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Citations for Calbiochem :
451 - 500 of 522 citations for 3 Chloromethyl pyrrolidine 1 carboxylic acid benzyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Cells were grown at 37°C to OD = 0.8 and expression was induced with 0.3 mM IPTG (Isopropyl b-D-1-thiogalactopyranoside, Calbiochem) overnight at 18°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were incubated for 1 h with the ADAM17 inhibitor Batimastat (BB-94) at 10 μM (196440, Calbiochem), with the selective ADAM10 inhibitor GI254023X (GI ...
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bioRxiv - Molecular Biology 2024Quote: ... The pellet was suspended in 2 ml of lysis/binding buffer (25 mM HEPES/KOH, pH 7.6, 10 % glycerol, 150 mM KCl, 1 mM dithiothreitol, 0.1 % Elugent (Calbiochem), loaded into SHREDDER tubes (Pressure Biosciences Inc ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... sections were incubated with rabbit anti-c-Fos antibody diluted with the blocking solution (1:20000, #PC38, Calbiochem) for 2 days at 4°C ...
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bioRxiv - Pathology 2024Quote: ... 2% DDM: 10µl/mg flies) supplemented with 1:1000 (v/v) Protease Inhibitor Cocktail Set III (Calbiochem, USA). Rat anti-HRP-conjugated-HA (3F10 ...
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bioRxiv - Biophysics 2024Quote: ... Cell pellet was resuspended then resuspended in phosphate-buffer saline (PBS) containing 1 mM phenylmethylsulfonyl fluoride (PMSF) (Calbiochem), 50 μg/mL DNase I (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2021Quote: ... and 2 mM glutamine for 24 h prior to treatment with either TGF-β (1 ng/mL, Millipore Calbiochem) or 10 uM TGF-β inhibitors (SB431542 ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were rinsed with cold PBS1X and then lysed in Triton-X100 buffer at 4°C (50mM HEPES pH 7.4, 150mM NaCl, 1 % Triton X-100, protease inhibitors (Calbiochem) and phosphatase inhibitor cocktails II and IV (Calbiochem)) ...
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bioRxiv - Cell Biology 2021Quote: ... 50 μg of the total RNA was incubated for 1 h at 4 °C with 10 μl of the anti-2,2,7-trimethylguanosine-antibody (Calbiochem Milipore) coupled to sepharose beads (Fig ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then harvested using lysis buffer (50mM HEPES pH7.4, 150mM NaCl, 1mM MgCl2, 1mM NaF, 1% Triton-x-100, protease inhibitor cocktail I [Calbiochem) and centrifuged at 14,000rpm for 10 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... collected and lysed in lysis buffer RGMT (50mM HEPES pH 7.4, 150mM NaCl, 1mM MgCl2, 1mM NaF, 1% Triton-x-100, plus protease inhibitor cocktail I [Calbiochem]). Cellular lysates were cleared by centrifugation and 225µL of total protein extract was incubated with biotin-tagged CIGB-300 (100μM ...
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bioRxiv - Molecular Biology 2022Quote: ... The cell pellet was lysed at 0.5 × 109 cells/ml in ice-cold lysis buffer (0.1 mM TLCK, 1 μg/ml Leupeptin, 1x Phosphatase Inhibitor Cocktail II tablet (Calbiochem), 1 mM PMSF ...
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bioRxiv - Developmental Biology 2022Quote: ... Tissues were homogenized in cold lysis buffer (0.1% NP-40, 50mM Tris-HCl [pH 7.2], 250mM NaCl, 2mM EDTA, phosphatase inhibitor mixture I and II [Calbiochem] and one tablet of Minicomplete [Roche] per 10ml ...
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bioRxiv - Biochemistry 2023Quote: ... Culture was then cooled to 16℃ and induced with 0.5 mM dioxane-free isopropyl B-D-1-thiogalactopyranoside (IPTG) (Calbiochem) overnight at 16℃ ...
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bioRxiv - Biochemistry 2023Quote: ... Ubiquitin was expressed as described above except that the 2 L culture was induced with 0.4 mM dioxane-free isopropyl B-D-1-thiogalactopyranoside (IPTG) (Calbiochem) for four hrs at 37℃ ...
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bioRxiv - Biochemistry 2023Quote: ... Culture was then cooled to 16℃ and induced with 0.4 mM dioxane-free isopropyl B-D-1-thiogalactopyranoside (IPTG) (Calbiochem) overnight at 16℃ ...
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bioRxiv - Biochemistry 2023Quote: ... Culture was then cooled to 16℃ and induced with 0.4 mM dioxane-free isopropyl B-D-1-thiogalactopyranoside (IPTG) (Calbiochem) overnight at 16℃ ...
