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Citations for Leica :
551 - 600 of 635 citations for Lamin A C Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... The organs were then embedded into paraffin wax using a HistoCore Arcadia C and H paraffin embedding station (Leica Biosystems, Buffalo Grove, IL). Each organ was sliced into 4 µm sheets using a RM2235 manual microtome (Leica Biosystems ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were dissected and sectioned into 40 μm-thick coronal slices at −20°C in a cryostat (Leica CM1850, Leica Biosystems, IL, USA). Coronal slices were then thaw-mounted on glass slides ...
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bioRxiv - Neuroscience 2020Quote: ... Motoneurons were patched at room temperature (approx 21°C) using infrared-differential interference contrast (IR-DIC) optics on a DMLFSA microscope (Leica DMLFSA; Leica Microsystems). All lumbar motoneurons were selected from the lateral motor nuclei of segments L1-6 and all cervical cells were selected from the lateral motor nuclei of slices made from C4-8 ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were dissected and sectioned into 40 μm-thick coronal slices at −20°C in a cryostat (Leica CM1850, Leica Biosystems, IL, USA). Coronal slices were then thaw-mounted on glass slides ...
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bioRxiv - Cell Biology 2021Quote: ... Brains were then cryoprotected by submersion in 30% sucrose in PBS at 4°C for 24h and sliced into 20-30 μm coronal sections using a Leica CM1950 cryostat (Leica Microsystems, Wetzlar, Germany). The sections were stored in PBS containing 0.01% sodium azide at 4°C until immunostaining ...
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bioRxiv - Biochemistry 2020Quote: ... The grids were blotted for 4 seconds and vitrified by rapidly plunging into liquid ethane at −182 °C using Leica EM GP plunger (Leica Microsystems, Vienna, Austria). The frozen grids were stored in liquid nitrogen until the day of cryo-EM data collection.
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bioRxiv - Microbiology 2021Quote: ... the excess of solution was manually blotted using a Whatman filter paper and then the grid was plunge-frozen into liquid ethane cooled down at - 174°C by liquid nitrogen in a Leica EM CPC cryo-plunger (Leica Microsystems, Wetzlar, Germany). Three grids were prepared per condition ...
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bioRxiv - Microbiology 2020Quote: ... While keeping the orientation succession the slices were further processed into paraffin blocks using the Leica ASP6025 tissue processor and Leica EG1150H+C tissue embedding centre (both Leica Biosystems, Nussloch, Germany). Three serial adjacent 2.5 μm thick sections were then cut with the Leica RM 2255 Microtome (Leica Biosystems ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were removed from freshly euthanized mice and placed in 10% formalin at 4 °C for 24 h before embedding in paraffin blocks and preparing 4-μm tissue sections using a microtome (Leica Microsystems, Wetzlar, Germany). We detected MAGL mRNA in situ using the RNAscope 2.5 Assay for Ventana Discovery Ultra system (ACD Bio ...
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bioRxiv - Biochemistry 2022Quote: ... The grids were blotted for 4 seconds and vitrified by rapidly plunging into liquid ethane at −182 °C using Leica EM GP plunger (Leica Microsystems, Vienna, Austria). The frozen grids were stored in liquid nitrogen until the day of cryo-EM data collection.
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bioRxiv - Pathology 2022Quote: ... Heat-induced epitope retrieval (HIER) was conducted by heating slides to 95°C for 15 minutes in EDTA-based ER2 buffer (Leica Biosystems, Richmond, IL). Slides were treated in protease (Advanced Cell Diagnostics ...
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bioRxiv - Neuroscience 2022Quote: ... Cryoprotected spinal cords were embedded in optimal cutting temperature (OCT) compound and frozen at −80 °C until cryostat sectioning (Leica Lm 1850, Germany). Transverse lumbar spinal cord cryosections were collected in serial at 30-μm or 40-μm thickness ...
