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Citations for Leica :
601 - 650 of 775 citations for 6 FLUORO 4 METHYL 3 PHENYLCOUMARIN since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Processed tissues were embedded in paraffin and sectioned at 4 microns on a rotary microtome (Leica Biosystems, Buffalo Grove, IL). Tissues were mounted on glass slides and stained with hematoxylin and eosin using routine protocols on an automated histostainer (Leica ST5010 Autostainer ...
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THE OLFACTORY RECEPTOR Olfr78 REGULATES DIFFERENTIATION OF ENTEROCHROMAFFIN CELLS IN THE MOUSE COLONbioRxiv - Cell Biology 2023Quote: ... overnight at +4°C and then sedimented through 20% and 30% sucrose solution sequentially (minimum 24 hours each) before OCT (Leica) embedding ...
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bioRxiv - Genomics 2023Quote: ... each liver sample was exhaustively sectioned into 4 μm-thick sections by a fully automated rotary microtome (Leica RM2255, Germany). Each of these sections was stained with hematoxylin and eosin and mounted ...
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bioRxiv - Neuroscience 2023Quote: Brain tissues after electrophysiology brain slice preparation were fixed in 4% PFA in 0.1 M phosphate buffer overnight at 4 °C and then were resected into 70–100 μm slices using a VT1000s vibratome (Leica Biosystems ...
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bioRxiv - Synthetic Biology 2023Quote: ... Transgenic fish were screened at 2-4 dpf for CFP expression in the eyes using a CFP filter (Leica, 10447409) on a fluorescence stereomicroscope (Leica ...
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Mitigating a TDP-43 proteinopathy by targeting ataxin-2 using RNA-targeting CRISPR effector proteinsbioRxiv - Bioengineering 2023Quote: ... The harvested tissues were then subsequently fixed with 4% paraformaldehyde (v/v) at 4°C and sliced into 40-μm sagittal and axial sections using a CM3050 S cryostat (Leica).
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bioRxiv - Developmental Biology 2023Quote: ... Undecalcified frozen sections at a thickness of 4 µm were obtained according to Kawamoto’s film method using TC-65 (Leica Microsystems) or SL-T35 (UF ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were stored in a 4% formaldehyde and 30% sucrose solution for at least 1 week before slicing with a freezing microtome (Leica) at 50uM ...
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bioRxiv - Genomics 2024Quote: ... Sections of 4 μm without wax were stained with safranin and fast green and photographed with electric inverted fluorescence microscope (MDi8, Leica).
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bioRxiv - Bioengineering 2023Quote: ... The brains were extracted and postfixed for 24-48 hours in the same fixative at 4°C before being sliced into 50 μm coronal sections using a vibratome (Leica). The slices were blocked in 10% normal goat serum (SouthernBiotech ...
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bioRxiv - Neuroscience 2024Quote: ... The brains were kept in 4% PFA at least overnight and stored in PBS until histological slicing (Leica VT1200 S). DAPI-Fluoromount-G (70-100 µm slices ...
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bioRxiv - Neuroscience 2024Quote: ... Cortices were included in 4% agar and tangential sections were cut at 100 µm on a vibrating microtome (Leica, VT1000S).
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bioRxiv - Cancer Biology 2024Quote: ... Cryo-embedded mouse tumors (OCT compound, Tissue-Tek, #14291) were cut to 4-5µm with a cryostat (Leica CM 1950, Leica Biosystems) and positioned onto SuperFrost plus slides (R.Langenbrinck ...
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bioRxiv - Neuroscience 2024Quote: ... electroporated cerebella were embedded in 4% LMP agarose dissolved in mHBSS and then cut into 300 mm sagittal slices using a VT1200 vibratome (Leica). The slices were finally placed on 0.4 mm PTFE cell culture inserts (Millicell ...
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bioRxiv - Neuroscience 2024Quote: ... Stained sections were stored at 4°C and imaged using the Inverted Leica DMIL LED Microscope with the Thunder imaging software (Leica).
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bioRxiv - Microbiology 2019Quote: Purified RNA was stained with cyanine 3 or cyanine 5 using the ULS Fluorescent Labeling Kit for Agilent arrays from Kreatech (Leica Biosystems, Amsterdam, Netherlands) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... Tissue blocks were then embedded in 3% agarose gel and cut in the sagittal plane at 30 μm-thickness by the vibratome (Leica, Buffalo Grove, IL), counter-stained with Dapi (1:2000 dilution ...
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bioRxiv - Cancer Biology 2020Quote: Super-Resolution Stimulated Emission Detection (STED) microscopy (Figure 1A, Figure S1, Figure 4A) was performed using a commercial Leica SP8 STED 3× system (Leica Microsystems, Mannheim, Germany), equipped with a white light laser with continuous spectral output between the wavelengths of 470 nm to 670 nm ...
