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Citations for Calbiochem :
301 - 350 of 445 citations for BN80933 CAS 214348 10 4 98% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: Neuro2a cells were grown on glass coverslips and incubated with 20 μM nocodazole (Calbiochem, San Diego CA). Coverslips were removed at zero time and 2 hours and were fixed for 10 min using 3% formaldehyde in PBS prior to indirect immunofluorescence analysis as described below ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The master mix recipe contained polyclonal anti-rabbit anti-MLH1 (Calbiochem, San Diego CA; diluted 1:50) or anti-rabbit anti-DMC1 (mix of DMC1) ...
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bioRxiv - Molecular Biology 2020Quote: ... PRC2 was inhibited by addition of 5μm of Ezh2 inhibitor (EI1) (CAS 1418308-27-6 Calbiochem Chemicals). For activation of NF-κB ...
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bioRxiv - Cell Biology 2023Quote: ... The lysate was then combined with 0.5 μL of Protease Inhibitor Cocktail IV (Calbiochem, San Diego, CA) and 4 μL of 25.5 U/μL benzonase nuclease (EMD Millipore ...
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bioRxiv - Microbiology 2024Quote: Parasites were treated with 50 μM of RNA Pol III inhibitor CAS 577784-91-9 (Calbiochem, Merck) after sorbitol treatment at 3 ±3hpi and RNA was harvested at 24hpi in parallel with untreated and control samples ...
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bioRxiv - Plant Biology 2024Quote: ... the soil slurries were filtered through Miracloth (22-25 µm pore size; Calbiochem, San Diego, CA, USA) and then centrifuged for 30 minutes (3000 x g) ...
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bioRxiv - Biochemistry 2024Quote: ... 1 mM phenylmethane sulfonyl fluoride (PMSF) and Protease Inhibitor Cocktail Set III (Calbiochem, San Diego, CA, USA). The cells were disrupted in an EmulsiFlex C3 homogenizer (Avestin Inc. ...
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bioRxiv - Biophysics 2022Quote: ... were mixed in 70 μL of assembly buffer (50 mM HEPES-KOH, pH 7.5, 300 mM KCl, 5 mM MgCl2, 2 mM TCEP, 4% glycerol, 2 mM ATPγS [Calbiochem]) for 5 min at 25 °C ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated overnight at 4°C in blocking solution with anti-TH (1:2,500; Chemicon) and anti-BiP (1:1,000; Calbiochem) antibodies and detected using secondary alexa-488 anti-rabbit and alexa-564 anti-mouse antibodies ...
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bioRxiv - Physiology 2023Quote: Total protein extracts were obtained from microalgal cells in exponential phase of growth (e.g. 4 days) in 50 mM Tris buffer pH 8.0 supplemented with protein inhibitor cocktail (539131, Calbiochem). Free-living and symbiotic microalgae were disrupted by bead beating with a Precellys device (Bertin Technologies ...
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bioRxiv - Cell Biology 2020Quote: ... Complete media supplemented with Rho kinase inhibitor (Calbiochem Y27632, final concentration 10 μM) was added to the cuvette ...
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bioRxiv - Cell Biology 2021Quote: Cells were treated with 10 μM and 50 μM blebbistatin (in DMSO; Calbiochem) or DMSO (vehicle control ...
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bioRxiv - Microbiology 2022Quote: ... 10 µM of indicated proteins were mixed with 100 µM nitrocefin (Calbiochem, Sigma) in a reaction buffer containing EstG storage buffer (50 mM HEPES-NaOH pH 7.2 ...
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bioRxiv - Neuroscience 2022Quote: ... or M9 containing 10 µM PMA (Phorbol-12-myristate-13-acetate, Calbiochem #524400) in a 96-well microtiter dish ...
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bioRxiv - Cell Biology 2021Quote: ... MARK/Par-1 activity inhibitor49 (MARK2i; Calbiochem 39621; 5 µM or 10 µM) was added prior to imaging ...
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bioRxiv - Molecular Biology 2020Quote: ... SMPD2 inhibitor GW4869 was used at a final concentration of 10 μM (Calbiochem). For all experiments cells were grown to 25-40% confluency states unless specified otherwise.