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bioRxiv - Neuroscience 2020Quote: ... brains were removed from the skull and 300 μm-thick sagittal slices containing the striatum were cut in ACSF at 4°C using a VT1000S vibratome (Leica Microsystems, Wetzlar, Germany). Slices were then kept in a chamber containing ACSF at 31.5°C for 15 minutes and slowly cooled down and maintained at 26.5°C ...
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bioRxiv - Pathology 2021Quote: ... Heat-induced epitope retrieval was conducted by heating slides to 95°C for 20 minutes in sodium citrate-based ER1 buffer (Leica Biosystems, Richmond, IL) before immunostaining ...
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bioRxiv - Pathology 2021Quote: ... Heat-induced epitope retrieval was conducted by heating slides to 95°C for 15 minutes in EDTA-based ER2 buffer (Leica Biosystems, Richmond, IL). The SARS-CoV-2 probe (cat ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were extracted and snap-frozen on dry ice and sectioned at -20°C in 15 µm-thick coronal slices using a cryostat (Leica CM 1860 UV) and mounted onto Colorfrost Plus glass slides (Fisher 1255017) ...
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bioRxiv - Microbiology 2021Quote: ... Polymerization of these epoxy blocks was performed at 70 °C and then they were sectioned with an untramicrotome (Leica microsistems Gmbh, Wein Austria). Very small small sections of 80 nm thickness were collected on a cupper grid of 200 mesh (pore size ...
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bioRxiv - Neuroscience 2019Quote: ... brains were removed from the skull and 300μm-thick parasagittal slices (horizontal for thalamic stimulation experiments) were cut in ACSF at 4°C using a VT1000S vibratome (Leica Microsystems, Wetzlar, Germany). Individual slices were then submerged in a recording chamber mounted on the stage of an upright BX51WI microscope (Olympus ...
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bioRxiv - Neuroscience 2019Quote: ... the neurons at the end of treatment were stained with JC-1probe solution at 37 °C for 20 min and observed under fluorescence microscope (DMi8, Leica Biosystems, Wetzlar, Germany). The mitochondria were also extracted by a mitochondrial extraction kit ...
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bioRxiv - Plant Biology 2019Quote: ... The abaxial side of the leaf samples was imaged using a 63×/1.2 water immersion objective lens (Leica C-APOCHROMAT 63×/1.2 water). FLIM experiments were performed using a Leica TCS SP8X confocal microscope equipped with TCSPC (time correlated single photon counting ...
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bioRxiv - Physiology 2019Quote: ... 5 mm of the mid-belly of lateral gastrocnemius muscle was cut into 10-μm thick frozen muscle cross-sections at −20°C with a cryostat (Leica, Wetzlar, CM1850, Germany). Because of the uneven thickness of the diaphragm ...
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bioRxiv - Cell Biology 2021Quote: The epithelium at ALI were incubated at 37°C and 5% CO2 in the 3i Marianis/Yokogawa Spinning Disk Confocal Microscope (Leica Microsystems, TX, USA) and high speed time-lapse videos were taken at 32X air at 100 Hz with a total of 250 frames using a scientific Hamamatsu C1140-42U30 CMOS camera (Hamamatsu Photonics ...
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bioRxiv - Developmental Biology 2020Quote: Imaging of embryos and tissues was performed using a Leica MZ 16F dissecting microscope equipped with a Leica DFC420 C digital camera supported by the Leica Application Suite software package (LAS, version 3 or 4; Leica Microsystems, Germany/Switzerland), and Leica EL6000 fluorescence light source.
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bioRxiv - Cell Biology 2022Quote: ... Vials were loosely capped and transferred to the chamber of the freeze substitution machine that contained about ½ inch-deep bath of ethanol to improve thermal conductivity and was pre-cooled to -112°C (EM AFSII, Leica Microsystems, Wetzlar, Germany). Within about 10 minutes in the freeze substitution machine ...
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bioRxiv - Immunology 2022Quote: Cryo-fluorescence microscopy samples were transferred to the cryo-FIB-SEM microscope in liquid nitrogen using a Leica EM-VCM500 machine and a cryo-holder suitable for c-clipped grids (Leica Microsystems, Vienna, Austria). Prior to sample loading into the FIB-SEM microscope ...
