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Citations for Leica :
51 - 100 of 1729 citations for 6β Fluoro 21 hydroxypregna 1 4 9 11 16 tetraene 3 20 dione 21 acetate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Worms were freeze substituted for 2 h at −90 °C with 1% osmium tetroxide and 0.1% uranyl acetate in acetone using a Leica EM AFS2 freeze substitution processor (Leica Microsystems). After a temperature ramp to 0°C ...
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bioRxiv - Synthetic Biology 2019Quote: ... cell monolayers were ultrathin sectioned at 80 nm on formvar-coated copper grids (Plano) and postcontrasted using 1 % uranyl acetate and ultrostain (Leica). TEM images for Fig ...
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bioRxiv - Cell Biology 2022Quote: ... Sections were stretched with chloroform to eliminate compression and mounted on Pioloform filmed copper grids prior to staining with 1% aqueous uranyl acetate and lead citrate (Leica). They were viewed on a Philips CM100 Compustage Transmission Electron Microscope with images collected using an AMT CCD camera (Deben) ...
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bioRxiv - Cell Biology 2020Quote: ... Ultrathin sections (50nm) were deposited onto formvar-coated copper grids (Plano) and postcontrasted using 1% uranyl acetate in water and ultrostain (Leica). TEM images were acquired on a JEM 1400plus (JEOL ...
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bioRxiv - Immunology 2021Quote: ... were cut and deposited onto formvar-coated grids (Plano) and again contrasted using 1 % uranyl acetate in water and Ultrostain (Leica). Images were acquired on a JEM 1400plus (JEOL).
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bioRxiv - Immunology 2023Quote: ... featuring the excitation bandpass filters 340/26 and 387/11 (Leica). The total recording time was 10-20 minutes with an image recorded every 15-20 seconds ...
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bioRxiv - Neuroscience 2024Quote: ... and stained in uranyl acetate and lead citrate (Leica EM AC20). Sections were imaged in a JEOL1010 transmission electron microscope (JEOL ...
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bioRxiv - Neuroscience 2024Quote: ... Videos of Day 3 adult worms for at least 20 seconds was recorded using stereo-zoom microscope (Leica) 28 ...
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bioRxiv - Biochemistry 2023Quote: ... Rat hippocampus tissue punches and thin sections were prepared according to published protocols.33 Rat brain hemisphere slices 16 µm thick were cut at -16°C using a cryostat (Leica CM3050 S ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were sectioned at 16 μm (Leica, CM3050S) and mounted on glass slides stored at −20°C ...
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bioRxiv - Molecular Biology 2020Quote: ... and sectioned sagittally at 9 µm using a CM3050S cryostat (Leica). Prior to immunodetection ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were stained in 2% aqueous uranyl acetate and lead citrate (Leica) and imaged on a Hitachi HT7800 transmission electron microscope ...
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bioRxiv - Cell Biology 2022Quote: ... After automated staining with uranyl acetate and lead citrate (Leica EM AC20), samples were observed via either Zeiss TEM 902 A (50 kV ...
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bioRxiv - Cancer Biology 2023Quote: ... stained with uranyl acetate and lead citrate in an EM Stainer (Leica), and examined using a JEM 1010 transmission electron microscope (JEOL ...
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bioRxiv - Plant Biology 2023Quote: ... post-stained with uranyl acetate and lead citrate with an EMSTAIN (Leica) and observed with a Libra 120 transmission electron microscope (Carl Zeiss Microscopy GmbH ...
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bioRxiv - Biophysics 2019Quote: Samples were freeze-substituted in 1% osmium tetroxide and 0.1% uranyl acetate in acetone using a Leica AFS (Leica Microsystems, Vienna, Austria) following a previously described protocol [52] ...
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bioRxiv - Cell Biology 2021Quote: ... Freeze substitution was performed over 3 d at −90°C in anhydrous acetone containing 1% OsO4 and 0.1% uranyl acetate using an automatic freeze substitution machine (EM AFS, Leica Microsystems, Vienna, Austria). Epon/Araldite infiltrated samples were then embedded in a thin layer of resin and polymerized for 3 d at 60°C ...
