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Citations for Leica :
301 - 350 of 1579 citations for 6 Oxa 7 azatricyclo 3.3.0.02 4 oct 7 ene 8 1 methylethyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Frozen brains were cut to either 10 μm slices for immunofluorescence staining or 4-6 μm for STORM (CM1950, Leica) to produce coronal brain sections.
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bioRxiv - Neuroscience 2021Quote: ... Brains were embedded and frozen in OCT (TissueTEK) and sectioned as 30 μm floating sections by cryostat (Leica). Free-floating coronal sections were stored at −20°C in antifreeze solution until use ...
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bioRxiv - Immunology 2021Quote: Unfixed pediatric tonsil samples frozen in OCT medium were cut into 9μm thick sections using a cryostat (Leica) and stored at −80°C ...
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bioRxiv - Pathology 2019Quote: ... Tissue was mounted in OCT and cooled to −20°C for sectioning by a cryostat (Leica Biosystems, USA). Sections of 10 – 20 µm were mounted onto Frost + microscope slides and stored at −20°C.
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bioRxiv - Cancer Biology 2019Quote: ... The OCT-mounted iWAT and BAT blocks were sliced at 14μm using a Cryostat (Leica CM3050S, Nussloch, Germany) at −40°C ...
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bioRxiv - Neuroscience 2020Quote: ... Tissues were cryoprotected overnight in 25% sucrose before being embedded in OCT and cut on a cryostat (Leica CM3050S or Thermo Fisher Scientific CryoStar NX50).
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bioRxiv - Neuroscience 2020Quote: ... embedded in OCT medium and sectioned at a thickness of 12-14 µm on a cryostat (CM1800, Leica). The sections were washed in PBS and pre-blocked in blocking solution - 5% goat serum ...
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bioRxiv - Developmental Biology 2021Quote: ... They were then frozen in Tissue-Tek OCT (Sakura) at −80°C and sectioned using a Cryostat (Leica). Zebrafish larvae sections were incubated in blocking buffer (10% serum in PBST ...
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bioRxiv - Cancer Biology 2022Quote: ... Japan) and frozen samples embedded in OCT compound were sectioned at a thickness of 10 μm (Leica CM3050S). We utilized the 10x Genomics Visium Spatial Gene Expression Solution for spatial transcriptomics ...
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bioRxiv - Neuroscience 2022Quote: ... These were cryoprotected in 30% sucrose and then embedded in OCT (Optimal Cutting Temperature Tissue-Tek compound, Leica). Frozen sections were cryosectioned at 10 μm and processed for immunohistochemistry using the streptavidin horseradish peroxidase complex ...
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bioRxiv - Plant Biology 2022Quote: ... the OCT-blocks with root samples were transferred to a pre-cooled (25°C) cryotome (Leica model CM050S) and were left to equilibrate for 1 hour ...
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bioRxiv - Developmental Biology 2023Quote: ... digit tissue was dehydrated in a sucrose gradient up to 30% sucrose/PBS and cryopreserved in OCT (Leica). Cryosectioning was performed at 18 μm thickness.
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bioRxiv - Neuroscience 2023Quote: ... Frozen samples were embedded in OCT (TissueTek Sakura, Cat# 4583) and cryosectioned at 10 micron thickness (Leica, CM3050s). Two to three non-adjacent tissue sections from the same ITC block were mounted on a Visium Spatial Gene Expression Slide (10x Genomics ...
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bioRxiv - Cancer Biology 2024Quote: ... or frozen at –80 °C in optimal cutting temperature gel (OCT) for cryosectioning at 16 μm (Leica CM3050S). Permeabilization and blocking were done in PBS with 5% normal goat serum (NGS ...
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bioRxiv - Neuroscience 2024Quote: ... brains were embedded in OCT (Tissue-Tek) and cut into 30-µm-thick sections on cryostat microtome (Leica). Sections were permeabilized with 0.3% Triton X-100 and 5% BSA in PBS for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... for stepwise incubation with BOND Epitope Retrieval Solution 1 pH 6 (AR9961, Leica) at 100°C for 20 min ...
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bioRxiv - Neuroscience 2023Quote: ... Antigen retrieval was done using Epitope retrieval buffer 1 (pH 6; Leica biosystems) for 20 minutes ...
