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Citations for Leica :
351 - 400 of 425 citations for Sodium hypochlorite solution cp since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Germany) to a sylgard-coated cover glass covered with bath solution (Sylgard 184, Dowcorning, USA) under stereoscopic microscope M50 (Leica, Germany). After clearing the viscera by suction through a glass pipette ...
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bioRxiv - Cell Biology 2024Quote: ... They were dehydrated in a graded series of ethanol solutions and then dried by the CO2 critical-point method using EM CPD300 (Leica Microsystems). Samples were mounted on an aluminum stub with a silver lacquer and sputtercoated with a 5 nm platinum layer using EM ACE600 (Leica Microsystems) ...
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bioRxiv - Cancer Biology 2024Quote: ... The slides were then subject to a series of solutions for deparaffinization and rehydration using a linear stainer (Leica Biosystems, ST4020): 3× xylene ...
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bioRxiv - Neuroscience 2024Quote: ... Coronal slices (300 µm) were then prepared in the same carbogenated solution by cutting the brain with a vibrotome (Leica, VT1200S) and left in carbon-gassed artificial cerebrospinal fluid (aCSF ...
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bioRxiv - Neuroscience 2023Quote: ... the first was set to circulate the solution through the heart into the vasculature by using a perfusion needle (Leica, 39471024), the second was immersed into the solution chamber where the animal was placed ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were stored in 4% formaldehyde for 24h at 4°C after which they were transferred to 30% sucrose solution at 4°C until they were cut in 50 μm thick coronal slices with a cryostat (Leica, Germany). The brain slices were washed twice for 10 minutes with phosphate buffered saline (PBS) ...
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bioRxiv - Neuroscience 2024Quote: ... Antigen retrieval was carried out with TRIS buffer at pH 9 for 20 mins at 95°C using Bond Epitope Retrieval Solution 2 (Leica Biosystems). HA and Myc tag wer detected using anti-HA (see above ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were stained with 1 µM siR-DNA solution and subsequently migratory behavior of the cells was recorded under a confocal-microscope (Leica SP8) for 24 hours ...
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bioRxiv - Neuroscience 2023Quote: ... the brain was briefly submerged in ice cold cut solution bubbled with carbogen (95% oxygen, 5% CO2) and then sectioned into 300μm sagittal sections (Leica VT1200S vibratome) in bubbled ice-cold cut solution ...
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bioRxiv - Cell Biology 2022Quote: ... They were dehydrated in a graded series of ethanol solutions and then dried by the CO2 critical-point method using EM CPD300 (Leica Microsystems). Samples were mounted on an aluminum stub with a silver lacquer and sputtercoated with a 5 nm platinum layer using EM ACE600 (Leica Microsystems) ...
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bioRxiv - Neuroscience 2022Quote: ... cryoprotected in 0.1M phosphate buffer solution containing 30% Sucrose (w/v) and cut into 50 μm thick transversal sections with a cryostat microtome (CM3050S, Leica Biosystems). Sections were counterstained with the nuclear dye 4′,6-Diamidine-2′-phenylindole dihydrochloride (DAPI ...
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bioRxiv - Neuroscience 2022Quote: ... The lumbar region of spinal cord was embedded in low-melting point agarose (4% solution, in PBS) and sectioned into 40 µm thick coronal sections with a vibrating microtome (Leica VT1000S). For KCC2 staining ...
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bioRxiv - Biochemistry 2023Quote: ... Frozen samples collected on metal carriers were transferred under liquid nitrogen into a pre-frozen cryotube containing 1 ml freeze-substitution solution (2% OsO4 in 100% acetone with 1% lecithin) and finally to a freeze substitution unit (Leica EMAFS) for processing ...
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bioRxiv - Cell Biology 2022Quote: ... using the RNAscope™ Multiplex Fluorescent V2 Assay (Cat. No. 323270) with tissue pretreatment of 15 minutes at 95°C using Bond Epitope Retrieval Solution 2 (Leica Biosystems), 15 minutes of Protease III (ACD ...
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bioRxiv - Neuroscience 2023Quote: ... The brain was rapidly placed in the same solution and 250 µm slices were prepared with a vibratome (Leica P-1000), placed in warm sucrose-based cutting solution bubbled with 95%/5% O2/CO2 (pH 7.4) ...
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bioRxiv - Neuroscience 2024Quote: ... The removed brain is immersed for 4 min in iced oxygenated sucrose solution and then placed on a cellulose nitrate membrane to separate and position the hemispheres in the vibratome (Leica VT1200s) to obtain 320 µm coronal slices (cutting speed of 0.12 mm/s) ...
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bioRxiv - Plant Biology 2024Quote: ... v/v) diluted with 100% acetone at 58°C for 20 minutes in each solution followed by a graded paraffin (Leica, USA) series (50 ...