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bioRxiv - Cell Biology 2020Quote: ... Freeze substitution was performed over 3 days at –90 °C in anhydrous acetone containing 1 % OsO4 and 0.1 % uranyl acetate using an automatic freeze substitution machine (EM AFS, Leica Microsystems, Vienna, Austria) Serial semi-thick sections (300nm ...
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bioRxiv - Cell Biology 2021Quote: ... Freeze substitution was performed over 3 d at -90 °C in anhydrous acetone containing 1 % OsO4 and 0.1 % uranyl acetate using an automatic freeze substitution machine (EM AFS, Leica Microsystems, Vienna, Austria). Epon/Araldite infiltrated samples were flat embedded in a thin layer of resin ...
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bioRxiv - Cell Biology 2024Quote: ... The tissues in paraffin blocks were subjected to sectioning (3-4 μM) by rotary type of microtome (Leica RM2255 Rotary Microtome ...
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bioRxiv - Neuroscience 2021Quote: ... 16-bit confocal images (Leica TCS SP8 confocal microscope) were collected and processed in Fiji (ImageJ ...
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bioRxiv - Cancer Biology 2021Quote: ... 9-14 sections per case were mounted on PEN membrane slides (Leica) and lightly stained with haematoxylin to facilitate microscopic identification of tumor and stroma areas ...
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bioRxiv - Neuroscience 2021Quote: ... in 0.1M phosphate buffered saline (PBS, Fisher #BP399-20) at +4°C before slicing on a freezing microtome (Leica) in a 1:12 series of 40 µm thick coronal sections ...
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bioRxiv - Neuroscience 2021Quote: ... and sections post-stained with 0.5% uranyl acetate (Leica Ultrostain I, Leica Microsystems) and 3% lead citrate (Leica Ultrostain II ...
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bioRxiv - Neuroscience 2021Quote: ... and sections post-stained with 0.5% uranyl acetate (Leica Ultrostain I, Leica Microsystems) and 3% lead citrate (Leica Ultrostain II ...
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bioRxiv - Biochemistry 2023Quote: ... Rat hippocampus tissue punches and thin sections were prepared according to published protocols.33 Rat brain hemisphere slices 16 µm thick were cut at -16°C using a cryostat (Leica CM3050 S, Leica Biosystems GmbH, Wetzlar, Germany). Brain slices containing hippocampal areas of all three animals were deposited on the same slide ...
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bioRxiv - Neuroscience 2022Quote: ... and sliced at 16 μm sections with a cryostat (Leica CM1950 ...
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bioRxiv - Neuroscience 2021Quote: ... Twelve-16 µm sections were cut using a cryostat (Leica) and collected on charged glass microscope slides (SuperFrost Plus ...
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bioRxiv - Neuroscience 2023Quote: ... Thin sections (16 μm) were cut on a cryostat (Leica) and processed for antibody staining [17] ...
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bioRxiv - Neuroscience 2023Quote: ... 16-µm-thick brain sections were cut with cryotome (Leica). After rehydration in 1× PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... Antigen retrieval was performed in BOND Epitope Retrieval Solution 1 (pH 6) or 2 (pH 9) (ER1, ER2, Leica Biosystems, Cat. AR9961, AR9640) at 95°C ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were then cryoprotected for 2h in 20% sucrose in PBS at 4°C and sectioned at 20 µm or 40-45 µm thickness with a sliding microtome (Leica). The tissues were then processed in function of the target immunofluorescence as described below.
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bioRxiv - Developmental Biology 2019Quote: ... The samples were embedded in gelatin/ albumin with 4% of glutaraldehyde and sectioned (20 μm) on a VT1000 S vibrating blade microtome (Leica). Sections were mounted in Fluoromount Aqueous Monting Medium (Sigma).
