Labshake search
Citations for Leica :
601 - 635 of 635 citations for Lamin A C Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Decoupling cell size homeostasis in diatoms from the geometrical constraints of the silica cell-wallbioRxiv - Microbiology 2023Quote: ... the discs were loaded into a Leica ICE high pressure freezing machine and vitrified at liquid nitrogen temperature (−192 °C) at 210 MPa (Leica Microsystems GmbH, Wetzlar, Germany). Vitrified samples were kept in liquid nitrogen until freeze-substitution in a freeze-substitution device at −90 °C (EM ASF2 ...
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Decoupling cell size homeostasis in diatoms from the geometrical constraints of the silica cell-wallbioRxiv - Microbiology 2023Quote: ... Epon-embedded samples were cured at 70 °C for 72 and then sectioned to 70 nm thin sections using Ultracut UCT ultra-microtome (Leica Microsystems GmbH, Wetzlar, Germany) equipped with an Ultra 45° diamond knife (Diatome Ltd ...
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bioRxiv - Cancer Biology 2023Quote: 4 µm FFPE tissue sections were baked for 1 hour at 62°C in vertical slide orientation with subsequent prestaining of deparaffinization (Leica, Bond Dewax Solution, AR9222) and 35 minutes of Bond ER2 (Leica ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was incubated for 30 s at 90 % relative humidity and 16°C inside Leica EM ACE 600 (Leica EM GP2 plunge freezer), blotted for 1.2 s and vitrified in liquid ethane at liquid nitrogen temperature ...
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bioRxiv - Cell Biology 2022Quote: ... or for 4–6 s at 4°C and ~ 86% and plunge frozen using a Leica EM GP (Leica Microsystems Inc, Buffalo Grove, IL). The samples were imaged in an FEI Titan Krios electron microscope operated at 300 kV with a K2 Summit camera and energy filter (Gatan ...
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bioRxiv - Biochemistry 2023Quote: ... the grids were flash-frozen in liquid ethane at ∼90 % humidity and 4 °C with a Leica EM GP rapid-plunging device (Leica, Buffalo Grove, IL, USA) before being transferred into liquid nitrogen for storage ...
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bioRxiv - Biochemistry 2023Quote: ... Rat hippocampus tissue punches and thin sections were prepared according to published protocols.33 Rat brain hemisphere slices 16 µm thick were cut at -16°C using a cryostat (Leica CM3050 S, Leica Biosystems GmbH, Wetzlar, Germany). Brain slices containing hippocampal areas of all three animals were deposited on the same slide ...
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bioRxiv - Cell Biology 2023Quote: ... for 4 hours and followed by Lowicryl HM20 for 24 hours with agitation and polymerized under UV exposure at −45 °C in an automated processor (AFS2, Leica Microsystem Inc., Vienna, Austria). Ultrathin sections (90 nm thickness ...
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bioRxiv - Developmental Biology 2024Quote: ... Sections were mounted with Fluorsave (Table 1S) and stored in the dark at 4°C until imaging on a Leica Stellaris5 confocal microscope (Leica Microsystems CMS GmbH, Germany). Quantification was performed as previously described42 ...
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bioRxiv - Cell Biology 2024Quote: ... Tissues were cast into molds together with liquid paraffin and cooled to form a solid paraffin block with embedded tissue (Leica EG1150 H Paraffin Embedding Station and Leica EG1150 C Cold Plate). Paraffin-embedded WAT was sliced into 5-μm sections using a ThermoScientific HM 355S Automatic Microtome and mounted on Surgipath X-tra Microscope Slides (Leica Biosystems).
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bioRxiv - Cancer Biology 2021Quote: ... and the following primary and secondary antibodies were used: rabbit anti-p53 (Leica Biosystems, NCL-L-p53-CM5p, 1:5000), rabbit anti-Mettl3 (Abclonal ...
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bioRxiv - Cancer Biology 2022Quote: ... Slides were washed with TBST and incubated with PowerVision Poly-HRP anti-rabbit IgG or anti-mouse IgG (Leica Biosystems) in a humidified chamber at 37°C for 30 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... for 8 minutes at room temperature (Poly-HRP-mouse anti-rabbit IgG, BOND Polymer Refine Detection System, Leica, Germany; DS9800). A color reaction with 3,3-di-amino-benzidine (DAB chromogen ...
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bioRxiv - Cancer Biology 2021Quote: ... The slides were incubated with a secondary antibody (rabbit anti-mouse IgG, BOND Polymer Refine Detection System, Leica, Germany, DS9800) for 8 minutes at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... The brains were transferred and kept in 30% sucrose in PBS for more than 48 h at 4 °C and then sectioned using a freezing microtome (SM2010R, Leica; EF-13, Nihon Microtome Laboratory) at a thickness of 40 μm parallel to the coronal plane ...
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bioRxiv - Cell Biology 2020Quote: Cells seeded at low density on glass bottom Willco dishes or cell-collagen cultures were monitored by temperature- and CO2-controlled bright-field inverse microscopy (37°C, 5%; air objectives, 10×, NA 0.20, Leica; 20×, NA 0.30, Leica; and 10×, NA 0.25, Nikon DiaPhot 300). Images were collected by CCD cameras (Sentech or Hamamatsu C8484-05G) ...
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bioRxiv - Biophysics 2020Quote: ... the grids were lifted and held by tweezers inside the plunge freezer chamber at 90% relative humidity and 22 °C (Leica EM-GP plunger, Leica Microsystems). 1 μL drop of X5 concentrated gold nanoparticle (150 nm ...
