Labshake search
Citations for Leica :
301 - 350 of 775 citations for 6 FLUORO 4 METHYL 3 PHENYLCOUMARIN since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2019Quote: ... Brains were embedded in 6% agarose and sectioned coronally at 30-micron thickness using a Leica VT1000S vibratome (Leica Camera, Wetzlar, Germany).
-
bioRxiv - Molecular Biology 2020Quote: ... the number of colonies exhibiting correct fluorescence were counted (EGFP+ for 2-insert assembly, EGFP+/mCherry+ for 6-insert assembly) using a fluorescence stereoscope (Leica M165 FC). Accuracy was calculated as the percentage of colonies exhibiting correct fluorescence ...
-
bioRxiv - Bioengineering 2022Quote: ... The distribution of cells in the microgel matrix was assessed after 6 h using a Leica SP8 DIVE multiphoton microscope (Leica Microsystems, Germany) with 16x water immersion lens ...
-
bioRxiv - Plant Biology 2022Quote: ... Nodule primordia and nodules were embedded in 6% low-melting agarose and sliced into 40 - 50 µm thick sections using a vibrating-blade microtome (Leica VT1000 S).
-
bioRxiv - Neuroscience 2022Quote: ... the fresh retinal wholemount was embedded in 6% agar and cut into 250-μm slices with a vibrating blade microtome (Leica VT100 S). For nocodazole treatment ...
-
bioRxiv - Cell Biology 2022Quote: ... the 6-well plate containing the cells were mounted onto a confocal microscope (Leica TCS SP8 with a 10Å∼0.4 NA objective). They were then maintained at 37ºC (stage heater ...
-
bioRxiv - Molecular Biology 2023Quote: ... Cells were mounted on stubs using colloidal silver as an adhesive and sputter coated with 6 nm gold (Leica Coater ACE 200). Cells were imaged using FEI Quanta 3D scanning electron microscope operated at an accelerating voltage of 2 kV ...
-
Phospholipase C β4 promotes RANKL-dependent osteoclastogenesis by interacting with MKK3 and p38 MAPKbioRxiv - Cell Biology 2024Quote: ... The resin blocks were cut longitudinally into 6-μm slices of the tibia using a Leica RM2165 rotary microtome equipped with a tungsten blade (Leica Microsystems, Germany). Fluorescence signals emitted by calcein green and alizarin red were recorded using a fluorescence microscope (Leica Microsystems) ...
-
bioRxiv - Microbiology 2020Quote: ... 2.3% methanol and 1–3% H2O in Acetone) tempered at −90°C in FS device (Leica AFS2) equipped with a robotic solution handler (Leica FSP) ...
-
bioRxiv - Plant Biology 2019Quote: ... Expression of the appropriate construct was analysed after 3 days by confocal laser-scanning microscopy (Leica SP8) with excitation lights of 405 nm for CFP and 488 nm for GFP.
-
bioRxiv - Cell Biology 2020Quote: ... Images were prepared with the Leica Application Suite – Advanced Fluorescence (LAS-AF) Version 3 software (Leica Microsystems).
-
bioRxiv - Biophysics 2019Quote: ... we imaged cilia dynamics with 3 different microscopes (two commercial spinning disk microscopes from 3i and Leica, and one 2-photon scanning microscope using the scanning artifact ...
-
bioRxiv - Neuroscience 2020Quote: ... the Ca2+ signal within the SNs was captured during 3-min using Leica DM6000 microscope (Leica Microsystems) and processed with the software Fiji image J ...
-
bioRxiv - Neuroscience 2020Quote: ... slices were mounted in 3% agarose and sliced using a vibratome (Leica VT1200S; Leica Microsystems; Bannockburn, IL) to a thickness of 30 µm ...
-
bioRxiv - Cell Biology 2020Quote: ... The surface area of cardiomyocytes stained by MHC was determined with image analysis software (Leica Qwin 3) and calculated as the mean of 100 to 120 cells from randomly selected fields.
-
bioRxiv - Cell Biology 2021Quote: ... were recorded and the maximal projection was deconvoluted with 3 iterations by the LAS AF 2.7.3.9 software (Leica).