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bioRxiv - Developmental Biology 2019Quote: ... EBs were fixed in 4% paraformaldehyde for 7-8 hours at 4°C followed by equilibration in 20% sucrose solution overnight and embedded in tissue freezing medium (Leica). The embedded tissues were then cryo-sectioned and collected on Superfrost slides (Fisher Scientific) ...
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THE OLFACTORY RECEPTOR Olfr78 REGULATES DIFFERENTIATION OF ENTEROCHROMAFFIN CELLS IN THE MOUSE COLONbioRxiv - Cell Biology 2023Quote: ... overnight at +4°C and then sedimented through 20% and 30% sucrose solution sequentially (minimum 24 hours each) before OCT (Leica) embedding ...
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bioRxiv - Microbiology 2021Quote: ... Antigen retrieval was performed by epitope retrieval (ER) 2 solution (Leica, pH 9). The slides were then incubated with anti-E-cadherin ...
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bioRxiv - Cancer Biology 2020Quote: ... diluted in Novolink DAB Substrate Buffer (Leica Biosystems, 1:20, 5 min). Finally ...
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bioRxiv - Cell Biology 2021Quote: ... Slides were retrieved for 20□min using Epitope Retrieval 1 (Citrate; Leica) and incubated in Protein Block (Dako ...
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bioRxiv - Immunology 2023Quote: ... Slides were retrieved for 20 min using Epitope Retrieval 1 (Citrate; Leica) and incubated in Protein Block (Dako ...
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bioRxiv - Immunology 2022Quote: ... 3′-3-diaminobenzidine (DAB) substrate (Leica Microsystems) was used as a chromogen followed by hematoxylin counterstain ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were embedded in agar (3-4%) and coronal sections (50 µm) were cut on a vibrating microtome (Leica VT1000S) and collected in a 1 in 4 series ...
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bioRxiv - Cell Biology 2021Quote: ... Dried samples were then coated with 3 nm platinum and 4 nm carbon using an ACE600 coating device (Leica Microsystems). The sample grids were imaged using a JEOL JEM2800 scanning/transmission electron microscope at 200 kV.
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bioRxiv - Cell Biology 2020Quote: ... Bright field images of beating cilia were captured at 160 frames per sec over 4 seconds (3-5 videos per insert, Leica Spinning disc confocal with 40x water objective) ...
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bioRxiv - Plant Biology 2022Quote: ... The samples were then coated with 3 nm platinum and 4 nm carbon using the ACE600 coating device (Leica Microsystems).
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bioRxiv - Plant Biology 2023Quote: ... they were kept at 28±1°C in dark for 16 h and observed under confocal microscope (Leica microsystems, TCS, SP5). BiFC for each combination was repeated twice and appropriate negative controls were used (Kudla and Bock ...
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bioRxiv - Cancer Biology 2021Quote: ... endogenous peroxidase activity was blocked by incubation with 3% peroxide block for 20 minutes (BOND Polymer Refine Detection System, Leica, Germany; DS9800). The sections were blocked with 10% normal goat serum (Vector ...
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bioRxiv - Neuroscience 2020Quote: ... the brain of each mouse extending from anterior to the DG to the cerebellum (from 0.22 to −5.34 μm from Bregma) was sectioned at 30 μm in a 1:9 series using a freezing microtome (Leica SM 2000 R Sliding Microtome). Sections were stored in 1xPBS with 0.01% sodium azide at 4°C until processing for IHC ...
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bioRxiv - Developmental Biology 2021Quote: ... EBs or brain tissues were fixed in 4% paraformaldehyde for 7-8 hours at 4°C followed by equilibration in 20% sucrose solution overnight and embedded in tissue freezing medium (Leica, 14020108926). The embedded tissues were then cryo-sectioned and collected on Superfrost slides (Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were post fixed in 4% paraformaldehyde overnight and cryoprotected in 20% sucrose azide prior to freezing and sectioning at 40μm (CM3050S; Leica, Wetzlar, Germany). Tissue slices containing M2 were stained with neutral red and electrode placement was validated ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were sliced at 16 mm thickness using a cryostat (Leica) and every fourth section was thaw-mounted onto slides ...