Labshake search
Citations for Leica :
401 - 450 of 1579 citations for 6 Oxa 7 azatricyclo 3.3.0.02 4 oct 7 ene 8 1 methylethyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2022Quote: In vivo images of mouse retinal structure were obtained with spectral domain-optical coherence tomography (SD-OCT) (Leica Microsystems, Buffalo Grove, IL). The mice received 1% tropicamide drops to stimulate eye dilation and were anesthetized with ketamine and xylazine (50 and 5 mg/kg body weight ...
-
bioRxiv - Neuroscience 2019Quote: ... the foil was removed and the frozen OCT block was mounted slice-side up on a specimen stub with OCT in a precooled (specimen holder, −18°C; chamber, −18°C) cryostat (Leica CM3050 S). Once the temperature of the embedded slice had equilibrated ...
-
bioRxiv - Neuroscience 2021Quote: ... Cryosectioning was performed by embedding brains in optimal cutting temperature compound (OCT) and cutting serial sections on a rotary cryostat (Leica CM1850, Leica Biosystems GmBH). 5-10μm coronal sections containing the ventral hippocampus were collected on Super Frost Ultra Plus slides (Fisher Scientific ...
-
bioRxiv - Neuroscience 2022Quote: ... the tissues were embedded in Optimal Cutting Temperature compound (OCT, Sakura) and sectioned into 30-μm slices using a cryostat (Leica CM3050 S). Frozen tissues stored at −80°C ...
-
bioRxiv - Physiology 2021Quote: ... Diaphragm bundles were processed for immunohistochemical analyses of fiber cross-sectional area and myosin heavy chain isoforms as described previously.57,61 Bundles embedded in Tissue-Tek OCT freezing medium were sliced into sections of 10 μm thickness at approximately -20ºC using a cryostat (Leica, CM 3050S model). Sections were incubated in 1:200 wheat germ agglutinin (WGA ...
-
bioRxiv - Cancer Biology 2021Quote: ... The prefrozen liver tissues in OCT were transverse sectioned at a thickness of 10 µm using a CM1950 cryostat (Leica, Wetzlar, Germany). Tissue sections adhering to the Stereo-seq chip surface were incubated at 37°C for 3 min ...
-
bioRxiv - Neuroscience 2022Quote: ... Tissues were covered with Optimal Cutting Temperature (OCT) medium (CellPath, Newtown, UK) and sections were obtained at 30 μm on a Leica SM2010R Freezing Microtome (Leica, Wetzlar, Germany). All sections were subjected to heat-mediated antigen retrieval in 10 mM sodium citrate (pH6.5 ...
-
bioRxiv - Molecular Biology 2023Quote: ... Tissue was stored in −80 °C and sectioned from an OCT block at 12 µm thickness and mounted on Leica PPS-membrane Laser Microdissection (LMD) slides (Leica, Cat# 11505268). A rapid stain protocol23 prepared slides for LMD ...
-
bioRxiv - Neuroscience 2023Quote: ... embedded in OCT mounting medium and cut into 12 μm thick transverse sections with a cryostat (Leica CM1950; Leica; Milton Keynes, UK). These were mounted in Prolong-Glass anti-fade medium with NucBlue (P36981 ...
-
bioRxiv - Neuroscience 2023Quote: ... embedded in OCT mounting medium and cut into 12 μm thick transverse sections with a cryostat (Leica CM1950; Leica; Milton Keynes, UK). These were mounted in Prolong-Glass anti-fade medium with NucBlue (P36981 ...
-
bioRxiv - Bioengineering 2023Quote: ... The postfixed brains were frozen in Tissue Tek OCT at −80 °C and sliced coronally into 20 μm sections with a cryostat machine (CM1900, Leica Microsystems, USA). The sections were washed with 0.1 m PBS three times for 15 min each and blocked for 1 hour with a solution of 10% normal goat serum (Vector Laboratories ...
-
bioRxiv - Neuroscience 2023Quote: ... Tissues were embedded in OCT and frozen on dry ice and stored at -80°C until sectioning on a cryostat (Leica CM3050-S). Brain sections were mounted at 10 μm thickness on Superfrost Plus slides (1255015 ...
-
bioRxiv - Neuroscience 2023Quote: ... Cryosectioning was performed by embedding brains in optimal cutting temperature compound (OCT) and cutting serial sections on a rotary cryostat (Leica CM1950, Leica Biosystems GmBH). 20 μm coronal sections containing the ventral hippocampus were collected on Super Frost Ultra Plus slides (Fisher Scientific ...
-
bioRxiv - Neuroscience 2023Quote: ... embedded in Tissue-Tek OCT compound (Sakura Finetek, Torrance, CA, US) and cryosectioned (20 μm of thickness; CM 1860 Cryostat; Leica, Wetzlar, Germany). Antibody staining was carried out at room temperature using standard protocols ...