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bioRxiv - Neuroscience 2023Quote: ... for 48 hours at 4°C before being cut into 30 μm thick coronal serial sections on a sliding microtome (Leica Biosystems, Wetzlar Germany). The free-floating brain sections were then stored in antifreeze solution at −20°C until immunohistochemical staining.
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bioRxiv - Neuroscience 2023Quote: ... snap-frozen in powdered dry ice and stored at -80 °C until sectioned at 12 µm in the coronal plane with a Leica CM1860 UV cryostat (Leica Biosystems, Wetzlar, Germany). Sections corresponding to Atlas plates 20-26 of Paxinos (Bregma +1.34 to +0.62 mm ...
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bioRxiv - Genomics 2023Quote: ... and sliced into 13 µm slices using PTFE-coated microtome blades (Duraedge, 7223) at -10°C on a Cryostat CM1860 (Leica, 14-0491-46884). Liver sections were mounted on VWR Superfrost Plus slides (VWR ...
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bioRxiv - Systems Biology 2023Quote: ... the mouse brains were sectioned coronally into 40 μm-thick slices at −18 to −20 °C using a microtome (Leica Microsystems, Wetzlar, Germany). Floating brain sections (fixed in 4% PFA ...
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bioRxiv - Genomics 2023Quote: Endometrial biopsies were fixed overnight in 10% neutral buffered formalin at 4°C and wax embedded in Surgipath Formula ‘R’ paraffin (Leica BioSystems, Wetzlar, Germany) using the Shandon Excelsior ES Tissue processor (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... the specimens were washed with PBS and were stored at 4 °C until the images were taken using a confocal microscope (Leica-SP8, Leica, Germany) using a 20× air-dry objective.
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bioRxiv - Cancer Biology 2023Quote: ... slides were baked for 2 hours at 65°C for paraffin removal and subsequently loaded onto a Leica BOND RX (Leica Biosystems, Wetzlar, Germany) for tissue rehydration in EtOH and ddH2O ...
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bioRxiv - Neuroscience 2023Quote: ... P14-P16 and P20-P21 pups of both sexes were placed in a perfused chamber at room temperature (22-25°C) in oxygenated Mg2+ containing ACSF (ACSF-1) medium under a Leica DMLFS microscope (Leica Microsystems Inc., USA). The recordings were performed using a coated nichrome electrode with an Iso-DAM8A World Precision Instruments amplifier (World Precision Instruments ...
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bioRxiv - Cell Biology 2023Quote: ... Experiments were performed at 37°C with 5% CO2 using a total internal reflection fluorescence (TIRF) microscope (Leica AM TIRF inverted microscope; Leica Microsystems, Wetzlar, Germany). Image sequences of individual particles (500 frames ...
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bioRxiv - Immunology 2020Quote: ... and rabbit anti-rat secondary (Vector) DAPI was used to stain nuclei in Bond Polymer Refine Detection (Leica Biosystems) according to manufacturer’s protocol ...
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bioRxiv - Biophysics 2020Quote: ... the hind wings are dehydrated and cut into six pieces at the nanoindentation test points at the C+ScA after being embedded in paraffin using a paraffin embedding machine (Leica EG1150C, Leica Biosystems, Germany) to support the specimens in the slicing test ...
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bioRxiv - Developmental Biology 2021Quote: High-pressure frozen samples were vacuumed to 5 × 10−7 mbar at -120 °C and a fracture was conducted at the interface between the two HPF discs containing the sample (BAF 60, Leica Micro-systems, Vienna, Austria). One disc containing the exposed cryo-fixed ...
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bioRxiv - Neuroscience 2022Quote: ... containing the primary somatosensory cortex (S1) were cut in ice-cold (4 °C) NMDG ACSF using a Leica VT1200s vibratome (Leica Biosystems, Buffalo Grove, IL) and transferred to warm (33 °C ...
