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Citations for Calbiochem :
351 - 400 of 445 citations for BN80933 CAS 214348 10 4 98% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Cells were washed twice with 3 ml PBS at 400 g for 5 minutes at 4°C and 500 uL PI staining buffer was added (1X PBS, 50 μg/mL PI (Calbiochem), 2μg/mL RNase A (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were labeled with the indicated primary antibodies overnight at 4°C in the presence of 0.01% saponin (Calbiochem, 558255) and 1% BSA ...
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bioRxiv - Cell Biology 2022Quote: ... and AR-C118925 {5-[[5-(2,8-dimethyl-5H-dibenzo[a,d]cyclohepten-5-yl)-3,4-dihydro-2-oxo-4-thioxo-1(2H)-pyrimidinyl]methyl]-N-2H-tetrazol-5-yl-2-furancarboxamide} were obtained from Calbiochem-Merck Millipore (Billerica ...
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bioRxiv - Neuroscience 2021Quote: Pharmacological agents were used in the following concentrations and time courses: cycloheximide (Calbiochem, 0.2 μg/μl, 2, 4 or 8h), dexamethasone (Invivogen ...
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bioRxiv - Immunology 2022Quote: The caspase-1 activity was determined with the fluorometric substrate Z-YVAD 7-Amido-4-trifluoromethylcoumarin (Z-YVAD-AFC, caspase-1 substrate VI, Calbiochem), as described previously (Angosto et al. ...
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bioRxiv - Biochemistry 2021Quote: ... resuspended in 4 ml ice-cold KHM buffer supplemented with 80 μg/ml high purity digitonin (Calbiochem, cat. number 300410) and incubated on ice for 5 min to permeabilise the plasma membrane ...
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bioRxiv - Immunology 2022Quote: Caspase-1 activity was determined with the fluorometric substrate Z-YVAD 7-Amido-4-trifluoromethylcoumarin (Z-YVAD-AFC, caspase-1 substrate VI, Calbiochem) as described previously [47,48] ...
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bioRxiv - Neuroscience 2023Quote: ... O-GlcNAc transferase and O-GlcNAcase activities were blocked by 5 µM OSMI-4 (MedChem Express) and 5 µM Thiamet-G (Calbiochem) treatments ...
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bioRxiv - Biochemistry 2022Quote: ... Nuclei were stained with DAPI (1:1000 in PBS, AppliChem, Darmstadt, Germany) and coverslips were mounted on Mowiol 4-88 (Calbiochem, Merck Millipore ...
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bioRxiv - Cancer Biology 2023Quote: ... The concentration of anti-E-A14-specific Ig in plasma was estimated in comparison with a standard curve generated with anti-Her2 monoclonal antibody (Ab-4; Calbiochem).
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed 3 times with PBS and fixed to a slide using 5 μL of Mowiol (4-88 Reagent, Calbiochem). Fluorescent images were acquired with a Leica Dmi8 microscope (Leica Microsystems).
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bioRxiv - Microbiology 2024Quote: ... Viability was assessed after staining cells with 1 µg/mL propidium iodide (PI, stored at 4°C protected from light; Calbiochem). Cell number and percentage were obtained from gatings done on the Attune NxT software.
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bioRxiv - Neuroscience 2024Quote: ... was followed by primary antibody solution incubation overnight at 4 °C for detection of the following markers: monoclonal mouse: anti-APC CC-1 (1:50, Cat. No. OP80, Calbiochem); anti-MBP (1:500 ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated overnight with appropriate secondary antibodies (see Key resources table) and coverslipped with antifade mounting medium Mowiol (4-88 reagent, Calbiochem). For Mash1 staining ...
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bioRxiv - Immunology 2022Quote: Enriched mouse NK cells from Dd−/− and Dd+/+ mice were incubated with SHP-1 inhibitor (denoted in the paper as iSHP-1) (NSC-87877, CAS 56932-43-5 – Calbiochem) of different concentrations or PBS of the amount corresponding to the highest iSHP-1 concentration ...
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bioRxiv - Molecular Biology 2021Quote: ... then rinsed in PBS and placed in serum-free DMEM with 10 µM puromycin (Calbiochem) for 30 minutes at 37°C.[33] Muscles were then snap frozen in liquid nitrogen for subsequent Western blotting ...
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bioRxiv - Cell Biology 2020Quote: ... Wnt-surrogate55 (0.15 nM, U-Protein Expression) and Rho kinase inhibitor (10 μM, Calbiochem Y27632) was added to the media whenever cells were at a single-cell stage to help recovery ...