-
bioRxiv - Cell Biology 2023Quote: ... USA) and Bioptigen Envisu R2200 ultra-high resolution (UHR) spectral-domain optical coherence tomography system (SD-OCT; Leica Microsystems, Deerfield, IL, USA) (22) ...
-
bioRxiv - Molecular Biology 2024Quote: ... the brain tissue was embedded in OCT(4583, SAKURA, Japan) and coronal sections were made in a freezing microtome (LEICA CM1950, Germany) at −20°C with a thickness of 20µm ...
-
bioRxiv - Plant Biology 2022Quote: ... Fixed and stained roots were mounted onto microscopic slides and cleared with chloral hydrate:glycerol:water (8:3:1 ratio) before imaging with the Thunder Imaging Systems Microscope (Leica).
-
bioRxiv - Neuroscience 2021Quote: ... The brain tissue was then embedded in OCT medium and sliced to 14 μm thick sections on a cryostat (Leica HM 505 E). Tissue sections were collected directly onto SuperFrostPlus slides and stored at −80 deg for up to 3 months until RNA in situ hybridization for EYFP (virally driven) ...
-
bioRxiv - Neuroscience 2022Quote: ... Cryoprotected spinal cords were embedded in optimal cutting temperature (OCT) compound and frozen at −80 °C until cryostat sectioning (Leica Lm 1850, Germany). Transverse lumbar spinal cord cryosections were collected in serial at 30-μm or 40-μm thickness ...
-
bioRxiv - Plant Biology 2021Quote: Frozen OCT moulds containing fresh leaves were moved from -80 °C to -20 °C inside a cryomicrotome (CM 3050S, Leica Biosystems; MA, US) to acclimate for 30 min ...
-
bioRxiv - Neuroscience 2020Quote: ... Fresh frozen lumbar spinal cord segments were embedded in OCT mounting medium then cut into 12[μm-thick transverse sections on a cryostat (Leica CM1860; Leica; Milton Keynes, UK) and mounted on SuperFrost Plus slides (48311[703 ...
-
bioRxiv - Neuroscience 2022Quote: ... Brains were cryopreserved and embedded in optimal cutting temperature compound (OCT) followed by coronal sectioning (12 μm) on a cryostat (Leica Microsystems, Exton, PA). The third ventricle was used as a regional anatomical marker for the region of the corpus callosum most consistently demyelinated by cuprizone ...
-
bioRxiv - Neuroscience 2023Quote: ... Lumbar segments were then embedded in OCT mounting medium and 12 µm-thick transverse sections were cut on a Leica CM 1950 cryostat (Leica, Milton Keynes, UK). Sections were mounted non-sequentially (such that sections on the same slide were at least 4 apart ...
-
bioRxiv - Cell Biology 2024Quote: ... followed by an overnight incubation with mild shaking in a mixture of equal parts 34% sucrose in 1xPBS and optimal cutting temperature compound (OCT; Leica Biosystems Cat.#14020108926) liquid at 4°C ...
-
bioRxiv - Neuroscience 2023Quote: ... All brains were then flash frozen in OCT solution and cut at 100um thickness using a cryostat (Leica CM 1950) (Leica Biosystems, Illinois/USA). Pyramidal neurons in dorsal CA1 region of the hippocampus ...
-
bioRxiv - Neuroscience 2024Quote: ... The brains were frozen in Optimal Cutting Temperature (OCT) embedding media and 16 μm (for FISH) or 35 μm (for IF) coronal sections were cut with vibratome (Leica, no. CM3050 S). For FISH experiments ...
-
bioRxiv - Cancer Biology 2022Quote: ... or confocal microscope (Leica SP-8, Wetzlar, Germany) at 63X magnification.
-
bioRxiv - Plant Biology 2022Quote: ... a Leica SP8 or Stellaris 8 (Leica, Germany) confocal microscope was used ...
-
bioRxiv - Plant Biology 2023Quote: ... A laser scanning confocal microscope (Leica SP-8) was used to examine and capture the fluorescence emitted by fusion proteins 72 hours after infiltration ...
-
bioRxiv - Physiology 2019Quote: ... Ultra-thin sections of 50-60□nm thick were cut by Leica EM UC 6 (Leica Microsystems, Germany) and contrasted with uranyl acetate ...
-
bioRxiv - Physiology 2020Quote: ... The eyes were placed under the sample arm of an SD-OCT equipped with a 10 mm telecentric lens (Envisu R2210, Leica, Bioptigen, Morrisville, NC, USA). The scanning beam was oriented perpendicularly to a portion of the limbus in which intrascleral signal voids of the outflow tract could be visualized ...
