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Citations for Calbiochem :
501 - 522 of 522 citations for 3 Chloromethyl pyrrolidine 1 carboxylic acid benzyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... All the above stages were performed in cold phosphate-buffered saline (PBS) with 1 × Protease Inhibitor cocktail set I (Calbiochem 539,131). Eyes that were initially fixed in 10% NBF or 2.5% PFA were dissected to create eyecups ...
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bioRxiv - Immunology 2022Quote: ... at a 4:1 molar ratio of monomer:streptavidin in the presence of a 1x protease inhibitor cocktail solution (Calbiochem, Millipore Sigma). 1/5th volumes of streptavidin-PE or streptavidin-BV421 were added to each monomer every 20 minutes and incubated ...
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bioRxiv - Immunology 2023Quote: ... at a 4:1 molar ratio of monomer:streptavidin in the presence of a 1x protease inhibitor cocktail solution (Calbiochem, Millipore Sigma). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: ... cultures were lysed with a solution containing SYBR green (1:5000, Invitrogen Cat. No. S7567) (Lysis buffer: 0.16% saponin (Calbiochem/EMD #558255), 0.05 M TRIS-HCl (Roche Cat ...
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bioRxiv - Genetics 2023Quote: ... Raw values were normalized by geometric mean and used for subsequent housekeeping normalization (α-tubulin values; 1:10,000 mouse anti-tubulin; Calbiochem CP06) from the same blot.
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bioRxiv - Physiology 2023Quote: ... MLEC were incubated with appropriate fluorophore-labelled secondary antibodies for 1 hour and mounted with Mowiol (Calbiochem, San Diego, CA, USA). Nuclei were visualized by DAPI staining ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.1 mM EGTA, 1% Triton X-100, 0.1% sodium deoxycholate, 0.5% SLS, 0.1% SDS, 0.5 mM PMSF, Calbiochem Complete Protease Inhibitor Cocktail V). The suspension was incubated for 30 min at 4°C and then sonicated with Covaris ME220 focused-ultrasonicator (50 alternating 15-s ON and 45-s OFF intervals ...
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bioRxiv - Bioengineering 2020Quote: ... Tested additives were added to the qRT-PCR reaction mix at the following concentrations: 1% w/v Tween-20 (Calbiochem 655204-100mL), 0.8 µg/µL BSA (Fisher BP675-1) ...
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Myosin VI regulates ciliogenesis by promoting the turnover of the centrosomal/satellite protein OFD1bioRxiv - Cell Biology 2021Quote: ... pH 7.5, 250 mM NaCl, 1 mM EDTA, 5% Glycerol, 0.1% NP40, Protease Inhibitor Cocktail set III, Calbiochem, and 0.1 mM PMSF). Sonicated lysates were cleared by centrifugation at 16,000 rpm for 30 minutes ...
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bioRxiv - Physiology 2023Quote: ... Normal goat serum was used as a blocking agent and slides were incubated in biotinylated hyaluronan binding protein (HABP, 1:500, CalBiochem, Burlington, MA) for one hour ...
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bioRxiv - Genetics 2024Quote: ... and re-suspended in 300 μL of culture media containing 1 μL of FITC-VAD-FMK (Calbiochem Caspase Detection Kit, cat# QIA90) and incubated for 45 minutes in a 37°C incubator with 5 % CO2 ...
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bioRxiv - Neuroscience 2024Quote: ... Immunostained sections were counterstained with 4’,6-diamidino-2-phenylindole (DAPI, 1:1000, KPL, Gaithersburg, Maryland USA) and mounted with MOWIOL 4-88 (Calbiochem, Lajolla,CA).
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bioRxiv - Neuroscience 2021Quote: ... cells were rinsed two times with pre-warmed medium and incubated for 30 min at 37°C with rabbit anti-GluA1 (1/150; Calbiochem/Millipore, RRID: AB_564636) in culture medium ...
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bioRxiv - Cell Biology 2021Quote: ... NaCl-HEPES buffer with antibodies but without uEV sample as well as stained sample supplemented with 1% NP40 (Calbiochem, San Diego, CA, USA) were analyzed as controls ...
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bioRxiv - Cancer Biology 2023Quote: ... hatched zebrafish embryos were engrafted via the Duct of Cuvier with 1×105 cells/µL tumor cells resuspended in 2% polyvinypyrrolidone 40 (PVP-40, Calbiochem, San Diego, USA) using borosilicate glass capillary needles (1mm O.D ...
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bioRxiv - Cell Biology 2023Quote: ... RPE-1 cells transfected with either siR-NAs targeting Luciferase or MiniBAR for 72 h were treated with Y27632 (Calbiochem 146986-50-7) at 50 µM for 24 h in DMEM-F12 without SVF before fixation.
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bioRxiv - Molecular Biology 2023Quote: ... the mice were anaesthetized using 2% isoflurane and given a retro-orbital injection of 21.75 mg kg−1 body weight puromycin (Calbiochem, San Diego, CA, USA) in saline to measure muscle protein synthesis73.
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bioRxiv - Biophysics 2024Quote: ... Native thick filaments were then released from the myofibrils by enzymatic digestion with the addition of Calpain-1 from porcine erythrocytes (CALBIOCHEM, 0.1U/μl, final concentration) and Calcium Acetate (2mM final concentration ...
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bioRxiv - Neuroscience 2020Quote: ... Bacteria were resuspended in (50mM HEPES, 250mM NaCl, 10% glycerol plus 1% Triton X-100, supplemented with protease inhibitors (Merck, Calbiochem set III, Cat. No. 539134) and subsequently sonicated ...
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bioRxiv - Neuroscience 2020Quote: ... Cell lysis of all samples was performed after two washes with ice-cold PBS by incubating cells for 20 min in ice-cold 1% Triton X100 in PBS with protease inhibitor cocktails (Merck, Calbiochem set III, Cat. No. 539134), subsequent thorough scraping and sonication.
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bioRxiv - Pathology 2022Quote: ... and a mouse monoclonal antibody raised against Simian Virus-40 (SV-40, Macaca mulatta polyomavirus-1, Betapolyomavirus macacae) LT antigen (CalBiochem®, Clone PAb416, Sigma-Aldrich) was used as primary antibody at a 1:200 dilution ...
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bioRxiv - Cell Biology 2022Quote: ... or kinase domain ERK3 in kinase buffer (250 mM HEPES pH 7.5, 500 mM NaCl, 25 mM MgCl2, supplemented with protease inhibitor cocktail Set I-Calbiochem 1:100 (Cat# 539131, Merck Millipore) and phosphatase inhibitors ...