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bioRxiv - Neuroscience 2024Quote: ... the brain was briefly submerged in ice cold cut solution bubbled with carbogen (95% oxygen, 5% CO2) and then sectioned into 300μm sagittal sections (Leica VT1200S vibratome) in bubbled ice-cold cut solution ...
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bioRxiv - Neuroscience 2024Quote: ... The brain was rapidly extracted and acute 300 μm coronal slices were cut in this solution with a vibratome (Leica VT1200S) then transferred to artificial cerebrospinal fluid (ACSF ...
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bioRxiv - Neuroscience 2024Quote: ... Slides were prepared using the frozen slide delay prior to pre-treatments using Epitope Retrieval Solution 2 (Cat No. AR9640, Leica Biosystems) at 95°C for 2 min ...
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bioRxiv - Microbiology 2024Quote: ... Sections were subjected to heat-induced epitope retrieval with citrate (Bond Epitope Retrieval Solution 1, Leica Biosystems; CD31, Ki-67, NUMA1) or EDTA (Bond Epitope Retrieval Solution 2 ...
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bioRxiv - Cancer Biology 2024Quote: ... FFPE slides were deparaffinized and subjected to heat-induced epitope retrieval at 97 ° or 100 °C using BOND epitope retrieval solutions ER1 or ER2 (Leica Biosystems). The tissue sections were incubated with a blocking solution for 10 minutes ...
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bioRxiv - Developmental Biology 2024Quote: ... the samples were transferred to 30% Sucrose solution overnight at 4°C before OCT (Sakura) inclusion for performing 20 µm coronal sections using Cryostat (Leica Biosystems). Cryostat sections were washed three times with PBST (1x PBS with Tween 20 ...
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bioRxiv - Cancer Biology 2021Quote: Unstained sections from the study cases were heated for 30 minutes in Bond™ Epitope Retrieval Solution 2 (No. AR9640; Leica Biosystems) using Leica BOND-MAXTM (Leica Microsystems) ...
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bioRxiv - Neuroscience 2020Quote: ... Coronal brain slices containing the hippocampal formation (400 μm thick) were prepared in the ice-cold cutting solution bubbled with 95% O2/5% CO2 using Vibratome VT1200S (Leica Microsystems, Germany). The slices were transferred into an interface chamber in ACSF containing (in mM) ...
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bioRxiv - Neuroscience 2021Quote: ... The brain was quickly removed into ice cold cutting solution and coronal slices containing the dorsal hippocampus were cut using a Leica vibratome (VT1200S, Leica, Wetzlar, Germany) and placed in a submerged-type chamber in ACSF containing (in mM) ...
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bioRxiv - Bioengineering 2020Quote: ... The spatial distribution of GO in the GO product and ink solutions was evaluated under a Zoom Stereomicroscope (Leica Microsystems, Heerbrugg, Switzerland).
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bioRxiv - Neuroscience 2021Quote: ... Mice were decapitated and their brains were carefully removed for coronal slice preparation (350 μm thick) in cold NMDG solution using a VT1200s microslicer (Leica Microsystems Co.). Acute slices were collected and transferred to a chamber filled with artificial cerebrospinal fluid (ACSF ...
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bioRxiv - Neuroscience 2021Quote: ... brains were then transferred to a 30 % sucrose solution for at least 3 days and coronal sections (200 µm) containing hippocampus were obtained using a vibratome (Leica vt 1000S). Sections were collected into 24-wells plate in sucrose 6% for one day stored at 4°C ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... glued to a stage and 400 μm coronal whole-brain slices were prepared while submerged in cold cutting solution using a vibrotome VT1000S (Leica, Wetzlar, DEU). Slices were recovered for 40 min at 32 °C in saturated ACSF (cutting solution with 2.5 mM CaCl2 and 1.3 mM MgCl ...
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bioRxiv - Neuroscience 2021Quote: ... The brain was quickly removed and slices were prepared in cutting solution using a vibratome (VT1000A, Leica, Leica Biosystems GmbH, Nussloch, Germany). Slices were incubated in a submerged holding chamber in ACSF at 35 °C for 30 min and then stored in the same chamber at room temperature.
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bioRxiv - Microbiology 2022Quote: ... Cell pellets were transferred to specimen carriers and immediately frozen in the presence of 20% w/v bovine serum albumin (BSA) solution using a high-pressure freezer Leica EM ICE (Leica Microsystems, Austria). Freeze substitution was performed in the presence of 2% osmium tetroxide diluted in 100% acetone at −90 °C ...
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bioRxiv - Biochemistry 2020Quote: ... seedlings were mounted on slides with ClearSee solution for imaging with both a Leica SP5 confocal laser scanning microscope (Leica, Wetzlar, Germany) and an Olympus Fluoview FV1000 (Olympus ...
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bioRxiv - Developmental Biology 2020Quote: ... Finally, embryos were cleared in BABB (benzyl alcohol: benzyl benzoate, 1:2) solution and vizualized under light microscope (Leica MZ6 Infinity1 stereomicroscope).