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bioRxiv - Molecular Biology 2022Quote: ... pellets were resuspended in 800 μl of ice-cold wash buffer (50 mM NaH2PO4, 300 mM NaCl, 0.05% Tween, 1:200 diluted protease inhibitor cocktail set III (Calbiochem)) supplemented with 0.1 U/μL of recombinant RNase inhibitor (wash buffer-RIn ...
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bioRxiv - Neuroscience 2024Quote: ... 1 mM Na3VO4 and 500 nM Cantharidin (#3322.1 Roth)] and commercial protease inhibitors (protease inhibitor cocktail III, #539134, Calbiochem). Phosphatase inhibitors were omitted when Lambda phosphatase treatment was required ...
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bioRxiv - Molecular Biology 2023Quote: Whole-cell protein extracts were prepared using hot lysis buffer (1%SDS, 5mM EDTA, 50mM Tris, pH7.5) supplemented with protease inhibitor mixture (Calbiochem). Protein samples were diluted and boiled in 5X protein loading buffer (10% SDS ...
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bioRxiv - Cell Biology 2022Quote: ... alone bound to glutathione sepharose 4B beads in 0.7 ml of binding buffer (1X PBS, 150 mM NaCl, 1% Triton X-100, Calbiochem EDTA-free protease inhibitor cocktail set III ...
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bioRxiv - Immunology 2022Quote: ... and 1 × 106 cells were treated with rapamycin (10 ng/ml, 30 min, 37°C; Calbiochem, San Diego, CA) prior to treatment with rhGH.
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bioRxiv - Cancer Biology 2022Quote: ... Boston Bioproducts (Cat# BP-115X) supplemented with protease inhibitor cocktail at a dilution of 1: 100 (Calbiochem, Cat#539131) and phosphatase inhibitors at a dilution of 1 ...
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bioRxiv - Biochemistry 2023Quote: ... 0.2 mM DTT) supplemented with protease inhibitors (0.1 mM PMSF and 1:500 Cocktail IV (Calbiochem; San Diego, CA), and lysed by two passes through a French press at 1000 psi ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were incubated overnight at 4 °C with primary anti-RAD51 antibody (Calbiochem, PC130, 1:500 in 2% BSA), then washed five times ...
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bioRxiv - Neuroscience 2024Quote: ... The array was washed three times for 5 min with TBST and incubated for 1 hr at room temperature with secondary antibody horseradish peroxidase-conjugated anti-rabbit or anti-mouse secondary antibodies (1:10,000, Calbiochem) diluted in TBST ...
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bioRxiv - Immunology 2021Quote: ... GMDCs were incubated for 2 h at 37°C with 50 µM MARK inhibitor (MARK/Par-1 Activity Inhibitor, 39621; Calbiochem), 50 nM SIK inhibitor (HG-9-91-01 ...
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bioRxiv - Immunology 2021Quote: The caspase-1 activity was determined with the fluorometric substrate Z-YVAD 7-Amido-4-trifluoromethylcoumarin (Z-YVAD-AFC, caspase-1 substrate VI, Calbiochem), as described previously (Angosto et al. ...
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bioRxiv - Cell Biology 2022Quote: ... and AR-C118925 {5-[[5-(2,8-dimethyl-5H-dibenzo[a,d]cyclohepten-5-yl)-3,4-dihydro-2-oxo-4-thioxo-1(2H)-pyrimidinyl]methyl]-N-2H-tetrazol-5-yl-2-furancarboxamide} were obtained from Calbiochem-Merck Millipore (Billerica ...
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bioRxiv - Cancer Biology 2022Quote: ... Whole cell samples were ground in liquid nitrogen and lysed in 8 M urea supplemented with 1% protease inhibitor cocktail (Calbiochem). Lysates were centrifuged at 12,000 x g and the supernatant was quantified by BCA assays (Beyotime) ...
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bioRxiv - Microbiology 2022Quote: ... The furin inhibitor decanoyl-RVKR-cmk and mannosidase-1 inhibitor deoxymannojirmycin (DMJ) were purchased from Calbiochem (San Diego, California, USA).
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bioRxiv - Immunology 2022Quote: The caspase-1 activity was determined with the fluorometric substrate Z-YVAD 7-Amido-4-trifluoromethylcoumarin (Z-YVAD-AFC, caspase-1 substrate VI, Calbiochem), as described previously (Angosto et al. ...
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bioRxiv - Cancer Biology 2020Quote: ... slides were counterstained with DAPI (1:50000 from 10 mg/ml stock solution, Serva Eletrophoresis) and embedded with geltol (Calbiochem). 2 representative pictures of 4 biological replicates per genotype were taken with LSM 5 Exciter (Zeiss ...