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bioRxiv - Microbiology 2020Quote: Total cell counts were determined by staining with 4’,6-diamidino-2-phenylindole (DAPI) and visualizing on a Leica DMRX epifluorescence microscope (Leica, Germany). The number of FLA-YM stained cells were determined by enumerating cells which had a positive DAPI and FLA-YM signal (excitation at 405 nm and 488 nm wavelengths ...
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bioRxiv - Neuroscience 2022Quote: ... and embedded in OCT (BDH) before freezing and sectioning (16µm thickness) in a sagittal plane with a cryostat microtome (Leica).
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bioRxiv - Neuroscience 2022Quote: ... Brains were embedded in OCT medium (Tissue-Tek; Sakura, Torrance, CA) and serially sectioned by cryostat (Leica, Wetzlar, Germany) into 40-μm coronal slices containing the LC and PFC ...
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bioRxiv - Molecular Biology 2022Quote: ... Cross-sectional images were generated using 1000 rectangular volume scans using the Envisu OCT system (Leica Microsystems, Wetzlar, Germany). Outer nuclear layer and inner nuclear layer thickness was measured using the linear caliper function in the software by a masked observer using a pre-established uniform grid.
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bioRxiv - Microbiology 2022Quote: Organ samples frozen in OCT were cut into 10 µm sections using a CM1850UV cryostat (Leica Biosystems, Wetzlar, Germany) and adhered to polysine microscopy slides (Thermo Scientific) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... samples were embedded in OCT cryosection medium and cryosectioning was performed using either a CM3050S or CM1950 cryostat (Leica). HCR probes (version3 chemistry ...
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bioRxiv - Neuroscience 2023Quote: ... tissue was immersed in 30% sucrose for 2 days followed by embedding in Tissue-Tek OCT and sectioning of 50μm slices in a cryostat (Leica). Staining was done as described (Licht et al. ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were transferred to 30% sucrose in 1x PBS for 72 hours and then embedded in OCT compound (Leica). Brain sections (40 μm ...
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bioRxiv - Neuroscience 2022Quote: VNOs embedded in OCT-medium were sliced in 16 µm thick sections in a cryostat apparatus (Leica CM 3050S). Slices were incubated in blocking solution containing 0.5 % Triton X-100 and 5 % normal horse serum in 0.1 M TBS for 2 h at room temperature (RT) ...
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bioRxiv - Neuroscience 2023Quote: ... The brains were then embedded in OCT and preserved at –80°C until cryosectioning at 60 µm (Leica CM3050). For free-floating immunostaining ...
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bioRxiv - Neuroscience 2023Quote: ... submerged in OCT medium, flash frozen, and then sliced (50μm, parasagittal plane) using a cryostat microtome (CM 3050S, Leica).
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bioRxiv - Molecular Biology 2022Quote: ... Green siliques were harvested 12 d after flowering from 4-6 week old plants and opened using fine needles under a dissecting microscope (Leica MZ9.5, Germany). Images of opened siliques were captured using a Leica DFC290 (Germany ...
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bioRxiv - Cell Biology 2022Quote: ... Dried samples were then rotary shadowed with 2 nm of platinum and 4-6 nm of carbon using the ACE600 (Leica Microsystems, Germany). Areas of interest from the resulting platinum replicas were separated from glass with 5% hydrofluoric acid and deposited on carbon-coated EM grids (200 mesh formvar/carbon ...
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bioRxiv - Cell Biology 2022Quote: ... Dried samples were then rotary shadowed with 2 nm of platinum (sputtering) and 4-6 nm of carbon (carbon thread evaporation) using an ACE600 metal coater (Leica Microsystems, Germany). The resultant platinum replica was floated off the glass with hydrofluoric acid (5%) ...
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bioRxiv - Neuroscience 2023Quote: ... Images of cultured ependymocytes co-treated with Sir-Actin and calcein AM were captured at 10-s intervals for 12 minutes while that of co-treated with Sir-Actin and fluo-4 AM were captured at 10-s intervals for 6 minutes using confocal scanning microscope (DMi8, Leica, Wetzlar, Germany). The intensity of F-actin area from all Sir-Actin positive cells in a field of recording were included in the statistics ...
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bioRxiv - Neuroscience 2023Quote: ... The fluorescence signals of Fluo-4 were monitored at 10-s intervals for 6 minutes by confocal scanning microscope (DMi8, Leica, Wetzlar, Germany). As previously described 46 ...
