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Citations for Leica :
1 - 50 of 1729 citations for 6β Fluoro 21 hydroxypregna 1 4 9 11 16 tetraene 3 20 dione 21 acetate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... heads were sectioned into 20 µm slices (at -21 °C) using a Cryotome CM1900 (Leica) and mounted on Superfrost Plus slides (Thermo Fisher Scientific).
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bioRxiv - Developmental Biology 2021Quote: ... the heads were sectioned into 20 μm slices (at −21°C) using a cryotome CM1900 (Leica) and mounted on Superfrost Plus slides (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2020Quote: ... Neurons were transduced with LV-Syn1-GFP at DIV 3 and were imaged at DIV 21 using an inverted spinning disk confocal microscope (Leica DMi8, 63x objective). During image recording the cells were maintained in a humidified atmosphere of 5% CO2 at 37°C ...
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bioRxiv - Plant Biology 2022Quote: ... 21 dpi and were frozen in a high-pressure freezer (EM-ICE; Leica, Vienna, Austria) by using the fixation solution (0.25% (w/v ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were then sliced into 55 μm sections at −21°C using a cryostat (Leica).
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bioRxiv - Microbiology 2022Quote: ... Grids were post-stained for 30 min with 2% uranyl acetate (Roth) and 20 min with 3% lead citrate (Leica). TEM images were acquired using a Zeiss Leo 912 Omega (Zeiss ...
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bioRxiv - Developmental Biology 2021Quote: ... a z-stack (1 µm sections) was acquired at 21–22 °C with the HC PL APO 63×/1.40 NA oil immersion objective (Leica).
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bioRxiv - Zoology 2021Quote: ... The sections were deparaffinised and stained with haematoxylin and eosin [21](McMannus and Mowry, 1965) in auto-stainer (Leica, Germany) and cover slipped by auto cover slipper (Leica ...
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bioRxiv - Plant Biology 2019Quote: ... were mounted on slides in ½ MS liquid media (0% sucrose, pH 5.7, 21°C) and imaged using a TCS-SP8 microscope (Leica, Germany) equipped with a 10X/0.3 NA or 63X/1.2 NA water immersion objective ...
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bioRxiv - Neuroscience 2019Quote: ... for 3 min and uranyl acetate (Leica Ultrostain I, Leica Microsystems) for 10 min using an automatic contrasting system (Leica EM AC20 ...
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bioRxiv - Neuroscience 2019Quote: ... for 3 min and uranyl acetate (Leica Ultrostain I, Leica Microsystems) for 10 min using an automatic contrasting system (Leica EM AC20 ...
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bioRxiv - Neuroscience 2020Quote: ... Motoneurons were patched at room temperature (approx 21°C) using infrared-differential interference contrast (IR-DIC) optics on a DMLFSA microscope (Leica DMLFSA; Leica Microsystems). All lumbar motoneurons were selected from the lateral motor nuclei of segments L1-6 and all cervical cells were selected from the lateral motor nuclei of slices made from C4-8 ...
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bioRxiv - Genetics 2022Quote: Visual screening: Visual fluorescence screening of 14–21-day old F0 embryos on a Fluorescent Stereo Microscope (Leica M165FC; Figure 1B) was conducted to verify successful knock-in of cDNA encoding fluorescent proteins ...
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Computational toolbox for ultrastructural quantitative analysis of filament networks in cryo-ET databioRxiv - Cell Biology 2021Quote: ... and vitrification using a Leica GP2 plunger equipped with a blotting detection sensor and incubation chamber maintaining an environment of 21°C and 90% humidity (Leica Microsystems). Grids were placed into the GP2 incubation chamber and excessive liquid was manually removed with a piece of filter paper by gently touching the side of the grid ...
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bioRxiv - Biochemistry 2021Quote: ... Mouse brain tissue sections were sectioned at −21°C to a 12 μm thickness using a CM1850 Cryostat (Leica Microsystems, Wetzlar, Germany). Tissue sections were thaw mounted onto glass microscope slides (IMEB ...