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bioRxiv - Developmental Biology 2023Quote: ... Enzymatic reaction was blocked by adding PBS+10% FBS+1% DNAse (Calbiochem, 260913) and cell suspension was filtered and centrifuged at 1000 rpm for 5 min ...
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bioRxiv - Synthetic Biology 2022Quote: ... 10 mM imidazole and SetIII protease inhibitor-EDTA-free (1:1000 dilution, Calbiochem) were added ...
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bioRxiv - Cell Biology 2024Quote: ... 10 µM of MG132 (diluted from 20mM stock prepared in DMSO; Calbiochem, 474790), 250 nM bafilomycin A1 (diluted from 1mM stock prepared in DMSO ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1 μl Y-27362 (Calbiochem, 688000, 10 μM, stock solution 1 mM), in a U-bottom 96-well plate (Falcon ...
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bioRxiv - Cancer Biology 2024Quote: ... the organoid expansion medium was supplemented with Y-27632 (Calbiochem, 688000, 10 μM). This medium was renewed every two days and PDOs were passaged every 7 to 14 days as described in Cartry et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... water samples from each source were collected and filtered through two layers of miracloth (Calbiochem, La Jolla, CA). One ml of the filtrate was spread aseptically onto YPS media and incubated in the dark at 24°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were infected for 1 h and an overlay of 1% carboxymethylcellulose (Aquacide II, Calbiochem, San Diego, CA) prepared in RPMI was added ...
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bioRxiv - Molecular Biology 2020Quote: ... 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reagent was obtained from Calbiochem (San Diego, CA, USA).
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bioRxiv - Physiology 2021Quote: ... and phosphatase inhibitors (2 μM microcystin-LR, and 1× Phosphatase-Inhibitor Sets I and II (Calbiochem, Hayward, CA). We centrifuged homogenates at 4,000 g for 15 minutes to remove debris ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mM EDTA and 1% Triton X-100) supplemented with protease inhibitor cocktail V (Calbiochem, San Diego, CA) and 1 mM phenylmethylsulfonyl fluoride (PMSF) ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: Brain tissue was homogenized in RIPA buffer supplemented with protease and protein phosphatase inhibitors (Calbiochem, La Jolla, CA) and subjected to SDS-PAGE followed by transferring to PVDF membranes (Millipore ...
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bioRxiv - Immunology 2021Quote: ... under the CD11c/Itgax promoter upon intraperitoneal (i.p.) injection of 4 ng/g of mouse body weight of diphtheria toxin (DT, Calbiochem) 12 hrs prior to the targeted CD11c+ cell depletion time as indicated in the relevant figure legends ...
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bioRxiv - Physiology 2021Quote: ... cells were treated with poly(I:C) (1.5 μg/mL for 4 hrs) and a combination of proteasome inhibitor (Bortezomib, 2 μM, Sigma/Calbiochem 5043140001) and prolyl endopeptidase inhibitors (Pramiracetam 10 uM ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were rinsed with cold PBS1X and then lysed in Triton-X100 buffer at 4°C (50mM HEPES pH 7.4, 150mM NaCl, 1 % Triton X-100, protease inhibitors (Calbiochem) and phosphatase inhibitor cocktails II and IV (Calbiochem)) ...
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bioRxiv - Biochemistry 2021Quote: ... cells were trypsinized and seeded for selection in complete media supplemented with 4 μg/mL puromycin (Calbiochem, Merck Millipore). All cell lines were passaged 3 times before use and kept in culture supplemented with 2 μg/mL puromycin and/or stored in liquid nitrogen.
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bioRxiv - Cell Biology 2022Quote: ... and at 48 hpi cells transduced with Wasabi-pIPFCW2 were selected with 3-4 mg/ml puromycin (Calbiochem, 540411) and sorted for mid-range expression of Wasabi.
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bioRxiv - Developmental Biology 2020Quote: ... allowed to dry for 30 min at 37 °C and mounted in MOWIOL 4-88 Reagent (Calbiochem, Darmstadt, Germany).