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bioRxiv - Genomics 2020Quote: ... individuals were lethally anaesthetized using benzocaine bath (150 mg.L−1) and a lateral photograph was taken using a digital fixed camera (Leica Microsystems; Figure 1B and C). Microbiome and host sampling consisted in gentle fish skin smears with sterile swabs ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The animals were given cold shock for 2 minutes at -4°C and then photographed along with a reference scale under a stereo zoom microscope (Leica DFC 290, Leica Microsystems GmbH, Wetzlar, Germany) with a mounted digital camera ...
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bioRxiv - Neuroscience 2020Quote: ... Sagittal sections (25 μm thick) were cut using a Leica CM1900 cryostat at a chamber temperature of −20 °C (Leica Biosystems, Buffalo Grove, IL) and mounted onto Superfrost-Plus Gold microscope slides (Fisher Scientific ...
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bioRxiv - Plant Biology 2020Quote: ... Plants were watered and left at ambient conditions (24 °C) with lid on top and imaged two days past transformation with an SP8 confocal laser scanning microscope (CLSM) (Leica Microsystems GmbH, Wetzlar, Germany) with LAS AF and SymPhoTime software (Picoquant GmbH ...
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bioRxiv - Molecular Biology 2020Quote: ... 4 μl of this mixture was back side blotted for 6 s at relative humidity 98% and temperature 19°C on a glow-discharged holey carbon grid (CF-2/1-3C, Protochips) before plunge-freezing in liquid ethane (Leica EM GP2 automatic plunger). Dose-symmetrical tilt series acquisition 51 was performed on an FEI Titan Krios electron microscope operated at 300 kV using a Gatan Quantum energy filter with a slit width of 20 eV and a K2 or K3 direct detector operated in counting mode ...
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bioRxiv - Cell Biology 2020Quote: ... The fresh frozen brain samples were sectioned sagittally at 14 μm thickness and the frozen brain homogenates at 20 μm thickness with a Leica CM3050 cryostat at −20°C (Leica Microsystems GmbH, Wentzler, Germany). All brain specimens were cut at approximately the same Bregma in order to clearly delineate the hippocampus ...
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bioRxiv - Cell Biology 2022Quote: ... The plates containing the pseudostratified epithelia were incubated at 37 °C with 5% CO2 in the 3i Marianis/Yokogawa Spinning-Disk Confocal microscope (Leica Microsystems, Sugar land, USA) as reported in our previous publications (53 ...
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bioRxiv - Biophysics 2022Quote: ... Confocal images and super-resolution images (Figure 3A, C, D; Figure S2A) were acquired under 100× objectives on a STED confocal microscope (Leica TCS SP8 STED 3X). Images (Figure S2C ...
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bioRxiv - Cancer Biology 2021Quote: ... After heat-induced epitope retrieval (HIER) at 100°C (BOND Epitope Retrieval Solution 1 or 2, Leica, Germany; HIER 1 AR9961, HIER 2 AR9640), endogenous peroxidase activity was blocked by incubation with 3% peroxide block for 20 minutes (BOND Polymer Refine Detection System ...
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bioRxiv - Microbiology 2021Quote: ... pre-warmed at 37°C and the channels were imaged at x64 magnification using a DM-IRE2 confocal laser scanning microscope (Leica Microsystems Heidelberg GmbH, Germany) and the accompanying Leica Microsystems Confocal Software (version 2.61 Build 1537) ...
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bioRxiv - Biophysics 2022Quote: ... nobilis prisms from the prismatic layer of the pinna nobilis shell were fixed along the c-axis of calcite with DI water under -20°C then cut into slices with a thickness of around 30 µm using a Leica Microtome (Leica CM3050 S, Leica Microsystems Nussloch GmbH, Germany). To separate the organic shields ...
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Decoupling cell size homeostasis in diatoms from the geometrical constraints of the silica cell-wallbioRxiv - Microbiology 2023Quote: ... Vitrified samples were kept in liquid nitrogen until freeze-substitution in a freeze-substitution device at −90 °C (EM ASF2, Leica Microsystems GmbH, Wetzlar, Germany). For 48 hours ...