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bioRxiv - Bioengineering 2020Quote: ... and polymerized at 60 °C for 48 h.[73] Ultrathin sections of ∼70 nm were produced in an ultra-microtome (Leica Biosystems, Buffalo Grove, IL, USA), transferred to copper grids (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... The grid is immediately plunged into liquid ethane at its freezing point (−183 °C) using an automatic plunger (Leica, EM GP, Buffalo Grove, IL, USA). The vitrified specimens are analyzed using a FEI Tecnai 12 G2 TEM at 120 kV with a Gatan cryo-holder maintained at −180 °C ...
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bioRxiv - Neuroscience 2021Quote: ... The fly was positioned upside down with its head and part of the thorax on top of the glue layer under a dissection microscope (Leica M205 C, Planapo 10x objective) with forceps ...
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bioRxiv - Cell Biology 2022Quote: Plates seeded with NRVMs were incubated in a stagetop incubator at 37°C with 5% CO2 (TokaiHit, Shizuoka, Japan) on a DMi8 wide-field fluorescence microscope (Leica Microsystems, Inc., Buffalo Grove, IL). Spontaneous contractions were recorded real-time under phase contrast microscopy ...
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bioRxiv - Molecular Biology 2021Quote: ... primary striatal neurons were stained with 100 nM MitoSOX™ Red reagent for 30 min at 37 °C and examined by the Automated Inverted Microscope DMI4000B (Leica Camera AG, Wetzlar, Germany).
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Oxytocin receptor activation does not mediate associative fear deficits in a Williams Syndrome modelbioRxiv - Animal Behavior and Cognition 2021Quote: ... Brains were frozen on crushed dry ice and then stored at −80 °C until sliced into 20 μm coronal sections in a cryostat (Leica Biosystems 1850, Buffalo Grove, IL) at −16 to −18 °C ...
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bioRxiv - Pathology 2022Quote: ... and incubated with a 1:200 dilution of anti-4-HNE and anti-AKR1A1 primary antibody overnight at 4°C primary antibody along with the Novolink™ Max Polymer Detection System (Leica Biosystems, Wetzlar, Hesse, Germany) and DAB substrate (3,30-diaminobenzidine ...
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bioRxiv - Neuroscience 2022Quote: ... or LNPK KO hSO assembled with unlabeled hCO) were imaged under environmentally controlled conditions (37°C, 5% CO2) on a confocal microscope (Leica Stellaris, 20x objective, 2x zoom). The samples were placed in the controlled environment for 1 hour before imaging ...
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bioRxiv - Molecular Biology 2022Quote: Muscle samples that were collected and preserved in OCT as described above were sectioned at a thickness of 10 μm in a cooled (−22°C) cryostat (Leica Biosystems; Buffalo Grove, IL, USA) and electrostatically adhered to positively charged histology slides ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We sectioned brains on a coronal plane at 150 μm using a cryostat at -20°C (Leica CM3050 S, Leica Biosystems Inc., Buffalo Grove, IL) and stored the sections at -70°C until we dissected the medial prefrontal cortex ...
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bioRxiv - Cell Biology 2023Quote: ... in complete DMEM at 37°C and treated with 45 minutes of DMSO or 1mM LLOMe before immediate cryo-fixation by high pressure freezing (Leica EM ICE High Pressure Freezer), with 20% BSA in DMEM as a cryoprotectant ...
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bioRxiv - Microbiology 2023Quote: ... tissue sections were incubated with the primary antibody (rabbit polyclonal anti-Influenza A virus NP [ThermoFisher Scientific, PA5-32242] diluted 1:500 in Primary Antibody diluent [Leica Biosystems]) for 30 min at ambient temperature followed by a polymer-labeled goat anti-rabbit IgG coupled with alkaline phosphatase (30 min) ...
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bioRxiv - Cancer Biology 2023Quote: ... Goat anti-rabbit-HRP was used as the secondary antibody with the BOND polymer refine detection system (Leica, catalog no. DS9800). Images were taken by Zeiss LSM710 confocal microscope with a 10X ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were then moved to 4°C for 5 min and immediately imaged using a confocal microscope (Leica SPX5 with a 63X 1.4 N.A. oil objective). Internalization of antigen was quantified by determining the percentages of cells with intracellularly-located AF488-Fab’ goat-anti-mouse IgM+G puncta in each field and by measuring the AF488 FI associated with intracellular puncta in individual cells ...
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bioRxiv - Physiology 2022Quote: ... Slides were washed and incubated with anti-rabbit poly-HRP-IgG secondary antibody (Novolink Polymer Detection System, Leica Biosystems, cat# RE7200-CE) for 30 minutes at room temperature ...
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bioRxiv - Bioengineering 2022Quote: ... and stained using immunohistochemistry (IHC) for collagen I (1:4000, polyclonal rabbit, lifespan biosciences, Seattle WA) via a Bond-Max autostaining system (Leica biosystems, Lincolnshire, IL). Stained kidneys were scanned at high resolution using a CRi Panoramic SCAN 40x Whole Slide Scanner (Perkin-Elmer).
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bioRxiv - Immunology 2022Quote: ... and stained using immunohistochemistry for collagen I (1:4000, polyclonal rabbit, lifespan biosciences, Seattle WA) via a Bond-Max autostaining system (Leica biosystems, Lincolnshire, IL). Stained kidneys were scanned at high resolution using a CRi Panoramic SCAN 40x Whole Slide Scanner (Perkin-Elmer).
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bioRxiv - Immunology 2022Quote: ... and stained using immunohistochemistry (IHC) for collagen I (1:4000, polyclonal rabbit, lifespan biosciences, Seattle WA) via a Bond-Max autostaining system (Leica biosystems, Lincolnshire, IL). Stained kidneys were scanned at high resolution using a CRi Panoramic SCAN 40x Whole Slide Scanner (Perkin-Elmer).