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bioRxiv - Physiology 2022Quote: ... then washed three times (each time 10 min) and subsequently mounted with Fluorsave medium (Calbiochem). The controls were the sections where primary antibody was omitted ...
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bioRxiv - Neuroscience 2024Quote: ... plates in mTesR1 supplemented with 10 μM ROCK inhibitor (Y27632, Calbiochem/ Millipore, Burlington, MA, USA). Next ...
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bioRxiv - Immunology 2022Quote: ... at a 4:1 molar ratio of monomer:streptavidin in the presence of a 1x protease inhibitor cocktail solution (Calbiochem, Millipore Sigma). 1/5th volumes of streptavidin-PE or streptavidin-BV421 were added to each monomer every 20 minutes and incubated ...
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bioRxiv - Immunology 2023Quote: ... at a 4:1 molar ratio of monomer:streptavidin in the presence of a 1x protease inhibitor cocktail solution (Calbiochem, Millipore Sigma). Briefly ...
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bioRxiv - Neuroscience 2024Quote: ... free-floating sections were incubated overnight at 4°C with a polyclonal rabbit anti-tyrosine hydroxylase (TH) antibody (Calbiochem, 657012-100μL) diluted 1:250 ...
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bioRxiv - Genomics 2023Quote: ... Conidia were harvested by washing the plates with sterile distilled water and filtered through sterile Miracloth (Calbiochem, La Jolla CA, USA) into 50 ml Falcon tubes ...
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bioRxiv - Physiology 2023Quote: ... MLEC were incubated with appropriate fluorophore-labelled secondary antibodies for 1 hour and mounted with Mowiol (Calbiochem, San Diego, CA, USA). Nuclei were visualized by DAPI staining ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with cOmplete Protease Inhibitor Cocktail (MilliporeSigma, Burlington, MA, USA) and phosphatase inhibitor cocktail set V (Calbiochem, San Diego, CA, USA). Lysates were mixed 1:1 with 2X Laemmli Buffer (Bio-Rad ...
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bioRxiv - Cancer Biology 2020Quote: ... The 2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one (PD98059, MEK inhibitor) and STAT3 inhibitor VI were from Calbiochem (Darmstadt, Germany). The EGF was from Millipore (Billerica ...
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bioRxiv - Developmental Biology 2024Quote: ... pro-survival cocktail (final concentrations: 100 nM B-cell lymphoma extra-large (Bcl-Xl) BH4 4-23 (197217-1MG, Calbiochem, United States), 100 μM carbobenzoxy-valyl-alanyl-aspartyl-[O-methyl]-fluoromethylketone (Z-VAD-FMK ...
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bioRxiv - Developmental Biology 2022Quote: ErbB2 signaling was inhibited by treating the embryos with 10 μM of ErbB2 inhibitor PD168393 (Calbiochem).10 µM working solution of PD168393 was prepared in 1x E3 media with 0.003% PTU ...
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bioRxiv - Biochemistry 2024Quote: ... 10 mM Imidazole (Buffer A) supplemented with 1:1000 protease inhibitors (Calbiochem Set III, Merck 539134), 3 µg/mL Benzonase-MBP ...
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bioRxiv - Cell Biology 2024Quote: ... Cell cycle analysis was performed using BrdU pulsed for 2 hr (10 µg/mL, Calbiochem #203806) and propidium iodide (PI ...
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bioRxiv - Cell Biology 2020Quote: ... Stable P2X7R-transfected clones were kept in the continuous presence of 0.2 mg/ml G418 sulphate (Geneticin, cat. n. 509290, Calbiochem, La Jolla, CA). Experiments ...
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bioRxiv - Immunology 2020Quote: ... The filtrate was collected in 1.5ml microtube followed by addition of protease inhibitors (Protease Inhibitor Cocktail SetIII, EDTA-free; Calbiochem, San Diego, CA) to protect proteins from endogenous protease activities.
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bioRxiv - Cell Biology 2020Quote: Proteasome activity was quantitatively assessed in H4 cell extracts using the β5-selective fluorogenic substrate succinyl-leucine-leucine-valine-tyrosine-4-methyl-7-courmarylamide (Suc-LLVY-AMC, Calbiochem, Burlington, MA, USA) using an adapted protocol 87 ...