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bioRxiv - Neuroscience 2020Quote: ... whereas slices processed for immunofluorescence were embedded in 3% agarose solution and re-sliced at 40-µm thickness using a vibratome (Leica VT1200S; Leica Microsystems; Bannockburn, IL).
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bioRxiv - Neuroscience 2020Quote: ... the brains were embedded in 3% agar and cut into 40 μm coronal visual cortical sections using a vibratome (Leica Biosystems, Wetzlar, Germany). After washing with PBST (1x PBS and 0.2% TritonX100) ...
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bioRxiv - Plant Biology 2021Quote: ... Cells cultured in M9 or LS medium containing 3% sucrose were added to flat specimen carriers and frozen using a high-pressure freezing machine (Leica EM PACT, Leica Microsystems, Wetzlar, Germany). The frozen samples were transferred to 2% osmium tetroxide in anhydrous acetone at -80 °C and incubated at -80 °C for 6 days (120 h) ...
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bioRxiv - Neuroscience 2020Quote: ... Representative images were taken from the cortical layer 2-3 with Nikon-DS Fi3 camera attached to a Leica DM 2000 Led light microscope (Leica Microsystems GmbH, Germany).
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bioRxiv - Neuroscience 2020Quote: ... Neurons were transduced with LV-Syn1-GFP at DIV 3 and were imaged at DIV 21 using an inverted spinning disk confocal microscope (Leica DMi8, 63x objective). During image recording the cells were maintained in a humidified atmosphere of 5% CO2 at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... The brain was then embedded in 2 to 3% agar and 100 μm thick sections were cut using a microtome (Leica VT1000 S vibratome). Slices were mounted using a Vectashield HardSet mounting medium containing DAPI (H-1500-10 ...
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bioRxiv - Developmental Biology 2019Quote: ... Subsequently the samples were immersed in 100% ethanol for 3×10 min and then critical point dried in an Automated Critical Point Dryer Leica EM CPD300 (Leica Microsystems, Vienna, Austria) with 18 steps of ethanol - CO2 exchange.
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bioRxiv - Neuroscience 2021Quote: ... Samples were freeze substituted in acetone containing 0.1% uranyl acetate plus 3% distilled water in a freeze substitution unit (Leica EM AFS2, Leica Microsystems, Buffalo Grove, IL) at −90°C for 20 hours ...
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bioRxiv - Neuroscience 2023Quote: Dual or triple channel STED imaging of Ib and Is AZs was carried out using a Leica SP8 confocal/STED 3× microscope (Leica Microsystems, Mannheim, Germany) with 100X ...
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bioRxiv - Neuroscience 2022Quote: ... We prepared horizontal brain slices containing the somatosensory S2 cortex and the corresponding corticostriatal projection field in the dorsal striatum from mice (2-3-months old) using a vibrating blade microtome (VT1200S, Leica Micosystems, Nussloch, Germany). Brains were sliced in a 5% CO2/95% O2-bubbled ...
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bioRxiv - Cancer Biology 2023Quote: ... rehydrated and subsequently stained with hematoxylin and eosin (H&E) and appropriate antibodies (Supplementary Table 3) using the automated system BOND RX (Leica Biosystems, Newcastle, UK). Visualization was by the Bond Polymer Refine Detection kit (Leica Biosystems ...
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bioRxiv - Neuroscience 2023Quote: ... Larvae were placed into a petri dish filled with HL-3 on ice and imaged (camera: Leica DFC365 FX, microscope: Leica MZ10 F). Brains were stained against HRP ...
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bioRxiv - Developmental Biology 2021Quote: ... EBs or brain tissues were fixed in 4% paraformaldehyde for 7-8 hours at 4°C followed by equilibration in 20% sucrose solution overnight and embedded in tissue freezing medium (Leica, 14020108926). The embedded tissues were then cryo-sectioned and collected on Superfrost slides (Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... when embryos were fixed by 4% PFA for ensuing WISH analysis or imaged using a Fluorescent stereoscopic microscope (LEICA M205 FCA). 10 pg of gsc mRNA and 10 pg of ndr2 mRNA were injected ...
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bioRxiv - Biochemistry 2020Quote: ... but the purified V2R-Gs-Nb35 complex was at a concentration of 4 mg/ml and the cryo-EM grids were prepared using a EM GP2 (Leica microsystems) plunge freezer with a 4s blotting time (100% humidity and 4°C).