-
bioRxiv - Biochemistry 2021Quote: ... for 3 sec before plunge freezing in liquid ethane using a Leica EM Grid Plunger (Leica Microsystems). Datasets were collected at the NIH Multi-Institute Cryo-EM Facility (MICEF ...
-
bioRxiv - Molecular Biology 2019Quote: ... A 30 second video recording was captured using a 3 MP DFC290 HD digital camera (Leica Microsystems) at a rate of 30 frames per second ...
-
bioRxiv - Physiology 2020Quote: ... mexicanum gametes was imaged using FluoZin-3 and a TCS SP5 confocal microscope (Leica Microsystems, Wetzlar, Germany) equipped with a Leica 506224 5X objective ...
-
bioRxiv - Neuroscience 2020Quote: ... Bregma −1.5 to −3) were cut in ice cold carbogenated cutting solution using a vibratome (VT1200S, Leica). Slices were incubated in cutting solution in a submerged chamber at 34°C for at least 45 min and then transferred to ACSF in a light-shielded submerged chamber at room temperature (21°C ...
-
bioRxiv - Cancer Biology 2019Quote: ... Positive nuclei (Ki67) and stained area (cleaved caspase 3) were analyzed using the ImageScope software (Leica Biosystems). Quantification is average of 3 sections ...
-
bioRxiv - Neuroscience 2021Quote: ... Bregma −1.5 to −3) were cut in ice cold carbogenated cutting solution using a vibratome (VT1200S, Leica). Slices were incubated in cutting solution in a submerged chamber at 34°C for at least 45 min and then transferred to ACSF in a light-shielded submerged chamber at room temperature (21°C ...
-
bioRxiv - Neuroscience 2021Quote: ... Brains were sliced in 30 μm coronal sections into 3 series on a cryostat (Leica CM3050 S) and stored free-floating in antifreeze cryoprotectant [0.25 mM polyvinylpyrolidone ...
-
bioRxiv - Cell Biology 2022Quote: Cells attached to 3-mm sapphire discs were cryo-immobilized using an EM ICE (Leica Microsystems, Austria) and 6-mm sapphire discs were frozen by using a Compact 03 high-pressure freezer (M ...
-
bioRxiv - Plant Biology 2022Quote: ... 1 cm root segments were embedded in 3% agarose and sectioned using a vibratome (Leica VT1000 S) to produce 150-200 μM thickness sections ...
-
bioRxiv - Neuroscience 2022Quote: ... They were embedded in 3% agar and 200 µm thick sections cut using vibratome (VT1000S, Leica, GmbH). Sections were mounted onto gelatin-coated slides ...
-
bioRxiv - Cell Biology 2022Quote: ... and images were acquired using a 3-channel TCS SP2 laser-scanning confocal microscope (Leica Mycrosystems, Germany) and STELLARIS 8 ...
-
bioRxiv - Molecular Biology 2023Quote: 2.8 × 105 A549 or A549-IFITM3 cells were seeded on carbon- coated 3 mm sapphire discs (Leica) in a 6-well plate in complete growth medium (DMEM-F12 ...
-
bioRxiv - Neuroscience 2023Quote: ... the puncture sample was placed in an aluminum specimen carrier (Ø = 3 mm; Leica Microsystems, Wetzlar, Germany) and underwent freezing in a high-pressure freezer (HPM100 ...
-
bioRxiv - Developmental Biology 2024Quote: ... larvae were imaged in 3% methylcellulose on a binocular microscope using Leica Application Suite (LAS 3.3, Leica). After imaging ...
-
bioRxiv - Neuroscience 2023Quote: ... Brains were embedded in 3% agarose and cut into 100μm thick sections using a VT1000S vibratome (Leica). Sections were incubated O/N in primary antibody solution containing 5% NGS and 0.3% triton ...
-
bioRxiv - Cell Biology 2020Quote: ... Brain images were acquired in a 4% PFA solution using a stereomicroscope (M80, Leica Microsystems).
-
bioRxiv - Bioengineering 2021Quote: ... DLP-printed moulds were sputter-coated with 4 nm platinum (Leica EM ACE600, Wetzlar, Germany) And imaged with a Phenom XL G2 Desktop SEM (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2021Quote: ... They were kept at 4 °C until cutting at the cryostat (CM3050S; Leica Wetzlar, Germany). We produced 20-μm thick coronal sections of the primary motor cortex (M1 ...