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bioRxiv - Cell Biology 2020Quote: Sample blocks were obtained by freeze substitution of the cell pellets in 0.5 % uranyl acetate diluted from a 20 % stock in methanol and 3 % double distilled H2O in acetone in a Leica AFS1 (Leica Microsystems GmbH, Wetzlar, Germany). Temperature was set to −130 °C for the first 2 hours and then raised to −90 °C with a 20 °C/hr gradient ...
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bioRxiv - Neuroscience 2020Quote: ... Motoneurons were patched at room temperature (approx 21°C) using infrared-differential interference contrast (IR-DIC) optics on a DMLFSA microscope (Leica DMLFSA; Leica Microsystems). All lumbar motoneurons were selected from the lateral motor nuclei of segments L1-6 and all cervical cells were selected from the lateral motor nuclei of slices made from C4-8 ...
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bioRxiv - Neuroscience 2019Quote: ... neurons were imaged every 20 minutes for 16 hours by Leica DMI6000 equipped with a heating box and CO2 atmosphere.
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bioRxiv - Developmental Biology 2023Quote: ... Tissue slices of 16–20 mm were cut in a cryostat (Leica CM1850). Anaesthesised adult mice were processed by cardiac perfusion and their brains processed was described (Nasif et al ...
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bioRxiv - Microbiology 2023Quote: ... antigen retrieval (20 minutes at 100°C in Leica Bond-Epitope Retrieval Solution 2 pH-9) was performed on dewaxed tissues prior to labeling with CD4 antibody (ab183685 ...
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bioRxiv - Bioengineering 2023Quote: ... and 20 min in 3% lead citrate (Leica, Germany). TEM images were acquired with a 200 kV TEM JEM 2100Plus (Jeol ...
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bioRxiv - Developmental Biology 2019Quote: ... Cell counts were done on serial sections immunostained for GnRH-1 at E13.5 (n=3) and E15.0 (n=4) and visualized under bright field (immunoperoxidase) or epi-fluorescence illumination (20×; Leica DM4000 B LED), according to their anatomical location [i.e. ...
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bioRxiv - Neuroscience 2023Quote: ... Transverse sections at 16 μm were cut at -20 °C using a cryostat (CM3050-S, Leica) and mounted directly on Superfrost Plus slides (Cat ...
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bioRxiv - Cell Biology 2024Quote: ... sectioned to 3-4 μm thickness (Leica RM2255, Germany), and stained with hematoxylin and eosin (H&E) ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were freeze substituted in acetone containing 0.1% uranyl acetate plus 3% distilled water in a freeze substitution unit (Leica EM AFS2 ...
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bioRxiv - Bioengineering 2023Quote: ... were afterwards stained for 30 min in 1% aqueous uranyl acetate (Leica, Germany) and 20 min in 3% lead citrate (Leica ...
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bioRxiv - Cell Biology 2023Quote: ... Blocks were stored at - 80°C before cutting into 16-20 µm sections using a cryostat (Leica CM3050S). Cryosections were collected on Superfrost Plus slides (Thermo Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... Antigen retrieval was carried out with TRIS buffer at pH 9 for 20 mins at 95°C using Bond Epitope Retrieval Solution 2 (Leica Biosystems). HA and Myc tag wer detected using anti-HA (see above ...
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bioRxiv - Cell Biology 2021Quote: ... freeze-substituted in acetone / 1% OsO4 / 5% H2O / 0.25% uranyl acetate (AFS2, Leica, Wetzlar, Germany) and embedded in Epon ...
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bioRxiv - Neuroscience 2020Quote: ... containing dorsal hippocampus (from 3, 6, 9, 12, 15, 19, and 24 month-old) were cut from PFA-fixed hemispheres (Leica vibratome VT1000P) and stored in 0.1M phosphate-buffered saline (PBS ...
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bioRxiv - Cell Biology 2019Quote: PFA-fixed adult mouse (3-4 month) brains were cut into 1-mm-thick sections using a vibratome (Leica, VT1200S) [11 ...
