) and Protease Inhibitor Cocktail (Calbiochem). Cells were lysed on ice with sonication after which DNAse I (1 μL ...
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bioRxiv - Neuroscience 2020Quote: ... Total mouse hippocampus from the left hemisphere was homogenised using a mechanical homogeniser and disposable pestles (Anachem) in 100μl TBS (50mM Tris-HCl pH 8.0) containing protease and phosphatase inhibitors (Calbiochem). The homogenate was then centrifuged at 4 °C for 30 mins at 16,000 × g ...
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bioRxiv - Biochemistry 2021Quote: ... Cell pellets were sonicated in 20 mM HEPES buffer pH 7.5 containing 0.25 M sucrose and 1:100 protease inhibitor cocktail set V (Calbiochem) and centrifuged at 2,000 × g for 10 min to pellet nuclear fraction ...
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bioRxiv - Immunology 2022Quote: ... Peptide loading was performed in citrate saline buffer (100 mM citrate, pH 6.0, 0.2% β-octyl-glucopyranoside (Calbiochem), 1×complete protease inhibitors (Roche) ...
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bioRxiv - Physiology 2023Quote: Total protein extracts were obtained from microalgal cells in exponential phase of growth (e.g. 4 days) in 50 mM Tris buffer pH 8.0 supplemented with protein inhibitor cocktail (539131, Calbiochem). Free-living and symbiotic microalgae were disrupted by bead beating with a Precellys device (Bertin Technologies ...
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bioRxiv - Molecular Biology 2024Quote: ... The pellet was suspended in 2 ml of lysis/binding buffer (25 mM HEPES/KOH, pH 7.6, 10 % glycerol, 150 mM KCl, 1 mM dithiothreitol, 0.1 % Elugent (Calbiochem), loaded into SHREDDER tubes (Pressure Biosciences Inc ...
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bioRxiv - Cancer Biology 2022Quote: ... Purified proteins were dialyzed against 20 mm Tris/HCl (MERCK, Calbiochem, catalogue No ...
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bioRxiv - Cancer Biology 2021Quote: ... and mouse anti-N-Myc (NCM II 100, Calbiochem, 1:20). Counterstaining ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were rinsed with cold PBS1X and then lysed in Triton-X100 buffer at 4°C (50mM HEPES pH 7.4, 150mM NaCl, 1 % Triton X-100, protease inhibitors (Calbiochem) and phosphatase inhibitor cocktails II and IV (Calbiochem)) ...
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bioRxiv - Neuroscience 2020Quote: ... A half cortex was weighed on a microscale and homogenised in 4 volumes of ice-cold Tris-buffered saline (TBS) (50mM Tris-HCl pH 8.0) containing a cocktail of protease and phosphatase inhibitors (Calbiochem) using a handheld mechanical homogeniser and disposable pestles (Anachem) ...
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bioRxiv - Neuroscience 2020Quote: ... in approximately 5 volumes of ice-cold Tris-buffered saline (TBS) (50 mM Tris-HCL, pH 8.0) containing a cocktail of protease and phosphatase inhibitors (Calbiochem). Homogenates were centrifuged at 16,000 x g at 4 °C for 30 min ...
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bioRxiv - Neuroscience 2020Quote: Weighed cortex was homogenised in Tris-buffered saline (TBS) (50 mM Tris-HCl pH 8.0) plus complete protease and phosphatase inhibitors (Calbiochem) before centrifugation at 175,000 × g for 30 minutes at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... A half cortex was weighed on a microscale and homogenised in 4 volumes of ice-cold Tris-buffered saline (TBS) (50mM Tris-HCl pH 8.0) containing a cocktail of protease and phosphatase inhibitors (Calbiochem) using a handheld mechanical homogeniser and disposable pestles (Anachem) ...
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bioRxiv - Neuroscience 2022Quote: A half cortex was weighed on a microscale and homogenised in 3 volumes of ice-cold Tris-buffered saline (TBS) (50mM Tris-HCl pH 8.0) containing a cocktail of protease and phosphatase inhibitors (Calbiochem) using a handheld mechanical homogeniser and disposable pestles (Anachem ...
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bioRxiv - Cancer Biology 2022Quote: ... collected and lysed in lysis buffer RGMT (50mM HEPES pH 7.4, 150mM NaCl, 1mM MgCl2, 1mM NaF, 1% Triton-x-100, plus protease inhibitor cocktail I [Calbiochem]). Cellular lysates were cleared by centrifugation and 225µL of total protein extract was incubated with biotin-tagged CIGB-300 (100μM ...
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bioRxiv - Developmental Biology 2022Quote: ... Tissues were homogenized in cold lysis buffer (0.1% NP-40, 50mM Tris-HCl [pH 7.2], 250mM NaCl, 2mM EDTA, phosphatase inhibitor mixture I and II [Calbiochem] and one tablet of Minicomplete [Roche] per 10ml ...
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bioRxiv - Plant Biology 2022Quote: ... except that the resuspension of protein precipitate was in modified denaturing urea buffer (8 M urea, 2% SDS, 100 mM HEPES-KOH pH 7.4, 10 mM EDTA and protease inhibitor [Calbiochem, now Merck ...
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bioRxiv - Neuroscience 2023Quote: ... Heads were snap-frozen in liquid nitrogen then homogenized using a mechanical homogenizer in 100 μL TBS (50mM Tris-HCl, pH 8.0) containing 1:200 protease inhibitor cocktail set III (Calbiochem). Homogenates were centrifuged at 16,000 g at 4°C for 30 min ...
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bioRxiv - Neuroscience 2019Quote: ... or both hippocampi from a single mouse was homogenized in 2 mL of ice-cold homogenization buffer (0.32 M sucrose, 4 mM HEPES pH 7.4, protease inhibitor cocktail EGTA-free (Calbiochem, 1:1000), RNasin (Promega ...
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bioRxiv - Developmental Biology 2020Quote: Mammalian cells were lysed using RIPA buffer (50mM Tris, pH 7.4, 150mM NaCl, 1%NP-40, 0.5%sodium deoxycholate) with protease inhibitors (Calbiochem, Billerica, MA). Protein samples were separated on 4–15%gradient TGX precast gels (Bio-Rad ...