-
bioRxiv - Neuroscience 2023Quote: Brains perfused in 4% PFA were sectioned into 80 µm slices using a Vibratome (Leica), then mounted and stained with cresyl violet to reconstruct lesion size ...
-
bioRxiv - Immunology 2023Quote: ... 4 μm-thick segments of the fragments were prepared in a semiautomatic microtome (Leica®) and organized on microscopic slides ...
-
bioRxiv - Biophysics 2023Quote: ... Ultrathin sections (60 -80 nm) were obtained using Leica UCT 4 ultramicrotome (Leica, Wetzlar, Germany) and stained with uranyl acetate ...
-
bioRxiv - Cell Biology 2023Quote: ... The 4 µm plastic sections were cut with a rotational microtome (Leica Microsystems, Wetzlar, Germany) and stained with hematoxylin and eosin (HE ...
-
bioRxiv - Molecular Biology 2024Quote: ... Mounted slides were stored at 4°C until imaged using LAS AF software (Leica Microsystems) on a Leica TCS SP8 confocal microscope ...
-
bioRxiv - Neuroscience 2021Quote: ... then sectioned coronally into 20-μm-thick slices (1:6 series of the PVN) using a Leica CM1900 cryostat (Leica Microsystems, Wetzlar, Germany). Immunofluorescence staining was performed on brain sections derived from P10 male tdTomato-Crh (Crh-IRES-Cre;Ai14 ...
-
bioRxiv - Neuroscience 2021Quote: ... isogenic samples reared in the same environment were highpressure frozen (Leica HPM100 for datasets 1-5 and Leica ICE for datasets 6-8) at different stages of post-embryonic development ...
-
bioRxiv - Plant Biology 2020Quote: ... Dried samples were sputter-coated with argon–platinum plasma at a distance of 6–7 cm and 45 mA intensity for 15 sec in a sputtering chamber (Leica Microsystems EM MED020). Scanning electron micrographs were acquired using an AURIGA compact FIB-SEM (Zeiss ...
-
bioRxiv - Cell Biology 2021Quote: ... resin blocks were mounted onto SEM pins with silver epoxy and sputter coated with 6 nm of iridium (Leica ACE 600, Vienna, Austria) at a 45° angle with rotation on a planetary stage to ensure the entire block was coated.
-
bioRxiv - Neuroscience 2023Quote: ... Brain regions containing the possible contact sites were re-embedded into resin blocks and 70 nm thick sections were cut with an ultra-microtome (Leica EM UC 6). Electron microscopic images were taken either with a Hitachi H-7100 transmission electron microscope or a FEI series electron microscope to reveal synaptic contacts between BFPVNs and target cell types in the MS ...
-
bioRxiv - Cancer Biology 2024Quote: ... Antigen retrieval was performed in BOND Epitope Retrieval Solution 1 (pH 6) or 2 (pH 9) (ER1, ER2, Leica Biosystems, Cat. AR9961, AR9640) at 95°C ...
-
bioRxiv - Neuroscience 2020Quote: ... embedded with agarose 3% in PBS 0.01M and cut in slices of 80μm of thickness in a vibratome (Leica). Slices were incubated for 1h at room temperature in a blocking solution containing 1% BSA and 0.3% Triton X-100 in PBS 0.01M ...
-
bioRxiv - Neuroscience 2020Quote: ... 5-6 stacks (184.58µm x 184.58µm each) from layers 2/3 of the motor cortex were obtained using high resolution confocal microscopy (Leica TCS SP8 ...
-
bioRxiv - Neuroscience 2020Quote: ... embedded in 3% agarose-PBS and cut 50–100 μm in thickness using a vibrating microtome (Leica, VT100S). Sections were placed in blocking solution containing 10% Normal Goat Serum (NGS ...
-
bioRxiv - Neuroscience 2021Quote: ... embedded in 3% agarose-PBS and cut 50–100 μm in thickness using a vibrating microtome (Leica, VT100S). Sections were placed in blocking solution containing 10% Normal Goat Serum (NGS ...
-
bioRxiv - Immunology 2021Quote: ... Tissues were routinely processed for histopathology on a Leica Peloris 3 Tissue Processor (Leica Biosystems, Buffalo Grove, IL), embedded in paraffin ...