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bioRxiv - Neuroscience 2019Quote: ... 3 and 4 are compositions of more fields automatically generated by Leica LAS X software or NIS elements software ...
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bioRxiv - Pathology 2021Quote: ... Slides were then treated with 3-4% hydrogen peroxide (Leica Biosystems RE7101) for 10 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were freeze substituted in acetone containing 0.1% uranyl acetate plus 3% distilled water in a freeze substitution unit (Leica EM AFS2, Leica Microsystems, Buffalo Grove, IL) at −90°C for 20 hours ...
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bioRxiv - Neuroscience 2023Quote: ... were rinsed in PBS and embedded in 3% agarose containing 1% Tween 20 and sectioned at 300 µm in the frontal plane with a vibratome (Leica VT 1200S). Under a stereomicroscope (Olympus SZX7) ...
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bioRxiv - Cell Biology 2023Quote: ... and blotted for 9 seconds by Leica GP2 (Leica Microsystems) ...
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bioRxiv - Neuroscience 2023Quote: ... 11 mM glucose using a vibratome (Leica VT1200). Slices were then transferred to artificial cerebral spinal fluid (aCSF ...
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bioRxiv - Microbiology 2024Quote: ... embedded in paraffin and sectioned (4 μm) at −20°C (RM2016, Leica, Germany).
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bioRxiv - Cell Biology 2024Quote: ... 16 μm cryostat (Leica CM3050S) sections were thaw-mounted on gel-coated slides and stored at 20°C until use ...
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bioRxiv - Cell Biology 2023Quote: ... specimens were sputter coated with 3-4 nm of platinum (Leica EM ACE600). Samples were imaged using a Helios Nanolab 660 DualBeam Microscope (FEI).
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bioRxiv - Molecular Biology 2022Quote: ... temporal bones were immersed in 30% sucrose in PBS and stored at 4°C for 7 days before being cut into 14-16 micron thick sections on a cryostat (Leica CM3050). For each species ...
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bioRxiv - Neuroscience 2023Quote: ... and 11 mM glucose) using a vibratome (VT1000S, Leica). Slices were recovered in a holding chamber containing oxygenated (95% O2 and 5% CO2 ...
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bioRxiv - Neuroscience 2020Quote: ... post-fixed in 4% PFA for 24 hours at 4°C and sectioned (20-60 μm) using a vibratome (Leica VT 1000s). If necessary ...
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bioRxiv - Bioengineering 2023Quote: Primary Antibodies: anti-Dystrophin (Leica, NCLDYS1, 1:20), recombinant Anti-Lamin A + Lamin C antibody (Abcam ...
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bioRxiv - Immunology 2022Quote: ... and CD163 (Leica Microsystems, 10D6, 1:20 dilution). Subsequent visualization was performed using Akoya Opal fluorophores (620 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... About 3-4 µm sections were prepared using an automated microtome (RM2255, Leica Biosystems, Germany) and the sections were stained with hematoxylin and eosin (H&E ...
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bioRxiv - Microbiology 2022Quote: ... sapphire discs placed in adapted carriers were filled with freeze substituent (0.1% uranyl acetate in acetone, 1% H2O) and placed in a temperature-controlling AFS2 (Leica) equipped with an FPS robot ...
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bioRxiv - Cell Biology 2023Quote: ... Vitrified samples were freeze-substituted in 1% osmium tetroxide/0.1% uranyl acetate in acetone (AFS2, Leica Microsystems, Vienna, Austria), and processed to Epon-Araldite resin as previously described.39 Tissue samples were stained with 1% osmium tetroxide ...
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bioRxiv - Immunology 2023Quote: ... and stained with uranyl acetate and lead citrate (Leica AC20). Micrographs were collected on a JEM1010 (JEOL ...
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bioRxiv - Developmental Biology 2020Quote: ... Image stacks were obtained every 3-4 minutes for 6-8 hours using a LSM880 (Leica) with an environmental chamber (37°C with 5% CO2) ...