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bioRxiv - Microbiology 2020Quote: ... Sumitomo Bakelite) were coated with 0.1 ml of 10 nM C1 Inh (Complement C1 Inhibitor, Human; Calbiochem) at 4°C overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were again washed with PBS pH 7.4 (3 × 10 min) and mounted in antifade (Mowiol, Calbiochem).
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bioRxiv - Cancer Biology 2024Quote: ... or ENRA-- medium (without dihydrotestosterone and ATRA) supplemented with Y-27632 (10 μM; Calbiochem, 146986-50-7)) to a final volume of 300 μL ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were washed with ice cold PBS and lysed in IP lysis buffer (50 mM Tris, pH 7.5, 10 mM NaCl, 2.5 mM MgCl2, 1 mM DTT, 0.01% digitonin [Calbiochem 300410] and protease inhibitors ...
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bioRxiv - Immunology 2023Quote: ... NF-κB (BAY11-7082; 2 μM) and RAF1 (RAF1 Kinase inhibitor I; 10 μM) were from Calbiochem. The inhibitor of EGFR (gefitinib ...
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bioRxiv - Developmental Biology 2024Quote: ... and 6 mg/mL fatty acid-free BSA supplemented with 10 µg/mL heparin sodium salt (Calbiochem). Co-incubations took place in four-well dishes as groups of 50 COCs per well at 38.5°C under an atmosphere of 5% CO2 in air and maximum humidity.
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bioRxiv - Neuroscience 2024Quote: ... Spheres were ventralized with the addition of the sonic hedgehog agonist purmorphamine (1µM, 483367-10-8, Calbiochem) for 7 days in Advanced DMEM/F12 (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... and 10 mM sodium azide in PBS supplemented with protease inhibitors (PIC, Protease Inhibitor Cocktail III, Calbiochem). Cells were then incubated on ice in fresh quenching solution 3 times for 5 min each ...
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bioRxiv - Cell Biology 2021Quote: ... NaCl-HEPES buffer with antibodies but without uEV sample as well as stained sample supplemented with 1% NP40 (Calbiochem, San Diego, CA, USA) were analyzed as controls ...
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bioRxiv - Biochemistry 2022Quote: Stable mACSF2 knockdown cells and control cells were grown on glass cover slips and treated with 40 μM all-trans-retinoic acid (Calbiochem, San Diego CA). Forty eight hours post transfection cells were fixed for 20 min with a solution of 3% formaldehyde in PBS ...
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bioRxiv - Bioengineering 2021Quote: ... Protease Inhibitor Cocktail Set I and phosphate buffered saline (PBS) tablets (140mM NaCl, 10mM phosphate buffer, 3mM KCl reconstituted in 1L of deionized water) were from Calbiochem (San Diego, CA). Collagenase 2 and collagenase 4 were from Worthington Biochemical Corporation (Lakewood ...
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bioRxiv - Developmental Biology 2022Quote: ... mature female mice (6-9 weeks C57BL/6) were injected IP with 5 IU of pregnant mare serum gonadotropin (PMSG) (Calbiochem, San Diego, CA), followed 48 hours later by 5 IU of human chorionic gonadotropin (hCG ...
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bioRxiv - Cancer Biology 2020Quote: Dissected CP specimens were dissociated using forceps under a stereoscope followed by enzymatic digestion at 37°C for 20 minutes with pronase (2 mg/ml, 537088, Calbiochem, San Diego, CA) in Hank’s balanced salt solution (HBSS ...
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bioRxiv - Biophysics 2022Quote: The activity of β-galactosidase was measured using a spectrophotometric assay in which o-Nitrophenyl-β-D-Galactopyranoside (ONPG, CAS Nr: 369-07-3 Calbiochem Sigma-Aldrich) was used as a substrate ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were solubilized for 1 h at 4□°C in buffer (50□mM HEPES-NaOH [pH 7.5], 300□mM NaCl, 2% (w/v) N-dodecyl β-d-maltoside (DDM, Calbiochem, San Diego, CA), protease inhibitor cocktail ...
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bioRxiv - Cancer Biology 2023Quote: ... Caspase-3 activity was assessed in duplicates via a fluorogenic assay using the Ac-DEVD-AMC-specific caspase-3 substrate (Calbiochem, La Jolla, CA). After substrate addition ...
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bioRxiv - Molecular Biology 2023Quote: ... the mice were anaesthetized using 2% isoflurane and given a retro-orbital injection of 21.75 mg kg−1 body weight puromycin (Calbiochem, San Diego, CA, USA) in saline to measure muscle protein synthesis73.