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bioRxiv - Microbiology 2024Quote: ... containing 4‘,6-diamidino-2-phenylindole (DAPI) and imaged as midsections by confocal laser scanning microscopy (Leica SP8: Leica Microsystems, Wetzlar, Germany). To prevent cross talk between the channels for the different fluorochromes ...
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bioRxiv - Cell Biology 2023Quote: ... Dried samples were then rotary shadowed with 2 nm of platinum (sputtering) and 4-6 nm of carbon (carbon thread evaporation) using an ACE600 metal coater (Leica Microsystems, Germany). The resultant platinum replica was floated off the glass with hydrofluoric acid (5%) ...
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bioRxiv - Molecular Biology 2021Quote: ... embedded in OCT tissue freezing medium and 5 μm cross-sectioned on a Cryostat (Leica CM3050 S, Leica Biosystems, USA). Cross-sections were placed in 1x TBS for 10 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... embedded in OCT tissue freezing medium and 5 μm cross-sectioned on a Cryostat (Leica CM3050 S, Leica Biosystems, USA). Cross-sections were placed in 1x TBS for 10 minutes ...
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bioRxiv - Genomics 2019Quote: ... Ten-µm-thick sections of each OCT-embedded tissue were cut using the Leica CM1950 (Leica Biosystems, Buffalo Grove, IL). Frozen slides were thawed to room temperature ...
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bioRxiv - Neuroscience 2019Quote: ... the membrane was carefully removed and an extra amount of OCT was added on top of the slice and cut on cryostate (Leica) at a thickness of 10 µm at −20 °C ...
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bioRxiv - Bioengineering 2021Quote: ... The spheroids were then imbedded in OCT compound (Fisher Healthcare, Waltham, MA, USA) and sectioned into 10 μm thick slices using a cryostat instrument (Leica CM1950 ...
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bioRxiv - Physiology 2021Quote: Serial OCT mounted cryostat sections (10 µm in thickness) were cut at -14°C (Leica Cryostat (Fisher Supply, Waltham, MA)) ...
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bioRxiv - Cell Biology 2021Quote: ... Brains were embedded and mounted in Tissue-Tek OCT compound (Sakura finetek) and 20 µm sections were cut using a cryostat (Leica). Brain sections were rehydrated in PBS ...
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bioRxiv - Microbiology 2022Quote: ... Dehydrated samples were frozen in Tissue-Tek OCT and cut into 15 μm cross sections with a CM1950 cryostat (Leica). Cryostat sections were incubated in permeabilization buffer (PBS containing 1% saponin ...
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bioRxiv - Neuroscience 2021Quote: ... Tissue was rapidly frozen using optimal cutting temperature compound (OCT) and cut into 14-µm-thick slices on a cryostat (CM 3050S, Leica). Tissue and slices were stored at -80°C.
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bioRxiv - Neuroscience 2021Quote: ... embedded in OCT, frozen and cryosectioned (coronally at 40μm, between Bregma positions 1.0mm-2.0mm) in the CM3050S cryostat (Leica Biosystems, Nussloch, DE). Brain sections were collected on adherent slides and stored at −20°C.
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bioRxiv - Neuroscience 2020Quote: ... and cryoprotected in 30% sucrose in PBS for 24-48 h before embedding in Optimum Cutting Temperature (OCT, Tissue Tek) and sectioning into 35 µm-thick coronal slices using a cryostat (CM3050, Leica). For rabies input mapping ...
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bioRxiv - Cell Biology 2021Quote: Tissues were embedded in OCT mounting media (Fisher HealthCare 23-730-571) and cut at 50 μm on a cryostat (Leica). Bones were fully decalcified in 14% EDTA ...
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bioRxiv - Cell Biology 2020Quote: Frozen sections (10μm) of muscle embedded in OCT of mid-belly level were made using a cryostat (Leica CM 1950) and collected on Superfrost Ultra Plus slides (Thermo Scientific ...
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bioRxiv - Developmental Biology 2020Quote: ... For sequence analysis fetal tissues embedded in OCT were sectioned at 10µm onto polyethylene napthalate (PEN) membrane slides (Leica, 11505158) and heart tissue was collected using a Leica LMD7000 Laser Microdissection System from CVB3-infected fetuses showing a VSD and uninfected control fetuses ...
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bioRxiv - Genomics 2020Quote: ... overnight at 4°C with gentle rocking, then cryo-embedded in Optimal Cutting Temperature compound (OCT, Sakura) and sectioned using a cryostat (Leica) onto Superfrost Plus Microscope Slides (Fisherbrand) ...