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bioRxiv - Cell Biology 2020Quote: ... and the phenylacetamide compound (S)-2-(4-chlorophenyl)-3,3-dimethyl-N-(5-phenylthiazol-2-yl) butanamide (Cmp58) was obtained from Calbiochem-Merck Millipore (Billerica ...
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bioRxiv - Cell Biology 2020Quote: ... The allosteric FFAR2 modulator Cmp58 ((S)-2-(4-chlorophenyl)-3,3-dimethyl-N-(5-phenylthiazol-2-yl)butanamide was obtained from Calbiochem-Merck Millipore (Billerica ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and the phenylacetamide compound (S)-2-(4-chlorophenyl)-3,3-dimethyl-N-(5-phenylthiazol-2-yl)butanamide (PA;Cmp58 [30]) was obtained from Calbiochem-Merck Millipore (Billerica ...
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bioRxiv - Microbiology 2023Quote: ... Plates were then incubated at 37 °C for 2 h with different quantities (0.125 - 4 pmol) of biotinylated PfPP1c labeled with biotin-NHS (Calbiochem) in PBS-Tween 0.1% ...
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bioRxiv - Plant Biology 2023Quote: ... The homogenate was centrifuged at 4°C for 15 min at 10,000×g and the supernatant was filtered through Miracloth (Calbiochem) to remove plant debris ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were incubated overnight at 4 °C with primary anti-RAD51 antibody (Calbiochem, PC130, 1:500 in 2% BSA), then washed five times ...
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bioRxiv - Cell Biology 2021Quote: ... and permeabilized on ice for 10 min in 25-35 ug/mL digitonin (Calbiochem) in transport buffer (TRB ...
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bioRxiv - Cell Biology 2023Quote: Translocation and mitophagy experiments were achieved by incubating cells with 10 μM Oligomycin (Calbiochem), 4 μM Antimycin A (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... diluted 10-fold in 50% MeOH containing 1 µM glycocholic acid (Calbiochem, 100.1% pure), and centrifuged for 10 min at 4000 RPM (Thermo Sorvall ST 40R ...
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bioRxiv - Developmental Biology 2024Quote: ... embryos were first manually dechorionated and then incubated in 10 µM PD168393 (Calbiochem #513033), or an equivalent volume of DMSO as vehicle control ...
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bioRxiv - Immunology 2022Quote: Cell samples or EV samples were lysed in radioimmunoprecipitation assay (RIPA) buffer supplemented with protease inhibitors (Calbiochem, CA, USA) and phosphatase inhibitors (Calbiochem) ...
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bioRxiv - Neuroscience 2020Quote: ... cells were treated with N‐[N‐(3,5‐difluorophenacetyl)‐L‐alanyl]‐sphenylglycine t‐butyl ester (DAPT) (Calbiochem, Camarillo, CA, USA) 24 h before performing the experiment ...
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bioRxiv - Physiology 2023Quote: ... Fura-2 AM and BAPTA-AM (Cat# F1221, Molecular Probes, Eugene, OR, USA and CAS# 126150-97-8, Calbiochem San Diego ...
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bioRxiv - Biochemistry 2023Quote: ... 0.2 mM DTT) supplemented with protease inhibitors (0.1 mM PMSF and 1:500 Cocktail IV (Calbiochem; San Diego, CA), and lysed by two passes through a French press at 1000 psi ...
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bioRxiv - Neuroscience 2021Quote: ... allowed to dry for 30 min at 37 °C and mounted in MOWIOL® 4-88 (Calbiochem, Darmstadt, Ger-many).
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bioRxiv - Developmental Biology 2022Quote: ... Female Hbxip+/- mice (4-6 weeks) were induced to superovulate by intraperitoneal injection of 5 IU pregnant mare serum gonadotropin (PMSG, Calbiochem) and 48 hours later 5 IU human chorionic gonadotropin (hCG ...
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bioRxiv - Immunology 2021Quote: The caspase-1 activity was determined with the fluorometric substrate Z-YVAD 7-Amido-4-trifluoromethylcoumarin (Z-YVAD-AFC, caspase-1 substrate VI, Calbiochem), as described previously (Angosto et al. ...