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bioRxiv - Genetics 2020Quote: Drosophila larval brains were homogenized with pestles in RIPA buffer plus the addition of proteinase inhibitors 1:200 (539134, Calbiochem). The homogenate was centrifuged at 13000 rpm for 20 minutes at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: Mammalian cells were lysed using RIPA buffer (50mM Tris, pH 7.4, 150mM NaCl, 1%NP-40, 0.5%sodium deoxycholate) with protease inhibitors (Calbiochem, Billerica, MA). Protein samples were separated on 4–15%gradient TGX precast gels (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... the cell pellet was lysed by sonication in lysis buffer (2mM Tris–HCl pH 7.4, 0.5M NaCl, 20mM imidazole, 10% glycerol, Protease Inhibitor Cocktail Set III Calbiochem, 1:10,000) and centrifuged (10,000g ...
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bioRxiv - Biochemistry 2022Quote: ... Nuclei were stained with DAPI (1:1000 in PBS, AppliChem, Darmstadt, Germany) and coverslips were mounted on Mowiol 4-88 (Calbiochem, Merck Millipore ...
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bioRxiv - Neuroscience 2023Quote: ... mg of tissue/µl of NP-40 lysis buffer (20mM Tris- HCl pH 7.4, 150 mM NaCl, 100mM EDTA pH 7.4, 1% NP-40; Calbiochem Cat#492016) with protease inhibitors (cOmplete™ ...
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bioRxiv - Neuroscience 2022Quote: Supernatant was removed and beads were resuspended in 250μl of antibody buffer (wash buffer + 0.05% Digitonin (Calbiochem #11024-24-1) + 2mM EDTA ...
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bioRxiv - Biochemistry 2022Quote: ... lysed in M-PER mammalian protein extraction reagent (Thermo-Fisher) with 1/1000 of the EDTA-free protease inhibitor cocktail Set III (Calbiochem), and centrifuged for 30 min at 15,000 × g at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Cells were left unstimulated or stimulated for indicated time at 37 °C with PMA (10 ng/ml; Alexis) and ionomycin (1 μM; Calbiochem) or solvent (DMSO) ...
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bioRxiv - Immunology 2022Quote: Caspase-1 activity was determined with the fluorometric substrate Z-YVAD 7-Amido-4-trifluoromethylcoumarin (Z-YVAD-AFC, caspase-1 substrate VI, Calbiochem) as described previously [47,48] ...
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bioRxiv - Cell Biology 2023Quote: ... and cells were held for 16 hours in 1% Lipoprotein Deficient Serum (LDS) (Biomedical Technologies, BT907) and 25 μg/ml ALLN (Calbiochem) for 30 min prior to harvesting.
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bioRxiv - Cell Biology 2024Quote: ... 1% Triton X-100) supplemented with protease and phosphatase inhibitors (Protease inhibitor set III and Phosphatase inhibitor set II, Calbiochem), incubated on ice for 30 min ...
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Autophagy suppression in DNA damaged cells occurs through a newly identified p53-proteasome-LC3 axisbioRxiv - Cell Biology 2024Quote: ... monolayers of HCT116 cells were incubated at 37 °C and under 5% CO2 in the presence of Bleocin (0.1, 1, 10, 100 μg/mL, 5% H2O as the carrier control; #203408, Calbiochem, USA), Etoposide (5 μg/mL ...
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bioRxiv - Cell Biology 2024Quote: ... Adh1 was probed with rabbit polyclonal primary antibody which was a gift from Claudio De Virgilio’s lab (Université de Fribourg, Switzerland) used with a dilution of 1:50,000 (Calbiochem ref 126745). The secondary antibody was Goat-anti-rabbit-HRP conjugate used with a dilution of 1:5000.
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bioRxiv - Immunology 2024Quote: ... Fexp are target+CTL samples and Flyse is the maximal target lysis in the presence of 1% Triton X-100 (Calbiochem). The γ value was measured at time zero ...
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bioRxiv - Microbiology 2024Quote: ... Viability was assessed after staining cells with 1 µg/mL propidium iodide (PI, stored at 4°C protected from light; Calbiochem). Cell number and percentage were obtained from gatings done on the Attune NxT software.
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bioRxiv - Cell Biology 2021Quote: Commercially available antibodies against the following proteins were used for Western blotting in the indicated dilutions: BRCA2 (OP95, Calbiochem, 1:1000), RAD51 (70-001 ...
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bioRxiv - Neuroscience 2022Quote: ... the slices were incubated overnight in a mixture of primary antibodies anti-FOS (rabbit anti-FOS, 1:10000, cat: p38, Calbiochem, USA) and anti-GAD67 (mouse anti-GAD67 ...