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bioRxiv - Neuroscience 2023Quote: ... A single 250 μm-thick parasagittal slice was cut for each mouse in this ice-cold solution using a sapphire blade (Delaware Diamond Knives, Wilmington, DE) on a vibratome (VT1000S; Leica, Deerfield, IL). Slices were then recovered at 32°C for 20-30 minutes in oxygenated artificial cerebrospinal fluid (ACSF ...
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bioRxiv - Plant Biology 2023Quote: ... T1 seeds from plants transformed with pSS01_35Spro:LIP1-GFP and pB7WG2_35Spro:LIP1 were harvested and selected either with 240 mg/L glufosinate ammonium solution (Basta) or screened for fluorescence on a stereomicroscope (Leica M165 FC) equipped with a GFP filter (excitation ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were then frozen in methylbutane solution (−50 to −60 °C) and sectioned into 40 μm serial sections with a freezing microtome (Leica SM 2010R). Serial sections spaced 240 μm apart representing the entire forebrain were collected in a 6-well tray using a freezing microtome (Stoyka et al. ...
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bioRxiv - Physiology 2023Quote: ... the worms were randomly chosen and treated with 10 mM sodium azide in M9 solution and aligned on an 2% agarose pad on slides and images acquired using a confocal microscope (Leica TCS SPE) with a 63× objective lens ...
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bioRxiv - Neuroscience 2023Quote: ... Coronal slices containing DCN were cut at 210 µm in ice-cold sucrose solution on a vibratome ((VT1200S; Leica Microsystems, Wetzlar, Germany) or (7000smz-2 ...
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bioRxiv - Molecular Biology 2024Quote: Pollen grains were mounted on slides in Alexander’s staining solution [Alexander 1969] and examined under the microscope (MZFL III Leica Microsystems, Wetzlar, Germany). Viable and non-viable pollen grains were observed in three independent experiments.
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bioRxiv - Neuroscience 2023Quote: ... Coronal slices of 350-400 µm thickness were made in ice-cold cutting solution using a VT1200 vibratome (Leica Microsystems, Wetzlar, Germany). The slices were transferred to a submerged holding chamber containing the artificial cerebrospinal fluid (aCSF ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... The brains were then transferred to a 30% sucrose solution and finally sectioned coronally (40 μm) using a cryostat (Leica Biosystems, Switzerland). The sectioned slices were immediately immersed in cryoprotectant solution and stored at −20°C.
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bioRxiv - Plant Biology 2023Quote: ... mounted on slides in Hoyer’s solution 69 and the abaxial side was examined using a Leica DM5000B microscope (Leica Microsystems, Wetzlar, Germany). Typically ...
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bioRxiv - Molecular Biology 2023Quote: ... Slides were baked at 60 °C for 15 min and deparaffinized with the Leica Bond Dewax solution (Leica Biosystems, Deer Park, IL), followed by heat-based antigen retrieval using Bond Epitope Retrieval Solution 1 (Leica Biosystems ...
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bioRxiv - Neuroscience 2023Quote: ... then sectioned into 250 μm-thick slices in the oxygenated ice-cold cutting solution using a sapphire blade (Delaware Diamond Knives, Wilmington, DE) on a vibratome (VT1200S; Leica, Deerfield, IL). The slices collected were allowed to recover at 30°C for 20 minutes in oxygenated saline solution (in mM) ...
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bioRxiv - Developmental Biology 2024Quote: ... PFA-fixed and paraffin embedded tissue slides (5 µm) were incubated for 1h at 60°C with Bond Dewax solution (Leica Bond, AR9222) to deparaffinize tissue sections ...
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bioRxiv - Biophysics 2024Quote: A small portion of the isolated chromosomes was stained with DAPI and imaged in solution on a glass bottom dish with an inverted fluorescence microscope (Leica DMi8 or Leica DMI6000B, both with Leica DFC9000GT camera) to assess yield and check for retained chromosome morphology and kinetochore fluorescence ...
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bioRxiv - Synthetic Biology 2024Quote: ... the fixed samples were dehydrated in a graded series of water-ethanol solutions ranging from 30% to 100% ethanol and dried by a critical point dryer (Leica EM CPD030). The top surface of the BC pellicle was coated with platinum under vacuum and observed via a scanning electron microscopy (Hitachi SU8010 ...
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bioRxiv - Neuroscience 2024Quote: ... Contrast enhancement was performed using a double staining protocol with uranyl acetate and lead citrate solutions (EM AC20; Leica Microsystems, Wetzlar, Germany).
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bioRxiv - Neuroscience 2024Quote: ... Sagittal slices of vermal cerebellar tissue were cut 250µm thick in an ice-cold slicing solution using a vibratome (VT1000S, Leica Biosystems, Wetzlar, Germany) with a ceramic blade (Campden Instruments Ltd ...