-
bioRxiv - Neuroscience 2021Quote: ... Cryosectioning was performed by embedding brains in optimal cutting temperature compound (OCT) and cutting serial sections on a rotary cryostat (Leica CM1850, Leica Biosystems GmBH). 5-10μm coronal sections containing the ventral hippocampus were collected on Super Frost Ultra Plus slides (Fisher Scientific ...
-
bioRxiv - Neuroscience 2020Quote: ... OCT-embedded brains were sectioned (35 µm) throughout on a horizontal (axial) plane with a cryostat (Leica CM3050S, Leica Biosystems, Buffalo Grove, IL, USA), and collected in individual wells ...
-
Cbln1 directs axon targeting by corticospinal neurons specifically toward thoraco-lumbar spinal cordbioRxiv - Neuroscience 2022Quote: ... brains or spinal cords were placed in Tissue-Tek OCT Compound (Sakura Finetek, Torrance, CA) for sectioning using a cryostat (Leica CM3050 S, Wetzlar, Germany). Prior to sectioning ...
-
bioRxiv - Neuroscience 2023Quote: ... Cryosectioning was performed by embedding brains in optimal cutting temperature compound (OCT) and cutting serial sections on a rotary cryostat (Leica CM1950, Leica Biosystems GmBH). 20 μm coronal sections containing the ventral hippocampus were collected on Super Frost Ultra Plus slides (Fisher Scientific ...
-
bioRxiv - Cell Biology 2020Quote: ... Tissues were fixed with 4% PFA for 1 h prior to vibratome (Leica) sectioning at 100 μm ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... embryos were embedded and sectioned (8 μm) by Leica CM3050S.
-
bioRxiv - Neuroscience 2020Quote: ... Fluorescence & FISH Digital Pathology Scanner (Leica, Aperio VERSA 8). For three-dimensional reconstruction of injected or virus labelled RGCs ...
-
bioRxiv - Cell Biology 2021Quote: ... PMNs were examined on a Stellaris 8 (Leica Microsystems) with an oil 63× oil magnification lens using LAS X software 3.7.3.23245 (Leica Microsystems).
-
bioRxiv - Neuroscience 2022Quote: ... sectioned coronally at 8 μm thickness (Leica CM 3050S) on Superfrost slides (Thermo Fisher Cat# 22-037-246 ...
-
bioRxiv - Bioengineering 2023Quote: ... we used an inverted light microscope (Leica Dmi-8) with a 10× objective lens (HC PL APO CS2 10x/0.40 DRY ...
-
bioRxiv - Developmental Biology 2023Quote: ... and sectioned (8 μm) using a CM1950 Cryostat (Leica). For 1 week-old samples ...
-
bioRxiv - Neuroscience 2019Quote: Brains were mounted in OCT embedding media (Bright) and cut into 14 µm sections across the coronal plane using a cryostat (Leica CM 1860 UV, Ag Protect) and collected on SuperFrost Plus microscope slides (Thermo Scientific) ...
-
bioRxiv - Neuroscience 2020Quote: ... Fresh frozen lumbar spinal cord segments were embedded in OCT mounting medium then cut into 12[μm-thick transverse sections on a cryostat (Leica CM1860; Leica; Milton Keynes, UK) and mounted on SuperFrost Plus slides (48311[703 ...
-
bioRxiv - Neuroscience 2022Quote: ... after which the samples were embedded in OCT (Tissue Tek, Sakura, Germany. 40 micron sections were cut with a cryostat (Leica CM 1950 AG Protect cryostat). Sections spanning the injury site were selected and blocked (3% BSA ...
-
bioRxiv - Molecular Biology 2022Quote: Muscle samples that were collected and preserved in OCT as described above were sectioned at a thickness of 10 μm in a cooled (−22°C) cryostat (Leica Biosystems; Buffalo Grove, IL, USA) and electrostatically adhered to positively charged histology slides ...
-
bioRxiv - Neuroscience 2023Quote: ... Brains were sectioned at 50µm (1 in 6 series) in the coronal plane with a vibratome (model VT1000, Leica). Sections were stored at -20°C in a cryoprotectant solution comprised of 60% glycerol and 0.01% sodium azide in 0.1M PBS.
-
bioRxiv - Cancer Biology 2021Quote: ... Slides were scanned on a VERSA 8 slide scanner (Leica) prior to analysis in ImageScope v64 and ImageJ.
-
bioRxiv - Developmental Biology 2021Quote: ... Cryosections were cut to 8 µm on a cryostat (Leica) and mounted on SuperFrost Plus slides (Fisher).
-
bioRxiv - Developmental Biology 2019Quote: ... Sections (8 μm) were prepared with a microtome (RM2235, Leica), stained with 0.5% toluidine blue and observed with a microscope (BX53 ...