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bioRxiv - Biochemistry 2020Quote: ... and allowed to set O/N at 4 °C before being imaged using a SP8 white-laser confocal microscope (Leica Microsystems). Filters were adjusted specifically for each stain to visualize nuclei (Hoechst ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were isolated and immersed in 20% sucrose for 1 day and 30% sucrose for 2 days at 4°C before being sectioned at 40 μm thicknesses on a cryostat microtome (Leica, CM3050s). Brain slices were collected and blocked with blocking solution containing 2% Normal goat serum (v/v) ...
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bioRxiv - Molecular Biology 2021Quote: ... The paraffin blocks transferred to room temperature and the blocks sections cut 4 mm thick in a rotatory microtome (Leica RM2135) and mounted in glass slides ...
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bioRxiv - Neuroscience 2021Quote: ... mouse brain hemispheres prefixed with 4% PFA for 48 hours were sectioned at 50 μm using a Vibratome (Leica Microsystems Inc). Immunostaining of free-floating sections was performed using the ABC kit (Vector Laboratories ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were dehydrated in sucrose (15% and 30%, two days) at 4°C and sectioned at 40 μm thicknesses on a cryostat microtome (Leica, CM3050s). The brain slices were collected into three sets ...
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bioRxiv - Neuroscience 2020Quote: ... The brains were removed and post-fixed in PBS containing 4% paraformaldehyde over night before cutting 50μm thick coronal sections on a vibratome (VT 1000 S from Leica, Wetzlar, Germany). Floating sections were permeabilized with 2% Triton X-100 in PBS overnight and blocked with 2% normal goat serum (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... the proximal and distal 2-3 mm of LCCA were discarded and the remaining portion (4-5 mm) were embedded in optimal cutting temperature compound for frozen sectioning on a cryostatmicrotome (Leica Biosystems). Frozen sections (10 μm thick ...
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bioRxiv - Neuroscience 2020Quote: ... pH 7.4 with 5% CO2/ 95% O2) and whole brain sagittal slices (300 µm) were cut using a vibratome (VT1200, Leica Biosystems). Afterwards slices were transferred to 34°C cutting solution for 45 minutes to recover and finally maintained at RT until used for recordings.
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bioRxiv - Bioengineering 2021Quote: ... Fixed brains were incubated in 100 mM glycine for 1-2hrs at 4°C and sectioned to 10 um-thick slices with a vibratome (Leica VT1000S).
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bioRxiv - Neuroscience 2020Quote: ... Brains were embedded in 4% low-melting agarose and coronal cortical slices were generated (300 µm) with a vibrating microtome (VTS1000 Leica Microsystems). Slices were next incubated for 60 minutes at 37°C in DMEM/F-12 imaging media without phenol red supplemented with 10% fetal bovine serum (FBS).
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bioRxiv - Neuroscience 2020Quote: Brains were embedded in agar (3-4% w/v dissolved in dH2O) before being cut into 50 μm coronal sections on a vibrating microtome (VT1000S; Leica Microsystems). Free-floating tissue sections were collected in series ...
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bioRxiv - Cell Biology 2021Quote: ... The livers were mounted in 4% low-melting agarose in PBS and cut into 100 μm – thick sections at a vibratome (Leica VT1200S). For deep tissue imaging ...
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bioRxiv - Neuroscience 2020Quote: ... Sections of 300μm thickness were embedded into 4% low melting agarose and further cut into 50 μm slices with vibrating-blade microtome (Leica, Germany). Nuclei were counterstained by adding a small drop of ProLong™ Gold Antifade Mountant with DAPI (Invitrogen™ ...
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bioRxiv - Neuroscience 2020Quote: Brain organoids (4.5 months old) were embedded into 4% low melting agarose and 300 μm slices were cut with vibrating-blade microtome (Leica, Germany). Slices were prepared using ice-cold artificial cerebrospinal fluid (ACSF ...
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bioRxiv - Neuroscience 2022Quote: ... It was then stored in 30% sucrose solution with 0.5% sodium azide at 4°C until preparation of 40 µm-thick coronal sections using a freezing microtome (SM 2010R, Leica Biosystems). Four to eight sections per mouse through the dorsal hippocampal region were used to evaluate hippocampal sclerosis by cresyl violet staining or gliosis by glial fibrillary acidic protein (GFAP ...
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bioRxiv - Cell Biology 2022Quote: ... Cell layers were embedded in paraffin and 4 μm thick sections were made using a Leica RM2165 microtome (Leica, Wetzlar, Germany). Alcian Blue/Periodic Acid-Schiff (AB/PAS ...
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bioRxiv - Cell Biology 2022Quote: ... The imaged area was marked with a circle (4 mm in diameter) around the center of the imaged area using an objective diamond scriber (Leica, 11505059)39 ...